1. 2(3),9(10),16(17),23(24)-Octamethoxyphthalocyanine-Cu/CuFe2O4 nanocomposites with enhanced peroxidase-like activity for the sensitive colorimetric determination of glutathione.
- Author
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Shi, Yunxia, Duan, Hui, Du, Meiyan, Kong, Xia, Xue, Ke, Lu, Guang, and Liu, Qingyun
- Subjects
GLUTATHIONE ,CHROMOGENIC compounds ,PEROXIDASE ,NANOCOMPOSITE materials ,CATALYTIC oxidation ,COPPER ,DETECTION limit - Abstract
In this work, 2(3),9(10),16(17),23(24)-octamethoxyphthalocyanine (Pc(OH)
8 ) was first used to modify Cu/CuFe2 O4 (Cu/CFO) nanospheres, and Pc(OH)8 –Cu/CFO nanocomposites with enhanced peroxidase mimetic enzyme activity was obtained compared to the performance of Cu/CFO alone. SEM, XRD, and XPS were used to study the surface morphology, phase composition and elemental analysis of the newly synthesized Pc(OH)8 –Cu/CFO. Using TMB as the chromogenic substrate, Pc(OH)8 –Cu/CFO accelerate the oxidation process of chromogenic substrate 3,3′,5,5′- tetramethylbenzidine (TMB) by hydrogen peroxide in three minutes. The catalytic oxidation mechanism of Pc(OH)8 –Cu/CFO on TMB was comprehensively investigated through free radical and fluorescence probe experiments. Based on the excellent catalytic performance of the composite materials, a simple and fast colorimetric sensing platform for the colorimetric detection of H2 O2 and glutathione (GSH) was constructed in the range of 0.6–5 mM and 2–10 μM with detection limits (LODs) of 0.35 mM and 0.55 μM, respectively. In addition, using Pc(OH)8 –Cu/CFO colorimetric detection for GSH has a lower detection limit, good selectivity and high sensitivity, which is expected to become an ideal material for GSH colorimetric detection. [ABSTRACT FROM AUTHOR]- Published
- 2024
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