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Microengines have shown promise for a variety of applications in nanotechnology, microfluidics, and nanomedicine, including targeted drug delivery, microscale pumping, and environmental remediation. However, achieving precise control over their dynamics remains a significant challenge. In this study, we introduce a microengine that exploits both optical and thermal effects to achieve a high degree of controllability. We find that in the presence of a strongly focused light beam, a gold-silica Janus particle becomes confined at the stationary point where the optical and thermal forces balance. By using circularly polarized light, we can transfer angular momentum to the particle, breaking the symmetry between the two forces and resulting in a tangential force that drives directed orbital motion. We can simultaneously control the velocity and direction of rotation of the particle changing the ellipticity of the incoming light beam while tuning the radius of the orbit with laser power. Our experimental results are validated using a geometrical optics phenomenological model that considers the optical force, the absorption of optical power, and the resulting heating of the particle. The demonstrated enhanced flexibility in the control of microengines opens up new possibilities for their utilization in a wide range of applications, including microscale transport, sensing, and actuation., Competing Interests: The authors declare no competing financial interest., (© 2023 The Authors. Published by American Chemical Society.)

The preparation and characterization of a hybrid composite, based on carbon cloth (CC) matrix functionalized with two-dimensional (2D) MoS 2 flakes and MoO 3 , and its use for developing an electrochemical sensor for the determination of riboflavin (RF) is here reported. The 2D-MoS 2 -MoO 3 CC composite was prepared by depositing 2D-MoS 2 nanosheets, obtained by liquid phase exfoliation (LPE), on the surface of a carbon cloth fiber network, previously functionalized with a layer of molybdenum oxide (α-MoO 3 ) by radio-frequency magnetron reactive sputtering technique. The 2D-MoS 2 -MoO 3 CC composite was characterized by scanning electron microscopy and energy dispersive X-ray analysis (SEM-EDX), and Raman spectroscopy. An electrochemical sensor has been then fabricated by fixing a slice of the 2D-MoS 2 -MoO 3 CC composite on the working electrode of a screen-printed carbon electrode (SPCE). The 2D-MoS 2 -MoO 3 -CC/SPCE sensor display good electrochemical characteristics which have been exploited, for the first time, in the electroanalytical determination of riboflavin (RF). The sensitivity to RF, equal to 0.67 µA mM -1 in the linear range from 2 to 40 µM, and a limit of detection (LOD) of 1.5 µM at S/N = 3, demonstrate the promising characteristics of the proposed 2D-MoS 2 -MoO 3 -CC/SPCE electrochemical sensor for the determination of riboflavin.

Our understanding of the fate and distribution of micro- and nano- plastics in the marine environment is limited by the intrinsic difficulties of the techniques currently used for the detection, quantification, and chemical identification of small particles in liquid (light scattering, vibrational spectroscopies, and optical and electron microscopies). Here we introduce Raman Tweezers (RTs), namely optical tweezers combined with Raman spectroscopy, as an analytical tool for the study of micro- and nanoplastics in seawater. We show optical trapping and chemical identification of sub-20 μm plastics, down to the 50 nm range. Analysis at the single particle level allows us to unambiguously discriminate plastics from organic matter and mineral sediments, overcoming the capacities of standard Raman spectroscopy in liquid, intrinsically limited to ensemble measurements. Being a microscopy technique, RTs also permits one to assess the size and shapes of particles (beads, fragments, and fibers), with spatial resolution only limited by diffraction. Applications are shown on both model particles and naturally aged environmental samples, made of common plastic pollutants, including polyethylene, polypropylene, nylon, and polystyrene, also in the presence of a thin eco-corona. Coupled to suitable extraction and concentration protocols, RTs have the potential to strongly impact future research on micro and nanoplastics environmental pollution, and enable the understanding of the fragmentation processes on a multiscale level of aged polymers.

Silicon nanowires are held and manipulated in controlled optical traps based on counter-propagating beams focused by low numerical aperture lenses. The double-beam configuration compensates light scattering forces enabling an in-depth investigation of the rich dynamics of trapped nanowires that are prone to both optical and hydrodynamic interactions. Several polarization configurations are used, allowing the observation of optical binding with different stable structure as well as the transfer of spin and orbital momentum of light to the trapped silicon nanowires. Accurate modeling based on Brownian dynamics simulations with appropriate optical and hydrodynamic coupling confirms that this rich scenario is crucially dependent on the non-spherical shape of the nanowires. Such an increased level of optical control of multiparticle structure and dynamics open perspectives for nanofluidics and multi-component light-driven nanomachines.

Optical forces are used to push and aggregate gold nanorods onto several substrates creating surface-enhanced Raman scattering (SERS) active hot spots for Raman-based identification of proteins. By monitoring the increase of the protein SERS signal, we observe different aggregation times for different curvatures of the substrates. The slower aggregation dynamics on curved surfaces is justified by a simple geometrical model. In particular, this technique is used to decorate three-dimensional microstructures and to quickly realize hybrid micro/nanosensors for highly sensitive detection of biological material directly in a liquid environment.

Optical forces are used to aggregate plasmonic nanoparticles and create SERS-active hot spots in liquid. When biomolecules are added to the nanoparticles, high sensitivity SERS detection can be accomplished. Here, we pursue studies on Bovine Serum Albumin (BSA) detection, investigating the BSA-nanorod aggregations in a range from 100 µM to 50 nM by combining light scattering, plasmon resonance and SERS, and correlating the SERS signal with the concentration. Experimental data are fitted with a simple model describing the optical aggregation process. We show that BSA-nanorod complexes can be optically printed on non-functionalized glass surfaces, designing custom patterns stable with time. Furthermore, we demonstrate that this methodology can be used to detect catalase and hemoglobin, two Raman resonant biomolecules, at concentrations of 10 nM and 1 pM, respectively, i.e., well beyond the limit of detection of BSA. Finally, we show that nanorods functionalized with specific aptamers can be used to capture and detect Ochratoxin A, a fungal toxin found in food commodities and wine. This experiment represents the first step towards the addition of molecular specificity to this novel biosensor strategy., Competing Interests: The authors declare no conflict of interest.

In recent years, considerable effort has been devoted to the synthesis and characterization of two-dimensional materials. Liquid phase exfoliation (LPE) represents a simple, large-scale method to exfoliate layered materials down to mono- and few-layer flakes. In this context, the contactless trapping, characterization, and manipulation of individual nanosheets hold perspectives for increased accuracy in flake metrology and the assembly of novel functional materials. Here, we use optical forces for high-resolution structural characterization and precise mechanical positioning of nanosheets of hexagonal boron nitride, molybdenum disulfide, and tungsten disulfide obtained by LPE. Weakly optically absorbing nanosheets of boron nitride are trapped in optical tweezers. The analysis of the thermal fluctuations allows a direct measurement of optical forces and the mean flake size in a liquid environment. Measured optical trapping constants are compared with T-matrix light scattering calculations to show a quadratic size scaling for small size, as expected for a bidimensional system. In contrast, strongly absorbing nanosheets of molybdenum disulfide and tungsten disulfide are not stably trapped due to the dominance of radiation pressure over the optical trapping force. Thus, optical forces are used to pattern a substrate by selectively depositing nanosheets in short times (minutes) and without any preparation of the surface. This study will be useful for improving ink-jet printing and for a better engineering of optoelectronic devices based on two-dimensional materials.

We study the rotational dynamics of solid chiral and birefringent microparticles induced by elliptically polarized laser light in optical tweezers. We find that both reflection of left circularly polarized light and residual linear retardance affect the particle dynamics. The degree of ellipticity of laser light needed to induce rotations is found. The experimental results are compared with analytical calculations of the transfer of angular moment from elliptically polarized light to chiral birefringent particles.

Strategies for in-liquid molecular detection via Surface Enhanced Raman Scattering (SERS) are currently based on chemically-driven aggregation or optical trapping of metal nanoparticles in presence of the target molecules. Such strategies allow the formation of SERS-active clusters that efficiently embed the molecule at the "hot spots" of the nanoparticles and enhance its Raman scattering by orders of magnitude. Here we report on a novel scheme that exploits the radiation pressure to locally push gold nanorods and induce their aggregation in buffered solutions of biomolecules, achieving biomolecular SERS detection at almost neutral pH. The sensor is applied to detect non-resonant amino acids and proteins, namely Phenylalanine (Phe), Bovine Serum Albumin (BSA) and Lysozyme (Lys), reaching detection limits in the μg/mL range. Being a chemical free and contactless technique, our methodology is easy to implement, fast to operate, needs small sample volumes and has potential for integration in microfluidic circuits for biomarkers detection.

Optical trapping of silver nanoplatelets obtained with a simple room temperature chemical synthesis technique is reported. Trap spring constants are measured for platelets with different diameters to investigate the size-scaling behaviour. Experimental data are compared with models of optical forces based on the dipole approximation and on electromagnetic scattering within a T-matrix framework. Finally, we discuss applications of these nanoplatelets for surface-enhanced Raman spectroscopy.

Chirality is one of the most prominent and intriguing aspects of nature, from spiral galaxies down to aminoacids. Despite the wide range of living and non-living, natural and artificial chiral systems at different scales, the origin of chirality-induced phenomena is often puzzling. Here we assess the onset of chiral optomechanics, exploiting the control of the interaction between chiral entities. We perform an experimental and theoretical investigation of the simultaneous optical trapping and rotation of spherulite-like chiral microparticles. Due to their shell structure (Bragg dielectric resonator), the microparticles function as omnidirectional chiral mirrors yielding highly polarization-dependent optomechanical effects. The coupling of linear and angular momentum, mediated by the optical polarization and the microparticles chiral reflectance, allows for fine tuning of chirality-induced optical forces and torques. This offers tools for optomechanics, optical sorting and sensing and optofluidics.

The mid-range structure of glasses of the glass-forming system xNb(2)O(5)-(60 - x)P(2)O(5)-40Na(2)O, with x varying from 0 to 40, has been investigated by Raman spectroscopy and electro-optical Kerr measurements. It was found that the mid-range inhomogeneities in the glasses under study vary with x and their compositions and structures dramatically differ from the gross glass composition. It is shown that inhomogeneities with the 8NaNbO(3) + (Nb(2)O(5)·P(2)O(5)) composition are responsible for the electro-optical sensitivity of the glasses. The electro-optical structural elements of the inhomogeneities of this type are assumed to be fragments of the quasicubic lattice of crystalline NaNbO(3) demonstrating a rather high electro-optic Kerr coefficient. The obtained results are discussed in terms of the concepts of constant stoichiometric groupings and crystal motifs.

The recognition of some globular proteins was carried out in aqueous solution, at micromolar concentrations, by using an uncharged symmetrical cobalt-porphyrin (Co-P). By means of UV/Vis, induced circular dichroism, and fluorescence spectroscopy techniques, it was ascertained that the interactions between specific amino acid residues and Co-P occurred on the protein surface. In particular, spectroscopic evidence showed the formation of supramolecular complexes without disruption of the native structure of the proteins and, furthermore, that signal changes were characteristic of each Co-P/protein system, so that they could be used as a highly sensitive analytical tool for protein recognition. The relative association constants were proportional to the protein molecular masses (and then to the number of amino acid residues)., (Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

We use laser beams with radial and azimuthal polarization to optically trap carbon nanotubes. We measure force constants and trap parameters as a function of power showing improved axial trapping efficiency with respect to linearly polarized beams. The analysis of the thermal fluctuations highlights a significant change in the optical trapping potential when using cylindrical vector beams. This enables the use of polarization states to shape optical traps according to the particle geometry, as well as paving the way to nanoprobe-based photonic force microscopy with increased performance compared to a standard linearly polarized configuration.

Silicon nanoparticles obtained by ball-milling of a 50% porosity silicon layer have been optically trapped when dispersed in a water-surfactant environment. We measured the optical force constants using linearly and radially polarized trapping beams finding a reshaping of the optical potential and an enhanced axial spring constant for the latter. These measurements open perspectives for the control and handling of silicon nanoparticles as labeling agents in biological analysis and fluorescence imaging techniques.

Objectives: To determine whether changes related to age in gastroesophageal reflux (GER) in infants and children are due to acid, non acid reflux or both, as determined by 24 hr pH probe (pH) and Multichannel Intraluminal Impedance (MII)., Methods: Tracings of simultaneous pH-MII from 243 infants and children who presented with either digestive or respiratory symptoms attributable to GER were reviewed and analyzed using Mann-Whitney U test., Results: The number of GER episodes recorded was similar among children with predominantly gastrointestinal and those with respiratory symptoms. A significantly higher total number of GER episodes was observed by pH probe and MII in children under 22.8 mos of age compared with those who were older (median 159 vs. 110.5, p = 0.002). There was no significant change with age of acid reflux (AR) parameters. The changes observed were due to the significant decrease of non AR for all parameters measured, regardless of the presenting symptom., Conclusions: The decrease in GER parameters that is observed after a mean of 22.8 mos. of age is at the expense of non AR. This finding may have an impact on the choice and results of therapeutic modalities in children versus that in infants.

UV/vis absorption and time-resolved fluorescence measurements on alcoholic solutions of meso-tetrakis(4-sulfonatophenyl)porphyrin (TPPS(4)) in neutral and acid form have been performed as a function of the alcohol polarity. These solutions show a peculiar behavior that mimics porphyrin in confined water solutions. In alcohols, TPPS(4) exhibits a metastable phase showing the formation of new species in analogy with the confined water environment of AOT microemulsions. Various species have been detected at different pH values and on aging the solutions. Under neutral pH conditions, the porphyrin is present as free base monomer (S415) with a small amount of H-dimeric species (S400). On aging, the S415 leads to the formation of a new species (S423), which has been assigned as a J-type dimer of the neutral porphyrin. The species S400 and S423 are not present in bulk water solution but are typical of TPPS(4) in confined water. On decreasing pH, the S415 almost immediately converts into the diacid form (S438), which evolves toward red-shifted J-aggregates (S490) and blue-shifted H-aggregates (S420). On decreasing alcohol polarity, the kinetic evolution from fresh to aged solution and from the monomeric diacid species to H- and J-aggregates speeds up. Exploiting the amphiphilic character of short chain alcohols and widely varying their polarity, we were able to enhance in bulk conditions the peculiar behavior observed in close proximity to the microemulsion wall.

Catalytic activity of iron based catalysts in the production of multi-walled carbon nanotubes (MWCNTs) has been investigated. The effect of the carbon source (ethane or isobutane), catalyst support (Al2O3 or SiO2), iron loading, catalyst reduction temperature and reaction temperature on yield and quality of carbon products has been examined. The structural and morphological properties of catalyst and carbon products obtained have been analyzed by means of scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), Raman spectroscopy (RS), thermogravimetric analysis (TGA) and X-ray powder diffraction (XRD). The iron-based catalysts supported on alumina seem to be efficient systems for the production of carbon nanotubes from chemical vapor deposition (CVD) of isobutane with very interesting yields. The opportune calibration of reaction parameters, such as iron loading and reaction temperature, can in fact drive the synthesis toward the formation of high quality CNTs.

Ibuprofen, indomethacin, ketoprofen, piroxicam and diclofenac have been quantified in dragees, suspension, suppositories, capsules, injection solutions and tablets by capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC). The experiments were performed without specific sample pretreatment. The reproducibility of the method was investigated. Good quantitation was obtained in short analysis times. CE and MEKC are found to offer a good alternative to conventional HPLC methods.

The possibilities of capillary electrophoresis (CE) and micellar electrokinetic capillary chromatography (MEKC) were investigated for the qualitative analysis of some non-steroidal antiinflammatory drugs. In CE the influence of the pH of the buffer and its ionic strength were investigated for a test mixture of six compounds. Also the influence of organic modifiers was studied. The best conditions were applied to the separation of 15 drugs. In MEKC the influence of the concentration of SDS in buffers with pH ranges of 8.0-9.0 was investigated. The influence of an organic modifier, namely acetonitrile was discussed, whereby an interesting phenomenon of change in retention behaviour was noted. A combination of CE and MEKC allows the separation of the 15 above-mentioned compounds and forms an interesting alternative to HPLC.