32 results on '"Dong-Dong Zheng"'
Search Results
2. Robust Nonlinear MPC With Variable Prediction Horizon: An Adaptive Event-Triggered Approach.
- Author
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Peng-Biao Wang, Xuemei Ren, and Dong-Dong Zheng 0001
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- 2023
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3. Intelligent control for robotic manipulator with adaptive learning rate and variable prescribed performance boundaries.
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Dong-Dong Zheng 0001, Xianyan Li, Xuemei Ren, and Jing Na
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- 2023
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4. Data-Driven Distributed Online Learning Control for Islanded Microgrids.
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Dong-Dong Zheng 0001, Seyed Sohail Madani, and Alireza Karimi
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- 2022
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5. Optimal Bounded Ellipsoid Identification With Deterministic and Bounded Learning Gains: Design and Application to Euler-Lagrange Systems.
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Kai Guo, Dong-Dong Zheng 0001, and Jianyong Li
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- 2022
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6. Indirect adaptive control of multi-input-multi-output nonlinear singularly perturbed systems with model uncertainties.
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Dong-Dong Zheng 0001, Kai Guo, Yongping Pan 0001, and Haoyong Yu
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- 2022
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7. Event-triggered resilient control for cyber-physical systems under periodic DoS jamming attacks.
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Peng-Biao Wang, Xuemei Ren, and Dong-Dong Zheng 0001
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- 2021
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8. Set-Membership Adaptive Robot Control With Deterministically Bounded Learning Gains
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Kai Guo, Zekun Zhang, Dong-Dong Zheng, and Jie Sun
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Control and Systems Engineering ,Electrical and Electronic Engineering ,Computer Science Applications ,Information Systems - Published
- 2022
9. Effect of thermal deformation on microstructure and properties of TC18 titanium alloy produced by laser additive manufacturing
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Yu-ting Liu, Shu-quan Zhang, Yanyan Zhu, Dong-dong Zheng, Xiao-dong Li, Hong-fang Wang, and Chang-yue Qiu
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010302 applied physics ,Materials science ,Alloy ,0211 other engineering and technologies ,Metals and Alloys ,Titanium alloy ,02 engineering and technology ,engineering.material ,Deformation (meteorology) ,Laser ,Microstructure ,01 natural sciences ,law.invention ,Mechanics of Materials ,law ,0103 physical sciences ,Materials Chemistry ,engineering ,Deposition (phase transition) ,Grain boundary ,Composite material ,Ductility ,021102 mining & metallurgy - Abstract
Grain boundary of α phase damaged ductility of laser melting-deposited TC18 titanium alloy and grain boundary of α phases were difficult to break by nominal heat treatment. An extra thermal deformation was introduced to break the grain boundary of α phase with the improved mechanical property of TC18 titanium alloy fabricated by laser melting deposition technique. Results indicated that after thermal deformation, β grains in alloy seriously elongated. When sample was deformed at temperatures from 750 to 850 °C, α phase exhibited both rod and irregular morphologies with discontinuous distribution at grain boundary, and the subsequent heat treatment would lead to spheroidization of the α phase. However, after deformation at 900 °C, α phase transferred into β phase and the subsequent heat treatment would make continuous grain boundary of α phase reappear. The suitable hot deformation can effectively break the continuous grain boundary in laser melting-deposited TC18 alloy with respected improved ductility.
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- 2020
10. Characteristics and mechanisms of molybdenum(VI) adsorption by drinking water treatment residue
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Shi-sheng Wang, Ye Tianran, Mei Yang, Bo Chen, Chuan-rui Jiang, Dong-dong Zheng, and Jianjun Lian
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Residue (chemistry) ,Adsorption ,Chemistry ,Molybdenum ,Inorganic chemistry ,chemistry.chemical_element ,Water treatment - Published
- 2019
11. The effects of tumor size and postoperative radiotherapy for patients with adult low‐grade (WHO grade II) infiltrative supratentorial astrocytoma/oligodendroglioma: A population‐based and propensity score matched study
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Jian Lin, Dongdong Lin, Bo Yin, Jun-Hao Fang, Xiaolei Zhang, Lisheng Yu, Dong-Dong Zheng, Xiangyang Deng, Shangyu Xu, Nu Zhang, Hansong Sheng, Chenghui Gu, and Dan-Dong Li
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Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Multivariate analysis ,Epidemiology ,and End Results ,Oligodendroglioma ,03 medical and health sciences ,0302 clinical medicine ,Statistical significance ,propensity score match ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Propensity Score ,Original Research ,low‐grade (WHO grade II) ,Postoperative Care ,Surveillance ,business.industry ,postoperative radiotherapy ,tumor size ,Astrocytoma ,Clinical Cancer Research ,Retrospective cohort study ,Nomogram ,medicine.disease ,Tumor Burden ,astrocytoma/Oligodendroglioma ,Oncology ,030220 oncology & carcinogenesis ,Propensity score matching ,Female ,Radiology ,Neoplasm Grading ,business ,030217 neurology & neurosurgery - Abstract
Background The update of 2018 NCCN guidelines (central nervous system cancers) recommended the risk classification of postoperative patients diagnosed as adult low‐grade (WHO grade II) infiltrative supratentorial astrocytoma/oligodendroglioma (ALISA/O) should take tumor size into consideration. Moreover, the guidelines removed postoperative radiotherapy (PORT) for low risk patients. Our study aimed to explore the specific tumor size to divide postoperative patients into relatively low‐ or high risk subgroups and the effect of PORT for ALISA/O patients. Methods We conducted a retrospective study choosing 1277 postoperative ALISA/O patients from the Surveillance, Epidemiology, and End Results database. The X‐tile analysis provided the optimal cutoff point based on tumor size. The differences between surgery alone and surgery +RT groups were balanced by propensity score‐matched analysis. The multivariable analysis and the nomogram evaluated multiple prognostic factors based on cancer‐specific survival (CSS) and overall survival (OS). Results X‐tile plots defined 59 mm (P
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- 2018
12. Protective effect of Lycium barbarum polysaccharide on di-(2-ethylhexyl) phthalate-induced toxicity in rat liver
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Rui-Jing, Liu, Yong-Jian, He, Huan, Liu, Dong-Dong, Zheng, Shao-Wen, Huang, and Chun-Hong, Liu
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Oxidative Stress ,Liver ,Diethylhexyl Phthalate ,Phthalic Acids ,Animals ,Lycium ,Drugs, Chinese Herbal ,Rats - Abstract
Di-(2-ethylhexyl)-phthalate (DEHP) is the most commonly used plasticizer and it has been a ubiquitous environmental contaminant which affects health. The purpose of this study was to investigate the protective effect of the Lycium barbarum polysaccharide (LBP) at dosages of 100, 200, and 300 mg/kg bw on DEHP-induced (3000 mg/kg) toxicity in rat liver through a 28-day animal experiment. The results showed that LBP attenuated oxidative stress slightly by lowering the production of ROS and improving the activity of SOD and GSH-Px in liver and serum of DEHP treatment rats. At the same time, the levels of PXR, CYP450, CYP2E1, CYP3A1, UGT1, and GST were reduced after LBP treatment. Moreover, LBP decreased the mRNA expression of PXR, UGT1, and GST significantly. These findings suggested that LBP might ameliorate DEHP-induced liver injury by down-regulating the expression of PXR in liver, further down-regulating the downstream phase I and II detoxification enzymes, thus reducing the damage caused by DEHP. Therefore, LBP may have the potential to become an auxiliary therapeutic agent as a natural ingredient of health food.
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- 2020
13. Effect of α texture on the anisotropy of yield strength in Ti–6Al–2Zr–1Mo–1V alloy fabricated by laser directed energy deposition technique
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Zhuo Li, Huaming Wang, Renkai Li, Shuquan Zhang, Dong-dong Zheng, Bei He, Yanyan Zhu, and Xiangjun Tian
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Materials science ,Condensed matter physics ,Mechanical Engineering ,Alloy ,Titanium alloy ,Slip (materials science) ,engineering.material ,Condensed Matter Physics ,Mechanics of Materials ,Phase (matter) ,Critical resolved shear stress ,engineering ,General Materials Science ,Texture (crystalline) ,Anisotropy ,Intensity (heat transfer) - Abstract
Near α titanium alloys fabricated by laser directed energy deposition (LDED) technique always display strong anisotropies of mechanical properties even they only contain a small amount of strong-textured β phase. This work gives a detailed investigation on the effect of α texture on the anisotropy of yield strength of a laser directed energy deposited (LDEDed) Ti–6Al–2Zr–1Mo–1V (TA15) alloy by consideration of the solid phase transformation. Results indicate that the yield strength of LDEDed TA15 alloy along three directions, the building direction (VD), the horizontal direction (HD) and 45° direction (OD), shows dramatically differences of 899.7 MPa, 937.3 MPa and 1016.7 MPa. The α texture is transformed from strong β texture respected to Burgers orientation relationship with weak variant selection. The crystallographic orientation and spatial orientation of textured α phase are the key factors to dominate the yield strength of LDEDed TA15 alloy at different loading directions. Along the VD direction, strong intensity of texture is found in the easily-activated 1 ¯ 1> and 2 ¯ 0> orientation of α phase, resulting in the largest Schmid factor. However, strong intensity along the OD direction in the hardly-activated orientation for basal and prismatic slip system of α phase leads to the lowest Schmid factor. The spatial orientation of textured α phase also affects the effective slip distance, which leads to the difference of resolved shear stress in different loading directions. The coupled effect of Schimd factor and effective slip distance controlled by transformed α texture mainly results in the anisotropy of yield strength.
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- 2021
14. Transcatheter Treatment of Aortopulmonary Window with a Symmetrical Membranous Ventricular Septal Occluder
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Min Pan, Xiaofei Li, Dong-Dong Zheng, and Hai-Xia Xu
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Adult ,medicine.medical_specialty ,Heart disease ,Septal Occluder Device ,030204 cardiovascular system & hematology ,Aortopulmonary Septal Defect ,Aortopulmonary window ,03 medical and health sciences ,Imaging, Three-Dimensional ,0302 clinical medicine ,Internal medicine ,medicine.artery ,Humans ,Medicine ,Pharmacology (medical) ,Septal Occluder ,030212 general & internal medicine ,Aorta ,business.industry ,Balloon Occlusion ,medicine.disease ,Surgery ,Treatment Outcome ,Pulmonary artery ,Cardiology ,Female ,Tomography, X-Ray Computed ,Cardiology and Cardiovascular Medicine ,business - Abstract
Aortopulmonary window (APW), the presence of a communication between aorta and pulmonary artery, is a rare congenital heart disease, and surgical intervention is the standard for closure. Recently, several cases have been treated with transcatheter device occluders. Here, we report an APW patient treated successfully using a transcatheter closure with a symmetrical membranous ventricular septal occluder. We are the first to report on a case treated with this type of occluder for APW.
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- 2017
15. Controlled Hybrid-Assembly of HPV16/18 L1 Bi VLPs in Vitro
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Dong-Dong Zheng, Jin Shi, Shuming Wu, Xiao Zha, Xianghui Yu, Yuqing Wu, Bo Sun, and Yongjiang Liu
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Models, Molecular ,0301 basic medicine ,Materials science ,Pentamer ,viruses ,Nanotechnology ,complex mixtures ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Fluorescence Resonance Energy Transfer ,General Materials Science ,Thermal stability ,Amino Acid Sequence ,High potential ,Human papillomavirus 16 ,Human papillomavirus 18 ,Virion ,virus diseases ,Oncogene Proteins, Viral ,Buffer solution ,In vitro ,030104 developmental biology ,Förster resonance energy transfer ,chemistry ,Chemical engineering ,030220 oncology & carcinogenesis ,Capsid Proteins - Abstract
Based on the helix4-exchanged HPV16 L1 and HPV18 L1, HPV16 L1 Bi and HPV18 L1 Bi, we have successfully realized the controlled hybrid-assembly of HPV16/18 L1 Bi VLPs (bihybrid-VLPs) in vitro. The bihybrid-VLPs were further confirmed by fluorescence resonance energy transfer (FRET) and complex-immunoprecipitation (Co-IP) assays. The ratio of 16 L1 Bi and 18 L1 Bi in bihybrid-VLPs was verified to be 3:5 based on a modified magnetic Co-IP procedure, when mixing 1 equiv pentamer in assembly buffer solution, but it changed with conditions. In addition, the bihybrid-VLPs showed identical thermal stability as that of normal VLPs, suggesting high potential in practical applications. The present study is significant because it modified one of the vital steps of virus life cycle at the stage of virus assembly, supplying a new approach not only to deepen structural insights but also a possibility to prepare stable, low-cost, bivalent antivirus vaccine. Furthermore, the controlled hybrid-assembly of bihybrid-VLPs in vitro provides suggestions for the design of effective multivalent hybrid-VLPs, being a potential to develop broad-spectrum vaccines for the prevention of infection with multiple types of HPV.
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- 2016
16. MicroRNA-214 promotes the calcification of human aortic valve interstitial cells through the acceleration of inflammatory reactions with activated MyD88/NF-κB signaling
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Dong-Dong Zheng, Min Pan, Xiang Cao, Jia-Hai Shi, Yue Zang, Xiaofei Li, Teng Wang, Yan Wang, and Hai-Xia Xu
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Male ,medicine.medical_specialty ,Angiogenesis ,Cellular differentiation ,Inflammation ,030204 cardiovascular system & hematology ,Bone morphogenetic protein 2 ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,Cells, Cultured ,Aged ,business.industry ,NF-kappa B ,Calcinosis ,Osteoblast ,General Medicine ,Aortic Valve Stenosis ,Middle Aged ,medicine.disease ,Intercellular Adhesion Molecule-1 ,RUNX2 ,Toll-Like Receptor 4 ,MicroRNAs ,medicine.anatomical_structure ,Aortic Valve ,Case-Control Studies ,Myeloid Differentiation Factor 88 ,TLR4 ,Cardiology ,Cancer research ,Cytokines ,Female ,medicine.symptom ,Inflammation Mediators ,Cardiology and Cardiovascular Medicine ,business ,Calcification ,Signal Transduction - Abstract
Calcific aortic valve disease (CAVD) is a complex active process involving in endothelial injury, lipid infiltration, chronic inflammation, matrix remodeling, cell differentiation, progressive bone formation, and new angiogenesis. The excess inflammatory responses induced by aortic valve interstitial cells (AVICs) are one of the common pathogeneses of this disease. Although many microRNAs (miRs) have been identified to play crucial roles in the calcification process of the aortic valve, numerous miRs are still waiting to be explored. In this study, we explored the functional role of miR-214 in the inflammatory reaction and calcification of human AVICs and its underlying molecular mechanism. Alizarin red staining was used to determine the number of calcified nodules. The protein levels of ICAM-1, IL-6, IL-8, and MCP-1 detected by enzyme-linked immunosorbent assay (ELISA) were used to assess the inflammatory reaction of AVICs; expression levels of RUNX2, Msx2, and BMP2 were used to evaluate AVICs osteoblast differentiation. Results showed that the expression levels of TLR4, MyD88, NF-κB, and miR-214 were up-regulated in the blood and aortic valve tissue samples of patients with CAVD when compared with normal individuals. Knockdown of miR-214 in AVICs inhibited the secretion of IL-6, IL-8, ICAM-1, and MCP-1, while this effect was repressed when lipopolysaccharide (LPS) was added to AVICs. LPS also enhanced the effects of miR-214 in promoting the secretion of pro-inflammatory factors. Besides, up-regulation of miR-214 promoted the protein expression of MyD88 and NF-κB but had no influence on TLR4, and miR-214 could directly combine with MyD88 protein. Up-regulation of MyD88 facilitated the secretion of pro-inflammatory factors and increased calcified nodules number and accelerated the expression of RUNX2, Msx2, and BMP2. Moreover, promotion of the expressions of pro-inflammatory factors and "osteoblast-like" cell markers induced by miR-214 overexpression was abolished when MyD88 was down-regulated in AVICs. In conclusion, this study revealed that miR-214 promoted calcification by facilitating inflammatory reaction through MyD88/NF-κB signaling pathway in AVICs.
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- 2018
17. Differential Expression of MicroRNAs in Calcific Aortic Stenosis
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Dong-Dong Zheng, Min Pan, Teng Wang, Yan Wang, Jian-Hua Zhu, Jia-Hai Shi, Hai-Xia Xu, and Xiaofei Li
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0301 basic medicine ,Aortic valve ,medicine.medical_specialty ,Aortic Valve Insufficiency ,030204 cardiovascular system & hematology ,Heart valve disorder ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Calcinosis ,Internal medicine ,microRNA ,Medicine ,Humans ,Osteopontin ,biology ,business.industry ,Aortic Valve Stenosis ,medicine.disease ,Stenosis ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Aortic Valve ,biology.protein ,Cardiology ,Osteocalcin ,Aortic valve calcification ,business - Abstract
BACKGROUND Calcific aortic stenosis (CAS) is the most common heart valve disorder. To explore the underlying mechanisms, we investigated whether key microRNAs in calcified aortic valves are differentially expressed compared to those in the non-calcified valves. METHODS Calcified aortic valves from patients with aortic stenosis and non-calcified aortic valves (control) from patients with aortic insufficiency (n = 8 per group) were obtained during cardiac valve replacement surgery. The expression of miR-26a, miR-939, miR-374b*, miR-214, miR-16, miR-665, miR-130a, miR-193b, and miR-602 were evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). MiRanda and TargetScan programs were used to predict target genes, which were verified at the levels of mRNA and protein. RESULTS The expression of osteocalcin, osteopontin, Runx2, and osterix were significantly increased in the CAS group compared with the control group. The expression of miR-26a, miR-939, and miR-374b* were significantly decreased in the CAS group compared with those in the control group (p < 0.05 and p < 0.01, respectively), and the expression of miR-214 was significantly up-regulated in the CAS group compared with that in the control group (p < 0.01). No significant differences in the expression of miR-16, miR-665, miR-130a, miR-193b, and miR602 were observed between these two groups. TWIST1 was confirmed as a target for miR-214 and expression was decreased in the CAS group compared with that in the control group. CONCLUSIONS MiR-26a, miR-939, and miR-374b* expression was decreased and miR-214 was increased in the calcified aortic valves of CAS patients. miR-214 may promote aortic valve calcification by repressing TWIST1 expression.
- Published
- 2017
18. Electron–phonon coupling in heavily electron-doped bulk FeSe: a first-principles investigation
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Miao Gao, Dong-Dong Zheng, and Xun-Wang Yan
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010302 applied physics ,Superconductivity ,Coupling ,Materials science ,Condensed matter physics ,Doping ,General Engineering ,Electron doping ,General Physics and Astronomy ,Fermi surface ,Electron phonon coupling ,02 engineering and technology ,Electron doped ,021001 nanoscience & nanotechnology ,01 natural sciences ,0103 physical sciences ,0210 nano-technology - Abstract
We present the results of electron–phonon coupling (EPC) in heavily electron-doped FeSe, based on the first-principles calculation and Wannier interpolation. It is found that the EPC constant λ can be improved by 31% at a doping level of 0.4 e/cell, with respect to the pristine case. The change of Fermi surface nesting function around M point dominates the enhancement of λ. Nevertheless, the superconducting T c of FeSe is only 0.01 mK at 0.4 e/cell doping, determined through McMillian–Allen–Dynes formula. Our findings clarify that electron doping alone can not raise the T c to the experimental value within phonon-mediated picture.
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- 2018
19. M1 macrophages promote aortic valve calcification mediated by microRNA-214/TWIST1 pathway in valvular interstitial cells
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Xiao-Fei, Li, Yan, Wang, Dong-Dong, Zheng, Hai-Xia, Xu, Teng, Wang, Min, Pan, Jia-Hai, Shi, and Jian-Hua, Zhu
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Original Article - Abstract
Objective: The identification of the biological function of M1 macrophages and the mechanism underlying their role in valvular interstitial cell (VIC) calcification may provide therapeutic targets for the prevention of aortic valve calcification (AVC). This study investigated the mechanism by which M1 macrophages and macrophage-derived microvesicles (MVs) affected the calcification of VICs. An additional aim was to investigate the involvement of the miR-214 pathway in this process. Methods: The M1 or M2 macrophage phenotype in human calcific aortic valve was confirmed by gene expression analysis of M1 or M2 macrophage markers. Two macrophage cell lines (BMDMs and RAW 264.7 macrophages) were transformed into M1 macrophages by lipopolysaccharide (LPS) stimulation. To investigate the mechanism by which M1 macrophages promoted VIC calcification, the generated M1 macrophages and macrophage-derived MVs were co-cultured with VICs and VICs were then used for calcification or signals analysis. In addition, a hypercholesterolemic apoE-/- AVC murine model was used to evaluate the therapeutic efficacy of miR-214 specific-siRNA (miR-214 inhibitor). Results: Macrophages in calcific aortic valves showed M1-directed polarization. In the VICs co-cultured with LPS-stimulated M1 macrophages and macrophage-derived MVs, VIC calcification was enhanced, and the expression of TWIST1, a direct target of miR-214, was downregulated. We showed that knockdown of TWIST1 serves as a responding molecule for miR-214 and reversed the anti-calcification action of miR-214 inhibitor, mediating signal delivery by the M1 macrophage-derived MVs to VICs and promoting VIC calcification. When M1 macrophages co-cultured with VICs, TWIST1 overexpression in M1 macrophages had no effect on the expression of TWIST1 in VICs. As shown by intravenous therapy, knockdown of miR-214 in mice seemed to improve AVC in apoE-/- mice with high-cholesterol (HC)-diet induced AVC. Conclusions: These findings suggested that M1 macrophages promoted AVC by the delivery of miR-214 to valvular interstitial cells via macrophage-derived MVs and subsequent downregulation of TWIST1 of valvular interstitial cells.
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- 2016
20. Two novel mutations of the MYBPC3 gene identified in Chinese families with hypertrophic cardiomyopathy
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Xiang-Jun Yang, Jia Lin, Xun Li, Dong-Dong Zheng, Ting-Bo Jiang, Jian-Ping Song, Junhua Yang, Wen-Ping Jiang, and Qin Tao
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Adult ,Male ,China ,Gene mutation ,medicine.disease_cause ,Polymerase Chain Reaction ,Frameshift mutation ,Exon ,Genotype-phenotype distinction ,Clinical Studies ,Genotype ,Humans ,Medicine ,Genetic Predisposition to Disease ,Gene ,Aged ,Genetics ,Mutation ,business.industry ,Cardiomyopathy, Hypertrophic ,Middle Aged ,Phenotype ,Pedigree ,Female ,Carrier Proteins ,Cardiology and Cardiovascular Medicine ,business - Abstract
Background Hypertrophic cardiomyopathy (HCM) is one of the most common genetic cardiovascular disorders. Mutations in the MYBPC3 gene are one of the most frequent genetic causes of HCM. Objectives To screen MYBPC3 gene mutations in Chinese patients with HCM, and analyze the correlation between the genotype and the phenotype. Methods The 35 exons of the MYBPC3 gene were amplified by polymerase chain reaction in the 11 consecutive unrelated Chinese pedigrees. The sequences of the products were analyzed and the mutation sites were determined. The clinical data of genotype-positive families were collected, and the correlation between genotype and phenotype was analyzed. Results Two mutations of the MYBPC3 gene were confirmed among 11 pedigrees. A frameshift mutation (Pro459fs) was identified in exon 17 in family H8, and a splice mutation (IVS5+5G→C) was identified in intron 5 in family H3. These two mutations were first identified in Chinese patients with familial HCM and were absent in 110 chromosomes of healthy controls. Seven known polymorphisms were found in the cohort. Conclusions Compared with what was reported abroad, the MYBPC3 gene is a common pathogenic gene responsible for HCM in Chinese patients, and the phenotypes of these two mutations in their respective families may have their own clinical characteristics.
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- 2010
21. Mutation of Arg723Gly in β-myosin heavy chain gene in five Chinese families with hypertrophic cardiomyopathy
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Ningzheng Dong, Xiang-Jun Yang, Ting-Bo Jiang, Bing-yuan Zhou, Dong-Dong Zheng, Xu-jie Cheng, Wen-Ping Jiang, Jian-Ping Song, Hong-Xia Li, Junhua Yang, and Caiming Zhao
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Genetics ,business.industry ,Cardiomyopathy ,Hypertrophic cardiomyopathy ,General Medicine ,Gene mutation ,medicine.disease ,Genotype-phenotype distinction ,Mutation Carrier ,Mutation (genetic algorithm) ,Genotype ,cardiovascular system ,medicine ,MYH7 ,cardiovascular diseases ,business - Abstract
Background Hypertrophic cardiomyopathy (HCM) is a form of cardiomyopathy with an autosomal dominant inherited disease, which is caused by mutations in at least one of the sarcomeric protein genes. Mutations in the beta-myosin heavy chain (β-MHC) are the most common cause of HCM. This study was to reveal the disease-causing gene mutations in Chinese population with HCM, and to analyze the correlation between the genotype and phenotype. Methods The exons 3 to 26 of MYH7 were amplified by PCR, and the PCR products were sequenced in five non-kin HCM patients. A 17-year-old patient was detected to be an Arg723Gly mutation carrier. Then his family was gene-screened, and the correlation between genotype and phenotype was analyzed. Results The mutation of Arg723Gly in a Chinese family with HCM was detected for the first time. With a C-G transversion in nucleotide 13 619 of the MYH7 gene, located at the essential light chain interacting region in S1, the replacement of arginine by glycine took place at amino acid residue 723. A two-dimensional echocardiogram showed moderate asymmetrical septal hypertrophy with left atria enlargement. There was no obstruction in the left ventricular outflow tract. In his family, a total of 13 individuals were diagnosed HCM and 5 of them were dead of congestive heart failure at a mean age of 66-year-old. Eight living members were all detected to carry the mutation, in which 3 developed progressive heart failure. Moreover, the heart function of the people evidently deteriorates when their age are older than 50. The mutation and the disease show co-separated. Conclusion The Arg723Gly mutation is a malignant type. In Chinese the mutation has the similar characters to the former report but has low degree malignant.
- Published
- 2006
22. Characteristics and mechanisms of molybdenum(VI) adsorption by drinking water treatment residue.
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Jian-jun Lian, Mei Yang, Bo Chen, Shi-sheng Wang, Tian-ran Ye, Dong-dong Zheng, and Chuan-rui Jiang
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DRINKING water ,WATER pollution ,MOLYBDENUM - Abstract
Molybdenum (Mo) is an important pollutant in surface water. Removal of Mo from aqueous solutions by adsorption using low-cost and environmental friendly adsorbents has gained wide acceptance. Drinking water treatment residue (DWTR) is an inevitable by-product during potable water production that has the high capability of adsorption of heavy metals. However, the characteristics and mechanisms of Mo adsorption by DWTR still remain unclear. In this study, we showed that Mo(VI) adsorption by DWTR followed the pseudo-second-order rate. The adsorption isotherms for Mo(VI) were best described by the Langmuir model. Thermodynamic parameters indicated that the adsorption process was endothermal, entropy increasing, and spontaneous. Electrostatic interaction and surface complexation were found to be major adsorption mechanisms. The adsorption rate of Mo(VI) by DWTR was dependent on pH values and lineally increased as pH decreased from 10 to 4. The estimated maximum adsorption capacity was 31.06 mg/g at 318 K at pH 2.0. Desorption experiments were also performed to evaluate the potential of DWTR regeneration. These results suggested that DWTR was a green, efficient, and recyclable adsorbent for Mo(VI) and could be used for removal and recovery of Mo from polluted environments. [ABSTRACT FROM AUTHOR]
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- 2019
- Full Text
- View/download PDF
23. Efficient inhibition of human papillomavirus 16 L1 pentamer formation by a carboxylatopillarene and a p-sulfonatocalixarene
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Ding-Yi Fu, Yuqing Wu, Ting Zhou, Xiao Zha, Yulong Sun, Li-Li Tan, Dong-Dong Zheng, Shi-Qi Ma, and Ying-Wei Yang
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Human papillomavirus 16 ,Chemistry ,Stereochemistry ,Pentamer ,Kinetics ,Metals and Alloys ,General Chemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Quaternary Ammonium Compounds ,Crystallography ,chemistry.chemical_compound ,Structure-Activity Relationship ,Monomer ,Phenols ,Calixarene ,Materials Chemistry ,Ceramics and Composites ,Structure–activity relationship ,Human papillomavirus ,Calixarenes - Abstract
Pillarenes and calixarenes showed obvious inhibition of HPV16 L1 pentamer formation via their selective binding to Arg and Lys residues at the monomer interface, which was reversible after the release of cyclic arenes. Pillarenes are more effective than calixarenes in terms of the inhibition efficiency, attributing to the different kinetics and binding affinity.
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- 2014
24. Mutations in the beta-myosin heavy chain gene in southern Chinese families with hypertrophic cardiomyopathy
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M Geng, Jian-Ping Song, Jia Lin, XJ Yang, Qin Tao, Dong-Dong Zheng, Wujun Jiang, Lian-hua Han, Haiying Li, and Junhua Yang
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Adult ,Male ,China ,Adolescent ,Cardiomyopathy ,macromolecular substances ,Biology ,Biochemistry ,Conserved sequence ,Young Adult ,Species Specificity ,Myosin ,Genotype ,medicine ,Cardiomyopathy, Hypertrophic, Familial ,Animals ,Humans ,Gene ,Conserved Sequence ,Polymorphism, Single-Stranded Conformational ,Aged ,Genetics ,Myosin Heavy Chains ,Biochemistry (medical) ,Hypertrophic cardiomyopathy ,Dilated cardiomyopathy ,Cell Biology ,General Medicine ,Sequence Analysis, DNA ,Middle Aged ,medicine.disease ,Pedigree ,Mutation ,MYH7 ,Female ,Cardiac Myosins - Abstract
In this study, 14 unrelated hypertrophic cardiomyopathy (HCM) probands were scanned by polymerase chain reaction– single-strand conformation poly morphism analysis and DNA sequencing. Three mis-sense mutations of the β-myosin heavy chain gene, MYH7, were found: valine (Val) 606 methionine (Met), arginine (Arg) 694 leucine (Leu), and Arg 723 glycine (Gly). All are reported here for the first time in Chinese subjects. The results showed that: Val606Met is an intermediate malignancy mutation; Arg694Leu is a novel mutation with a benign phenotype; and the Arg723Gly mutation is linked to malignancy – it can lead not only to HCM but also to dilated cardiomyopathy at various ages. The clinical symptoms associated with Arg723Gly emerged early and caused more severe clinical manifestation and poorer prognosis in females than in males. Mis-sense mutations were not detected in the myosin binding protein C, cardiac, cardiac troponin T type 2, or cardiac troponin I type 3 genes. The MYH7 gene may be an HCM mutation hotspot in the Chinese and have unique features in this study population.
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- 2010
25. Plasma Soluble Corin in Patients with Heart Failure
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Shenghan Chen, Yiqing Zhou, Peng Liu, Ningzheng Dong, Lizhen He, Dong-Dong Zheng, Edward F. Plow, Lin Li, Changgeng Ruan, Junhua Yang, and Qingyu Wu
- Subjects
Adult ,Male ,medicine.medical_specialty ,Adolescent ,Myocardial Infarction ,Enzyme-Linked Immunosorbent Assay ,Article ,Pathogenesis ,Age groups ,Internal medicine ,medicine ,Humans ,In patient ,Myocardial infarction ,Elisa method ,Heart Failure ,Analysis of Variance ,Plasma samples ,business.industry ,Serine Endopeptidases ,Middle Aged ,medicine.disease ,Endocrinology ,Heart failure ,Linear Models ,Biomarker (medicine) ,Female ,Cardiology and Cardiovascular Medicine ,business ,Biomarkers - Abstract
Background— Corin is a transmembrane protease that processes natriuretic peptides in the heart. Like many membrane proteins, corin is shed from the cell surface. Methods and Results— In this study, we obtained plasma samples from healthy controls and patients with heart failure (HF) and acute myocardial infarction. Soluble corin levels in plasma were measured by an ELISA method. In healthy adults (n=198), plasma corin levels were 690 pg/mL (SD, 260 pg/mL). The corin levels did not differ significantly among different age groups. In patients with HF (n=291), plasma corin levels were significantly lower compared with that of healthy controls (365 pg/mL [SD, 259]; P P P P >0.05). Conclusions— Soluble corin was detected in human plasma. Plasma corin levels were reduced significantly in patients with HF but not in those with acute myocardial infarction. Our results indicate that corin deficiency may contribute to the pathogenesis of HF and that plasma corin may be used as a biomarker in the diagnosis of HF.
- Published
- 2010
26. Mutation of Arg723Gly in beta-myosin heavy chain gene in five Chinese families with hypertrophic cardiomyopathy
- Author
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Jun-hua, Yang, Dong-dong, Zheng, Ning-zheng, Dong, Xiang-jun, Yang, Jian-ping, Song, Ting-bo, Jiang, Xu-jie, Cheng, Hong-xia, Li, Bing-yuan, Zhou, Cai-ming, Zhao, and Wen-ping, Jiang
- Subjects
Adult ,Male ,Ventricular Myosins ,Adolescent ,Myosin Heavy Chains ,Cardiomyopathy, Hypertrophic, Familial ,Mutation, Missense ,Humans ,Female ,Middle Aged - Abstract
Hypertrophic cardiomyopathy (HCM) is a form of cardiomyopathy with an autosomal dominant inherited disease, which is caused by mutations in at least one of the sarcomeric protein genes. Mutations in the beta-myosin heavy chain (beta-MHC) are the most common cause of HCM. This study was to reveal the disease-causing gene mutations in Chinese population with HCM, and to analyze the correlation between the genotype and phenotype.The exons 3 to 26 of MYH7 were amplified by PCR, and the PCR products were sequenced in five non-kin HCM patients. A 17-year-old patient was detected to be an Arg723Gly mutation carrier. Then his family was gene-screened, and the correlation between genotype and phenotype was analyzed.The mutation of Arg723Gly in a Chinese family with HCM was detected for the first time. With a C-G transversion in nucleotide 13,619 of the MYH7 gene, located at the essential light chain interacting region in S1, the replacement of arginine by glycine took place at amino acid residue 723. A two-dimensional echocardiogram showed moderate asymmetrical septal hypertrophy with left atria enlargement. There was no obstruction in the left ventricular outflow tract. In his family, a total of 13 individuals were diagnosed HCM and 5 of them were dead of congestive heart failure at a mean age of 66-year-old. Eight living members were all detected to carry the mutation, in which 3 developed progressive heart failure. Moreover, the heart function of the people evidently deteriorates when their age are older than 50. The mutation and the disease show co-separated.The Arg723Gly mutation is a malignant type. In Chinese the mutation has the similar characters to the former report but has low degree malignant.
- Published
- 2006
27. [Malignant hypertrophic cardiomyopathy caused by the Arg723Gly mutation in beta-myosin heavy chain gene in a Chinese pedigree]
- Author
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Dong-dong, Zheng, Jun-hua, Yang, Ning-zheng, Dong, Xiang-jun, Yang, Jian-ping, Song, Ting-bo, Jiang, Xu-jie, Cheng, Hong-xia, Li, Bing-yuan, Zhou, Cai-ming, Zhao, and Wen-ping, Jiang
- Subjects
Adult ,Male ,China ,Adolescent ,Genotype ,Myosin Heavy Chains ,Middle Aged ,Pedigree ,Young Adult ,Phenotype ,Asian People ,Mutation ,Cardiomyopathy, Hypertrophic, Familial ,Humans ,Female - Abstract
Hypertrophic cardiomyopathy (HCM) is a genetically and phenotypically heterogeneous disease and an Arg723Gly mutation in beta-myosin heavy chain (beta-MHC) gene was found in 3 Spanish families with malignant HCM. We detected this gene mutation in 5 Chinese pedigrees with hypertensive cardiomyopathy.Five Chinese pedigrees with HCM and 80 age-matched normal control subjects were chosen for the study. The exons in the functional regions of the beta-MHC gene were amplified with PCR and the products were sequenced, genotype and phenotype analyzed.Arg723Gly mutation was identified in exon 20 in one pedigree. In this pedigree, 13 out of 25 family members were diagnosed as HCM, 5 died of heart failure, all HCM patients in this pedigree had Arg723Gly mutation and 3 of them had NYHA III and 2 of them were diagnosed as HCM before the age of 20.Arg723Gly mutation was also one of the main disease-causing genes in Chinese familial HCM. The mutation of Arg723Gly is a malignant phenotype as shown by early progressive heart failure development and poor prognosis in this pedigree with Arg723Gly mutation.
- Published
- 2006
28. Genetic signatures of high-altitude adaptation in Tibetans.
- Author
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Jian Yang, Zi-Bing Jin, Jie Chen, Xiu-Feng Huang, Xiao-Man Li, Yuan-Bo Liang, Jian-Yang Mao, Xin Chen, Zhili Zheng, Bakshi, Andrew, Dong-Dong Zheng, Mei-Qin Zheng, Wray, Naomi R., Visscher, Peter M., Fan Lu, and Jia Qu
- Subjects
MAMMAL adaptation ,TIBETANS ,SINGLE nucleotide polymorphisms ,PHYSIOLOGICAL effects of altitudes ,NATURAL selection ,ULTRAVIOLET radiation - Abstract
Indigenous Tibetan people have lived on the Tibetan Plateau for millennia. There is a long-standing question about the genetic basis of high-altitude adaptation in Tibetans. We conduct a genome-wide study of 7.3 million genotyped and imputed SNPs of 3,008 Tibetans and 7,287 non-Tibetan individuals of Eastern Asian ancestry. Using this large dataset, we detect signals of high-altitude adaptation at nine genomic loci, of which seven are unique. The alleles under natural selection at two of these loci [methylenetetrahydrofolate reductase (MTHFR) and EPAS1] are strongly associated with blood-related phenotypes, such as hemoglobin, homocysteine, and folate in Tibetans. The folate-increasing allele of rs1801133 at the MTHFR locus has an increased frequency in Tibetans more than expected under a drift model, which is probably a consequence of adaptation to high UV radiation. These findings provide important insights into understanding the genomic consequences of high-altitude adaptation in Tibetans. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
29. ASSA13-03-24 Isolation of Rat Coronary Arterial Smooth Muscle Cells and Preliminary Study of the Characteristics of the Voltage Dependent Potassium Channel
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Xiang-Jun Yang, Jian-Ping Song, Lian-hua Han, Wen-Ping Jiang, Dong-Dong Zheng, Jia Lin, and Ting-Bo Jiang
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Nuclear magnetic resonance ,Smooth muscle ,business.industry ,Medicine ,Separation method ,Average current ,Anatomy ,Cardiology and Cardiovascular Medicine ,business ,Potassium channel ,Ion channel ,Arterial smooth muscle cells ,Voltage - Abstract
Objective To establish a stable and efficient enzyme separation method to isolation of SD rat coronary artery smooth muscle cells (CASMCs), and to study on the voltage dependent potassium ion channels (KV). Methods CASMCs were isolated by a “three-step” acute enzyme separation method and identified by immunofluorescence. KV currents were recorded by the patch-clamp technique. Results (1) An abundance of CAMSCs with good reactivity were obtained by this method. (2) At the test potential of +60 mV, the KV current average current densities were 34.35 ± 2.53 pA/pF (n = 5). After added 4-AP, the KV current average current densities were down to 3.55 ± 0.47 pA/pF at the same test potential ( P < 0.05). According to Boltzmann equation, the half maximal activation voltage was calculated as 23.66 ± 1.03 mV, and the slope value (k) was 14.44 ± 0.94 mV. Conclusions This method can acquire a large number of CASMCs which can be used on the study of ion channels.
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- 2013
30. In vitro monitoring of the formation of pentamers from the monomer of GST fused HPV 16 L1
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Chunlai Jiang, Xiao Zha, Dong-Dong Zheng, Xianghui Yu, Yuqing Wu, and Dong Pan
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Time Factors ,Pentamer ,Catalysis ,Virus ,law.invention ,chemistry.chemical_compound ,Western blot ,law ,Materials Chemistry ,medicine ,Static light scattering ,Glutathione Transferase ,Human papillomavirus 16 ,medicine.diagnostic_test ,Metals and Alloys ,General Chemistry ,Fusion protein ,Molecular biology ,Recombinant Proteins ,In vitro ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Kinetics ,Monomer ,chemistry ,Ceramics and Composites ,Recombinant DNA - Abstract
The recombinant GST fusion protein HPV 16 L1 from E. coli was proved to exist as a monomer rather than a pentamer, providing the possibility of real-time monitoring of the pentamer formation in vitro. Time-dependent kinetic studies of the process were performed for the first time by using static light scattering and western blot analysis, where the essential factors were revealed, offering a new biotechnical approach for virus control and/or the development of anti-viral agents.
- Published
- 2013
31. Efficient inhibition of human papillomavirus 16 L1 pentamer formation by a carboxylatopillarene and a p-sulfonatocalixarene.
- Author
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Dong-Dong Zheng, Ding-Yi Fu, Yuqing Wu, Yu-Long Sun, Li-Li Tan, Ting Zhou, Shi-Qi Ma, Xiao Zha, and Ying-Wei Yang
- Subjects
- *
PAPILLOMAVIRUSES , *CALIXARENES , *TRANSMISSION electron microscopy , *NUCLEAR magnetic resonance spectroscopy , *MONOMERS - Abstract
Pillarenes and calixarenes showed obvious inhibition of HPV16 L1 pentamer formation via their selective binding to Arg and Lys residues at the monomer interface, which was reversible after the release of cyclic arenes. Pillarenes are more effective than calixarenes in terms of the inhibition efficiency, attributing to the different kinetics and binding affinity. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
32. Electron–phonon coupling in heavily electron-doped bulk FeSe: a first-principles investigation.
- Author
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Dong-Dong Zheng, Miao Gao, and Xun-Wang Yan
- Abstract
We present the results of electron–phonon coupling (EPC) in heavily electron-doped FeSe, based on the first-principles calculation and Wannier interpolation. It is found that the EPC constant λ can be improved by 31% at a doping level of 0.4 e/cell, with respect to the pristine case. The change of Fermi surface nesting function around M point dominates the enhancement of λ. Nevertheless, the superconducting T
c of FeSe is only 0.01 mK at 0.4 e/cell doping, determined through McMillian–Allen–Dynes formula. Our findings clarify that electron doping alone can not raise the Tc to the experimental value within phonon-mediated picture. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
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