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156 results on '"Dong, Juane"'

16. Sea buckthorn polysaccharide ameliorates high-fat diet induced mice neuroinflammation and synaptic dysfunction via regulating gut dysbiosis

Author

Lan, Ying, primary, Ma, Zhiyuan, additional, Chang, Lili, additional, Peng, Jing, additional, Zhang, Mengqi, additional, Sun, Qingyang, additional, Qiao, Ruixue, additional, Hou, Xinglin, additional, Ding, Xuechao, additional, Zhang, Qiang, additional, Peng, Qiang, additional, Dong, Juane, additional, and Liu, Xuebo, additional

Published
2023
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26. The SmMYB36-SmERF6/SmERF115 module regulates the biosynthesis of tanshinones and phenolic acids in salvia miltiorrhiza hairy roots.

Author

Li, Qi, Fang, Xin, Zhao, Ying, Cao, Ruizhi, Dong, Juane, and Ma, Pengda

Subjects
SALVIA miltiorrhiza, PHENOLIC acids, BIOSYNTHESIS, GENETIC regulation, METABOLISM, TRANSCRIPTION factors, ERGOT alkaloids
Abstract

Tanshinone and phenolic acids are the most important active substances of Salvia miltiorrhiza, and the insight into their transcriptional regulatory mechanisms is an essential process to increase their content in vivo. SmMYB36 has been found to have important regulatory functions in the synthesis of tanshinone and phenolic acid; paradoxically, its mechanism of action in S. miltiorrhiza is not clear. Here, we demonstrated that SmMYB36 functions as a promoter of tanshinones accumulation and a suppressor of phenolic acids through the generation of SmMYB36 overexpressed and chimeric SmMYB36-SRDX (EAR repressive domain) repressor hairy roots in combination with transcriptomic-metabolomic analysis. SmMYB36 directly down-regulate the key enzyme gene of primary metabolism, SmGAPC, up-regulate the tanshinones biosynthesis branch genes SmDXS2, SmGGPPS1, SmCPS1 and down-regulate the phenolic acids biosynthesis branch enzyme gene, SmRAS. Meanwhile, SmERF6, a positive regulator of tanshinone synthesis activating SmCPS1, was up-regulated and SmERF115, a positive regulator of phenolic acid biosynthesis activating SmRAS, was down-regulated. Furthermore, the seven acidic amino acids at the C-terminus of SmMYB36 are required for both self-activating domain and activation of target gene expression. As a consequence, this study contributes to reveal the potential relevance of transcription factors synergistically regulating the biosynthesis of tanshinone and phenolic acid. [ABSTRACT FROM AUTHOR]

Published
2023
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36. Additional file 2 of Genome-wide characterization and expression profiling of MAPK cascade genes in Salvia miltiorrhiza reveals the function of SmMAPK3 and SmMAPK1 in secondary metabolism

Author

Yongfeng Xie, Meiling Ding, Zhang, Bin, Yang, Jie, Tianlin Pei, Pengda Ma, and Dong, Juane

Abstract

Additional file 2 :Figure S1. Conserved motifs in the products of genes belonging to the SmMAPK family, as predicted by MEME software. Figure S2. A proposed interaction network involving the products of genes belonging to the SmMAPK family. Figure S3. Pearson correlation coefficient of R value between the Ct value of the qRT-PCR results and the log2 RPKM values from the RNA-seq analysis. Pearson correlation coefficient of R values were visualized by TBtools.

Published
2020
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45. Suppression of cervical cancer cell survival by ursolic acid extracted from Catalpa bungei leaves.

Author

Xu, Hongyu, Zhou, Ziyun, Dong, Juane, and Lei, Ming

Subjects
CERVICAL cancer, URSOLIC acid, CATALPA, THERAPEUTIC use of plant extracts, PHYTOCHEMICALS, ANTIOXIDANTS, ANTINEOPLASTIC agents, THERAPEUTICS
Abstract

Background: Previous phytochemical studies showed that extracts from leaves and seed oil of Catalpa plants showed antioxidant and antitumor activities. However, the active components and their roles and molecular mechanisms were still incompletely identified. Objective: We aimed to extract and identify the active fraction from Catalpa bungei leaves and investigate its underlying mechanism in suppressing cervical cancer cell survival. Materials and Methods: Extracts from C. bungei leaves with 80% methanol were separated into different fractions. The component with optimal inhibitory effect on HeLa cell growth was isolated, identified, and examined. Results: Extracts from C. bungei leaves with methanol were separated into petroleum ether, ethyl acetate (EA), n-butanol, and water fractions. The chemicals in EA fraction exhibited optimal inhibition effects, which were further separated into 29 fractions by the silica column chromatography. The compound with optimal antitumor activity was eventually determined to be ursolic acid (UA) based on the results of Sephadex column chromatography and1H nuclear magnetic resonance analysis. UA at 5.0 and 10.0 μg/mL substantially inhibited the growth and migration of HeLa cells. UA also retarded cell cycle at G0/G1 stage and promoted cell apoptosis through activating death receptor and mitochondria-associated pathways. Conclusion: UA isolated from C. bungei leaves could inhibit growth and migration and induce apoptosis in HeLa cells. Abbreviations used: UA: Ursolic acid; EA: Ethyl acetate; HPV: Human papillomavirus, PARP: Poly ADP-ribose polymeras; ROS: Reactive oxygen species; PBS: Phosphate buffered saline; SRB: Sulfonyl rhodamine B; DMSO: Dimethyl sulfoxide; TLC: Thin layer chromatography; NMR: Nuclear magnetic resonance; CAS: Chemical abstracts service. [ABSTRACT FROM AUTHOR]

Published
2018
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