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ObjectiveTo explore the correlation of sleep quality with negative life events and coping styles in adolescents based on structural equation modeling， and to provide references for improving the adolescents' sleep quality.MethodsFrom December 2021 to May 2022， a total of 767 junior middle school students from three schools in Chongqing were enrolled， and assessed using Pittsburgh Sleep Quality Index （PSQI）， Adolescent Self-rating Life Events Checklist （ASLEC） and Simplified Coping Style Questionnaire （SCSQ）. Then the structural equation model was applied to discuss the correlation of sleep quality with negative life events and coping styles in adolescents.ResultsA total of 222 adolescents （28.94%） were found to have sleep disorders. PSQI score was positively correlated with ASLEC score and negative coping dimension score of SCSQ （r=0.612， 0.590， P

Summary: Silver carp myofibrillar proteins (MP) with different moleculars states were constructed by the addition of NaCl and fracture degree of muscle fibres. Results showed that salt‐dissolved MP (P+) under unfrozen and oxidised state showed higher oxidation degree with higher carbonyl (3.42 nmol mg−1) and dityrosine contents (25.98 a.u.) (P < 0.05) compared with saltless‐polymeric MP (P) and salt‐induced swelling MP (F+) due to addition of salt and broken muscle fibres. After oxidative and freeze–thaw process, P+ group also exhibited more cross‐linking as evidenced by turbidity (0.73), solubility (64.96%) and SDS‐PAGE, but unordered aggregates in P group increased probably due to severe frozen denaturation. Moreover, F+ group of salt‐induced swelling MP from intact muscle fibres suffered less structural damages with higher fluorescence intensity (668.41 a.u.) and higher α‐helix contents (15.56%). Overall, Fish‐ Hua of salt‐dissolved MP had a higher oxidation level and NaCl contributed to improve freeze–thaw stability of MP during freeze–thaw process. [ABSTRACT FROM AUTHOR]

To identify the common inhibition types, the putative decision system is unsatisfactory. In a new decision system, Michaelis-Menten constants and maximal reaction rates were plotted versus inhibitor concentrations for deriving Kik and Kiv as the inhibition constants, respectively; their difference was quantified as the ratio of the larger one to the smaller one. Such ratios below 2.0 suggested uncompetitive inhibitors, over 5.0 suggested noncompetitive or competitive inhibitors, and from 2.0 to 5.0 suggested mixed inhibitors. By the new decision system, (i) the simulation recovery of uncompetitive inhibitors under CVs of 2% or 5% was improved by four times, but that of competitive or noncompetitive inhibitors was improved slightly; (ii) the recovery of L-phenylalanine as an uncompetitive inhibitor of intestinal alkaline phosphatase reached 38%, while the putative decision system lost all; the recovery of xanthine as a competitive inhibitor of uricase was improved slightly. Therefore, the new decision system was better. [ABSTRACT FROM AUTHOR]

Objective: To identify the mayven-interacting proteins and study the effect of mayven on multiple sclerosis pathogenesis., Methods: The full length and four different fragments of mayven gene were amplified by polymerase chain reaction (PCR) with specific primers and were cloned into the yeast expression vector pDBLeu. Then, the recombinant plasmids were transformed into MAV203 yeast strain. The autonomous activation of their expression products was detected., Results: The yeast expression vectors of mayven, which include a full-length and four different fragments, were constructed successfully. Full length P1, fragments P3 and P4 have no effect on the expression of HIS3 and LacZ gene, but fragments P7 and P8 do. The C-terminal of Mayven gene may contain a transcription activation domain., Conclusion: Full length P1, fragments P3 and P4 of the mayven gene can be used to screen the mayven-interacting proteins, but whether Mayven has transcriptional activation activity need to be studied.