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4. Spatial N-glycan rearrangement on α5β1 integrin nucleates galectin-3 oligomers to determine endocytic fate α

Author

Shafaq-Zadah, Massiullah, primary, Dransart, Estelle, additional, Wunder, Christian, additional, Chambon, Valérie, additional, Valades-Cruz, Cesar Augusto, additional, Leconte, Ludovic, additional, Kumar Sarangi, Nirod, additional, Robinson, Jack, additional, Bai, Siau-Kun, additional, Regmi, Raju, additional, Di Cicco, Aurélie, additional, Hovasse, Agnès, additional, Bartels, Richard, additional, Nilsson, Ulf, additional, Cianférani-Sanglier, Sarah, additional, Leffler, Hakon, additional, Keyes, Tia, additional, Lévy, Daniel, additional, Raunser, Stefan, additional, Roderer, Daniel, additional, and Johannes, Ludger, additional

Published
2023
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7. Growth factor-induced desialylation for the fast control of endocytosis

Author

MacDonald, Ewan, primary, Forrester, Alison, additional, Valades-Cruz, Cesar Augusto, additional, Madsen, Thomas D, additional, Hetmanski, Joseph, additional, Dransart, Estelle, additional, Ng, Yeap, additional, Godbole, Rashmi, additional, Akhil Shp, Ananthan, additional, Leconte, Ludovic, additional, Chambon, Valerie, additional, Ghosh, Debarpan, additional, Pinet, Alexis, additional, Bhatia, Dhiraj D, additional, Lombard, Berangere, additional, Loew, Damarys, additional, Larsen, Martin R, additional, Leffler, Hakon, additional, Lefeber, Dirk, additional, Clausen, Henrik, additional, Caswell, Patrick T, additional, Shafaq-Zadah, Massiullah, additional, Mayor, Satyajit, additional, Weigert, Roberto, additional, Wunder, Christian, additional, and Johannes, Ludger, additional

Published
2023
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12. Supplementary Figure S3 from Synergy of Radiotherapy and a Cancer Vaccine for the Treatment of HPV-Associated Head and Neck Cancer

Author

Mondini, Michele, primary, Nizard, Mevyn, primary, Tran, Thi, primary, Mauge, Laetitia, primary, Loi, Mauro, primary, Clémenson, Céline, primary, Dugue, Delphine, primary, Maroun, Pierre, primary, Louvet, Emilie, primary, Adam, Julien, primary, Badoual, Cécile, primary, Helley, Dominique, primary, Dransart, Estelle, primary, Johannes, Ludger, primary, Vozenin, Marie-Catherine, primary, Perfettini, Jean-Luc, primary, Tartour, Eric, primary, and Deutsch, Eric, primary

Published
2023
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13. Data from Synergy of Radiotherapy and a Cancer Vaccine for the Treatment of HPV-Associated Head and Neck Cancer

Author

Mondini, Michele, primary, Nizard, Mevyn, primary, Tran, Thi, primary, Mauge, Laetitia, primary, Loi, Mauro, primary, Clémenson, Céline, primary, Dugue, Delphine, primary, Maroun, Pierre, primary, Louvet, Emilie, primary, Adam, Julien, primary, Badoual, Cécile, primary, Helley, Dominique, primary, Dransart, Estelle, primary, Johannes, Ludger, primary, Vozenin, Marie-Catherine, primary, Perfettini, Jean-Luc, primary, Tartour, Eric, primary, and Deutsch, Eric, primary

Published
2023
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19. Supplementary methods from A Therapeutic Her2/neu Vaccine Targeting Dendritic Cells Preferentially Inhibits the Growth of Low Her2/neu–Expressing Tumor in HLA-A2 Transgenic Mice

Author

Tran, Thi, primary, Diniz, Mariana O., primary, Dransart, Estelle, primary, Gey, Alain, primary, Merillon, Nathalie, primary, Lone, Yu Chun, primary, Godefroy, Sylvie, primary, Sibley, Craig, primary, Ferreira, Luis CS, primary, Medioni, Jacques, primary, Oudard, Stephane, primary, Johannes, Ludger, primary, and Tartour, Eric, primary

Published
2023
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20. Supplementary Table 2 from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer

Author

Badoual, Cécile, primary, Hans, Stéphane, primary, Merillon, Nathalie, primary, Van Ryswick, Cordélia, primary, Ravel, Patrice, primary, Benhamouda, Nadine, primary, Levionnois, Emeline, primary, Nizard, Mevyn, primary, Si-Mohamed, Ali, primary, Besnier, Nicolas, primary, Gey, Alain, primary, Rotem-Yehudar, Rinat, primary, Pere, Hélène, primary, Tran, Thi, primary, Guerin, Coralie L., primary, Chauvat, Anne, primary, Dransart, Estelle, primary, Alanio, Cécile, primary, Albert, Sebastien, primary, Barry, Beatrix, primary, Sandoval, Federico, primary, Quintin-Colonna, Françoise, primary, Bruneval, Patrick, primary, Fridman, Wolf H., primary, Lemoine, Francois M., primary, Oudard, Stephane, primary, Johannes, Ludger, primary, Olive, Daniel, primary, Brasnu, Daniel, primary, and Tartour, Eric, primary

Published
2023
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21. Supplementary Figure 5 from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer

Author

Badoual, Cécile, primary, Hans, Stéphane, primary, Merillon, Nathalie, primary, Van Ryswick, Cordélia, primary, Ravel, Patrice, primary, Benhamouda, Nadine, primary, Levionnois, Emeline, primary, Nizard, Mevyn, primary, Si-Mohamed, Ali, primary, Besnier, Nicolas, primary, Gey, Alain, primary, Rotem-Yehudar, Rinat, primary, Pere, Hélène, primary, Tran, Thi, primary, Guerin, Coralie L., primary, Chauvat, Anne, primary, Dransart, Estelle, primary, Alanio, Cécile, primary, Albert, Sebastien, primary, Barry, Beatrix, primary, Sandoval, Federico, primary, Quintin-Colonna, Françoise, primary, Bruneval, Patrick, primary, Fridman, Wolf H., primary, Lemoine, Francois M., primary, Oudard, Stephane, primary, Johannes, Ludger, primary, Olive, Daniel, primary, Brasnu, Daniel, primary, and Tartour, Eric, primary

Published
2023
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22. Supplementary Methods and Materials from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer

Author

Badoual, Cécile, primary, Hans, Stéphane, primary, Merillon, Nathalie, primary, Van Ryswick, Cordélia, primary, Ravel, Patrice, primary, Benhamouda, Nadine, primary, Levionnois, Emeline, primary, Nizard, Mevyn, primary, Si-Mohamed, Ali, primary, Besnier, Nicolas, primary, Gey, Alain, primary, Rotem-Yehudar, Rinat, primary, Pere, Hélène, primary, Tran, Thi, primary, Guerin, Coralie L., primary, Chauvat, Anne, primary, Dransart, Estelle, primary, Alanio, Cécile, primary, Albert, Sebastien, primary, Barry, Beatrix, primary, Sandoval, Federico, primary, Quintin-Colonna, Françoise, primary, Bruneval, Patrick, primary, Fridman, Wolf H., primary, Lemoine, Francois M., primary, Oudard, Stephane, primary, Johannes, Ludger, primary, Olive, Daniel, primary, Brasnu, Daniel, primary, and Tartour, Eric, primary

Published
2023
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23. Supplementary Figure 3 from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer

Author

Badoual, Cécile, primary, Hans, Stéphane, primary, Merillon, Nathalie, primary, Van Ryswick, Cordélia, primary, Ravel, Patrice, primary, Benhamouda, Nadine, primary, Levionnois, Emeline, primary, Nizard, Mevyn, primary, Si-Mohamed, Ali, primary, Besnier, Nicolas, primary, Gey, Alain, primary, Rotem-Yehudar, Rinat, primary, Pere, Hélène, primary, Tran, Thi, primary, Guerin, Coralie L., primary, Chauvat, Anne, primary, Dransart, Estelle, primary, Alanio, Cécile, primary, Albert, Sebastien, primary, Barry, Beatrix, primary, Sandoval, Federico, primary, Quintin-Colonna, Françoise, primary, Bruneval, Patrick, primary, Fridman, Wolf H., primary, Lemoine, Francois M., primary, Oudard, Stephane, primary, Johannes, Ludger, primary, Olive, Daniel, primary, Brasnu, Daniel, primary, and Tartour, Eric, primary

Published
2023
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24. Supplementary Figure 2 from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer

Author

Badoual, Cécile, primary, Hans, Stéphane, primary, Merillon, Nathalie, primary, Van Ryswick, Cordélia, primary, Ravel, Patrice, primary, Benhamouda, Nadine, primary, Levionnois, Emeline, primary, Nizard, Mevyn, primary, Si-Mohamed, Ali, primary, Besnier, Nicolas, primary, Gey, Alain, primary, Rotem-Yehudar, Rinat, primary, Pere, Hélène, primary, Tran, Thi, primary, Guerin, Coralie L., primary, Chauvat, Anne, primary, Dransart, Estelle, primary, Alanio, Cécile, primary, Albert, Sebastien, primary, Barry, Beatrix, primary, Sandoval, Federico, primary, Quintin-Colonna, Françoise, primary, Bruneval, Patrick, primary, Fridman, Wolf H., primary, Lemoine, Francois M., primary, Oudard, Stephane, primary, Johannes, Ludger, primary, Olive, Daniel, primary, Brasnu, Daniel, primary, and Tartour, Eric, primary

Published
2023
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25. Supplementary Figure 4 from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer

Author

Badoual, Cécile, primary, Hans, Stéphane, primary, Merillon, Nathalie, primary, Van Ryswick, Cordélia, primary, Ravel, Patrice, primary, Benhamouda, Nadine, primary, Levionnois, Emeline, primary, Nizard, Mevyn, primary, Si-Mohamed, Ali, primary, Besnier, Nicolas, primary, Gey, Alain, primary, Rotem-Yehudar, Rinat, primary, Pere, Hélène, primary, Tran, Thi, primary, Guerin, Coralie L., primary, Chauvat, Anne, primary, Dransart, Estelle, primary, Alanio, Cécile, primary, Albert, Sebastien, primary, Barry, Beatrix, primary, Sandoval, Federico, primary, Quintin-Colonna, Françoise, primary, Bruneval, Patrick, primary, Fridman, Wolf H., primary, Lemoine, Francois M., primary, Oudard, Stephane, primary, Johannes, Ludger, primary, Olive, Daniel, primary, Brasnu, Daniel, primary, and Tartour, Eric, primary

Published
2023
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26. Supplementary Figure 6 from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer

Author

Badoual, Cécile, primary, Hans, Stéphane, primary, Merillon, Nathalie, primary, Van Ryswick, Cordélia, primary, Ravel, Patrice, primary, Benhamouda, Nadine, primary, Levionnois, Emeline, primary, Nizard, Mevyn, primary, Si-Mohamed, Ali, primary, Besnier, Nicolas, primary, Gey, Alain, primary, Rotem-Yehudar, Rinat, primary, Pere, Hélène, primary, Tran, Thi, primary, Guerin, Coralie L., primary, Chauvat, Anne, primary, Dransart, Estelle, primary, Alanio, Cécile, primary, Albert, Sebastien, primary, Barry, Beatrix, primary, Sandoval, Federico, primary, Quintin-Colonna, Françoise, primary, Bruneval, Patrick, primary, Fridman, Wolf H., primary, Lemoine, Francois M., primary, Oudard, Stephane, primary, Johannes, Ludger, primary, Olive, Daniel, primary, Brasnu, Daniel, primary, and Tartour, Eric, primary

Published
2023
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27. Supplementary Table 1 from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer

Author

Badoual, Cécile, primary, Hans, Stéphane, primary, Merillon, Nathalie, primary, Van Ryswick, Cordélia, primary, Ravel, Patrice, primary, Benhamouda, Nadine, primary, Levionnois, Emeline, primary, Nizard, Mevyn, primary, Si-Mohamed, Ali, primary, Besnier, Nicolas, primary, Gey, Alain, primary, Rotem-Yehudar, Rinat, primary, Pere, Hélène, primary, Tran, Thi, primary, Guerin, Coralie L., primary, Chauvat, Anne, primary, Dransart, Estelle, primary, Alanio, Cécile, primary, Albert, Sebastien, primary, Barry, Beatrix, primary, Sandoval, Federico, primary, Quintin-Colonna, Françoise, primary, Bruneval, Patrick, primary, Fridman, Wolf H., primary, Lemoine, Francois M., primary, Oudard, Stephane, primary, Johannes, Ludger, primary, Olive, Daniel, primary, Brasnu, Daniel, primary, and Tartour, Eric, primary

Published
2023
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28. Supplementary Figure 1 from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer

Author

Badoual, Cécile, primary, Hans, Stéphane, primary, Merillon, Nathalie, primary, Van Ryswick, Cordélia, primary, Ravel, Patrice, primary, Benhamouda, Nadine, primary, Levionnois, Emeline, primary, Nizard, Mevyn, primary, Si-Mohamed, Ali, primary, Besnier, Nicolas, primary, Gey, Alain, primary, Rotem-Yehudar, Rinat, primary, Pere, Hélène, primary, Tran, Thi, primary, Guerin, Coralie L., primary, Chauvat, Anne, primary, Dransart, Estelle, primary, Alanio, Cécile, primary, Albert, Sebastien, primary, Barry, Beatrix, primary, Sandoval, Federico, primary, Quintin-Colonna, Françoise, primary, Bruneval, Patrick, primary, Fridman, Wolf H., primary, Lemoine, Francois M., primary, Oudard, Stephane, primary, Johannes, Ludger, primary, Olive, Daniel, primary, Brasnu, Daniel, primary, and Tartour, Eric, primary

Published
2023
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32. SLC3A2 N-glycosylation and alternate evolutionary trajectories for amino acid metabolism

Author

Zhang, Cunjie, primary, Shafaq-Zadah, Massiullah, additional, Pawling, Judy, additional, Ng, Deanna Wan Jie, additional, Hesketh, Geoffrey G., additional, Dransart, Estelle, additional, Pacholczyk, Karina, additional, Longo, Joseph, additional, Gingras, Anne-Claude, additional, Penn, Linda Z., additional, Johannes, Ludger, additional, and Dennis, James W., additional

Published
2022
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34. A CCR4 antagonist combined with vaccines induces antigen-specific CD8+ T cells and tumor immunity against self antigens

Author

Pere, Helene, Montier, Yves, Bayry, Jagadeesh, Quintin-Colonna, Francoise, Merillon, Nathalie, Dransart, Estelle, Badoual, Cecile, Gey, Alain, Ravel, Patrice, Marcheteau, Elie, Batteux, Frederic, Sandoval, Federico, Adotevi, Olivier, Chiu, Christopher, Garcia, Sylvie, Tanchot, Corinne, Lone, Yu-Chun, Ferreira, Luis Carlos, Nelson, Brad H., Hanahan, Douglas, Fridman, Wolf Herman, Johannes, Ludger, and Tartour, Eric

Published
2011
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36. CXCR6 deficiency impairs cancer vaccine efficacy and CD8+ resident memory T-cell recruitment in head and neck and lung tumors

Author

Karaki, Soumaya, Blanc, Charlotte, Tran, Thi, Galy-Fauroux, Isabelle, Mougel, Alice, Dransart, Estelle, Anson, Marie, Tanchot, Corinne, Paolini, Lea, Gruel, Nadege, Gibault, Laure, Lepimpec-Barhes, Francoise, Fabre, Elizabeth, Benhamouda, Nadine, Badoual, Cecile, Damotte, Diane, Donnadieu, Emmanuel, Kobold, Sebastian, Mami-Chouaib, Fathia, Golub, Rachel, Johannes, Ludger, Tartour, Eric, Vougny, Marie-Christine, Université Sorbonne Paris Cité - - USPC2011 - ANR-11-IDEX-0005 - IDEX - VALID, Paris-Centre de Recherche Cardiovasculaire (PARCC (UMR_S 970/ U970)), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Equipe labellisée Ligue contre le Cancer, Chimie biologique des membranes et ciblage thérapeutique (CBMCT - UMR 3666 / U1143), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Unité de génétique et biologie des cancers (U830), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Recherche Translationnelle, Institut Curie [Paris], Service d’anatomo‑pathologie [AP-HP Hôpital Européen Georges Pompidou] (Centre de Ressources biologiques), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Center for Integrated Protein Science (CIPSM), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Ludwig-Maximilians-Universität München (LMU)-Helmholtz Zentrum München = German Research Center for Environmental Health, German Center for Lung Research - DZL [Munich, Germany], Immunologie intégrative des tumeurs et immunothérapie des cancers (INTIM), Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Lymphopoïèse (Lymphopoïèse (UMR_1223 / U1223 / U-Pasteur_4)), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), This work has been funded by Fondation ARC, INCA PLBio, Labex Immuno-Oncology, Site intégré de recherche en cancérologie (SIRIC CARPEM, SIRIC Curie), Cancéropole d’Ile de France, Carnot Curie Cancer, FONCER. SK is supported by the European Research Council (grant 756017, ARMOR-T)., ANR-11-IDEX-0005,USPC,Université Sorbonne Paris Cité(2011), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC), Département de recherche translationnelle, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM)-Helmholtz-Zentrum München (HZM)-Ludwig Maximilian University of Munich [Germany] (LMU München), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), École Pratique des Hautes Études (EPHE), and Technical University of Munich (TUM)-Ludwig Maximilian University of Munich [Germany] (LMU München)-Helmholtz-Zentrum München (HZM)

Subjects
Lung Neoplasms, [SDV.IMM] Life Sciences [q-bio]/Immunology, Vaccine Efficacy, immunization, Cancer Vaccines, Memory T Cells, Lymphocytes, Tumor-Infiltrating, Cell Line, Tumor, Tumor Microenvironment, Animals, Humans, Administration, Intranasal, RC254-282, Receptors, CXCR6, Mice, Knockout, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Basic Tumor Immunology, Chemokine CXCL16, adaptive immunity, vaccination, Tumor Burden, Mice, Inbred C57BL, Phenotype, Head and Neck Neoplasms, CD8-positive T-lymphocytes, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, Immunologic Memory
Abstract

International audience; Background Resident memory T lymphocytes (TRM) are located in tissues and play an important role in immunosurveillance against tumors. The presence of TRM prior to treatment or their induction is associated to the response to anti-Programmed cell death protein 1 (PD-1)/Programmed death-ligand 1 (PD-L1) immunotherapy and the efficacy of cancer vaccines. Previous work by our group and others has shown that the intranasal route of vaccination allows more efficient induction of these cells in head and neck and lung mucosa, resulting in better tumor protection. The mechanisms of in vivo migration of these cells remains largely unknown, apart from the fact that they express the chemokine receptor CXCR6.Methods We used CXCR6-deficient mice and an intranasal tumor vaccination model targeting the Human Papillomavirus (HPV) E7 protein expressed by the TC-1 lung cancer epithelial cell line. The role of CXCR6 and its ligand, CXCL16, was analyzed using multiparametric cytometric techniques and Luminex assays.Human biopsies obtained from patients with lung cancer were also included in this study.Results We showed that CXCR6 was preferentially expressed by CD8+ TRM after vaccination in mice and also on intratumoral CD8+ TRM derived from human lung cancer. We also demonstrate that vaccination of Cxcr6-deficient mice induces a defect in the lung recruitment of antigen-specific CD8+ T cells, preferentially in the TRM subsets. In addition, we found that intranasal vaccination with a cancer vaccine is less effective in these Cxcr6-deficient mice compared with wild-type mice, and this loss of efficacy is associated with decreased recruitment of local antitumor CD8+ TRM. Interestingly, intranasal, but not intramuscular vaccination induced higher and more sustained concentrations of CXCL16, compared with other chemokines, in the bronchoalveolar lavage fluid and pulmonary parenchyma.Conclusions This work demonstrates the in vivo role of CXCR6-CXCL16 axis in the migration of CD8+ resident memory T cells in lung mucosa after vaccination, resulting in the control of tumor growth. This work reinforces and explains why the intranasal route of vaccination is the most appropriate strategy for inducing these cells in the head and neck and pulmonary mucosa, which remains a major objective to overcome resistance to anti-PD-1/PD-L1, especially in cold tumors.

Published
2021

38. Local IFNα enhances the anti-tumoral efficacy of systemic anti-PD1 to prevent tumor relapse

Author

Guerin, Marion v, Regnier, Fabienne, Thoreau, Maxime, Vimeux, Lene, Benard, Matthieu, Dransart, Estelle, Penny, Hweixian L, Johannes, Ludger, Trautmann, Alain, Bercovici, Nadege, [Institut Cochin] Departement Infection, immunité, inflammation, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Chimie biologique des membranes et ciblage thérapeutique (CBMCT - UMR 3666 / U1143), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC), Singapore Immunology Network (SIgN), Biomedical Sciences Institute (BMSI), BERCOVICI, NADEGE, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
lymphocytes, [SDV]Life Sciences [q-bio], Interferon-alpha, Basic Tumor Immunology, tumor-infiltrating, programmed cell death 1 receptor, macrophages, [SDV] Life Sciences [q-bio], Disease Models, Animal, Mice, interferon inducers, Neoplasms, Animals, Humans, immunotherapy
Abstract

International audience; Background Tumor relapse constitutes a major challenge for anti-tumoral treatments, including immunotherapies. Indeed, most cancer-related deaths occur during the tumor relapse phase. Methods We designed a mouse model of tumor relapse in which mice transplanted with E7 + TC1 tumor cells received a single therapeutic vaccination of STxB-E7+IFNα. Unlike the complete regression observed after two vaccinations, such a treatment induced a transient shrinkage of the tumor mass, followed by a rapid tumor outgrowth. To prevent this relapse, we tested the efficacy of a local administration of IFNα together with a systemic therapy with anti-PD1 Ab. The immune response was analyzed during both the tumor regression and relapse phases. Results We show that, during the regression phase, tumors of mice treated with a single vaccination of STxB-E7 + IFNα harbor fewer activated CD8 T cells and monocytes than tumors doomed to fully regress after two vaccinations. In contrast, the systemic injection of an anti-PD1 Ab combined with the peri-tumoral injection of IFNα in this time frame promotes infiltration of activated CD8 T cells and myeloid cells, which, together, exert a high cytotoxicity in vitro against TC1 cells. Moreover, the IFNα and anti-PD1 Ab combination was found to be more efficient than IFNα or anti-PD1 used alone in preventing tumor relapse and was better able to prolong mice survival. Conclusions Together, these results indicate that the local increase of IFNα in combination with an anti-PD1 therapy is an effective way to promote efficient and durable innate and adaptive immune responses preventing tumor relapse.

Published
2020

39. Galectin-3 Binding to α5β1Integrin in Pore Suspended Biomembranes

Author

Sarangi, Nirod Kumar, Shafaq-Zadah, Massiullah, Berselli, Guilherme B., Robinson, Jack, Dransart, Estelle, Di Cicco, Aurélie, Lévy, Daniel, Johannes, Ludger, and Keyes, Tia E.

Abstract

Galectin-3 (Gal3) is a β-galactoside binding lectin that mediates many physiological functions, including the binding of cells to the extracellular matrix for which the glycoprotein α5β1integrin is of critical importance. The mechanisms by which Gal3 interacts with membranes have not been widely explored to date due to the complexity of cell membranes and the difficulty of integrin reconstitution within model membranes. Herein, to study their interaction, Gal3 and α5β1were purified, and the latter reconstituted into pore-suspended lipid bilayers comprised eggPC:eggPA. Using electrochemical impedance and fluorescence lifetime correlation spectroscopy, we found that on incubation with low nanomolar concentrations of wild-type Gal3, the membrane’s admittance and fluidity, as well as integrin’s lateral diffusivity, were enhanced. These effects were diminished in the following conditions: (i) absence of integrin, (ii) presence of lactose as a competitive inhibitor of glycan–Gal3 interaction, and (iii) use of a Gal3 mutant that lacked the N-terminal oligomerization domain (Gal3ΔNter). These findings indicated that WTGal3 oligomerized on α5β1integrin in a glycan-dependent manner and that the N-terminal domain interacted directly with membranes in a way that is yet to be fully understood. At concentrations above 10 nM of WTGal3, membrane capacitance started to decrease and very slowly diffusing molecular species appeared, which indicated the formation of protein clusters made from WTGal3−α5β1integrin assemblies. Overall, our study demonstrates the capacity of WTGal3 to oligomerize in a cargo protein-dependent manner at low nanomolar concentrations. Of note, these WTGal3 oligomers appeared to have membrane active properties that could only be revealed using our sensitive methods. At slightly higher WTGal3 concentrations, the capacity to generate lateral assemblies between cargo proteins was observed. In cells, this could lead to the construction of tubular endocytic pits according to the glycolipid–lectin (GL–Lect) hypothesis or to the formation of galectin lattices, depending on cargo glycoprotein stability at the membrane, the local Gal3 concentration, or plasma membrane intrinsic parameters. The study also demonstrates the utility of microcavity array-suspended lipid bilayers to address the biophysics of transmembrane proteins.

Published
2022
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40. Site‐specific N‐glycan profiles of α5β1 integrin from rat liver.

Author

Mirgorodskaya, Ekaterina, Dransart, Estelle, Shafaq‐Zadah, Massiullah, Roderer, Daniel, Sihlbom, Carina, Leffler, Hakon, and Johannes, Ludger

Subjects
*MEMBRANE glycoproteins, *GLYCOLIPIDS, *INTEGRINS, *GLYCANS, *GLYCOPROTEINS, *LIVER, *FUCOSYLATION, *RATS
Abstract

Background Information: Like most other cell surface proteins, α5β1 integrin is glycosylated, which is required for its various activities in ways that mostly remain to be determined. Results: Here, we have established the first comprehensive site‐specific glycan map of α5β1 integrin that was purified from a natural source, that is, rat liver. This analysis revealed striking site selective variations in glycan composition. Complex bi, tri, or tetraantennary N‐glycans were predominant at various proportions at most potential N‐glycosylation sites. A few of these sites were nonglycosylated or contained high mannose or hybrid glycans, indicating that early N‐glycan processing was hindered. Almost all complex N‐glycans had fully galactosylated and sialylated antennae. Moderate levels of core fucosylation and high levels of O‐acetylation of NeuAc residues were observed at certain sites. An O‐linked HexNAc was found in an EGF‐like domain of β1 integrin. The extensive glycan information that results from our study was projected onto a map of α5β1 integrin that was obtained by homology modeling. We have used this model for the discussion of how glycosylation might be used in the functional cycle of α5β1 integrin. A striking example concerns the involvement of glycan‐binding galectins in the regulation of the molecular homeostasis of glycoproteins at the cell surface through the formation of lattices or endocytic pits according to the glycolipid‐lectin (GL‐Lect) hypothesis. Conclusion: We expect that the glycoproteomics data of the current study will serve as a resource for the exploration of structural mechanisms by which glycans control α5β1 integrin activity and endocytic trafficking. Significance: Glycosylation of α5β1 integrin has been implicated in multiple aspects of integrin function and structure. Yet, detailed knowledge of its glycosylation, notably the specific sites of glycosylation, is lacking. Furthermore, the α5β1 integrin preparation that was analyzed here is from a natural source, which is of importance as there is not a lot of literature in the field about the glycosylation of "native" glycoproteins. [ABSTRACT FROM AUTHOR]

Published
2022
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44. Induction of resident memory T cells enhances the efficacy of cancer vaccine

Author

Nizard, Mevyn, primary, Roussel, Hélène, additional, Diniz, Mariana O., additional, Karaki, Soumaya, additional, Tran, Thi, additional, Voron, Thibault, additional, Dransart, Estelle, additional, Sandoval, Federico, additional, Riquet, Marc, additional, Rance, Bastien, additional, Marcheteau, Elie, additional, Fabre, Elizabeth, additional, Mandavit, Marion, additional, Terme, Magali, additional, Blanc, Charlotte, additional, Escudie, Jean-Baptiste, additional, Gibault, Laure, additional, Barthes, Françoise Le Pimpec, additional, Granier, Clemence, additional, Ferreira, Luis C. S., additional, Badoual, Cecile, additional, Johannes, Ludger, additional, and Tartour, Eric, additional

Published
2017
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45. A Therapeutic Her2/neu Vaccine Targeting Dendritic Cells Preferentially Inhibits the Growth of Low Her2/neu–Expressing Tumor in HLA-A2 Transgenic Mice

Author

Tran, Thi, primary, Diniz, Mariana O., additional, Dransart, Estelle, additional, Gey, Alain, additional, Merillon, Nathalie, additional, Lone, Yu Chun, additional, Godefroy, Sylvie, additional, Sibley, Craig, additional, Ferreira, Luis CS, additional, Medioni, Jacques, additional, Oudard, Stephane, additional, Johannes, Ludger, additional, and Tartour, Eric, additional

Published
2016
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47. Vaccine-induced tumor regression requires a dynamic cooperation between T cells and myeloid cells at the tumor site

Author

Thoreau, Maxime, primary, Penny, HweiXian Leong, additional, Tan, KarWai, additional, Regnier, Fabienne, additional, Weiss, Julia Miriam, additional, Lee, Bernett, additional, Johannes, Ludger, additional, Dransart, Estelle, additional, Le Bon, Agnès, additional, Abastado, Jean-Pierre, additional, Tartour, Eric, additional, Trautmann, Alain, additional, and Bercovici, Nadège, additional

Published
2015
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49. Retrograde transport is not required for cytosolic translocation of the B-subunit of Shiga toxin

Author

Garcia-Castillo, Maria Daniela, primary, Tran, Thi, additional, Bobard, Alexandre, additional, Renard, Henri-François, additional, Rathjen, Stefan J., additional, Dransart, Estelle, additional, Stechmann, Bahne, additional, Lamaze, Christophe, additional, Lord, Mike, additional, Cintrat, Jean-Christophe, additional, Enninga, Jost, additional, Tartour, Eric, additional, and Johannes, Ludger, additional

Published
2015
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50. Synergy of Radiotherapy and a Cancer Vaccine for the Treatment of HPV-Associated Head and Neck Cancer

Author

Mondini, Michele, primary, Nizard, Mevyn, additional, Tran, Thi, additional, Mauge, Laetitia, additional, Loi, Mauro, additional, Clémenson, Céline, additional, Dugue, Delphine, additional, Maroun, Pierre, additional, Louvet, Emilie, additional, Adam, Julien, additional, Badoual, Cécile, additional, Helley, Dominique, additional, Dransart, Estelle, additional, Johannes, Ludger, additional, Vozenin, Marie-Catherine, additional, Perfettini, Jean-Luc, additional, Tartour, Eric, additional, and Deutsch, Eric, additional

Published
2015
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