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3 results on '"Duan Dan-li"'

1. Combined immunity of DNA vector and recombinant vaccinia virus expressing Gag proteins of equine infectious anemia virus

Author

Shao Yiming, Dai Chunming, Duan Dan-li, Wang Shuhui, Shen Rong-xian, Zhang Xiao-yan, and Liu Ying

Subjects
Virus quantification, Multidisciplinary, biology, animal diseases, viruses, biochemical phenomena, metabolism, and nutrition, Group-specific antigen, biology.organism_classification, Recombinant virus, Virology, Molecular biology, Virus, law.invention, Equine infectious anemia, chemistry.chemical_compound, chemistry, law, Recombinant DNA, Vaccinia, Homologous recombination
Abstract

In order to develop a new vaccine candidate for equine infectious anemia virus (EIAV), gag gene of Chinese donkey leukocyte attenuated strain (EIAV DLV) and its parental virulent strain (EIAV LN) were inserted respectively into the TK region of the Tiantan strain (VV) of vaccinia virus by homologous recombination and the positive clone was confirmed by blue plaque assay. Protein expression was examined by Western blot. Prime and prime-boost procedures were used to immunize mice with two DNA vectors and two recombinant vaccinia viruses expressing EIAV Gag proteins. The results showed that the specific lysis of CTL responses in the DNA+rVV groups was stronger than those in the DNA groups, amounting to 31%. Although the levels of specific antibodies were not significantly different, we could conclude that the recombinant vaccinia virus could boost the cellular responses following DNA vector priming. There was no detectable difference between the immune responses induced by DLV and LN Gag proteins. This data demonstrates that the combined immunity of DNA vector and recombinant vaccinia virus expressing EIAV gag proteins, utilizing the prime-boost procedure, can drive immunized mice to produce powerful cellular responses. These results lay an important foundation for the development of a new EIAV genetic engineering vaccine.

Published
2004

2. [Immunogenicity analysis of HIV vaccine based on replicating vaccinia virus Tiantan vector].

Author

Liu Y, Duan DL, Peng H, Tang HL, Liu S, Zhang N, Wang N, Liu JY, and Shao YM

Subjects
AIDS Vaccines genetics, Animals, Female, Genetic Vectors immunology, Immunization, Interferon-gamma blood, Mice, Mice, Inbred BALB C, Rabbits, Recombination, Genetic, Species Specificity, Vaccines, Synthetic genetics, Vaccinia virus immunology, AIDS Vaccines immunology, HIV Antibodies blood, Vaccines, Synthetic immunology, Vaccinia virus genetics
Abstract

Objective: To investigate the immunogenicity of HIV vaccine vTKgpe based on Vaccinia Virus Tiantan vector in mice and rabbits., Methods: Mice were inoculated with HIV vaccine vTKgpe by intramuscular (i.m.), intradermal (i.d.) or subcutaneous (s.c.) injections. Blood sample were collected every week, and then antibodies against HIV and vaccinia virus vector were detected. At week 4, some mice were killed and cellular immune responses were examined by flow cytometer. Additionally two rabbits were vaccinated subcutaneously, blood sample were tested as done with mice., Results: In mice i.m. and s.c. groups, HIV specific antibodies emerged at week 2 and declined at week 4. Antibodies against vector elevated rapidly at week 4, and potentially affected HIV specific antibody detection. Cellular immune responses were only detected in s.c. group. Serum of rabbit showed that anti-HIV antibody appeared at week 2 and maintained for several weeks., Conclusion: Vaccine vTKgpe innoculated by i.m. and s.c routes inclined to induce humoral immune responses in mice, but in i.d. group, inclined to induce cellular immune responses. Response to the recombinant vaccinia virus was more sensitive in rabbit than in mice.

Published
2004

3. [The construction of attenuated Tiantan recombinant vaccinia virus vector with IFN-gamma receptor gene deletion].

Author

Huang W, Liu Y, Duan DL, Li HS, Liu Y, Hong KX, Zhu JH, and Shao YM

Subjects
Animals, Cell Line, Chick Embryo, Gene Deletion, Humans, Rabbits, Receptors, Interferon genetics, Recombination, Genetic, Vaccines, Attenuated immunology, Vaccinia virus immunology, Virulence, Virus Replication, Interferon gamma Receptor, Genetic Vectors, Receptors, Interferon physiology, Vaccinia virus genetics, Vaccinia virus pathogenicity
Abstract

Objective: B8R gene encodes a secreted protein with homology to IFN-gamma receptor, which neutralizes the antiviral and immunological regulation activities of IFN-gamma. To improve the safety of vaccinia virus vector, an attenuated recombinant vaccinia virus with the B8R gene deletion from Tiantan vaccine strain (VTT) was constructed., Methods: The transfer vectors were generated by joining B8R left flank, B8R right flank, vv promoter, LacZ, multicloning site and pBRSK fragments. The recombinant viruses VTTdeltaB8RLacZ (VTT with B8R deletion and LacZ insertion) were constructed by homologous recombination., Results: The B8R deletion mutants were confirmed by dot blot with B8R gene probe and PCR amplification. The replication ability of VTTdeltaB8RLacZ strain in vitro was similar to that of the VTT. The skin lesions formed by VTTdeltaB8RLacZ (10(6) pfu) were significantly smaller and healed faster than those formed by VTT when injected intradermally to the rabbits,and no visible ulceration occurred. Meanwhile LacZ in VTKgpedeltaB8RLacZ was expressed stably., Conclusions: The attenuated vector with B8R gene deletion improves the safety of recombinant vaccinia virus vaccine B8R locus may be used as a new site for insertion of foreign genes in vaccinia virus vector.

Published
2004

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