5 results on '"Doryan Grivault"'
Search Results
2. Male pig pubertal development and risk of boar taint
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Claire Dugué, Stéphane Ferchaud, Doryan Grivault, Mercat, M. J., Boulot, S., Armelle Prunier, Catherine Larzul, Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Expérimentale Elevages Porcins Innovants (GenESI), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut du Porc (IFIP), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), EAAP, and Bernard, Emilie
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endocrine system ,urogenital system ,[SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies ,[SDV.SA.SPA] Life Sciences [q-bio]/Agricultural sciences/Animal production studies - Abstract
International audience; Male pigs are commonly surgically castrated, without anaesthesia, to avoid a meat quality defect called boar taint, partially due to accumulation of androstenone in fat. Androstenone is a steroid produced by the testes. To decrease boar taint without using castration, a selection against androstenone should be considered. The purpose of this study is to estimate the relationships between androstenone and reproductive traits in boars. In this study we collected 34 traits for 36 Duroc boars, 40 French Landrace boars and 38 Pietrain Axiom NN boars. Characteristics of the semen (number of spermatozoids, mobility, motility, volume, concentration, duration of ejaculation and viability), libido indicators (number of training sessions before the first successful collection and percentage of later refusals), plasma testosterone and oestradiol levels, backfat levels of androstenone, skatole and indole at 180 and 280 day old, the age at 100 kg, the weight of genital tract and indicators of animal behaviour, health and welfare (human-animal relationship test, tears, health and leg scores). We estimated a high Pearson correlation between plasma oestradiol and androstenone in backfat at around 180 days of age (0.57). This confirms that plasma oestradiol can be used as a proxy for androstenone at the standard slaughter age. From Principal Component Analyses, it seems that fat androstenone at 180 days of age is independent from health score, behaviour toward human, libido and semen characteristics. These results suggest that a selection for lower androstenone in fat or plasma oestradiol in pigs at a standard slaughter age would have very-limited impact on libido or semen production capacities of mature boars.
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- 2020
3. Lactation and gestation controls on calcium isotopic compositions in a mammalian model
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Doryan Grivault, Samuel Le Goff, Vincent Balter, Jean-Alexis Hernandez, Auguste Hassler, Emmanuelle Albalat, Stéphane Ferchaud, Jeremy E. Martin, Théo Tacail, Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement (LGL-TPE), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Unité Expérimentale Elevages Porcins Innovants (GenESI), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Oslo (UiO), Center for Earth Evolution and Dynamics, University of Oslo, School of Earth Sciences [Bristol], University of Bristol [Bristol], Laboratoire de Géologie de Lyon - Terre, Planètes, Environnement [Lyon] (LGL-TPE), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,Calcium Isotopes ,Swine ,media_common.quotation_subject ,[SDV]Life Sciences [q-bio] ,Biophysics ,chemistry.chemical_element ,[SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny ,Fractionation ,Calcium ,010502 geochemistry & geophysics ,01 natural sciences ,Biochemistry ,Models, Biological ,Bone and Bones ,Biomaterials ,03 medical and health sciences ,Animal science ,Pregnancy ,Lactation ,[SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology ,medicine ,Animals ,0105 earth and related environmental sciences ,media_common ,Isotope ,Metals and Alloys ,Animal Feed ,Body Fluids ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Animals, Newborn ,Chemistry (miscellaneous) ,Gestation ,Colostrum ,Composition (visual arts) ,Female ,Reproduction - Abstract
Lactation and gestation are among the physiological events that trigger the most intense changes in body calcium (Ca) fluxes. Along with the composition of the animal 2021 diet, these events are suspected to impact the Ca isotopic composition of Ca body reservoirs but their dynamics are poorly understood. In this study, we monitored a group of domestic sows across a full reproduction cycle. We collected tissues and fluids (blood, urine, milk, colostrum, umbilical blood, adult and piglet bones) at different steps of gestation and lactation, and analyzed their Ca isotopic compositions (i.e. δ44/42Ca) by means of multi-collector inductively coupled plasma mass spectrometry. Among other results, we report the first observations of Ca isotopic fractionation between maternal and umbilical blood (Δ44/42Caumbilical blood-sow blood = −0.18 ± 0.11‰, n = 3). Our data also highlight that gestation and lactation periods are characterized by small diet-bone Ca isotopic offsets (Δ44/42Cabone-diet = −0.28 ± 0.11‰, n = 3), with 44Ca-enriched blood compositions during nursing (Δ44/42Canursing blood-gestation blood = $+ 0.42{\rm{\,\,}}_{ - 0.12}^{ + 0.11}$‰, n = 3). Under the light of an up-to-date mammalian box model, we explored different scenarios of gestation and lactation Ca fluxes experienced by a sow-like animal. These simulations suggest that gestation changes on body δ44/42Ca values may result from the intensification of Ca absorption by the animal, whereas the production of 44Ca-depleted milk is the main driver for the 44Ca enrichment in blood during lactation. In addition, our results also support that bone mineralization could be associated with a more restricted Ca isotopic fractionation than previously envisioned. Together, these results refine the framework of Ca isotope applications, notably regarding the monitoring of human bone balance and the study of species and ecosystems from the present and the past.
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- 2020
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4. Steroidome and metabolome analysis in gilt saliva to identify potential biomarkers of boar effect receptivity
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L. Haddad, Doryan Grivault, Cécile Douet, Philippe Liere, Jonathan Savoie, Armelle Prunier, Ghylène Goudet, Stéphane Ferchaud, Antoine Roinsard, A. Pianos, F. Montigny, Florence Maupertuis, Sylviane Boulot, Lydie Nadal-Desbarats, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Imagerie et cerveau (iBrain - Inserm U1253 - UNIV Tours ), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité Expérimentale Elevages Porcins Innovants (GenESI), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INSERM U1195, Unité Expérimentale de Physiologie Animale de l‘Orfrasiére (UE PAO), Chambre Régionale d'Agriculture des Pays de la Loire, Institut Technique de l'Agriculture Biologique (ITAB), Institut du Porc (IFIP), AgriBio4, Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Goudet, Ghylène, Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
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Male ,endocrine system ,Saliva ,BOAR ,Porcine ,Swine ,040301 veterinary sciences ,[SDV]Life Sciences [q-bio] ,media_common.quotation_subject ,Androstenediol ,Biology ,Male effect ,SF1-1100 ,Gas Chromatography-Mass Spectrometry ,0403 veterinary science ,chemistry.chemical_compound ,Animal science ,Metabolites ,Metabolome ,Animals ,Sexual Maturation ,Ovulation ,media_common ,2. Zero hunger ,Estrous cycle ,urogenital system ,0402 animal and dairy science ,Reproducibility of Results ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,Animal culture ,[SDV] Life Sciences [q-bio] ,chemistry ,Steroids ,Female ,Animal Science and Zoology ,Reproduction ,Biomarkers ,Hormone - Abstract
International audience; Optimal management of gilt reproduction requires oestrus synchronization. Hormonal treatments are used for this purpose, but there is a growing demand for non-hormonal alternatives, especially in organic farms. The boar effect is an important alternative opportunity to induce and synchronize oestrus without hormones. Before puberty, gilts exhibit a 'waiting period' during which boar exposure could induce and synchronize the first ovulation. We searched for salivary biomarkers of this period of boar effect receptivity to improve detection of the gilts to stimulate with the perspective of enhancing the efficacy of the boar effect. Saliva samples were collected from 30 Large-White×Landrace crossbred gilts between 140 and 175 days of age. Gilts were exposed twice a day to a boar and subjected to oestrus detection from 150 to 175 days of age. Among the 30 gilts, 10 were detected in oestrus 4 to 7 days after the first introduction of the boar and were considered receptive to the boar effect, 14 were detected in oestrus more than 8 days after first boar contact, and six did not show oestrus and were considered non-receptive. Saliva samples from six receptive and six non-receptive gilts were analyzed for steroidome and for metabolome using gas chromatography coupled to tandem mass spectrometry and 1H nuclear magnetic resonance spectroscopy, respectively. Four saliva samples per gilt were analyzed: 25 days and 11 days before boar introduction, the day of boar introduction, 3 days later for receptive gilts or 7 days later for non-receptive gilts. Twenty-nine steroids and 31 metabolites were detected in gilt saliva. Salivary concentrations of six steroids and three metabolites were significantly different between receptive and non-receptive gilts: progesterone and glycolate 25 days before boar introduction, 3α5β20α- and 3β5α20β-hexahydroprogesterone, dehydroepiandrosterone, androstenediol, succinate, and butyrate 11 days before boar introduction, and 3β5α-tetrahydroprogesterone on the day of boar introduction. Thus, nine potential salivary biomarkers of boar effect receptivity were identified in our experimental conditions. Further studies with higher numbers of gilts and salivary sampling points are necessary to ascertain their reliability.
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- 2021
- Full Text
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5. Analyse transcriptomique cellule-unique pour définir les populations cellulaires pluripotentes et extra-embryonnaires et leurs interactions dans le blastocyste porcin
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Hervé Acloque, Yoann Bailly, Sarah Djebali, Stéphane Ferchaud, Doryan Grivault, Patrick Manceau, claire kuchly, Frédéric Martins, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Génétique, Expérimentation et Système Innovants (GenESI), Institut National de la Recherche Agronomique (INRA), Génome et Transcriptome - Plateforme Génomique (GeT-PlaGe), Institut National de la Recherche Agronomique (INRA)-Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3)
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Animal biology ,population cellulaire ,blastocyste ,Vertebrate Zoology ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Biologie animale ,[SDV.BA.ZV]Life Sciences [q-bio]/Animal biology/Vertebrate Zoology ,cellule unique ,cellule pluripotente ,Zoologie des vertébrés ,ComputingMilieux_MISCELLANEOUS ,étude transcriptomique ,porc - Abstract
National audience
- Published
- 2018
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