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2 results on '"Donna Goldsteen"'

1. Using refined methods to pick up mice: A survey benchmarking prevalence & beliefs about tunnel and cup handling.

Author

Lauren Young, Donna Goldsteen, Elizabeth A Nunamaker, Mark J Prescott, Penny Reynolds, Sally Thompson-Iritani, Sarah E Thurston, Tara L Martin, and Megan R LaFollette

Subjects
Medicine, Science
Abstract

Refined handling improves laboratory mouse welfare and research outcomes when compared to traditional tail handling, yet implementation does not seem to be widespread. Refined handling includes picking up a mouse using a tunnel or cupped hands. The aim of this study was to determine the current prevalence of and beliefs towards refined handling using the theory of planned behavior. It was predicted that refined handling prevalence is low compared to traditional handling methods, and its implementation is determined by individual and institutional beliefs. Research personnel were recruited via online convenience sampling through email listservs and social media. A total of 261 participants in diverse roles (e.g. veterinarians, managers, caretakers, researchers, etc.) responded primarily from the USA (79%) and academic institutions (61%) Participants were surveyed about their current use, knowledge, and beliefs about refined handling. Quantitative data were analyzed via descriptive statistics and generalised regression. Qualitative data were analyzed by theme. Research personnel reported low levels of refined handling implementation, with only 10% of participants using it exclusively and a median estimate of only 10% of institutional mice being handled with refined methods. Individually, participants had positive attitudes, neutral norms, and positive control beliefs about refined handling. Participants' intention to provide refined handling in the future was strongly associated with their attitudes, norms, and control beliefs (p

Published
2023
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2. Inhibition of choroidal neovascularization by intravenous injection of adenoviral vectors expressing secretable endostatin

Author

Kyoichi Takahashi, Michael Penn, Sandrina Phipps, Michele Melia, Peter L. Gehlbach, Katharine Hilary Dixon, Cheauyan T. Chen, Donna Goldsteen, Akira Ando, Diana Moffat, Keiko Mori, David J. Nesbitt, Gene Liau, Peter A. Campochiaro, Keisuke Mori, and Kim Brazzell

Subjects
genetic structures, Genetic enhancement, Transgene, Genetic Vectors, Gene Expression, Angiogenesis Inhibitors, macromolecular substances, medicine.disease_cause, Pathology and Forensic Medicine, Adenoviridae, Neovascularization, Mice, Genes, Reporter, medicine, Animals, Ocular Physiological Phenomena, Recombination, Genetic, Rous sarcoma virus, biology, Neovascularization, Pathologic, Choroid, Transfection, Genetic Therapy, biology.organism_classification, eye diseases, Peptide Fragments, Collagen Type XVIII, Endostatins, Mice, Inbred C57BL, Choroidal neovascularization, Liver, Immunology, Injections, Intravenous, Cancer research, cardiovascular system, sense organs, Collagen, medicine.symptom, Endostatin, Regular Articles
Abstract

Endostatin is a cleavage product of collagen XVIII that inhibits tumor angiogenesis and growth. Interferon alpha2a blocks tumor angiogenesis and causes regression of hemangiomas, but has no effect on choroidal neovascularization (CNV). Therefore, inhibitors of tumor angiogenesis do not necessarily inhibit ocular neovascularization. In this study, we used an intravenous injection of adenoviral vectors containing a sig-mEndo transgene consisting of murine immunoglobulin kappa-chain leader sequence coupled to sequence coding for murine endostatin to investigate the effect of high serum levels of endostatin on CNV in mice. Mice injected with a construct in which sig-mEndo expression was driven by the Rous sarcoma virus promoter had moderately high serum levels of endostatin and significantly smaller CNV lesions at sites of laser-induced rupture of Bruch's membrane than mice injected with null vector. Mice injected with a construct in which sig-mEndo was driven by the simian cytomegalovirus promoter had approximately 10-fold higher endostatin serum levels and had nearly complete prevention of CNV. There was a strong inverse correlation between endostatin serum level and area of CNV. This study provides proof of principle that gene therapy to increase levels of endostatin can prevent the development of CNV and may provide a new treatment for the leading cause of severe loss of vision in patients with age-related macular degeneration.

Published
2001

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