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Additional file 1: Fig. S1. Comparison of squalene contents in strains SquC1 and S01. Fig. S2. GC–MS analysis of SQO and SDO. Fig. S3. The concentrations of each product are provided in ERG7-modified strains. Fig. S4. The protein sequences comparison between heterologous lanosterol synthases and ERG7. Fig. S5. Removing the bottleneck to stimulate PTs production. Fig. S6. Detection of 24,25-epoxycucurbitadienol. Fig. S7. Detection of the white solids accumulated on the tank wall during the fed-batch fermentation. Fig. S8. Correlation between samples. Table S1. S. cerevisiae strains used in this work. Table S2. Plasmids used in this study. Table S3. Primers used in this study. Table S4. Heterologous lanosterol synthase sequences.

Objective The treatment of missed Monteggia fracture remains a challenge, despite the various surgical methods described. The purpose of this study was to explore a new surgical technique utilizing external fixator‐assisted ulnar osteotomy and to assess the surgical results in a case series. Methods Thirteen patients with missed Monteggia fractures were treated at our institution using this new surgical technique from August 2012 to January 2016. Our series included 11 boys and 2 girls. The left elbow was involved in 6 patients and the right elbow was involved in 7 patients. According to the Bado classification, 10 fractures were classified as Bado type I with anterior radial head dislocation and 3 were classified as Bado type III with anterolateral dislocation. The average age at the time of surgery was 5 years 8 months (range, 2 years 2 months–10 years). The mean trauma‐to‐surgery interval was 12 months (range, 2–36 months). All patients underwent ulnar osteotomy with angulation and lengthening using a temporary external fixator, plate fixation of the osteotomy, and open reduction of the radial head dislocation without annular ligament reconstruction. Results The average follow‐up was 27 months (range, 16–44 months). The average operation time was 175 min (range, 140–215 min). The average length of distraction was 0.7 cm (range, 0.5–1.2 cm) and the average angulation was 28° (range, 20°–30°) at the ulnar osteotomy site intraoperatively. The elbow performance score (Kim's) was excellent in 10 cases and good in 3 cases. No neurovascular complications, compartment syndrome or implant breakage occurred. No pain in the distal radioulnar joint or limited range of motion of the wrist occurred in any patient. The radial head remained reduced in all patients with no subluxation or redislocation. However, delayed ulnar union occurred in 3 cases, all of which were successfully treated with plaster cast immobilization within approximately 6 months postoperatively. One patient presented with cubitus valgus postoperatively with a carrying angle of 30°, which was 10° greater than the contralateral carrying angle. Conclusions External fixator‐assisted ulnar osteotomy offers substantial flexibility for achieving the optimal positioning of the transected ulna to reduce the radial head prior to the final ulnar osteotomy fixation with a plate, thereby facilitating an effective operative performance. Our procedure is a safe and effective method to treat missed pediatric Monteggia fractures.

This paper presents the mathematical model of membrane current of neuron resulting from electromagnetic radiation based on the foundation of neuronal energy theory; and the effect of electromagnetic radiation on the dynamic behaviors of single neuron and the firing activities of two neurons coupled with gap connection are investigated. Results show that the neuronal firing rate is lowered as the radiation intensity increases, and finally reaches a stable value. As the radiation intensity increases, the periodical spiking of neuron is transformed into bursting firing, which is well explained based on the dynamic bifurcation theory. It turns out that the bursting firing induced by the electromagnetic radiation could spread out in neuronal network through an electrical gap junction.