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1. Excessive Erythrocytosis Is Not Associated With Altered Iron Homeostasis in Men From the World’s Highest City

Author

Gaetano Cairo, Benoit Champigneulle, Margherita Correnti, Elena Gammella, Stefania Recalcati, Domenico Girelli, Annalisa Castagna, Anne-Kristine Meinild Lundby, Ivan Hancco, Carole Chirica, Dorra Guergour, Laura Oberholzer, Emeric Stauffer, Carsten Lundby, Aurélien Pichon, Julien V Brugniaux, Stéphane Doutreleau, Samuel Verges, and Paul Robach

Subjects
Diseases of the blood and blood-forming organs, RC633-647.5
Published
2023
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2. Exposure to a mixture of non-persistent environmental chemicals and neonatal thyroid function in a cohort with improved exposure assessment

Author

Ophélie Coiffier, Dorothy Nakiwala, Matthieu Rolland, Andres Malatesta, Sarah Lyon-Caen, Benoît Chovelon, Patrice Faure, Anne Sophie Gauchez, Dorra Guergour, Amrit K. Sakhi, Azemira Sabaredzovic, Cathrine Thomsen, Isabelle Pin, Rémy Slama, Christelle Corne, and Claire Philippat

Subjects
Thyroid hormones, Heel-prick blood spot, Bisphenols, Parabens, Phthalates, DINCH, Environmental sciences, GE1-350
Abstract

Background: In vitro and toxicological studies have shown that non-persistent environmental chemicals can perturb thyroid hormone homeostasis. Epidemiological studies with improved exposure assessment (i.e., repeated urine samples) are needed to evaluate effects of these compounds, individually or as a mixture, in humans. We studied the associations between prenatal exposure to non-persistent environmental chemicals and neonatal thyroid hormones. Methods: The study population consisted of 442 mother–child pairs from the French SEPAGES mother–child cohort recruited between July 2014 and July 2017. For each participant, four parabens, five bisphenols, triclosan, triclocarban, benzophenone-3 as well as metabolites of phthalates and of di(isononyl)cyclohexane-1,2-dicarboxylate were assessed in two pools of repeated urine samples (median: 21 spot urines per pool), collected in the 2nd and 3rd trimesters of pregnancy, respectively. Thyroid stimulating hormone (TSH) and total thyroxine (T4) levels were determined in newborns from a heel-prick blood spot. Maternal iodine and selenium were assessed in urine and serum, respectively. Adjusted linear regression (uni-pollutant model) and Bayesian Kernel Machine Regression (BKMR, mixture model) were applied to study overall and sex-stratified associations between chemicals and hormone concentrations. Results: Interaction with child sex was detected for several compounds. Triclosan, three parabens, and one phthalate metabolite (OH-MPHP) were negatively associated with T4 among girls in the uni-pollutant model. BKMR also suggested a negative association between the mixture and T4 in girls, whereas in boys the association was positive. The mixture was not linked to TSH levels, and for this hormone the uni-pollutant model revealed associations with only a few compounds. Conclusion: Our study, based on repeated urine samples to assess exposure, showed that prenatal exposure to some phenols and phthalates disturb thyroid hormone homeostasis at birth. Furthermore, both uni-pollutant and mixture models, suggested effect modification by child sex, while, to date underlying mechanisms for such sex-differences are not well understood.

Published
2023
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4. The onset of late severe lung impairment in COVID-19 is associated with high inflammation markers at admission and metabolic syndrome markers

Author

Olivier Epaulard, Audrey Le Gouellec, Marion Le Marechal, Benjamin Nemoz, Anne-Laure Borel, Anaïs Dartevel, Hubert Gheerbrant, Marie-Christine Herault, Annick Bosseray, Giovanna Clavarino, Julien Lupo, Damien Viglino, Fanny Quenard, Clara Candille, Boubou Camara, Michel Durand, Patrice Faure, Dorra Guergour, Elena Chidlovski, Marie-Christine Jacob, Sylvie Larrat, Marie Froidure, Nicolas Terzi, Sébastien Quetant, Jean-François Payen, Barbara Colombe, Tatiana Raskovalova, Patrice Morand, Isabelle Pierre, Carole Schwebel, Rebecca Hamidfar, Laurence Bouillet, Jean-Paul Brion, Candice Trocme, Sylvie Berthier, Carole Chirica, Anne-Laure Mounayar, Myriam Blanc, Patricia Pavese, and Bertrand Toussaint

Abstract

BackgroundCOVID-19 severity is mainly related to lung impairment. However, preexisting patient characteristics and biomarkers at admission associated with this event are not precisely known.MethodsWe report 205 patients admitted for a proven COVID-19 in our institution between March 7 and April 22, 2020, particularly their comorbidities, respiratory severity, immune profile, and metabolic profile.FindingsMedian age was 70 years [interquartile range (IQR) 25-75: 60;79]; 115 (56·1%) patients were men. Oxygen supplementation of >2L/min was required in 107 patients (52·2%) after a median time of 8 days [IQR: 6;10] after the first symptoms; 67 (32·7%) patients were admitted to the intensive care unit (ICU), almost exclusively due to severe hypoxia. Patients requiring >2L/min oxygen therapy and/or ICU admission were older and more frequently males, with a significantly higher body mass index (BMI), a significantly higher total cholesterol (TC) / HDL cholesterol ratio, and higher triglycerides. They also had higher plasma levels of C-reactive protein (CRP) and interleukin 6 (IL-6); IL-6 >20 ng/L and CRP >70 mg/L were significantly associated with ICU admission and/or (for patients with a decision of limitation of life-support therapy) death. Higher BMI and TC/HDL-c ratio were associated with higher CRP and IL-6 levels. Steroid therapy was performed in 61 patients; while its clinical impact was inconclusive due to heterogeneous situations, IL-6 levels decreased significantly more in these patients.InterpretationSevere COVID-19 mostly relates to late-onset pneumonia associated with preexisting metabolic syndrome markers and a surge in inflammatory markers, allowing the early identification of at-risk patients.FundingThis work was supported by Foundation University of Grenoble Alpes.

Published
2022
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5. Phenol and Phthalate Effects on Thyroid Hormone Levels during Pregnancy: Relying on

Author

Dorothy, Nakiwala, Pamela D, Noyes, Patrice, Faure, Benoît, Chovelon, Christelle, Corne, Anne Sophie, Gauchez, Dorra, Guergour, Sarah, Lyon-Caen, Amrit K, Sakhi, Azemira, Sabaredzovic, Cathrine, Thomsen, Isabelle, Pin, Rémy, Slama, and Claire, Philippat

Subjects
Thyroid Hormones, Adverse Outcome Pathways, Phenol, Phenols, Pregnancy, Thyroid Gland, Phthalic Acids, Humans, Female, Iodine
Abstract

Studies characterizing associations between phenols, phthalates and thyroid hormones during pregnancy produce inconsistent results. This divergence may be partly attributable to false positives due to multiple comparison testing of large numbers of chemicals, and measurement error as studies rely on small numbers of biospecimens despite high intra-individual variability in urinary chemical metabolite concentrations.This study employsA two-tiered approach was implemented:The ToxCast/Tox21 screening reduced the chemical set from 16 to 13 and the associated number of statistical comparisons by 19%. Parabens were negatively associated with free triiodothyronine (T3) and the T3/T4 (total thyroxine) ratio. Monobenzyl phthalate was positively associated with total T4 and negatively with the T3/T4 ratio. Effect modification by iodine status was detected for several compounds (among themFor these chemicals, screening for compounds with an increased likelihood for thyroid-related effects and relying on repeated urine samples to assess exposures improved the overall performance of multichemical analyses of thyroid disruption. This approach may improve future evaluations of human data for the thyroid pathway with implication for fetal health and may serve as a model for evaluating other toxicity outcomes. https://doi.org/10.1289/EHP10239.

Published
2022

6. Phenol and Phthalate Effects on Thyroid Hormone Levels during Pregnancy: Relying on In Vitro Assays and Adverse Outcome Pathways to Inform an Epidemiological Analysis

Author

Dorothy Nakiwala, Pamela D. Noyes, Patrice Faure, Benoît Chovelon, Christelle Corne, Anne Sophie Gauchez, Dorra Guergour, Sarah Lyon-Caen, Amrit K. Sakhi, Azemira Sabaredzovic, Cathrine Thomsen, Isabelle Pin, Rémy Slama, and Claire Philippat

Subjects
Health, Toxicology and Mutagenesis, Public Health, Environmental and Occupational Health
Published
2022

7. Author response for 'Influenza vaccination and prognosis of COVID ‐19 in hospitalized patients with diabetes: Results from the CORONADO study'

Author

null A Diallo, null M Pichelin, null M Wargny, null P Gourdy, null JB Bonnet, null S Hadjadj, null B Cariou, null A Sultan, null F Galtier, null Matthieu Wargny, null Pascale Mahot, null Bertrand Cariou, null Samy Hadjadj, null Matthieu Pichelin, null Anne‐Laure Fournier‐Guilloux, null Nicolas Mauduit, null Edith Bigot‐Corbel, null Anne‐Sophie Boureau, null Laure Dekcer, null Audrey Ernould, null Claire Primot, null Anne Seguin, null Marielle Joliveau, null Sonia Pouvreau, null Chloé FOURNIER, null Jeremy Thureau, null Edith Fonteneau, null Pamela Hublain, null Chu Nantes, null Carole Agasse, null Mathilde DE Kergaradec, null Vincent Minville, null Fanny Vardon‐Bounes, null Guillaume Martin‐Blondel, null Pierre Gourdy, null Blandine Tramunt, null Marie‐Christine Turnin, null Hélène Hanaire, null Jean‐Michel Mansuy, null Didier Fabre, null Marie‐Blanche Arhainx, null Laurent Cazals, null Laure Combes, null Emmanuelle Lami, null Mallory Cianferani, null Bruno Megarbane, null Pierre Leroy, null Jean‐François Gautier, null Tiphaine Vidal‐Trecan, null Jean‐Pierre Riveline, null Jean‐Louis Laplanche, null Stéphane Mouly, null Louis Potier, null Ronan Roussel, null Malak Taher, null Yawa Abouleka, null Fetta Yaker, null Aurelie Carlier, null Anne Boutten, null Marilyne Hallot‐Feron, null Fadila Mourah, null Charles Thivolet, null Emilie Blond, null Muriel Rolland, null Josep Verdecho Mendez, null Marine Alexandre, null Julien Pottecher, null Emilie Richer, null Laurent Meyer, null Florina Luca, null Jean‐Marc Lessinger, null Thibault Bahougne, null Bruno Guerci, null Lisa Ludwig, null Siham Benzirar, null Catherine Malaplate, null Thierry Matton, null Julien Poissy, null Karine Faure, null Pierre Fontaine, null Florence Baudoux, null Anne Vambergue, null Jean David Pekar, null Marc Lambert, null Cécile Yelnik, null Amélie Bruandet, null Laurent Petit, null Didier Neau, null Vincent Rigalleau, null Annie Berard, null Amandine Galioot, null Remy Coudroy, null Arnaud Thille, null René Robert, null France Roblot‐Cazenave, null Blandine Rammaert, null Pierre Jean Saulnier, null Xavier Piguel, null Nesrine Benhenda, null Camille Husson, null Celine Olivier, null Florence Torremocha, null Mathilde Fraty, null Marie Flamen d'assigny, null Aurelie Miot, null Valentin Bossard, null Kada Klouche, null Alain Makinson, null Ariane Sultan, null Jean‐Baptiste Bonnet, null Vincent Foulongne, null Florence Galtier, null Cécile Aubron, null Séverine Ansart, null Véronique Kerlan, null Pascale Quiniou, null Jean‐Luc Carre, null Stéphane Quesnot, null Bruno Laviolle, null Carole Schwebel, null Olivier Epaulard, null Pierre‐Yves Benhamou, null Cécile Betry, null Anne‐Laure Borel, null Sandrine Lablanche, null Dorra Guergour, null Catherine Duclos, null Emmanuel Cosson, null Erwan Guyot, null Aurore Deniau, null Phucthutrang Nguyen, null Yves Reznik, null Michael Joubert, null Stéphane Allouche, null Lydia Guittet, null Steven Grange, null Manuel Etienne, null Gaëtan Prévost, null Valéry Brunel, null Jean‐Christophe Lagier, null Didier Raoult, null Anne Dutour, null Bénédicte Gaborit, null Sandrine Boulllu, null Patrice Darmon, null Adèle Lasbleiz, null Mathieu Cerino, null Fanny Romain, null Marie Houssays, null Jean Pierre Quenot, null Lionel Piroth, null Bruno Vergès, null Laurence Duvillard, null Bernard Bonnotte, null Alain Mercat, null Vincent Dubee, null Ingrid Allix, null Patrice Rodien, null Robin Dhersin, null Maylis Lebeault, null Wojciech Trzepizur, null Jocelyne Loison, null Antoine Brangier, null Pierre Asfar, null Pascal Reynier, null Françoise Larcher, null Françoise Joubaud, null Marie‐Rita Andreu, null Geoffrey Urbanski, null Laurent Hubert, null Cedric Annweiler, null Jean Dellamonica, null Johan Courjon, null Nicolas Chevalier, null Giulia Chinetti, null Magda Chafai, null Bruno Mourvillier, null Firouze Bani‐Sadr, null Sarra Barraud, null Brigitte Delemer, null Philippe Gillery, null Pascale Labedade, null Amélie Chabrol, null Alfred Penfornis, null Catherine Petit, null Coralie Amadou, null Maxime Adler, null Clément Dubost, null Pierre‐Louis Conan, null Lyse Bordier, null Franck Ceppa, null Cyril Garcia, null Mathilde Sollier, null Olivier Dupuy, null Sophie Laplance, null Olivier Billuart, null Marie Joseph Aroulanda, null Frédérique Olivier, null Florence Ayon, null Nathalie Wilhelm, null Loic Epelboin, null Nadia Sabbah, null Aurelie Charpin, null Pierre Squara, null Olivier Belliard, null Claude Dubois, null Michel Marre, null Johann Auchabie, null Roxane Courtois, null Thierry Duriez, null Tiphaine Mergey, null Laura Vallee, null Laetitia Seguin, null Abdallah Al‐Salameh, null Jean‐Philippe Lanoix, null Sandrine Soriot‐Thomas, null Anne‐Marie Bourgeois‐Descouls, null Rachel Desailloud, null Natacha Germain, null Bogdan Galusca, null Gwenaelle Belleton, null Nesrine Marouani, null Delia Palaghiu, null Amira Hammour, null Fernando Berdaguer, null Thimothée Klopfenstein, null Hajer Zayet, null Patrice Winiszewski, null Marie Zanusso, null Pauline Garnier, null Ingrid Julier, null Karim Hamzaoui, null Sophie Marty‐Gres, null Tarik Sadki, null Lucile Cadot, null Jean‐Louis Dubost, null Céline Gonfroy, null Catherine Campinos, null Pascale Martres, null Marie Pierre Coulhon, null Nicolas Allou, null Marwa Bachir, null Stella Hoang, null Candice Kembellec, null Olivia Suply, null Fatima Kharcha, null Anne‐Claire Devouge, null Anna Flaus‐Furmanuk, null Isabelle Madeline, null Vincent Ehinger, null Sophie Bastard, null Loic Raffray, null Frederic Renou, null Aude Bojarski, null Caroline Paul, null Karine Borsu, null Angelique Gorlin, null Servane Bernardo, null Carole Truong Ut, null Stephane Renaud, null Antoine Vignoles, null Emilie Foch, null Laurie Masse, null Hubert Grand, null Helene Ferrand, null Christelle Raffaitin‐Cardin, null Hadjer Zellagui, null Celine Castang‐Brachet, null Frederique Boury, null Ana Alvarez Tena, null Isabelle Moura, null Pierre Kalfon, null Juliana Darasteanu, null Arnaud Monier, null Pascal Foucault, null Alexandra Depuille, null Stéphanie Laugier‐Robiolle, null Patrick Caneiro, null Maud Basso, null Etienne Larger, null Samir Bouam, null Wahiba Benzenati, null Leila Ait Bachir, null Camille Cussac Pillegand, null Marc Vasse, null Christophe Michard, null Nathanaëlle Montanier, null Luc Millot, null Françoise Crepet, null Danielle Ratsimba, null Kevin Bouiller, null Sophie Borot, null Isabelle Bruckert, null Annie Clergeot, null Franck Schillo, null Dorothée Vignes, null Muriel Bourgeon‐Ghittori, null Hamoud Lachgar, null Claire Lambert DE Cursay, null Stéphane Levante, null Jean Charles Auregan, null Antoine Merlet, null Cécile Zaragoza, null Gwénaëlle Arnault, null Anne‐Gaëlle Loupp, null Olivier Lesieur, null Mariam Roncato‐Saberan, null Didier Gouet, null Romain Lemarie, null Hong_an Allano, null Emmanuel Vivier, null Caroline Pariset, null Cédric Luyton, null Lucien Marchand, null Fanny Doroszewski, null Matthieu Pecquet, null Laurent Perard, null Sylvie Vuillermoz‐Blas, null Nicolas Kacki, null Patricia Charrier, null Amélie Ducet‐Boiffard, null Françoise Desroys Roure, null Olivier Bourron, null Dominique Bonnefont‐Rousselot, null Suzanne Laroche, null Franck Phan, null Agnès Hartemann, null Cyrielle Caussy, null Emmanuel Disse, null Claude Guerin, null Thomas Perpoint, null Philippe Moulin, null Régine Cartier, null Geoffroy Hariri, null Dorothée Chopin, null Camille Vatier, null Nathalie Bourcigaux, null Emmanuelle Chaigneau, null Sophie Christin‐Maitre, null Bruno Donadille, null Bruno Feve, null Sophie Lamothe, null Julie Sarfati, null Pascal Pernet, null Anne Chambon, null Delphine Demarsy, null Hugo Campagne, null Françoise Latil‐Plat, null Monica Berne, null Marilyne Grinand, null Marion Touzet, null Aydrey Zabulon, null Jocelyne Craspag, null Catherine Ledoux, null Cedric Contaret, null Blandine Janand‐Delenne, null Anaïs Giraud, null Marie Lou Lacrimini, null Joëlle Arrivie, null Deborah Ancelle, null Carine Guillois, null Bénédicte Fremy, null Amina Chaalal, null Gaëlle Barrande, null Anne Dorange, null Eglantine Rouanet, null Dominique Seret‐Begue, null Audrey Saoud, null Anne‐Marie Guedj, null Nathalie Bedos, null Fritz‐Line Velayoudom, null Marie Dumas, null Benoite Gonda, null Christine Coffin, null Stéphanie Gibiat, null Myriam Lungo, null Chantal Bully, null Pierre Serusclat, null Stella Bully, null Patricia Carre, null Jean‐Philippe Leberre, null Carlos Elkhoury, null Marine Thieux, null Laetitia Paradisi‐Prieur, and null CORONADO investigators

Subjects
Vaccination, Pediatrics, medicine.medical_specialty, Coronavirus disease 2019 (COVID-19), business.industry, Hospitalized patients, Diabetes mellitus, Medicine, business, medicine.disease
Published
2021
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8. Phenols, phthalates and thyroid hormone levels during pregnancy; relying on toxicological data and Adverse Outcome Pathways to inform epidemiological analysis

Author

Christelle Corne, Isabelle Pin, Anne Sophie Gauchez, Cathrine Thomsen, Sarah Lyon Caen, Pamela D. Noyes, Azemira Sabaredzovic, Dorothy Nakiwala, Rémy Slama, Patrice Faure, Benoit Chovelon, Claire Philippat, Amrit Kaur Sakhi, and Dorra Guergour

Subjects
Pregnancy, medicine.medical_specialty, business.industry, Thyroid, Bioinformatics, medicine.disease, medicine.anatomical_structure, Adverse Outcome Pathway, Epidemiology, General Earth and Planetary Sciences, Medicine, business, General Environmental Science, Hormone
Published
2021
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9. Estimating sodium intake from spot urine samples at population level: a validation and application study in French adults

Author

Charlotte Verdot, Katia Castetbon, Serge Hercberg, Pilar Galan, Valérie Deschamps, Michel Vernay, Camille Lassale, Dorra Guergour, Benoît Salanave, Emmanuelle Kesse-Guyot, Patrice Faure, Elise Emeville, Observatoire Régional de la Santé de la Guadeloupe, Department of Epidemiology and Public Health, University College of London [London] (UCL), IMIM-Hospital del Mar, Generalitat de Catalunya, Ecole de Santé Publique [Université Libre de Bruxelles], Université libre de Bruxelles (ULB), Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle (CRESS - U1153), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Santé publique France - French National Public Health Agency [Saint-Maurice, France], Centre Hospitalier Universitaire [Grenoble] (CHU), Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Equipe 1 : EPOPé - Épidémiologie Obstétricale, Périnatale et Pédiatrique (CRESS - U1153), Université Paris Descartes - Paris 5 (UPD5)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), French Ministry of Health, Sante publique France, Fondation pour la Recherche Medicale, Institut National de la Sante et de la Recherche Medicale (Inserm), Institut National de la Recherche Agronomique (INRA), Conservatoire National des Arts et Metiers (CNAM), University of Paris 13, Region Ile-de-France, Caisse nationale d'Assurance maladie des Travailleurs salaries (CnamTS), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), and AUDOUX, Michèle

Subjects
Adult, Male, Time Factors, urinary sodium excretion, Population level, [SDV]Life Sciences [q-bio], Population, Medicine (miscellaneous), Urine, 030204 cardiovascular system & hematology, Santé publique, Spearman's rank correlation coefficient, Urine collection device, Excretion, 03 medical and health sciences, 0302 clinical medicine, Animal science, Humans, 030212 general & internal medicine, Salt intake, education, False Negative Reactions, Aged, Urine Specimen Collection, Mathematics, education.field_of_study, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Nutrition and Dietetics, Sodium, spot urine samples, Sodium, Dietary, predictive intersalt equation, Middle Aged, Epidémiologie, salt intake, Nutrition Surveys, Diet Records, Diet, 3. Good health, [SDV] Life Sciences [q-bio], Spot urine, Mental Recall, predictive INTERSALT equation, Female, France, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

The aim of this study was to assess the validity of the predictive INTERSALT equation using spot urine samples to estimate 24-hour urinary sodium (24-hUNa) excretion and daily sodium intake among the French adult population. Among 193 French adults ("validation sample"), we assessed the validity by comparing predicted 24-hUNa excretion from spot urine and measured 24-hUNa excretion from 24-hour urine collections. Spearman correlation coefficients and Bland-Altman plots were used and we calculated calibration coefficients. In a nationally representative sample of 1,720 French adults ("application sample"), the calibrated predictive equation was then applied to the spot urine Na values to estimate 24-hUNa excretion and daily Na intake. In that sample, predicted Na intake was compared to that estimated from 24-hour dietary recalls. Results were adjusted and corrected using calibration coefficients. In the validation sample, the measured 24-hUNa excretion was on average 14% higher than the predicted 24-hUNa (+ 13% for men and + 16% for women). Correlation between measured and predicted 24-hUNa excretion was moderate (Spearman r = 0.42) and the Bland-Altman plots showed underestimation at lower excretion level and overestimation at higher level. In the application study, estimated daily salt intake was 8.0 g/day using dietary recalls, 8.1 g/day using predicted INTERSALT equation and 9.3 g/day after applying calibration coefficients calculated in the validation study. Despite overall underestimation of 24-hUNa excretion by spot urinary sodium, the use of predictive INTERSALT equation remains an acceptable alternative in monitoring global Na intake/excreted in the French population but its use is not advised at the individual level., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2019
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10. Cardiopulmonary responses during the cooling and the extracorporeal life support rewarming phases in a porcine model of accidental deep hypothermic cardiac arrest

Author

Bertrand Perrin, Angélique Brouta, Maxime Maignan, Candice Trocme, Guillaume Debaty, Vincent Bach, Stéphane Tanguy, Raphaël Briot, Dorra Guergour, Physiologie cardio-Respiratoire Expérimentale Théorique et Appliquée (TIMC-IMAG-PRETA), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire d'Etude et Modélisation des Environnements littoraux (LEMEL), Université de Bretagne Sud (UBS), Contrôle moléculaire de la réponse immune specifique, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Groupe de Recherche et d'Etude du Processus Inflammatoire (GREPI), Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Clinique de chirurgie cardiaque, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])

Subjects
Cardiac output, endocrine system, Swine, medicine.medical_treatment, Resuscitation, Hemodynamics, 030204 cardiovascular system & hematology, Doppler echocardiography, Critical Care and Intensive Care Medicine, Extracorporeal life support, Body Temperature, 03 medical and health sciences, 0302 clinical medicine, Extracorporeal Membrane Oxygenation, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Hypothermia, Induced, medicine.artery, Extracorporeal membrane oxygenation, Medicine, Animals, Rewarming, ComputingMilieux_MISCELLANEOUS, Original Research, medicine.diagnostic_test, business.industry, 030208 emergency & critical care medicine, Hypothermia, Cardiac arrest, Heart Arrest, Disease Models, Animal, medicine.anatomical_structure, Blood pressure, Anesthesia, Deep hypothermic cardiac arrest, Pulmonary artery, Vascular resistance, Emergency Medicine, Female, medicine.symptom, business, Accidental hypothermia
Abstract

Background This study aimed to assess cardiac and pulmonary pathophysiological responses during cooling and extracorporeal life support (ECLS) rewarming in a porcine model of deep hypothermic cardiac arrest (DHCA). In addition, we evaluated whether providing a lower flow rate of ECLS during the rewarming phase might attenuate cardiopulmonary injuries. Methods Twenty pigs were cannulated for ECLS, cooled until DHCA occurred and subjected to 30 min of cardiac arrest. In order to assess the physiological impact of ECLS on cardiac output we measured flow in the pulmonary artery using Doppler echocardiography as well as a modified thermodilution technique using the Swan-Ganz catheter (injection site in the right ventricle). The animals were randomized into two groups during rewarming: a group with a low blood flow rate of 1.5 L/min (LF group) and a group with a normal flow rate of 3.0 L/min (NF group). The ECLS temperature was adjusted to 5 °C above the central core. Cardiac output, hemodynamics and pulmonary function parameters were evaluated. Results During the cooling phase, cardiac output, heart rhythm and blood pressure decreased continuously. Pulmonary artery pressure tended to increase at 32 °C compared to the initial value (20.2 ± 1.7 mmHg vs. 29.1 ± 5.6 mmHg, p = 0.09). During rewarming, arterial blood pressure was higher in the NF than in the LF group at 20° and 25 °C (p = 0.003 and 0.05, respectively). After rewarming to 35 °C, cardiac output was 3.9 ± 0.5 L/min in the NF group vs. 2.7 ± 0.5 L/min in LF group (p = 0.06). At the end of rewarming under ECLS cardiac output was inversely proportional to the ECLS flow rate. Moreover, the ECLS flow rate did not significantly change pulmonary vascular resistance. Discussion Using a newly developed experimental model of DHCA treated by ECLS, we assessed the cardiac and pulmonary pathophysiological response during the cooling phase and the ECLS rewarming phase. Despite lower metabolic need during hypothermia, a low ECLS blood flow rate during rewarming did not improved cardiopulmonary injuries after rewarming. Conclusion A low ECLS flow rate during the rewarming phase did not attenuate pulmonary lesions, increased blood lactate level and tended to decrease cardiac output after rewarming. A normal ECLS flow rate did not increase pulmonary vascular resistance compared to a low flow rate. This experimental model on pigs contributes a number of pathophysiological findings relevant to the rewarming strategy for patients who have undergone accidental DHCA.

Published
2016
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11. Discrepancies between the Cockcroft-Gault and Chronic Kidney Disease Epidemiology (CKD-EPI) Equations: Implications for Refining Drug Dosage Adjustment Strategies

Author

Pierre Delanaye, Fabrice Guerber, Hans Pottel, Dorra Guergour, André Scheen, Antoine Bouquegneau, Christophe Mariat, and Timothy Ellam

Subjects
Adult, Male, medicine.medical_specialty, Dose, Concordance, Urology, Renal function, 030204 cardiovascular system & hematology, Models, Biological, Cohort Studies, 03 medical and health sciences, chemistry.chemical_compound, Young Adult, 0302 clinical medicine, Epidemiology, medicine, Humans, Pharmacology (medical), 030212 general & internal medicine, Renal Insufficiency, Chronic, Intensive care medicine, Aged, Pharmacology, Body surface area, Aged, 80 and over, Creatinine, Dose-Response Relationship, Drug, business.industry, Middle Aged, medicine.disease, chemistry, Pharmaceutical Preparations, Female, business, Cohort study, Kidney disease, Glomerular Filtration Rate
Abstract

The dosages of many medications require adjustment for renal function. There is debate regarding which equation, the Chronic Kidney Disease Epidemiology (CKD-EPI) equation vs. the Cockcroft–Gault (CG) equation, should be recommended to estimate glomerular filtration rate. We used a mathematical simulation to determine how patient characteristics influence discrepancies between equations and analyzed clinical data to demonstrate the frequency of such discrepancies in clinical practice. In the simulation, the modifiable variables were sex, age, serum creatinine, and weight. We considered estimated glomerular filtration rate results in mL/min, deindexed for body surface area, because absolute excretory function (rather than per 1.73 m2 body surface area) determines the rate of filtration of a drug at a given plasma concentration. An absolute and relative difference of maximum (±) 10 mL/min and 10 %, respectively, were considered concordant. Clinical data for patients aged over 60 years (n = 9091) were available from one hospital and 25 private laboratories. In the simulation, differences between the two equations were found to be influenced by each variable but age and weight had the biggest effect. Clinical sample data demonstrated concordance between CKD-EPI and CG results in 4080 patients (45 %). The majority of discordant results reflected a CG result lower than the CKD-EPI equation. With aging, the CG result became progressively lower than the CKD-EPI result. When weight increased, the opposite occurred. The choice of equation for excretory function adjustment of drug dosage will have different implications for patients of different ages and body habitus. The optimum equation for drug dosage adjustment should be defined with consideration of individual patient characteristics.

Published
2016

12. Post-conditioning with Cyclosporine A after a 24-hour cold ischemia in ex vivo reperfused pig lungs

Author

Marie-Hélène Schmidt, Maxime Maignan, Stéphane Gennai, Raphaël Briot, Dorra Guergour, Physiologie cardio-Respiratoire Expérimentale Théorique et Appliquée (TIMC-IMAG-PRETA), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Contrôle moléculaire de la réponse immune specifique, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF)

Subjects
Pulmonary and Respiratory Medicine, Time Factors, Swine, medicine.medical_treatment, Clinical Biochemistry, Ischemia, Post conditioning, 030204 cardiovascular system & hematology, 03 medical and health sciences, 0302 clinical medicine, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, medicine, Animals, Cold ischemia, Receptor, Ischemic Postconditioning, Molecular Biology, Lung, ComputingMilieux_MISCELLANEOUS, Mechanical ventilation, business.industry, Cold Ischemia, medicine.disease, Advanced Glycation Endproducts, 3. Good health, medicine.anatomical_structure, 030228 respiratory system, Anesthesia, Reperfusion Injury, Cyclosporine, business, Ex vivo
Abstract

To evaluate the effects of 1 and 5 μM of Cyclosporine A (CsA), administered 24 hours after a cold ischemic period, in an ex vivo reperfused pig lung model.The experiments were performed in 15 pigs. Each pair of lungs was surgically separated. Extracorporeal perfusion and mechanical ventilation were started after a cold ischemia of 2 hours for one lung and 24 hours for the contralateral. We constituted three groups (n = 5 each): two groups for which the lung underwent a 24-hour ischemia received either 1 or 5 μM of CsA at the time of reperfusion, and a control group without CsA. For each group, lungs undergoing a 2-hour ischemia did not receive CsA.Reperfusion with either CsA increased the PO2 levels in a dose dependent manner, and reduced concentrations of the receptor for advanced glycation endproducts, compared to the control. The pulmonary arterial pressure, the capillary pressure, and the pulmonary vascular resistances were not increased, even with 5 μM of CsA. No significant change was shown on cytokines levels.Postconditioning with CsA improves lung function, after a 24-hour cold ischemic period. Either 1 or 5 μM seemed to be safe regarding the pulmonary vascular pressures and resistances.

Published
2015
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13. Impact of intra-arrest therapeutic hypothermia in outcomes of prehospital cardiac arrest: a randomized controlled trial

Author

Vincent Danel, Jean François Payen, Françoise Carpentier, Hélène Bouvaist, Stéphane Ruckly, Christophe Escallier, Pierre Yves Gueugniaud, Clémence Minet, Dominique Savary, Charles Santre, Christophe Adrie, Julien Picard, Loic Belle, Laure Hammer, Maxime Maignan, Renaud Chouquer, Guillaume Debaty, Jean-François Timsit, François Xavier Koch, Dorra Guergour, Michel Durand, Physiologie cardio-Respiratoire Expérimentale Théorique et Appliquée (TIMC-IMAG-PRETA), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Centre Hospitalier Universitaire [Grenoble] (CHU), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Centre Hospitalier Annecy-Genevois [Saint-Julien-en-Genevois], Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC), Clinique de réanimation médicale, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-Hôpital Michallon, Service d'anesthésie-réanimation, COIITSS Study Investigators, Centre hospitalier d'Annecy, Unité de pneumologie, CHU Grenoble, Contrôle moléculaire de la réponse immune specifique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF), Service de Cardiologie, Centre hospitalier de la région d'Annecy, Service de réanimation chirurgicale [Béclère], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Antoine Béclère [Clamart], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'accueil des urgences médicales, Société Française de Médecine d'Urgence [Paris, France], Samu 38, Institut Albert Bonniot, Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Resuscitation, medicine.medical_specialty, business.industry, medicine.medical_treatment, Hypothermia, Return of spontaneous circulation, Critical Care and Intensive Care Medicine, 3. Good health, law.invention, Randomized controlled trial, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, law, Anesthesia, Anesthesiology, Clinical endpoint, Medicine, Cardiopulmonary resuscitation, medicine.symptom, business, Clinical death, ComputingMilieux_MISCELLANEOUS
Abstract

Mild therapeutic hypothermia (TH) is recommended as soon as possible after the return of spontaneous circulation to improve outcomes after out-of-hospital cardiac arrest (OHCA). Preclinical data suggest that the benefit of TH could be increased if treatment is started during cardiac arrest. We aimed to study the impact of intra-arrest therapeutic hypothermia (IATH) on neurological injury and inflammation following OHCA. We conducted a 1:1 randomized, multicenter study in three prehospital emergency medical services and four critical care units in France. OHCA patients, irrespective of the initial rhythm, received either an infusion of cold saline and external cooling during cardiac arrest (IATH group) or TH started after hospital admission (hospital-cooling group). The primary endpoint was neuron-specific enolase (NSE) serum concentrations at 24 h. Secondary endpoints included IL-6, IL-8, and IL-10 concentrations, and clinical outcome. Of the 245 patients included, 123 were analyzed in the IATH group and 122 in the hospital-cooling group. IATH decreased time to reach temperature ≤34 °C by 75 min (95 % CI: 4; 269). The rate of patients admitted alive to hospital was not different between groups [IATH n = 41 (33 %) vs. hospital cooling n = 36 (30 %); p = 0.51]. Levels of NSE and inflammatory biomarkers were not different between groups [median NSE at 24 h: IATH 96.7 μg/l (IQR: 49.9–142.8) vs. hospital cooling 97.6 μg/l (IQR: 74.3–142.4), p = 0.64]. No difference in survival and cerebral performance were found at 1 month. IATH did not affect biological markers of inflammation or brain damage or clinical outcome.

Published
2014
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14. Determination of anti–Toxoplasma gondii immunoglobulin G avidity: adaptation to the vidas system (bioMérieux)

Author

Andrée Goullier-Fleuret, Hervé Pelloux, Michel Jolivet, Suzanne Marcillat, Dorra Guergour, Guy Vernet, Emmanuelle Brun, Pierre Ambroise-Thomas, and Hélène Fricker-Hidalgo

Subjects
Microbiology (medical), Cellular immunity, Antibody Affinity, Antibodies, Protozoan, Immunoglobulin G, Serology, Immunoenzyme Techniques, Pregnancy, parasitic diseases, medicine, Animals, Humans, Avidity, Seroconversion, biology, Toxoplasma gondii, General Medicine, medicine.disease, biology.organism_classification, Virology, Toxoplasmosis, Infectious Diseases, Pregnancy Complications, Parasitic, Immunology, biology.protein, Female, Reagent Kits, Diagnostic, Antibody, Toxoplasma
Abstract

The determination of specific anti-Toxoplasma gondii IgG avidity has been proposed to improve the determination of the date of toxoplasmic seroconversion in pregnant women. In this study, we adapted this serological technique to the Vidas system (bioMérieux) using 6 M urea as the dissociating agent. We studied 356 sera, including 42 sequential sera from sero-conversions in pregnant women. Our results show that the test is easy to use, and that an avidity index higher than 0.300 allows the exclusion of a recent infection acquired less than 4 months before serum sampling.

Published
1998
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15. Evaluation of LOCI technology-based thyroid blood tests on the Dimension Vista analyzer

Author

S. Vergnaud, A.S. Gauchez, François Laporte, Denis Monneret, Patrice Faure, and Dorra Guergour

Subjects
endocrine system, medicine.medical_specialty, Thyroid Hormones, endocrine system diseases, Clinical Biochemistry, Serum albumin, Thyroid Gland, Thyrotropin, Internal medicine, medicine, Humans, In patient, Immunoassay, Hematologic Tests, medicine.diagnostic_test, biology, Chemistry, Thyroid, Albumin, Radioimmunoassay, General Medicine, Thyroxine, Concordance correlation coefficient, medicine.anatomical_structure, Endocrinology, Luminescent Measurements, biology.protein, Triiodothyronine, hormones, hormone substitutes, and hormone antagonists, Hormone
Abstract

Objectives To assess the performance of thyroid-stimulating hormone (TSH), free thyroxine (FT4) and free triiodothyronine (FT3) determinations by luminescent oxygen channeling immunoassay (LOCI®) technology on the Dimension Vista® analyzer (Siemens Healthcare Diagnostics). Design & methods We assessed 1) functional sensitivity for TSH (FSe-TSH), and intra- and inter-assay variations for TSH, FT4 and FT3 on Vista®; 2) comparisons of serum and heparin-treated plasma on Vista®; 3) comparisons of a) plasma TSH by Vista® versus electrochemiluminescence (ECLIA) on Roche Modular® analyzer, and b) plasma FT4 and FT3 by Vista® versus Immunotech®-Beckman radioimmunoassay (RIA); and 4) association of albumin and prealbumin levels with free thyroid hormone concentrations on Vista®. Results 1) FSe-TSH concentration was below 0.005 mIU/L. Maximum intra-assay CVs (2.1%, 1.4%, 5.2%) and inter-assay CVs (16.5%, 5.1%, 5.8%) were good for TSH, FT4 and FT3 respectively. 2) Heparin-treated plasma samples consistently gave slightly higher values than serum for TSH, FT4 and FT3. 3) Passing–Bablok regression gave: TSH: [LOCI] = 0.91[ECLIA] − 0.08 (concordance correlation coefficient ρ c = 0.95); FT4: [LOCI] = 1.05[RIA] − 1.55 ( ρ c = 0.80); and FT3: [LOCI] = 1.05[RIA] − 0.06 ( ρ c = 0.81). 4) Both serum albumin and prealbumin concentrations were positively associated with FT3 levels and negatively associated with FT4 levels in patients. Conclusion LOCI® is accurate for TSH, FT4 and FT3 analysis. Despite a slight significant bias compared to ECLIA, LOCI® is precise for TSH and fulfills the third-generation criteria. However, the poor concordance between LOCI® and RIA for FT4 and FT3, and the dependence of these hormones on binding proteins require further investigation.

Published
2012

16. Electronic sorting and recovery of single live cells from microlitre sized samples

Author

Luigi Altomare, Christian L. Villiers, Mélanie Abonnenc, Francois Chatelain, Patrice N. Marche, D. Freida, Alexandra Fuchs, Roberto Guerrieri, Gianni Medoro, Dorra Guergour, Aldo Romani, Isabelle Chartier, Marco Tartagni, Nicolò Manaresi, Marche, Patrice, Laboratoire Biopuces (BIOPUCES), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Advanced Research Center on Electronic Systems for Information and Communication Technologies E. De Castro [Bologna] (ARCES), Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Silicon Biosystems, Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Contrôle moléculaire de la réponse immune specifique, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), This project was funded by the European Commission (MeDICS project, EU grant IST-2001-32437), and is supported by Italian Ministry of Research under FIRB project., A.B. Fuch, A. Romani, D. Freida, G. Medoro, M. Abonnenc, L. Altomare, I. Chartier, D. Guergour, C. Villier, P.N. Marche, M. Tartagni, R. Guerrieri, F. Chatelain, and N. Manaresi

Subjects
Thousand cells, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Cell Survival, Population, Biomedical Engineering, Bioengineering, Nanotechnology, Cell Separation, 02 engineering and technology, Biology, 01 natural sciences, Biochemistry, MESH: Cell Separation, Electrophoresis, Microchip, Cell therapy, MESH: Cell Proliferation, MESH: Electrophoresis, Microchip, education, cell sorting, MESH: Sample Size, Cell Proliferation, dielectrophoresis, education.field_of_study, Cell growth, 010401 analytical chemistry, Sorting, General Chemistry, 021001 nanoscience & nanotechnology, Chip, 0104 chemical sciences, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, MESH: Cell Survival, Sample Size, Stem cell, 0210 nano-technology, cell chip, Cell based
Abstract

International audience; Sorting and recovering specific live cells from samples containing less than a few thousand cells have become major hurdles in rare cell exploration such as stem cell research, cell therapy and cell based diagnostics. We describe here a new technology based on a microelectronic chip integrating an array of over 100,000 independent electrodes and sensors which allow individual and parallel single cell manipulation of up to 10,000 cells while maintaining viability and proliferation capabilities. Manipulation is carried out using dynamic dielectrophoretic traps controlled by an electronic interface. We also demonstrate the capabilities of the chip by sorting and recovering individual live fluorescent cells from an unlabeled population.

Published
2006
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18. Élaboration et mise en œuvre des vérifications de méthodes lors d’un renouvellement d’automates : exemple de l’unité de biochimie immunoanalyse du CHU Grenoble Alpes

Author

Despinasse, Quentin, Université Grenoble Alpes - UFR Pharmacie (UGA UFRP), Université Grenoble Alpes (UGA), and Dorra Guergour

Subjects
Norme ISO 15189, Accréditation, Vérification de méthodes, Immunoanalyse, Chimie, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, Atellica®, Renouvellement d’automates
Abstract

Mémoire de Diplôme d'Etudes Spécialisées (DES) tenant lieu de thèse d'exercice.; Le renouvellement d’une installation d’automates à flux élevé est un processus long et complexe nécessitant la mise en place d’une gestion de projet globale : il est en effet indispensable de respecter à la fois des contraintes temporelles et de continuité d’activité, de ressources financières et humaines, ainsi que l’obligation d’accréditation à laquelle sont soumis les laboratoires de biologie médicale (norme ISO 15189). La réalisation des dossiers de vérification de méthode, devant être finalisée avant la mise en production avec les nouveaux systèmes, est de ce fait un point critique à impérativement maîtriser. Il a été choisi dans ce travail de se concentrer sur la gestion de quelques points clés de ce sous-processus par l’Unité de Biochimie Immunoanalyse du CHU de Grenoble Alpes, qui a géré lors du renouvellement d’équipements survenu en 2019 la transition de 3 Vista® et un Centaur® (Siemens) à 2 lignes Atellica Solution® (Siemens). En effet, si le processus de vérification de méthodes est globalement bien maîtrisé par les laboratoires, certains éléments méritent d’être discutés, la spécificité inhérente au grand nombre d’analytes concernés par le changement d’automates de chimie/immunoanalyse ajoutant une contrainte non négligeable. Ainsi, les différentes méthodes de détermination des limites acceptables relatives aux essais de répétabilité et de reproductibilité ont été décrites. De la même façon, les problématiques de mise en place (choix des échantillons utilisés, organisation, méthode, objectifs) ainsi que plusieurs pistes d’interprétation des résultats des essais de comparaison de méthode sont détaillées. Découlant en partie de cette interprétation, une discussion sur l’éventuelle modification des intervalles de référence (méthodes de détermination ou de vérification) est également abordée. Une autre partie de ce travail est consacrée à la gestion des interférences, tout particulièrement de celles liées à l’hémolyse. Une démarche en 3 axes y est décrite : changement d’échelle d’indice du nouvel automate, analyse des biais annoncés par le fournisseur sur Atellica®, et modification des conduites à tenir face à une interférence avérée lorsque c’est nécessaire. Pour chacun de ces points, les exigences normatives, problématiques et écueils éventuels sont développés, puis le choix du laboratoire parmi différentes attitudes possibles est argumenté. Cette thèse, s’appuyant sur l’expérience du renouvellement d’automates de l’Unité Biochimie Immunoanalyse du CHU de Grenoble Alpes, fournit ainsi une base de travail ainsi que diverses ressources (logigrammes, tableaux) sur des points critiques de la vérification de méthodes, permettant une mise en conformité avec la norme ISO 15189 et la réussite de la mise en production.

Published
2020

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