28 results on '"Doumandji, Zahra"'
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2. Les bébéparticules
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Doumandji, Zahra, primary
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- 2020
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3. Genes expression profiling of alveolar macrophages exposed to non-functionalized, anionic and cationic multi-walled carbon nanotubes shows three different mechanisms of toxicity
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Nahle, Sara, Cassidy, Hilary, Leroux, Mélanie M., Mercier, Reuben, Ghanbaja, Jaafar, Doumandji, Zahra, Matallanas, David, Rihn, Bertrand H., Joubert, Olivier, and Ferrari, Luc
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- 2020
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4. Correction to: Protein and lipid homeostasis altered in rat macrophages after exposure to metallic oxide nanoparticles
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Doumandji, Zahra, Safar, Ramia, Lovera-Leroux, Mélanie, Nahle, Sara, Cassidy, Hilary, Matallanas, David, Rihn, Bertrand, Ferrari, Luc, and Joubert, Olivier
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- 2020
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5. Nanoparticles and Viruses as Mitophagy Inducers in Immune Cells
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Eidi, Housam, primary, Doumandji, Zahra, additional, Tomljenovic, Lucija, additional, and Rihn, Bertrand Henri, additional
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- 2017
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6. Toxicity of TiO2 Nanoparticles: Validation of Alternative Models
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Leroux, Mélanie M., Doumandji, Zahra, Chézeau, Laetitia, Gaté, Laurent, Nahle, Sara, Hocquel, Romain, Zhernovkov, Vadim, Migot, Sylvie, Ghanbaja, Jafar, Bonnet, Céline, Schneider, Raphaël, Rihn, Bertrand H., Ferrari, Luc, Joubert, Olivier, Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut national de recherche et de sécurité (Vandoeuvre lès Nancy) (INRS ( Vandoeuvre lès Nancy)), University College Dublin [Dublin] (UCD), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Laboratoire Réactions et Génie des Procédés (LRGP), and Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL)
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titanium dioxide ,technology, industry, and agriculture ,[CHIM.MATE]Chemical Sciences/Material chemistry ,macrophages ,lcsh:Chemistry ,transcriptomics ,ALI ,lcsh:Biology (General) ,lcsh:QD1-999 ,toxicogenomics ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,nanoparticles ,rat ,lcsh:QH301-705.5 - Abstract
There are many studies concerning titanium dioxide (TiO2) nanoparticles (NP) toxicity. Nevertheless, there are few publications comparing in vitro and in vivo exposure, and even less comparing air&ndash, liquid interface exposure (ALI) with other in vitro and in vivo exposures. The identification and validation of common markers under different exposure conditions are relevant for the development of smart and quick nanotoxicity tests. In this work, cell viability was assessed in vitro by WST-1 and LDH assays after the exposure of NR8383 cells to TiO2 NP sample. To evaluate in vitro gene expression profile, NR8383 cells were exposed to TiO2 NP during 4 h at 3 cm2 of TiO2 NP/cm2 of cells or 19 &mu, g/mL, in two settings&mdash, submerged cultures and ALI. For the in vivo study, Fischer 344 rats were exposed by inhalation to a nanostructured aerosol at a concentration of 10 mg/m3, 6 h/day, 5 days/week for 4 weeks. This was followed immediately by gene expression analysis. The results showed a low cytotoxic potential of TiO2 NP on NR8383 cells. Despite the absence of toxicity at the doses studied, the different exposures to TiO2 NP induce 18 common differentially expressed genes (DEG) which are involved in mitosis regulation, cell proliferation and apoptosis and inflammation transport of membrane proteins. Among these genes, we noticed the upregulation of Ccl4, Osm, Ccl7 and Bcl3 genes which could be suggested as early response biomarkers after exposure to TiO2 NP. On the other hand, the comparison of the three models helped us to validate the alternative ones, namely submerged and ALI approaches.
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- 2020
7. Prediction of Chronic Inflammation for Inhaled Particles: the Impact of Material Cycling and Quarantining in the Lung Epithelium
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Joubert, Olivier, Kokot, Hana, Kokot, Boštjan, Sebastijanović, Aleksandar, Voss, Carola, Podlipec, Rok, Zawilska, Patrycja, Berthing, Trine, Ballester-López, Carolina, Danielsen, Pernille Høgh, Contini, Claudia, Ivanov, Mikhail, Krišelj, Ana, Čotar, Petra, Zhou, Qiaoxia, Ponti, Jessica, Zhernovkov, Vadim, Schneemilch, Matthew, Doumandji, Zahra, Pušnik, Mojca, Umek, Polona, Pajk, Stane, Schmid, Otmar, Urbančič, Iztok, Irmler, Martin, Beckers, Johannes, Lobaskin, Vladimir, Halappanavar, Sabina, Quirke, Nick, Lyubartsev, Alexander, Vogel, Ulla, Koklič, Tilen, Stoeger, Tobias, Štrancar, Janez, Ballester‐López, Carolina, Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Commission of the European Communities
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MESH: Inflammation ,Technology ,material safety and health hazards ,Chemistry, Multidisciplinary ,[SDV]Life Sciences [q-bio] ,02 engineering and technology ,Advanced materials ,01 natural sciences ,09 Engineering ,Epithelium ,NANOPARTICLES ,General Materials Science ,Lung ,02 Physical Sciences ,Single exposure ,Chemistry, Physical ,Physics ,HAZARD ,advanced microscopies ,021001 nanoscience & nanotechnology ,Material development ,adverse outcome pathways ,3. Good health ,ddc ,Chemistry ,Physics, Condensed Matter ,CHEMICAL SAFETY ,Inhalation ,MESH: Particulate Matter ,Advanced Microscopies ,Adverse Outcome Pathways ,Disease Prediction ,Material Safety And Health Hazards ,Mode Of Action ,Mechanics of Materials ,Lung epithelium ,Physical Sciences ,Science & Technology - Other Topics ,MESH: Inhalation ,Safety ,medicine.symptom ,03 Chemical Sciences ,0210 nano-technology ,NANOTOXICOLOGY ,disease prediction ,Materials science ,In silico ,Materials Science ,Materials Science, Multidisciplinary ,Inflammation ,Computational biology ,Predictive toxicology ,010402 general chemistry ,Physics, Applied ,ACTIN ,mode of action ,MESH: Computer Simulation ,Toxicity Tests ,medicine ,Computer Simulation ,MESH: Lung ,EXPOSURE ,MESH: Particle Size ,Nanoscience & Nanotechnology ,Particle Size ,MESH: Toxicity Tests ,NANOMATERIALS ,Science & Technology ,Mechanical Engineering ,MESH: Chronic Disease ,MESH: Safety ,0104 chemical sciences ,MESH: Epithelium ,MOLECULAR-DYNAMICS ,Chronic Disease ,Particulate Matter ,MATTER ,RESPONSES - Abstract
International audience; On a daily basis, people are exposed to a multitude of health-hazardous airborne particulate matter with notable deposition in the fragile alveolar region of the lungs. Hence, there is a great need for identification and prediction of material-associated diseases, currently hindered due to the lack of in-depth understanding of causal relationships, in particular between acute exposures and chronic symptoms. By applying advanced microscopies and omics to in vitro and in vivo systems, together with in silico molecular modeling, it is determined herein that the long-lasting response to a single exposure can originate from the interplay between the newly discovered nanomaterial quarantining and nanomaterial cycling between different lung cell types. This new insight finally allows prediction of the spectrum of lung inflammation associated with materials of interest using only in vitro measurements and in silico modeling, potentially relating outcomes to material properties for a large number of materials, and thus boosting safe-by-design-based material development. Because of its profound implications for animal-free predictive toxicology, this work paves the way to a more efficient and hazard-free introduction of numerous new advanced materials into our lives.
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- 2020
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8. Prediction of Chronic Inflammation for Inhaled Particles : the Impact of Material Cycling and Quarantining in the Lung Epithelium
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Kokot, Hana, Kokot, Boštjan, Sebastijanović, Aleksandar, Voss, Carola, Podlipec, Rok, Zawilska, Patrycja, Berthing, Trine, Ballester‐López, Carolina, Høgh Danielsen, Pernille, Contini, Claudia, Ivanov, Mikhail, Krišelj, Ana, Čotar, Petra, Zhou, Qiaoxia, Ponti, Jessica, Zhernovkov, Vadim, Schneemilch, Matthew, Doumandji, Zahra, Pušnik, Mojca, Umek, Polona, Pajk, Stane, Joubert, Olivier, Schmid, Otmar, Urbančič, Iztok, Irmler, Martin, Beckers, Johannes, Lobaskin, Vladimir, Halappanavar, Sabina, Quirke, Nick, Lyubartsev, Alexander P., Vogel, Ulla, Koklič, Tilen, Stoeger, Tobias, Štrancar, Janez, Kokot, Hana, Kokot, Boštjan, Sebastijanović, Aleksandar, Voss, Carola, Podlipec, Rok, Zawilska, Patrycja, Berthing, Trine, Ballester‐López, Carolina, Høgh Danielsen, Pernille, Contini, Claudia, Ivanov, Mikhail, Krišelj, Ana, Čotar, Petra, Zhou, Qiaoxia, Ponti, Jessica, Zhernovkov, Vadim, Schneemilch, Matthew, Doumandji, Zahra, Pušnik, Mojca, Umek, Polona, Pajk, Stane, Joubert, Olivier, Schmid, Otmar, Urbančič, Iztok, Irmler, Martin, Beckers, Johannes, Lobaskin, Vladimir, Halappanavar, Sabina, Quirke, Nick, Lyubartsev, Alexander P., Vogel, Ulla, Koklič, Tilen, Stoeger, Tobias, and Štrancar, Janez
- Abstract
On a daily basis, people are exposed to a multitude of health-hazardous airborne particulate matter with notable deposition in the fragile alveolar region of the lungs. Hence, there is a great need for identification and prediction of material-associated diseases, currently hindered due to the lack of in-depth understanding of causal relationships, in particular between acute exposures and chronic symptoms. By applying advanced microscopies and omics to in vitro and in vivo systems, together with in silico molecular modeling, it is determined herein that the long-lasting response to a single exposure can originate from the interplay between the newly discovered nanomaterial quarantining and nanomaterial cycling between different lung cell types. This new insight finally allows prediction of the spectrum of lung inflammation associated with materials of interest using only in vitro measurements and in silico modeling, potentially relating outcomes to material properties for a large number of materials, and thus boosting safe-by-design-based material development. Because of its profound implications for animal-free predictive toxicology, this work paves the way to a more efficient and hazard-free introduction of numerous new advanced materials into our lives.
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- 2020
- Full Text
- View/download PDF
9. Disease Prediction: Prediction of Chronic Inflammation for Inhaled Particles: the Impact of Material Cycling and Quarantining in the Lung Epithelium (Adv. Mater. 47/2020)
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Kokot, Hana, primary, Kokot, Boštjan, additional, Sebastijanović, Aleksandar, additional, Voss, Carola, additional, Podlipec, Rok, additional, Zawilska, Patrycja, additional, Berthing, Trine, additional, Ballester‐López, Carolina, additional, Danielsen, Pernille Høgh, additional, Contini, Claudia, additional, Ivanov, Mikhail, additional, Krišelj, Ana, additional, Čotar, Petra, additional, Zhou, Qiaoxia, additional, Ponti, Jessica, additional, Zhernovkov, Vadim, additional, Schneemilch, Matthew, additional, Doumandji, Zahra, additional, Pušnik, Mojca, additional, Umek, Polona, additional, Pajk, Stane, additional, Joubert, Olivier, additional, Schmid, Otmar, additional, Urbančič, Iztok, additional, Irmler, Martin, additional, Beckers, Johannes, additional, Lobaskin, Vladimir, additional, Halappanavar, Sabina, additional, Quirke, Nick, additional, Lyubartsev, Alexander P., additional, Vogel, Ulla, additional, Koklič, Tilen, additional, Stoeger, Tobias, additional, and Štrancar, Janez, additional
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- 2020
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10. Prediction of Chronic Inflammation for Inhaled Particles: the Impact of Material Cycling and Quarantining in the Lung Epithelium
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Kokot, Hana, primary, Kokot, Boštjan, additional, Sebastijanović, Aleksandar, additional, Voss, Carola, additional, Podlipec, Rok, additional, Zawilska, Patrycja, additional, Berthing, Trine, additional, Ballester‐López, Carolina, additional, Danielsen, Pernille Høgh, additional, Contini, Claudia, additional, Ivanov, Mikhail, additional, Krišelj, Ana, additional, Čotar, Petra, additional, Zhou, Qiaoxia, additional, Ponti, Jessica, additional, Zhernovkov, Vadim, additional, Schneemilch, Matthew, additional, Doumandji, Zahra, additional, Pušnik, Mojca, additional, Umek, Polona, additional, Pajk, Stane, additional, Joubert, Olivier, additional, Schmid, Otmar, additional, Urbančič, Iztok, additional, Irmler, Martin, additional, Beckers, Johannes, additional, Lobaskin, Vladimir, additional, Halappanavar, Sabina, additional, Quirke, Nick, additional, Lyubartsev, Alexander P., additional, Vogel, Ulla, additional, Koklič, Tilen, additional, Stoeger, Tobias, additional, and Štrancar, Janez, additional
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- 2020
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11. Validation of an air/liquid interface device for TiO2 nanoparticle toxicity assessment on NR8383 cells: preliminary results
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Leroux, Mélanie M, primary, Doumandji, Zahra, additional, Chezeau, Laetitia, additional, Hocquel, Romain, additional, Ferrari, Luc, additional, Joubert, Olivier, additional, Rihn, Phèdre, additional, and Rihn, Bertrand H, additional
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- 2020
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12. From the Roundabout of Molecular Events to Nanomaterial-Induced Chronic Inflammation Prediction
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Majaron, Hana, primary, Kokot, Boštjan, additional, Sebastijanović, Aleksandar, additional, Voss, Carola, additional, Podlipec, Rok, additional, Zawilska, Patrycja, additional, Berthing, Trine, additional, López, Carolina Ballester, additional, Danielsen, Pernille Høgh, additional, Contini, Claudia, additional, Ivanov, Mikhail, additional, Krišelj, Ana, additional, Čotar, Petra, additional, Zhou, Qiaoxia, additional, Ponti, Jessica, additional, Zhernovkov, Vadim, additional, Schneemilch, Matthew, additional, Doumandji, Zahra, additional, Pušnik, Mojca, additional, Umek, Polona, additional, Pajk, Stane, additional, Joubert, Olivier, additional, Schmid, Otmar, additional, Urbančič, Iztok, additional, Irmler, Martin, additional, Beckers, Johannes, additional, Lobaskin, Vladimir, additional, Halappanavar, Sabina, additional, Quirke, Nick, additional, Lyubartsev, Alexander P., additional, Vogel, Ulla, additional, Koklič, Tilen, additional, Stoeger, Tobias, additional, and Štrancar, Janez, additional
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- 2020
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13. Correction to: Protein and lipid homeostasis altered in rat macrophages after exposure to metallic oxide nanoparticles
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Doumandji, Zahra, primary, Safar, Ramia, additional, Lovera-Leroux, Mélanie, additional, Nahle, Sara, additional, Cassidy, Hilary, additional, Matallanas, David, additional, Rihn, Bertrand, additional, Ferrari, Luc, additional, and Joubert, Olivier, additional
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- 2019
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14. Cytotoxicity and global transcriptional responses induced by zinc oxide nanoparticles NM 110 in PMA-differentiated THP-1 cells
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Safar, Ramia, Doumandji, Zahra, Saidou, Timeh, Ferrari, Luc, Nahle, Sara, Rihn, Bertrand, Joubert, Olivier, Nutrition-Génétique et Exposition aux Risques Environnementaux (NGERE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Institut Jean Lamour (IJL), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), CCSD, Accord Elsevier, and Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
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Dose-Response Relationship, Drug ,Cell Survival ,Macrophages ,[SDV]Life Sciences [q-bio] ,Down-Regulation ,Cell Differentiation ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Monocytes ,Cell Line ,Up-Regulation ,[SDV] Life Sciences [q-bio] ,[SDV.TOX] Life Sciences [q-bio]/Toxicology ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,Humans ,Nanoparticles ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Particle Size ,Zinc Oxide ,Transcriptome ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology - Abstract
International audience; Despite a wide production and use of zinc oxide nanoparticles (ZnONP), their toxicological study is only of limited number and their impact at a molecular level is seldom addressed. Thus, we have used, as a model, zinc oxide nanoparticle NM110 (ZnO110NP) exposure to PMA-differentiated THP-1 macrophages. The cell viability was studied at the cellular level using WST-1, LDH and Alamar Blue® assays, as well as at the molecular level by transcriptomic analysis. Exposure of cells to ZnO110NP for 24 h decreased their viability in a dose-dependent manner with mean inhibitory concentrations (IC50) of 8.1 µg/mL. Transcriptomic study of cells exposed to two concentrations of ZnO110NP: IC50 and a quarter of it (IC50/4) for 4 h showed that the expressions of genes involved in metal metabolism are perturbed. In addition, expression of genes acting in transcription regulation and DNA binding, as well as clusters of genes related to protein synthesis and structure were altered. It has to be noted that the expressions of metallothioneins genes (MT1, MT2) and genes of heat-shock proteins genes (HSP) were strongly upregulated for both conditions. These genes might be used as an early marker of exposition to ZnONP. On the contrary, at IC50 exposure, modifications of gene expression involved in inflammation, apoptosis and mitochondrial suffering were noted indicating a less specific cellular response. Overall, this study brings a resource of transcriptional data for ZnONP toxicity for further mechanistic studies.
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- 2019
15. Identification de marqueurs d’exposition et d’effets de nanoparticules métalliques sur modèle in vitro
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Doumandji, Zahra, Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Lorraine, Olivier Joubert, European Project: 686098,H2020,H2020-NMP-2015-two-stage,SmartNanoTox(2016), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), UL, Thèses, and Smart Tools for Gauging Nano Hazards - SmartNanoTox - - H20202016-03-01 - 2020-02-29 - 686098 - VALID
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Inflammation ,[CHIM.MATE] Chemical Sciences/Material chemistry ,Profil d’expression de gènes et de protéines ,Zinc iron oxide ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,[CHIM.MATE]Chemical Sciences/Material chemistry ,Genes and proteins expression profile ,Oxyde de zinc ,[SDV.TOX] Life Sciences [q-bio]/Toxicology ,Nickel zinc iron oxide ,Metallic nanoparticles ,Stress oxydant ,Oxidative stress ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Zinc oxide ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Nanoparticules métalliques ,Nickel zinc fer oxyde ,Zinc fer oxyde ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology - Abstract
As a consequence of the extension of the use of nanoparticles in different industrial sectors, the number of potentially exposed workers continues to grow, without fully knowing the toxicological properties of these materials. Since nanoparticles can be aerosolized in the occupational atmosphere, inhalation is the major occupational exposure route. For this reason, risk assessment of exposure to nanomaterials requires toxicology studies to be conducted on cellular models of the airways. In this manuscript, the cellular and molecular responses of rat alveolar macrophages (NR8383) exposed to metallic oxide nanoparticles: ZnO, ZnFe2O4, NiZnFe2O4, Fe2O3, TiO2-NM105 and TiO2-NRCWE001, were studied, combining conventional toxicological analyzes (characterization of nanoparticles by transmission electron microscopy and dynamic light scattering, evaluation of cytotoxicity by WST-1 assays and LDH release); and high throughput molecular screening (transcriptomic and proteomic analyzes). NR8383 cells were exposed to the ZnO, ZnFe2O4, NiZnFe2O4, Fe2O3, TiO2-NM105 and TiO2-NRCWE001 nanoparticles for 24 h which allowed for the determination of a sub-toxic dose for each nanoparticle to which the macrophages were exposed for molecular analysis. Four hours after exposue NR8383 to nanoparticles, many genes and proteins were differentially expressed. Oxidative stress was the adverse biological response following exposure of cells to nanoparticles composed of zinc. In contrast, inflammation was the main activated pathway in cells exposed to the anatase and rutile form of TiO2 nanoparticles. In conclusion, this study exposes the "biological fingerprints" of the two groups of nanoparticles of interest. Finally, our study, combined with previous literature studies, could also be beneficial in validating biomarkers of exposure and effects of nanomaterials suggested in order to predict adverse biological effects., En conséquence de l'extension de l’utilisation des nanoparticules dans différents secteurs industriels, le nombre de travailleurs potentiellement exposés ne cesse de croître, sans parfaitement connaître les propriétés toxicologiques de ces matériaux. Etant donné que les nanoparticules peuvent se trouver en suspension dans l’atmosphère professionnelle, l'inhalation représente une voie d'exposition professionnelle majeure. De ce fait, l’évaluation des risques liés à l’exposition aux nanomatériaux requiert d’entreprendre des études de toxicologie sur des modèles cellulaires des voies aériennes. Dans ce manuscrit, les réponses cellulaires et moléculaires des macrophages alvéolaires de rat (NR8383) exposés à des nanoparticules d’oxydes métalliques : ZnO, ZnFe2O4, NiZnFe2O4, Fe2O3, TiO2-NM105 et TiO2-NRCWE001, ont été étudiées, en combinant des analyses toxicologiques classiques (caractérisation des nanoparticules par microscopie électronique à transmission et par diffusion dynamique de la lumière, évaluation de la cytotoxicité par tests WST-1 et libération de LDH); et de criblage moléculaire à haut débit (analyses de transcriptomique et de protéomique). Des cellules NR8383 ont été exposées aux nanoparticules ZnO, ZnFe2O4, NiZnFe2O4, Fe2O3, TiO2-NM105 et TiO2-NRCWE001 pendant 24 h ce qui a permis de déterminer une dose sub-toxique pour chaque nanoparticule à laquelle les macrophages ont été exposés pour l’analyse moléculaire. Quatre heures suite à l’exposition des cellules aux nanoparticules, de nombreux gènes et protéines étaient différentiellemment exprimés. Le stress oxydant était la réponse biologique adverse suite à l’exposition des cellules aux nanoparticules composées de zinc. En revanche, l’inflammation était la principale voie activée dans les cellules exposées à la forme anatase et rutile des nanoparticules de TiO2. En conclusion, cette étude expose les « empreintes biologiques » des deux groupes de nanoparticules d’intérêt. Enfin, notre étude combinée à des travaux antérieurs de la littérature pourraient aussi être profitables pour valider les biomarqueurs d’exposition et d’effets aux nanomatériaux suggérés afin de prédire les effets biologiques adverses.
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- 2019
16. Single wall and multiwall carbon nanotubes induce different toxicological responses in rat alveolar macrophages
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Nahle, Sara, Safar, Ramia, Grandemange, Stéphanie, Foliguet, Bernard, Lovera-Leroux, Mélanie, Doumandji, Zahra, Le Faou, Alain, Joubert, Olivier, Rihn, Bertrand, Ferrari, Luc, Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Cibles thérapeutiques, formulation et expertise pré-clinique du médicament (CITHEFOR), Université de Lorraine (UL), Centre de Recherche en Automatique de Nancy (CRAN), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Service de Foetopathologie et placentologie [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Faculté de Médecine [Nancy], and Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)
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Air Pollutants ,Nanotubes, Carbon ,Surface Properties ,multiwall carbon nanotubes ,MWCNT ,apoptosis ,single wall carbon nanotubes ,in vitro ,Cell Line ,Rats ,inflammation ,SWCNT ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Macrophages, Alveolar ,Animals ,oxidative stress ,rat macrophages cell line NR8383 ,Particle Size ,ComputingMilieux_MISCELLANEOUS ,Research Articles ,Research Article - Abstract
Human exposure to airborne carbon nanotubes (CNT) is increasing because of their applications in different sectors; therefore, they constitute a biological hazard. Consequently, developing studies on CNT toxicity become a necessity. CNTs can have different properties in term of length, size and charge. Here, we compared the cellular effect of multiwall (MWCNTs) and single wall CNTs (SWCNTs). MWCNTs consist of multiple layers of graphene, while SWCNTs are monolayers. The effects of MWCNTs and SWCNTs were evaluated by the water‐soluble tetrazolium salt cell proliferation assay on NR8383 cells, rat alveolar macrophage cell line (NR8383). After 24 hours of exposure, MWCNTs showed higher toxicity (50% inhibitory concentration [IC50] = 3.2 cm2/cm2) than SWCNTs (IC50 = 44 cm2/cm2). Only SWCNTs have induced NR8383 cells apoptosis as assayed by flow cytometry using the annexin V/IP staining test. The expression of genes involved in oxidative burst (Ncf1), inflammation (Nfκb, Tnf‐α, Il‐6 and Il‐1β), mitochondrial damage (Opa) and apoptotic balance (Pdcd4, Bcl‐2 and Casp‐8) was determined. We found that MWCNT exposure predominantly induce inflammation, while SWCNTs induce apoptosis and impaired mitochondrial function. Our results clearly suggest that MWCNTs are ideal candidates for acute inflammation induction. In vivo studies are required to confirm this hypothesis. However, we conclude that toxicity of CNTs is dependent on their physical and chemical characteristics., The physical and chemical properties of carbon nanotubes (CNTs) are of interest in many fields, particularly in the biomedical one. Consequently, human exposure to different CNTs is growing. Therefore, using NR8383 cells, rat alveolar macrophages, we evaluated modifications in gene expression after exposure to multiwall CNTs (NM403) and single wall CNTs (NRCWE‐055). We found different NR8383 cell responses, which confirm that CNT toxicity depends on physical and chemical characteristics of CNTs.
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- 2019
17. Protein and lipid homeostasis altered in rat macrophages after exposure to metallic oxide nanoparticles
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Doumandji, Zahra, Safar, Ramia, Lovera-Leroux, Mélanie, Nahle, Sara, Cassidy, Hilary, Matallanas, David, Rihn, Bertrand, Ferrari, Luc, Joubert, Olivier, Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Nutrition-Génétique et Exposition aux Risques Environnementaux (NGERE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), and University College Dublin [Dublin] (UCD)
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Proteomics ,Iron oxide nanoparticles ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,Original Article ,Zinc oxide nanoparticles ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,Transcriptomics ,NR8383 ,Zinc iron oxide nanoparticles ,ComputingMilieux_MISCELLANEOUS - Abstract
Metal oxide nanoparticles (NPs), such as ZnO, ZnFe2O4, and Fe2O3, are widely used in industry. However, little is known about the cellular pathways involved in their potential toxicity. Here, we particularly investigated the key molecular pathways that are switched on after exposure to sub-toxic doses of ZnO, ZnFe2O4, and Fe2O3 in the in vitro rat alveolar macrophages (NR8383). As in our model, the calculated IC50 were respectively 16, 68, and more than 200 μg/mL for ZnO, ZnFe2O4, and Fe2O3; global gene and protein expression profiles were only analyzed after exposure to ZnO and ZnFe2O4 NPs. Using a rat genome microarray technology, we found that 985 and 1209 genes were significantly differentially expressed in NR8383 upon 4 h exposure to ¼ IC50 of ZnO and ZnFe2O4 NPs, respectively. It is noteworthy that metallothioneins were overexpressed genes following exposure to both NPs. Moreover, Ingenuity Pathway Analysis revealed that the top canonical pathway disturbed in NR8383 exposed to ZnO and ZnFe2O4 NPs was eIF2 signaling involved in protein homeostasis. Quantitative mass spectrometry approach performed from both NR8383 cell extracts and culture supernatant indicated that 348 and 795 proteins were differentially expressed upon 24 h exposure to ¼ IC50 of ZnO and ZnFe2O4 NPs, respectively. Bioinformatics analysis revealed that the top canonical pathways disturbed in NR8383 were involved in protein homeostasis and cholesterol biosynthesis for both exposure conditions. While VEGF signaling was specific to ZnO exposure, iron homeostasis signaling pathway was specific to ZnFe2O4 NPs. Overall, the study provides resource of transcriptional and proteomic markers of response to ZnO and ZnFe2O4 NP-induced toxicity through combined transcriptomics, proteomics, and bioinformatics approaches. Electronic supplementary material The online version of this article (10.1007/s10565-019-09484-6) contains supplementary material, which is available to authorized users.
- Published
- 2019
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18. CdTe0.5S0.5/ZnS Quantum Dots Embedded in a Molecularly Imprinted Polymer for the Selective Optosensing of Dopamine
- Author
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Khadem-Abbassi, Kiana, primary, Rinnert, Hervé, additional, Balan, Lavinia, additional, Doumandji, Zahra, additional, Joubert, Olivier, additional, Masteri-Farahani, Majid, additional, and Schneider, Raphaël, additional
- Published
- 2019
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19. Disruption of protein homeostasis: a potential biomarker of exposure to three metal oxide nanoparticles
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Doumandji, Zahra M., primary, Rihn, Bertrand, additional, and Joubert, Olivier, additional
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- 2018
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- View/download PDF
20. Anti-cancer Activity and Gene Expression Responses to Methanol Extract of Gladiolus segetum in THP-1 Human Monocytic Leukemia Cells
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Eddine, Marref Salah, primary, Naima, Benkiki, additional, Akram, Melakhessou Mohamed, additional, Joubert, Olivier, additional, Doumandji, Zahra Manel, additional, and Safar, Ramia, additional
- Published
- 2018
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- View/download PDF
21. Gene Expression Levels of Selected Factors in Monocytic Leukemia Cell Line THP-1 Upon Treatment with n-butanol Extract of Atractylis flava Desf against Cancer
- Author
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Akram, Melakhessou Mohamed, primary, Naima, Benkiki, additional, Eddine, Marref Salah, additional, Joubert, Olivier, additional, Doumandji, Zahra Manel, additional, and Safar, Ramia, additional
- Published
- 2018
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- View/download PDF
22. Anti-cancer Activity and Gene Expression Responses to Methanol Extract of Gladiolus segetum in THP-1 Human Monocytic Leukemia Cells.
- Author
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Eddine, Marref Salah, Naima, Benkiki, Akram, Melakhessou Mohamed, Joubert, Olivier, Doumandji, Zahra Manel, and Safar, Ramia
- Subjects
CELL survival ,ELECTRON-transfer catalysis ,LEUKEMIA ,MONOCYTES ,GENE expression - Abstract
Objective: To Evaluate the viability of cells against exposure to the Methanol Extract of Gladiolus segetum (MEGS) by various colorimetric detection tests (Alamar Blue, WST-1) and the trypan blue exclusion test in Human Leukemia Monocytes THP-1 and Using reverse transcription-polymerase chain reaction assays. Methods: Human THP-1 monocytic cell line was exposed to 25 to 800 μg/mL concentrations of MEGS for 24 h and cellular viability was estimated using Alamar Blue, WST-1 and trypan blue tests. Gene expressions were performed using reverse transcription-polymerase chain reaction assays and the measurement of Caspase-3 enzyme activity. Results: A decrease of viability was observed with a dose-dependent effect of MEGS on THP-1. In addition, a differential expression involved in the different genes tested. Moreover, it has been shown overexpression of casp3 for an exposure to 25 μg/ml and 100 μg/ml of MEGS. Conclusion: Our results show that the MEGS present a toxicity to THP-1 cells, especially with regard to apoptosis processes. [ABSTRACT FROM AUTHOR]
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- 2019
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23. Gene Expression Levels of Selected Factors in Monocytic Leukemia Cell Line THP-1 Upon Treatment with n-butanol Extract of Atractylis flava Desf against Cancer.
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Akram, Melakhessou Mohamed, Naima, Benkiki, Eddine, Marref Salah, Joubert, Olivier, Doumandji, Zahra Manel, and Safar, Ramia
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CELL lines ,CELL survival ,GENE expression ,LEUKEMIA ,EXPRESSIVE behavior - Abstract
Objective: The aim of the present study was to screen the anticancer activity of Atractylis flava Desf in butanolic extract (AFBE). In addition, we attempted to investigate the gene expression levels of seven relevant genes involved in the pathways leading to toxicity: oxidative stress (NCF1, OPA1, SDHA), inflammation (TNFα), apoptotic balance (PDCD4, BCL2, CASP8) and the measurement of caspase-3 activity on the acute monocytic leukemia cell line THP-1. Material and Methods: The cell viability was assessed using Trypan blue exclusion, alamarBlue® and WST-1 assays. The gene expression levels were tested by RT-qPCR. Activity of caspase-3 activity was performed using the EnzChek® Caspase-3 Assay kit to confirm the induction of apoptosis on tumor cells. Results: We observed significant growth inhibitory activity of the AFBE extract on the acute monocytic leukemia cell line THP-1. Moreover, the gene expression analysis showed that the plant extract caused statistically significant downregulation of selected genes compared to the untreated cells. Caspase-3 activity in the treated cells was significantly elevated. Conclusion: The presents study states that the butanolic extract of Atractylis flava Desf showed a significant cytotoxic effect against THP-1 cells. Our results suggest that the AFBE may be beneficial for the treatment and prevention of cancer. [ABSTRACT FROM AUTHOR]
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- 2019
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24. P-143 - Disruption of protein homeostasis: a potential biomarker of exposure to three metal oxide nanoparticles
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Doumandji, Zahra M., Rihn, Bertrand, and Joubert, Olivier
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- 2018
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25. Toxicity of TiO 2 Nanoparticles: Validation of Alternative Models.
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Leroux MM, Doumandji Z, Chézeau L, Gaté L, Nahle S, Hocquel R, Zhernovkov V, Migot S, Ghanbaja J, Bonnet C, Schneider R, Rihn BH, Ferrari L, and Joubert O
- Subjects
- Administration, Inhalation, Aerosols toxicity, Animals, Apoptosis drug effects, Biomarkers metabolism, Cell Line, Cell Proliferation drug effects, Cell Survival drug effects, Inflammation, Male, Membrane Proteins metabolism, Mitosis drug effects, Nanostructures toxicity, Rats, Rats, Inbred F344, Transcriptome drug effects, Nanoparticles toxicity, Titanium toxicity
- Abstract
There are many studies concerning titanium dioxide (TiO
2 ) nanoparticles (NP) toxicity. Nevertheless, there are few publications comparing in vitro and in vivo exposure, and even less comparing air-liquid interface exposure (ALI) with other in vitro and in vivo exposures. The identification and validation of common markers under different exposure conditions are relevant for the development of smart and quick nanotoxicity tests. In this work, cell viability was assessed in vitro by WST-1 and LDH assays after the exposure of NR8383 cells to TiO2 NP sample. To evaluate in vitro gene expression profile, NR8383 cells were exposed to TiO2 NP during 4 h at 3 cm2 of TiO2 NP/cm2 of cells or 19 μg/mL, in two settings-submerged cultures and ALI. For the in vivo study, Fischer 344 rats were exposed by inhalation to a nanostructured aerosol at a concentration of 10 mg/m3 , 6 h/day, 5 days/week for 4 weeks. This was followed immediately by gene expression analysis. The results showed a low cytotoxic potential of TiO2 NP on NR8383 cells. Despite the absence of toxicity at the doses studied, the different exposures to TiO2 NP induce 18 common differentially expressed genes (DEG) which are involved in mitosis regulation, cell proliferation and apoptosis and inflammation transport of membrane proteins. Among these genes, we noticed the upregulation of Ccl4 , Osm , Ccl7 and Bcl3 genes which could be suggested as early response biomarkers after exposure to TiO2 NP. On the other hand, the comparison of the three models helped us to validate the alternative ones, namely submerged and ALI approaches.- Published
- 2020
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26. Cytotoxicity and global transcriptional responses induced by zinc oxide nanoparticles NM 110 in PMA-differentiated THP-1 cells.
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Safar R, Doumandji Z, Saidou T, Ferrari L, Nahle S, Rihn BH, and Joubert O
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- Cell Differentiation drug effects, Cell Line, Cell Survival drug effects, Dose-Response Relationship, Drug, Down-Regulation, Humans, Macrophages pathology, Monocytes pathology, Nanoparticles chemistry, Particle Size, Up-Regulation, Zinc Oxide chemistry, Macrophages drug effects, Monocytes drug effects, Nanoparticles toxicity, Transcriptome drug effects, Zinc Oxide toxicity
- Abstract
Despite a wide production and use of zinc oxide nanoparticles (ZnONP), their toxicological study is only of limited number and their impact at a molecular level is seldom addressed. Thus, we have used, as a model, zinc oxide nanoparticle NM110 (ZnO110NP) exposure to PMA-differentiated THP-1 macrophages. The cell viability was studied at the cellular level using WST-1, LDH and Alamar Blue
® assays, as well as at the molecular level by transcriptomic analysis. Exposure of cells to ZnO110NP for 24 h decreased their viability in a dose-dependent manner with mean inhibitory concentrations (IC50 ) of 8.1 μg/mL. Transcriptomic study of cells exposed to two concentrations of ZnO110NP: IC50 and a quarter of it (IC50 /4) for 4 h showed that the expressions of genes involved in metal metabolism are perturbed. In addition, expression of genes acting in transcription regulation and DNA binding, as well as clusters of genes related to protein synthesis and structure were altered. It has to be noted that the expressions of metallothioneins genes (MT1, MT2) and genes of heat-shock proteins genes (HSP) were strongly upregulated for both conditions. These genes might be used as an early marker of exposure to ZnONP. On the contrary, at IC50 exposure, modifications of gene expression involved in inflammation, apoptosis and mitochondrial suffering were noted indicating a less specific cellular response. Overall, this study brings a resource of transcriptional data for ZnONP toxicity for further mechanistic studies., (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)- Published
- 2019
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27. CdTe 0.5 S 0.5 /ZnS Quantum Dots Embedded in a Molecularly Imprinted Polymer for the Selective Optosensing of Dopamine.
- Author
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Khadem-Abbassi K, Rinnert H, Balan L, Doumandji Z, Joubert O, Masteri-Farahani M, and Schneider R
- Abstract
This work describes the preparation of molecularly imprinted polymer (MIP)-modified core/shell CdTe
0.5 S0.5 /ZnS quantum dots (QDs). The QDs@MIP particles were used for the selective and sensitive detection of dopamine (DA). Acrylamide, which is able to form hydrogen bonds with DA, and ethylene glycol dimethylacrylate (EGDMA) as cross-linker were used for the preparation of the MIP. Highly cross-linked polymer particles with sizes up to 1 µm containing the dots were obtained after the polymerization. After the removal of the DA template, MIP-modified QDs (QDs@MIP) exhibit a high photoluminescence (PL) with an intensity similar to that of QDs embedded in the nonimprinted polymer (NIP). A linear PL decrease was observed upon addition of DA to QDs@MIP and the PL response was in the linear ranges from 2.63 µM to 26.30 µM with a limit of detection of 6.6 nM. The PL intensity of QDs@MIP was quenched selectively by DA. The QDs@MIP particles developed in this work are easily prepared and of low cost and are therefore of high interest for the sensitive and selective detection of DA in biological samples.- Published
- 2019
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28. Single wall and multiwall carbon nanotubes induce different toxicological responses in rat alveolar macrophages.
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Nahle S, Safar R, Grandemange S, Foliguet B, Lovera-Leroux M, Doumandji Z, Le Faou A, Joubert O, Rihn B, and Ferrari L
- Subjects
- Air Pollutants chemistry, Animals, Cell Line, Nanotubes, Carbon chemistry, Particle Size, Rats, Surface Properties, Air Pollutants toxicity, Macrophages, Alveolar drug effects, Nanotubes, Carbon toxicity
- Abstract
Human exposure to airborne carbon nanotubes (CNT) is increasing because of their applications in different sectors; therefore, they constitute a biological hazard. Consequently, developing studies on CNT toxicity become a necessity. CNTs can have different properties in term of length, size and charge. Here, we compared the cellular effect of multiwall (MWCNTs) and single wall CNTs (SWCNTs). MWCNTs consist of multiple layers of graphene, while SWCNTs are monolayers. The effects of MWCNTs and SWCNTs were evaluated by the water-soluble tetrazolium salt cell proliferation assay on NR8383 cells, rat alveolar macrophage cell line (NR8383). After 24 hours of exposure, MWCNTs showed higher toxicity (50% inhibitory concentration [IC
50 ] = 3.2 cm2 /cm2 ) than SWCNTs (IC50 = 44 cm2 /cm2 ). Only SWCNTs have induced NR8383 cells apoptosis as assayed by flow cytometry using the annexin V/IP staining test. The expression of genes involved in oxidative burst (Ncf1), inflammation (Nfκb, Tnf-α, Il-6 and Il-1β), mitochondrial damage (Opa) and apoptotic balance (Pdcd4, Bcl-2 and Casp-8) was determined. We found that MWCNT exposure predominantly induce inflammation, while SWCNTs induce apoptosis and impaired mitochondrial function. Our results clearly suggest that MWCNTs are ideal candidates for acute inflammation induction. In vivo studies are required to confirm this hypothesis. However, we conclude that toxicity of CNTs is dependent on their physical and chemical characteristics., (© 2019 The Authors Journal of Applied Toxicology Published by John Wiley & Sons Ltd.)- Published
- 2019
- Full Text
- View/download PDF
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