1. Dopamine-Imprinted Polymers: Template-Monomer Interactions, Analysis of Template Removal and Application to Solid Phase Extraction
- Author
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Mirosław Szutowski, Magdalena Bamburowicz-Klimkowska, Dorota Maciejewska, and Piotr Luliński
- Subjects
Dopamine hydrochloride isolation ,theoretical binding energy ,molecularly imprinted polymer ,template removal ,solid phase extraction ,Organic chemistry ,QD241-441 - Abstract
A dopamine-imprinted polymer (MIP) was prepared in aqueous methanolsolution at 60oC by free-radical cross-linking polymerization of methacrylic acid in thepresence of ethylene glycol dimethacrylate as the cross-linker and dopamine hydrochlorideas the template molecule. Its ability to isolate dopamine was evaluated as the basis of asolid phase extraction procedure and compared with that of a non-imprinted polymer(NIP). The binding of dopamine was 84.1% and 29.1% for MIP and NIP, respectively.Various reported post-polymerization treatments to reduce template bleeding wereexamined. In our case the lowest bleeding was achieved after applying a combinedprocedure: continuous extraction in a Soxhlet apparatus (CE), followed by microwave-assisted extraction (ME) to a level of 0.061 μg/mL. A simplified model of the template-monomer complexes allowed rationalization of monomer choice based on the heats ofcomplex formation at a PM3 level of theory.
- Published
- 2007
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