58 results on '"Duarte, JMB"'
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2. Cytogenetic research in wild animals at FCAVJ, Brazil. I. Mammals
- Author
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Lui JF, Ribeiro RP, Duarte JMB, Giannoni ML, and Tosta P
- Subjects
Animal culture ,SF1-1100 ,Genetics ,QH426-470 - Published
- 1991
- Full Text
- View/download PDF
3. Cytogenetic research in wild animals at FCAVJ, Brazil. II. Birds
- Author
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Giannoni, ML, Duarte, JMB, Moro, MEG, and Boer, J
- Published
- 1991
- Full Text
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4. Cytogenetic research in wild animals at FCAVJ, Brazil. I. Mammals
- Author
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Giannoni, ML, Duarte, JMB, Ribeiro, RP, Lui, JF, and Tosta, P
- Published
- 1991
- Full Text
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5. Sex identification of South American Parrots (Psittacidae, Aves) using the human minisatellite probe 33.15
- Author
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Miyaki, Cy, Duarte, Jmb, Caparroz, R., Nunes, Alv, Wajntal, A., Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), and PARQUE ZOOL SOROCABA
- Abstract
Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:08:42Z No. of bitstreams: 0 Made available in DSpace on 2014-02-26T17:08:42Z (GMT). No. of bitstreams: 0 Previous issue date: 1997-07-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:19:45Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T15:19:45Z (GMT). No. of bitstreams: 0 Previous issue date: 1997-07-01 UNIV SAO PAULO, DEPT BIOL, BR-05422970 SAO PAULO, SP, BRAZIL UNESP, FCAVJ, DEPT MELHORAMENTO GENET ANIM, BR-01487000 SAO PAULO, SP, BRAZIL PARQUE ZOOL SOROCABA, SOROCABA, SP, BRAZIL UNESP, FCAVJ, DEPT MELHORAMENTO GENET ANIM, BR-01487000 SAO PAULO, SP, BRAZIL
- Published
- 1997
6. Cytotaxonomic analysis of Brazilian species of the genus Amazona (Psittacidae, Aves) and confirmation of the genus Salvatoria (Ribeiro, 1920)
- Author
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Duarte, JMB, Caparroz, R., and Universidade Estadual Paulista (Unesp)
- Abstract
Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:07:02Z No. of bitstreams: 1 WOSA1995TT20400018.pdf: 3033041 bytes, checksum: 83b185c6cc1f5f37e26e6194da633058 (MD5) Made available in DSpace on 2014-02-26T17:07:02Z (GMT). No. of bitstreams: 1 WOSA1995TT20400018.pdf: 3033041 bytes, checksum: 83b185c6cc1f5f37e26e6194da633058 (MD5) Previous issue date: 1995-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:23:51Z No. of bitstreams: 1 WOSA1995TT20400018.pdf: 3033041 bytes, checksum: 83b185c6cc1f5f37e26e6194da633058 (MD5) Made available in DSpace on 2014-05-20T15:23:51Z (GMT). No. of bitstreams: 1 WOSA1995TT20400018.pdf: 3033041 bytes, checksum: 83b185c6cc1f5f37e26e6194da633058 (MD5) Previous issue date: 1995-12-01 The karyotypes of 12 species of Psittacidae of the genus Amazona were studied: A. aestiva, A. amazonica, A. brasiliensis, A. autumnalis, A. farinosa, A, festiva, A. kawalli, A. ochrocephala, A. pretrei, A. rhodocorytha, A. vinacea and A. xanthops. The metaphases were obtained using a short term culture of leather pulp. Eleven of the twelve analyzed species were karyotypically homogeneous, with only a few divergences in chromosomes 2 and 3. The species A. xanthops showed large karyotypic differences compared to the genus Amazona. Consequently, the genus Salvatoria (Ribeiro, Rev. Mus. Paul. 12: 1-82, 1920) was confirmed, and A. xanthops renamed Salvatoria xanthops. The study showed the chromosomic conservation of the genus Amazona and the need for further taxonomic studies of the karyotype of the Psittacidae. UNESP,FAC CIENCIAS AGRARIAS & VET,DEPT MELHORAMENTO GENET ANIM,BR-14870000 JABOTICABAL,SP,BRAZIL UNESP,FAC CIENCIAS AGRARIAS & VET,DEPT MELHORAMENTO GENET ANIM,BR-14870000 JABOTICABAL,SP,BRAZIL
- Published
- 1995
7. CYTOGENETIC ANALYSIS OF THE PAMPAS DEER, OZOTOCEROS-BEZOARTICUS (MAMMALIA, CERVIDAE)
- Author
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Duarte, JMB, Giannoni, M. L., and Universidade Estadual Paulista (Unesp)
- Abstract
Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:06:54Z No. of bitstreams: 1 WOSA1995TA98500021.pdf: 3222928 bytes, checksum: 4b65ac4d17cabc537e6564f6dc32c800 (MD5) Made available in DSpace on 2014-02-26T17:06:54Z (GMT). No. of bitstreams: 1 WOSA1995TA98500021.pdf: 3222928 bytes, checksum: 4b65ac4d17cabc537e6564f6dc32c800 (MD5) Previous issue date: 1995-09-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:29:19Z No. of bitstreams: 1 WOSA1995TA98500021.pdf: 3222928 bytes, checksum: 4b65ac4d17cabc537e6564f6dc32c800 (MD5) Made available in DSpace on 2014-05-20T15:29:19Z (GMT). No. of bitstreams: 1 WOSA1995TA98500021.pdf: 3222928 bytes, checksum: 4b65ac4d17cabc537e6564f6dc32c800 (MD5) Previous issue date: 1995-09-01 The chromosome constitution of five males and three females of the Pampas deer (Ozotoceros bezoarticus) coming mainly from the region of Corumba-MS, was studied. The diploid number of the species was reconfirmed as 68 chromosomes with Fundamental Number (FN) = 74. The X chromosome was the largest and the Y the smallest in the genome. Constitutive heterochromatin demonstrated by C banding was present in the centromeric region of all chromosomes, except in pair number two, which had none, and in chromosome X which had a stained region in the telomere on the long arm, Chromosomes pairs 3 and 4 bore Ag-NORs. The banding patterns differed from those of previous reports for this species. This may be due to subspecific differences. UNESP,FAC CIENCIAS AGR & VET,DEPT MELHORAMENTO GENET ANIM,BR-14870000 JABOTICABAL,SP,BRAZIL UNESP,FAC CIENCIAS AGR & VET,DEPT MELHORAMENTO GENET ANIM,BR-14870000 JABOTICABAL,SP,BRAZIL
- Published
- 1995
8. Genetic diversity of two Brazilian populations of the Pampas deer (Ozotoceros bezoarticus, Linnaeus 1758)
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Rodrigues, FP., primary, Garcia, JF., additional, Ramos, PRR., additional, Bortolozzi, J., additional, and Duarte, JMB., additional
- Published
- 2007
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9. Genetic relatedness among wild, domestic and Brazilian fighting roosters
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Rodrigues, FP, primary, Queiroz, SA, additional, and Duarte, JMB, additional
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- 2006
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10. Genetic diversity of Theileria spp. in deer (Artiodactyla: Cervidae) from Brazil.
- Author
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Calchi AC, Duarte JMB, Castro-Santiago AC, Bassini-Silva R, Barros-Battesti DM, Machado RZ, and André MR
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- Animals, Brazil epidemiology, DNA, Protozoan genetics, Polymerase Chain Reaction veterinary, Sequence Analysis, DNA, HSP70 Heat-Shock Proteins genetics, DNA, Ribosomal genetics, DNA, Ribosomal chemistry, Deer parasitology, Theileria genetics, Theileria isolation & purification, Theileria classification, Genetic Variation, Theileriasis parasitology, Theileriasis epidemiology, RNA, Ribosomal, 18S genetics, Phylogeny
- Abstract
Babesia spp. and Theileria spp. are tick-borne apicomplexan protozoa that can cause disease in animals and humans. Deer are considered reservoirs for a wide variety of Piroplasmida species, including some potentially zoonotic. This study aimed to investigate the occurrence and genetic diversity of piroplasmids in wild deer sampled in four Brazilian states (São Paulo, Mato Grosso do Sul, Paraná and Goiás). For this purpose, extracted DNA samples from 181 deer buffy coat samples (138 Blastocerus dichotomus, 26 Subulo gouazoubira, 4 Mazama jucunda, 3 Mazama rufa and 10 Ozotocerus bezoarticus) were subjected to a nested PCR (nPCR) assay based on the 18S rRNA gene in order to perform a screening for piroplasmids and characterized based on the near-complete 18S rRNA, hsp70 and cox-3 genes. As a result, 75.14% (136/181) samples were positive for piroplasmids. Of these, 108 (79.41%), 101 (74.26%) and 67 (49.26%) were positive to near complete 18S rRNA, hsp70 and cox-3 genes, respectively. Phylogenetic analyses based on three molecular markers showed similar topology to each other. All sequences obtained in the present study were positioned into the Theileria sensu stricto clade, forming a distinct clade, albeit close to T. cervi. Most sequences grouped together into a large clade divided into subclades, which were often related to deer genus/species, showing that Theileria lineages seemed to show specificity according to deer genus/species. Two 18S rRNA sequences (one obtained from S. gouazoubira and another from M. jucunda) were positioned into a different clade, apart from other sequences detected in this study, indicating that different species of Theileria occur in deer from Brazil. Two subclusters were observed in the phylogenetic analysis based on the hsp70 gene: the first containing only sequences detected in marsh deer and the second grouping sequences detected in brocket deer (Mazama spp. and S. gouazoubira). The latter was also divided into smaller clades that grouped Theileria genotypes according to deer species (M. jucunda, M. rufa and S. gouazoubira). This study provides the first molecular evidence of Theileria infection in M. jucunda, as well as co-infection by distinct Theileria (sub)species/genotypes in the same deer was evidenced. Finally, this study expanded the knowledge on the diversity of Theileria spp. infecting deer from South America., Competing Interests: Declarations Ethical approval All animal procedures and management protocols were approved by IBAMA (license number 10636–1) and the Ethics Committee on Animal Use of the School of Agricultural and Veterinarian Scie1nces (FCAV/UNESP) (protocol number 9412/2022). Consent to participate and consent for publication Not applicable. Conflict of interest The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2024
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11. Exposing illegal hunting and wildlife depletion in the world's largest tropical country through social media data.
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El Bizri HR, Oliveira MA, Rampini AP, Knoop S, Fa JE, Coad L, Morcatty TQ, Massocato GF, Desbiez ALJ, Campos-Silva JV, La Laina DZ, Duarte JMB, Barboza RSL, Campos Z, da Silva MB, Mângia S, Ingram DJ, and Bogoni JA
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- Brazil, Animals, Sports legislation & jurisprudence, Humans, Conservation of Natural Resources legislation & jurisprudence, Social Media, Animals, Wild, Hunting
- Abstract
Globally, illegal sport hunting can threaten prey populations when unregulated. Due to its covert nature, illegal sport hunting poses challenges for data collection, hindering efforts to understand the full extent of its impacts. We gathered social media data to analyze patterns of illegal sport hunting and wildlife depletion across Brazil. We collected data for 2 years (2018-2020) across 5 Facebook groups containing posts depicting pictures of illegal sport hunting events of native fauna. We described and mapped these hunting events by detailing the number of hunters involved, the number of species, the mean body mass of individuals, and the number and biomass of individuals hunted per unit area, stratified by Brazilian biome. We also examined the effects of defaunation on hunting yield and composition via regression models, rank-abundance curves, and spatial interpolation. We detected 2046 illegal sport hunting posts portraying the hunting of 4658 animals (∼29 t of undressed meat) across all 27 states and 6 natural biomes of Brazil. Of 157 native species targeted by hunters, 19 are currently threatened with extinction. We estimated that 1414 hunters extracted 3251 kg/million km
2 . Some areas exhibited more pronounced wildlife depletion, in particular the Atlantic Forest and Caatinga biomes. In these areas, there was a shift from large mammals and reptiles to small birds as the main targeted taxa, and biomass extracted per hunting event and mean body mass across all taxonomic groups were lower than in other areas. Our results highlight that illegal sport hunting adds to the pressures of subsistence hunting and the wild meat trade on Brazil's wildlife populations. Enhanced surveillance efforts are needed to reduce illegal sport hunting levels and to develop well-managed sustainable sport hunting programs. These can support wildlife conservation and offer incentives for local communities to oversee designated sport hunting areas., (© 2024 The Author(s). Conservation Biology published by Wiley Periodicals LLC on behalf of Society for Conservation Biology.)- Published
- 2024
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12. Evidence of Natural Zika Virus Infection in Captive Cervid Species in Brazil.
- Author
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Santos FSLD, Oliveira S, Romaldini AHCN, Shimozako HJ, Freire BC, Baldini MHM, Duarte JMB, Zanetti EDS, and Okuda LH
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- Animals, Brazil epidemiology, Female, Male, Animals, Zoo, Deer virology, Zika Virus Infection epidemiology, Zika Virus Infection veterinary, Zika Virus Infection virology, Zika Virus isolation & purification
- Abstract
As part of an epidemiologic study of the Zika virus (ZIKV) in deer (Cervidae), samples from 56 captive deer in south and southeastern Brazil were tested for evidence of ZIKV. Three samples were positive using reverse-transcription quantitative PCR, although no samples were positive by virus isolation., (© Wildlife Disease Association 2024.)
- Published
- 2024
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13. Comparative karyotype analysis of the red brocket deer (M. americana sensu lato and M. rufa) complex: evidence of drastic chromosomal evolution and implications on speciation process.
- Author
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Bernegossi AM, Galindo DJ, Peres PHF, Vozdova M, Cernohorska H, Kubickova S, Kadlcikova D, Rubes J, and Duarte JMB
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- Animals, Chromosomes, Artificial, Bacterial genetics, Chromosome Painting, Deer genetics, Deer classification, Karyotype, Evolution, Molecular, Genetic Speciation, Karyotyping
- Abstract
Chromosomal rearrangements are often associated with playing a role in the speciation process. However, the underlying mechanism that favors the genetic isolation associated with chromosomal changes remains elusive. In this sense, the genus Mazama is recognized by its high level of karyotype diversity among species with similar morphology. A cryptic species complex has been identified within the genus, with the red brocket deer (Mazama americana and Mazama rufa) being the most impressive example. The chromosome variation was clustered in cytotypes with diploid numbers ranging from 42 to 53 and was correlated with geographical location. We conducted an analysis of chromosome evolution of the red brocket deer complex using comparative chromosome painting and Bacterial Artificial Chromosome (BAC) clones among different cytotypes. The aim was to deepen our understanding of the karyotypic relationships within the red brocket, thereby elucidating the significant chromosome variation among closely related species. This underscores the significance of chromosome changes as a key evolutionary process shaping their genomes. The results revealed the presence of three distinct cytogenetic lineages characterized by significant karyotypic divergence, suggesting the existence of efficient post-zygotic barriers. Tandem fusions constitute the main mechanism driving karyotype evolution, following a few centric fusions, inversion X-autosomal fusions. The BAC mapping has improved our comprehension of the karyotypic relationships within the red brocket deer complex, prompting questions regarding the role of these changes in the speciation process. We propose the red brocket as a model group to investigate how chromosomal changes contribute to isolation and explore the implications of these changes in taxonomy and conservation., (© 2024. The Author(s), under exclusive licence to Institute of Plant Genetics Polish Academy of Sciences.)
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- 2024
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14. Cryopreservation of spermatozoa from black-and-gold howler monkeys (Alouatta caraya) using egg yolk-based or soy lecithin-based extenders.
- Author
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Burch FC, Nichi M, Mendes CM, Assumpção MEOD, Duarte JMB, and Del Rio do Valle R
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- Animals, Male, Lecithins, Glycine max chemistry, Glycerol, Sperm Motility drug effects, Cryopreservation veterinary, Cryopreservation methods, Semen Preservation veterinary, Semen Preservation methods, Cryoprotective Agents, Egg Yolk chemistry, Spermatozoa physiology, Alouatta physiology
- Abstract
There are more than 200 species and subspecies of Neotropical Primates of which more than 40% are listed as threatened by the IUCN Red List of Threatened Species. Both in situ and ex situ conservation programs can benefit from the use of assisted reproductive technologies. The objective of this study was to evaluate, for the first time, cryopreservation techniques for Alouatta caraya semen. Semen samples were collected from five adult males, analyzed, and frozen in either Test-egg yolk or Test-soy lecithin-based extenders containing either 3 or 4% glycerol. Frozen-thawed samples were analyzed at 10, 40, and 80 min post-thaw. Egg yolk-based extenders were overall better than soy lecithin-based extenders. There was no significant difference between 3 and 4% glycerol in any of the parameters analyzed, however, 4% glycerol in egg yolk-based extender produced more favorable results for total motility, intact plasma membrane, lipid peroxidation, and DNA fragmentation index. This study brought novel information on semen characteristics and cryopreservation aspects for A. caraya, which can help shape future experiments to improve the outcome of frozen-thawed sperm for this and other species of Neotropical primates., (© 2024. The Author(s), under exclusive licence to Japan Monkey Centre.)
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- 2024
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15. Prevalence and Risk Factors of Bone and Dental Lesions in Neotropical Deer.
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Silva TA, Martins ADS, Alves LR, Pereira LWB, Saraiva JR, Duarte JMB, Zanetti EDS, Schweitzer CM, Dutra IS, and Borsanelli AC
- Abstract
Bone and dental lesions have been documented in various deer species globally, affecting the efficiency of ingestion and digestion, consequently influencing their general health and leading to a decline in survival and reproductive performance. The present study aimed to characterize bone and dental lesions in the dry skulls of individual deer, estimate the prevalence of these lesions, and assess potential risk factors associated with the development of bone and dental alterations. This study assessed bone and dental lesions in 180 dry skulls of eleven neotropical deer species, originating from both captivity and wildlife conditions, through direct visual inspection. A high prevalence of bone and dental lesions was observed in all analyzed species. Dental calculus was the most common alteration (96.7%), followed by dental wear (71.1%). Animal age positively correlated with most bone and dental alterations, indicating that older animals showed more lesions. Additionally, the prevalence of these alterations was similar between sexes. Moreover, all lesions were more common in captive-bred animals, likely attributed to their older age and a less diverse diet. Blastocerus dichotomus and Mazama americana were most affected by bone resorption and dental trauma and had the highest dental calculus prevalence, along with Subulo gouazoubira and Passalites nemorivagus . All eleven species evaluated in the present study were susceptible to the occurrence of bone and dental lesions. Therefore, monitoring oral health and diet in captivity are fundamental practices for the conservation of these species.
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- 2024
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16. Transcervical artificial insemination in the brown brocket deer (Subulo gouazoubira): a promising method for assisted reproduction in deer.
- Author
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Duarte GS, Galindo DJ, Baldini MHM, da Fonseca JF, Duarte JMB, and Oliveira MEF
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- Animals, Female, Male, Cervix Uteri, Insemination, Artificial veterinary, Insemination, Artificial methods, Oxytocin, Uterus, Cross-Over Studies, Deer, Semen Preservation veterinary
- Abstract
The present study aimed to test the efficiency of transcervical artificial insemination techniques with cervical immobilization (TCAI-CI) or cervical traction (TCAI-CT), associated or not with the use of oxytocin (OT) as a protocol for cervical dilation, in the brown brocket deer (Subulo gouazoubira). The study was carried out in a crossover design using four adult females in two replicates with an interval of 60 days. Estrus was synchronized with oral melengestrol acetate (MGA) associated with estradiol benzoate and sodium cloprostenol. TCAI techniques were performed from 18 to 24 h after estrus onset. All females received either an i.v. application of 50 IU of OT (G-OT, n = 4) or 1 mL of saline solution (G-Control, n = 4) 20 min before the TCAI procedure. The TCAIs were performed using frozen-thawed semen motility 40%, vigor 3, acrosome integrity 87%, membrane integrity of 95% and 13% of total post-thaw defects from the same batch. Behavioral estrus was observed in 100% of the females, in both replicates. It was achieved a 50% (4/8) success of cervical transposition with semen deposition in the uterine. Regarding inseminations, most of them (87.5%) were performed using the TCAI-CT technique, and the overall conception rate was 50%. Cervical transposition times (< 1 min) and TCAI procedures (~ 17 min) were considered satisfactory. Thus, the performance of the TCAI-CI and TCAI-CT techniques was successful, regardless of using OT as a cervical dilation protocol. This procedure is proposed as a method of choice for artificial insemination with greater applicability in different conservation centers, compared to more advanced reproductive biotechniques, and with a favorable impact on the conservation of deer species., (© 2023. Springer Nature Limited.)
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- 2023
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17. Evaluation of minimally invasive estrus synchronization protocols in brown brocket deer (Subulo gouazoubira).
- Author
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Ferrari B, Galindo DJ, Gimenes LU, and Duarte JMB
- Subjects
- Female, Animals, Progesterone pharmacology, Estrus Synchronization methods, Estrus, Estradiol pharmacology, Dinoprost pharmacology, Insemination, Artificial veterinary, Deer, Melengestrol Acetate
- Abstract
This study aimed to evaluate minimally invasive protocols for estrus synchronization in the brown brocket deer (Subulo gouazoubira). Females were submitted to Latin square design, in different treatments. All females received 0.25 mg of estradiol benzoate on the first day of treatment, concomitant with one of the following sources of progesterone: (1) DIP: an intravaginal progesterone releasing device for eight days, (2) MGA1x: once a day (in the morning) oral dose of 1 mg melengestrol acetate for eight days, (3) MGA2x: twice a day (morning and afternoon) oral doses of 0.5 mg of MGA for eight days, (4) P4LA: a single i.m. administration of 75 mg of long-acting progesterone (P4LA). Eight days after the beginning of each treatment, females received an i.m. administration of 265 µg of prostaglandin (PGF
2α ; cloprostenol). Treatment efficacy was evaluated by manifestation of behavioral estrus after treatment and concentration of fecal progesterone metabolites (FPM). The time to onset of estrus in treatment P4LA was significantly longer (180 ± 38.9 h) compared to DIP (63 ± 6.6 h), MGA1x (53 ± 14.4 h) and MGA2x (41 ± 10.1 h) (P = 0.008). According to individual baseline FPM and FPM concentration during the days after estrus, the corpus luteum formation was suggested in all females which responded to the treatments (93.75 %). Low synchrony, longer interval between PGF2α administration and onset of estrus suggest that the P4LA dose (75 mg) is too high and not effective for S. gouazoubira. DIP, MGA 1x and MGA 2x, were effective in estrus synchronization., Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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18. Assessing the Taxonomic Status of the Gray Brocket Mazama simplicicornis argentina Lönnberg, 1919 (Artiodactyla: Cervidae).
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Sandoval EDP, Vacari GQ, Juliá JP, González S, Vozdova M, Cernohorska H, Kubickova S, Kalthoff DC, and Duarte JMB
- Abstract
Mazama simplicicornis argentina is the name that was given to describe a gray brocket collected by Lönberg in 1919 in the central Chaco region of Argentina. Subsequent authors, based on morphological similarities, considered this name to be a synonym for the species Subulo gouazoubira Fischer, 1814 from Paraguay. In the absence of genetic analyses to compare the Argentinian and Paraguayan gray brockets, we aimed to clarify the taxonomy of M. simplicicornis argentina through an integrative assessment using morphological, cytogenetical, and molecular data from its holotype and a current topotype. Qualitative skull features and cranio-morphometric results of M. simplicicornis argentina showed a great similarity with the S. gouazoubira neotype characters. The diploid chromosome number of M. simplicicornis argentina topotype corresponded with the karyotypical pattern of S. gouazoubira with 2n = 70 and FN = 70, showing a great similarity in all classic and molecular cytogenetic results and revealing the homologies between karyotypes. The phylogenetic analysis of mitochondrial genes used in this study (concatenated partial ND5 and Cytb gene) allocated the M. simplicicornis argentina specimens in the monophyletic clade of S. gouazoubira with a branch value of 100%. These results show that there is no discontinuity between the Argentinian and Paraguayan gray brockets. Therefore, the individuals originally described as M. simplicicornis argentina should be recognized as S. gouazoubira .
- Published
- 2023
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19. Effect of dietary fiber on fecal androgens levels: An experimental analysis in brown brocket deer (Mazama gouazoubira).
- Author
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de Souza LJ, Tanaka Y, Di Santo LG, and Duarte JMB
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- Animals, Male, Androgens metabolism, Dietary Fiber metabolism, Feces, Deer metabolism
- Abstract
Non-invasive endocrinology is an important tool for animal conservation, but its success depends on many factors (e.g. adequate hormonal extraction, diet, antibody used in the assay). Dietary fiber is one of the main sources that can lead to erroneous interpretation of the endocrine status provided by EIA analysis. This study aimed to evaluate the dietary fiber effect on the fecal androgen metabolites (FAM) detection, on the daily defecation rate and fecal production, as well as to analyze the gastrointestinal passage and retention time of the experimental diets. Eight brown brocket deer (Mazama gouazoubira) males were randomly assigned to two groups and submitted to both isocaloric and isoproteic experimental diets for 10 days, in a crossover system: low fiber percentage feed (LF, 7% fiber) and high fiber percentage feed (HF, 19% fiber). Such groups were alternated in the middle of this period, with an interval of 10 days between them. In addition, there was a five-day adaptation phase at the beginning of each diet. Fecal collection for FAM measurement was performed during 10 days of treatment, whereas,the defecation rate and fecal production were performed every two hours, for 6 days. The mean FAM level in the HF group was 5038.0 ± 1529.1 ng/g, while for LF, 2178.7 ± 824.9 ng/g (p < 0.05). The mean HF fecal production was 182.6 ± 36.2 g DM/day and 117.5 ± 12.6 g DM/day for LF (p < 0.05). There were no differences in terms of mean defecation rate, passage, and retention times between groups. The results suggest that dietary fiber affects the FAM detection, and this should be taken into consideration before conducting experiments using fecal samples as a source of reproductive hormones profiling., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
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20. Assessment of transplacental transmission of Neospora caninum and Toxoplasma gondii in Neotropical deer: An estimative based on serology.
- Author
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Baldini MHM, Sandoval EDP, and Duarte JMB
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- Animals, Antibodies, Protozoan, Brazil epidemiology, Seroepidemiologic Studies, Coccidiosis parasitology, Coccidiosis veterinary, Deer parasitology, Neospora, Toxoplasma, Toxoplasmosis, Animal parasitology
- Abstract
Transplacental transmission of Neospora caninum and Toxoplasma gondii is well known in some domestic species and despite both parasites having been proved to infect deer fetuses during gestation, the congenital transmission rate in South-American deer species is still unknown. This study aimed to estimate the congenital transmission rate of neosporosis and toxoplasmosis in captive deer populations from Brazil, through serological techniques (IFAT and ELISA). Serum samples from 82 deer were tested by both techniques 20.73 % (IFAT) to 25.60 % (ELISA) were seropositive for T. gondii; the kappa test showed an index of 0.277 of agreement between both techniques. For N. caninum, 40.24 % (IFAT) to 39.02 % (ELISA) were seropositive, with an index of 0.833 of agreement between techniques. Family trees for each species were drawn and we estimated the congenital transmission rate of the diseases. We found a rate of 81.25 % congenital transmission of N. caninum and no evidence of congenital transmission of T. gondii. Vertical transmission appears to be the main route of introduction and maintenance of N. caninum in these captive deer populations, while T. gondii seems to be favored by the horizontal route., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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21. Cytogenetic Mapping of Cattle BAC Probes for the Hypothetical Ancestral Karyotype of the Family Cervidae.
- Author
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Bernegossi AM, Vozdova M, Cernohorska H, Kubickova S, Galindo DJ, Kadlcikova D, Rubes J, and Duarte JMB
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- Animals, Cattle genetics, Chromosomes, Artificial, Bacterial genetics, In Situ Hybridization, Fluorescence methods, Karyotype, Karyotyping, X Chromosome, Deer genetics
- Abstract
Cervids are characterized by their greatest karyotypic diversity among mammals. A great diversity of chromosome numbers in notably similar morphological groups leads to the existence of several complexes of cryptic species and taxonomic uncertainties. Some deer lineages, such as those of Neotropical deer, stand out for a rapid chromosomal reorganization and intraspecific chromosome polymorphisms, which have not been properly explored yet. For that reason, we contribute to the study of deer karyotype diversity and taxonomy by producing and characterizing new molecular cytogenetic markers for the gray brocket deer (Subulo gouazoubira), a deer species that retained the hypothetical ancestral karyotype of Cervidae. We used bacterial artificial chromosome (BAC) clones derived from the cattle genome (Bos taurus) as markers, which were hybridized on S. gouazoubira metaphase chromosomes. In total, we mapped 108 markers, encompassing all gray brocket deer chromosomes, except the Y chromosome. The detailed analysis of fluorescent in situ hybridization results showed 6 fissions and 1 fusion as interchromosomal rearrangements that have separated cattle and gray brocket deer karyotypes. Each group of BAC probes derived from bovine chromosome pairs 1, 2, 5, 6, 8, and 9 showed hybridization signals on 2 different chromosomes, while pairs 28 and 26 are fused in tandem in a single acrocentric chromosome in S. gouazoubira. Furthermore, the BAC markers detected the occurrence of intrachromosomal rearrangements in the S. gouazoubira chromosomes homologous to pair 1 and the X chromosome of cattle. We present a karyotypic map of the 108 new markers, which will be of great importance for future karyotypic evolution studies in cervids and, consequently, help in their conservation and taxonomy resolution., (© 2022 S. Karger AG, Basel.)
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- 2022
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22. Revalidation of Mazama rufa (Illiger 1815) (Artiodactyla: Cervidae) as a Distinct Species out of the Complex Mazama americana (Erxleben 1777).
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Peres PHF, Luduvério DJ, Bernegossi AM, Galindo DJ, Nascimento GB, Oliveira ML, Sandoval EDP, Vozdova M, Kubickova S, Cernohorska H, and Duarte JMB
- Abstract
The red brocket deer Mazama americana Erxleben, 1777 is considered a polyphyletic complex of cryptic species with wide chromosomal divergence. Evidence indicates that the observed chromosomal divergences result in reproductive isolation. The description of a neotype for M. americana allowed its genetic characterization and represented a comparative basis to resolve the taxonomic uncertainties of the group. Thus, we designated a neotype for the synonym Mazama rufa Illiger, 1815 and tested its recognition as a distinct species from the M. americana complex with the analysis of morphological, cytogenetic and molecular data. We also evaluated its distribution by sampling fecal DNA in the wild. Morphological data from craniometry and body biometry indicated an overlap of quantitative measurements between M. rufa and the entire M. americana complex. The phylogenetic hypothesis obtained through mtDNA confirmed the reciprocal monophyly relationship between M. americana and M. rufa , and both were identified as distinct molecular operational taxonomic units by the General Mixed Yule Coalescent species delimitation analysis. Finally, classic cytogenetic data and fluorescence in situ hybridization with whole chromosome painting probes showed M. rufa with a karyotype of 2n = 52, FN = 56. Comparative analysis indicate that at least fifteen rearrangements separate M. rufa and M. americana ( sensu stricto ) karyotypes, which confirmed their substantial chromosomal divergence. This divergence should represent an important reproductive barrier and allow its characterization as a distinct and valid species. Genetic analysis of fecal samples demonstrated a wide distribution of M. rufa in the South American continent through the Atlantic Forest, Cerrado and south region of Amazon. Thus, we conclude for the revalidation of M. rufa as a distinct species under the concept of biological isolation, with its karyotype as the main diagnostic character. The present work serves as a basis for the taxonomic review of the M. americana complex, which should be mainly based on cytogenetic characterization and directed towards a better sampling of the Amazon region, the evaluation of available names in the species synonymy and a multi-locus phylogenetic analysis., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Peres, Luduvério, Bernegossi, Galindo, Nascimento, Oliveira, Sandoval, Vozdova, Kubickova, Cernohorska and Duarte.)
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- 2021
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23. Cytochrome b sequence of the Mazama americana jucunda Thomas, 1913 holotype reveals Mazama bororo Duarte, 1996 as its junior synonym.
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Mantellatto AMB, González S, and Duarte JMB
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The small red brocket deer, Mazama bororo Duarte, 1996 was described based on karyotypical and morphological characters. However, the original description of Mazama americana jucunda suggested that this subspecies could represent the same taxon as Mazama bororo. This assumption was based on the type locality of Mazama americana jucunda and on morphological similarities between Mazama americana jucunda and Mazama bororo. To solve this question, we obtained DNA sequences of the holotype of Mazama americana jucunda and compared it with other species of Mazama, including the holotype of M. bororo. A phylogenetic tree was obtained to verify the relationships among these taxa. The results clearly showed that M. americana jucunda and M. bororo represent the same biological entity. Therefore, the oldest name available for the small red brocket occurring in the Atlantic Forest of southern Brazil should be Mazama jucunda, remaining M. bororo as a junior synonym. We emphasise the importance of using DNA from museum specimens, especially from holotypes, in order to obtain a more accurate taxonomic identification. We also highlight the importance of application of valid names for labelling all aspects of biodiversity research, as well as for monitoring and conservation efforts.
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- 2021
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24. Evaluation of potential reproductive seasonality in brown brocket deer (Mazama gouazoubira) bucks.
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Tanaka Y, Herédias-Ribas CM, Baldini MHM, Guevara JEH, and Duarte JMB
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- Animals, Male, Reproduction, Sperm Motility, Testis, Deer, Semen Analysis veterinary
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Photoperiod is the main cue that controls seasonal reproduction in deer from temperate regions, like secretion of testosterone by the testis, neck enlargement, and testis development. However, little is known about its effect on the reproduction of tropical deer species. This research aimed to assess the potential seasonality of the brown brocket deer (Mazama gouazoubira) by melatonin treatment. Reproductive parameters such as testicular volume, neck circumference, and semen quality were evaluated from day 0 to 90, and fecal androgen metabolites (FAM) from day -28 to 91. Melatonin treatment was administered from day 0 to 60. In total, seven individuals were used in the study. Four animals were allocated to the treated group (TG) and three to the control group (CG). The neck circumference and some seminal parameters did not show a significant difference between CG and TG and along the experimental period. High FAM levels were observed for the TG on days 7, 14, 21, 35, 84, and 91 (p < 0.05). The mean testicular volume was reduced from day 0 to 30 (p < 0.05) and from day 0 to 90 (p < 0.05), with no increase in the mean testicular volume from day 30 to 60 (p > 0.05). Sperm motility was the highest on day 60 compared with day 0 (p < 0.05). Although not statistically different, membrane integrity and major defects tended to be higher and lower, respectively, in the TG compared with the CG. For these parameters, the incremental change (%) per individual showed that males from the TG tended to have a higher percentage during the melatonin treatment, hence melatonin might affect these seminal characteristics. These findings suggest that exogenous melatonin might enhance FAM levels and sperm motility., Competing Interests: Declaration of competing interest None., (Copyright © 2021 Elsevier Inc. All rights reserved.)
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- 2021
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25. Sperm chromosome segregation of rob(4;16) and rob(4;16)inv(4) in the brown brocket deer (Mazama gouazoubira).
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Galindo DJ, Vozdova M, Kubickova S, Cernohorska H, Bernegossi AM, Kadlcikova D, Rubes J, and Duarte JMB
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- Animals, Cattle, Chromosome Segregation, Karyotyping veterinary, Male, Spermatozoa, Translocation, Genetic, Cattle Diseases, Deer genetics
- Abstract
The genus Mazama stands out among the Neotropical deer due to their wide intra and interspecific karyotypic diversification, which is associated with an accentuated chromosomal fragility. There are reports of heterozygous Robertsonian translocation (RT) carriers in a free-range population of Mazama gouazoubira (brown brocket deer), as well as in captive animals of this and other species of the genus. To analyze possible negative impacts of heterozygous chromosome rearrangements on reproductive fitness of the carriers, we performed an analysis of sperm meiotic segregation in four brown brocket bucks, carriers of a rob(4;16), and compared the results with those of a normal buck. We established a reliable FISH and sperm-FISH protocol for the brown brocket deer using bovine (Bos taurus; diploid number, 2n = 60) whole chromosome painting (WCP) and BAC probes. Using BAC probes, we revealed the presence of a paracentric inversion (PAI) of the fused chromosome 4 in two of the four analyzed RT carriers. The mean frequency of normal/balanced sperm in the translocation carriers was significantly lower than in the normal buck (94.78% vs 98.40%). The mean value of total unbalanced spermatozoa was almost doubled in the RT/PAI carriers (6.68%) when compared to RT carriers (3.76%), but the difference was not statistically significant. This study demonstrated the efficiency of FISH with bovine WCP and BAC probes in the characterization of chromosome rearrangements and gametic segregation patterns in brown brocket deer. Our results indicate a low to moderate increase in the rates of unbalanced meiotic segregation products in brown brocket bucks heterozygous for RT and RT/PAIs., Competing Interests: Declaration of competing interest The authors declare no conflict of interest to disclose., (Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)
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- 2021
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26. Assisted Reproductive Technology in Neotropical Deer: A Model Approach to Preserving Genetic Diversity.
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Rola LD, Buzanskas ME, Melo LM, Chaves MS, Freitas VJF, and Duarte JMB
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One of the most significant challenges in deer is the ability to maintain genetic diversity, avoiding inbreeding and sustaining population health and reproduction. Although our general knowledge of reproductive physiology is improving, it appears that the application of assisted reproductive technology (ART) will more efficiently advance wildlife conservation efforts and preserve genetic diversity. The purpose of this review is to present the most important results obtained with the use of ART in Neotropical deer. Thus, the state-of-the-art for estrus synchronization, semen technology, artificial insemination, and in vivo embryo production will be presented. In vitro embryo production (IVP) is also a biotechnology that is taking initial steps in deer. In this aspect, the approach with the proteomics of ovarian follicular fluid is being used as a tool for a better understanding of oocyte maturation. Finally, cell banks and the use of interspecific somatic cell nuclear transfer (iSCNT) as well as the use of stem cells for gametes differentiation are promising techniques.
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- 2021
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27. Collection and in vitro maturation of Mazama gouazoubira (brown brocket deer) oocytes obtained after ovarian stimulation.
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Rola LD, Zanetti EDS, Del Collado M, Peroni EFC, and Duarte JMB
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- Animals, Female, Fertilization in Vitro, Follicle Stimulating Hormone, Ovarian Follicle, Ovulation Induction, Deer, Oocytes
- Abstract
In vitro production of embryos has gained prominence as a tool for use in wildlife conservation programmes in situ and ex situ. However, the development of this technique depends on steps that include ovarian stimulation, collection and oocyte maturation. The purpose of this study was to assess the feasibility of an ovarian stimulation protocol for follicular aspiration, the efficiency of videolaparoscopy for follicular aspiration and test a medium for in vitro oocyte maturation for the species Mazama gouazoubira. Five females were submitted to repeated ovarian stimulation (hormone protocol using controlled internal drug release), and estradiol benzoate on D0 and eight injections of follicle-stimulating hormone, once every 12 h, from D4 onwards at 30-day intervals. Fourteen surgical procedures were performed in superstimulated females, resulting in the collection of 94 oocytes and an average of 17.1 ± 9.1 follicles observed, 13.5 ± 6.6 follicles aspirated and 7.2 ± 3.7 oocytes collected per surgery. After collection, the oocytes were submitted to in vitro maturation for 24 h and stained with Hoechst 33342 dye to evaluate their nuclear status; 64.5% of the oocytes reached MII and 16.1% were spontaneously activated by parthenogenesis. The nuclear status of oocytes that did not undergo in vitro maturation was evaluated; 80.9% were found to be immature.
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- 2021
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28. Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids.
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Teixeira WFP, De Oliveira ML, de Faria Peres PH, Nagata WB, Santana BN, Oliveira BCM, Duarte JMB, Cardoso TC, Lopes WDZ, and Bresciani KDS
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- Animals, Brazil, Cattle, Cryptosporidiosis parasitology, Polymerase Chain Reaction veterinary, Polymorphism, Restriction Fragment Length, RNA, Helminth analysis, RNA, Ribosomal, 18S analysis, Cryptosporidiosis diagnosis, Cryptosporidium parvum isolation & purification, Deer, Feces parasitology
- Abstract
This research had as objective to evaluate the occurrence and to characterize genetically the infections by Cryptosporidium in Mazama gouazoubira. By a non-invasive harvest methodology using trained sniffer dogs to locate fecal samples of cervids, 642 fecal samples were obtained from six Brazilian localities. The cervids species responsible for the excretion of each fecal sample were identified by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), using the mitochondrial cytochrome b target gene (cyst b) and the restriction enzymes Sspl, AflIII and BstN. From this identification, 437 fecal samples of M. gouazoubira were selected for research of Cryptosporidium spp. performed through negative staining with malachite green and polymerase chain reaction (nPCR), with the subunit of 18S rRNA gene, followed by sequencing the amplified products. In the samples that were diagnosed the presence of parasite species with zoonotic potential, genotyping was also performed using nPCR with the subunit of GP60 gene. Statistical analysis consisted of the Fisher exact test to verify the association of the presence of the enteroparasite in relation to the presence of cattle in each locality, and the McNemar tests and Kappa correlation coefficient used to compare the results obtained between the two diagnostic techniques. In the fecal samples of M. gouazoubira the occurrences of Cryptosporidium were diagnosed in 1.6% (7/437) and 1.1% (5/437), respectively, through nPCR and microscopy. Cryptosporidium. parvum was diagnosed in 100% (7/7) of the samples submitted to sequencing (18S gene). The IIaA16G3R1 subtype was diagnosed in five of the C. parvum samples submitted to genotyping (GP60 gene). This is the first world report of C. parvum in M. gouazoubira and subtype IIaA16G3R1 in cervids., (Copyright © 2020. Published by Elsevier B.V.)
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- 2021
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29. Chromosomal Polymorphism and Speciation: The Case of the Genus Mazama (Cetartiodactyla; Cervidae).
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Galindo DJ, Martins GS, Vozdova M, Cernohorska H, Kubickova S, Bernegossi AM, Kadlcikova D, Rubes J, and Duarte JMB
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- Animals, Breeding, Chromosome Painting, Female, Hybridization, Genetic, In Situ Hybridization, Fluorescence, Male, Chromosomes, Polymorphism, Genetic, Ruminants genetics
- Abstract
Chromosomal polymorphism plays a major role in speciation processes in mammals with high rates of karyotypic evolution, as observed in the family Cervidae. One remarkable example is the genus Mazama that comprises wide inter- and intra-specific chromosomal variability. To evaluate the impact of chromosomal polymorphisms as reproductive barriers within the genus Mazama , inter-specific hybrids between Mazama gouazoubira and Mazama nemorivaga (MGO × MNE) and intra-specific hybrids between cytotypes of Mazama americana (MAM) differing by a tandem (TF) or centric fusion (Robertsonian translocations-RT) were evaluated. MGO × MNE hybrid fertility was evaluated by the seminal quality and testicular histology. MAM hybrids estimation of the meiotic segregation products was performed by sperm-FISH analysis. MGO × MNE hybrids analyses showed different degrees of fertility reduction, from severe subfertility to complete sterility. Regarding MAM, RT, and TF carriers showed a mean value for alternate segregation rate of 97.74%, and 67.23%, and adjacent segregation rate of 1.80%, and 29.07%, respectively. Our results suggested an efficient post-zygotic barrier represented by severe fertility reduction for MGO × MNE and MAM with heterozygous TF. Nevertheless, RT did not show a severe effect on the reproductive fitness in MAM. Our data support the validity of MGO and MNE as different species and reveals cryptic species within MAM.
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- 2021
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30. Temperament, Plasticity, and Emotions in Defensive Behaviour of Paca (Mammalia, Hystricognatha).
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Nogueira SSC, Nogueira-Filho SLG, Duarte JMB, and Mendl M
- Abstract
Within a species, some individuals are better able to cope with threatening environments than others. Paca ( Cuniculus paca ) appear resilient to over-hunting by humans, which may be related to the behavioural plasticity shown by this species. To investigate this, we submitted captive pacas to temperament tests designed to assess individual responses to short challenges and judgement bias tests (JBT) to evaluate individuals' affective states. Results indicated across-time and context stability in closely correlated "agitated", "fearful" and "tense" responses; this temperament dimension was labelled "restless". Individual "restless" scores predicted responses to novelty, although not to simulated chasing and capture by humans in a separate modified defence test battery (MDTB). Restless animals were more likely to show a greater proportion of positive responses to an ambiguous cue during JBT after the MDTB. Plasticity in defensive behaviour was inferred from changes in behavioural responses and apparently rapid adaptation to challenge in the different phases of the MDTB. The results indicate that both temperament and behavioural plasticity may play a role in influencing paca responses to risky situations. Therefore, our study highlights the importance of understanding the role of individual temperament traits and behavioural plasticity in order to better interpret the animals' conservation status and vulnerabilities.
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- 2021
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31. Low invasive estrous synchronization protocol for wild animals: an example with melengestrol acetate in brown brocket deer (Mazama gouazoubira) .
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Tanaka Y, Americano AP, Galindo DJ, and Duarte JMB
- Abstract
Deer are sensitive to stressful stimuli by handling and their reproductive physiology could be altered by these procedures, making it necessary to develop less invasive protocols for ART. Melengestrol acetate (MGA), a synthetic progestin administered orally, appears as an alternative for estrous synchronization protocols (ESP), such as reported in cattle. Firstly, we compared two MGA doses (0.5 and 1.0 mg/day/animal), which would have suppression effect in estrous behavior (EB). Eight females were randomly and equally distributed in Group 1 (G1) and Group 2 (G2), which received 0.5 and 1.0 mg/day/animal respectively for 15 days (D1 to D15). Two cloprostenol (CP) applications were performed on D0 and D11. Estrus detection (ED) was performed every day. All females from G1 displayed estrus during treatment period, whereas all females from G2 displayed estrus after treatment, suggesting a suppressive effect of 1.0 mg in the EB. Once the suppressive MGA dose (1.0 mg) was defined, we used this dose for assessing ESP. The same eight females received 1.0 mg/animal for eight days (D-8 to D-1), followed by 0.25 mg of estradiol benzoate on D-8 and 265 μg of CP on D0. Feces for fecal progesterone metabolites (FPM) measurement were collected from D0 until seven days after the last day of estrus. Seven females displayed estrus between 12 and 72 h after CP application, which was followed by a significant increase in FPM levels (except female MG6), suggesting the formation of corpus luteum. After ED, females were placed with a fertile male to assess the fertility of the protocol. Pregnancy was confirmed by ultrasound 30 days after mating in 3/6 individuals. Although the low effectiveness of MGA protocol, it should be considered as a promising alternative in deer ESP since this protocol has less stressful effect on the animal during reproductive management when compared to other ESP., Competing Interests: Conflict of interest: The authors have no conflict of interest to declare., (Copyright © The Author(s).)
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- 2021
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32. NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics.
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Nagy-Reis M, Oshima JEF, Kanda CZ, Palmeira FBL, de Melo FR, Morato RG, Bonjorne L, Magioli M, Leuchtenberger C, Rohe F, Lemos FG, Martello F, Alves-Eigenheer M, da Silva RA, Silveira Dos Santos J, Priante CF, Bernardo R, Rogeri P, Assis JC, Gaspar LP, Tonetti VR, Trinca CT, Ribeiro AS, Bocchiglieri A, Hass A, Canteri A, Chiarello AG, Paglia AP, Pereira AA, de Souza AC, Gatica A, Medeiro AZ, Eriksson A, Costa AN, González-Gallina A, Yanosky AA, Jesus de la Cruz A, Bertassoni A, Bager A, Bovo AAA, Cravino Mol A, Bezerra AMR, Percequillo A, Vogliotti A, Costa Lopes AM, Keuroghlian A, Zúñiga Hartley AC, Devlin AL, de Paula A, García-Olaechea A, Sánchez A, Aquino ACMM, Srbek-Araujo AC, Ochoa AC, Tomazzoni AC, Lacerda ACR, Bacellar AEF, Campelo AKN, Herrera Victoria AM, Paschoal AMO, Potrich AP, Gomes APN, Olímpio APM, Cunha Costa AR, Jácomo ATA, Calaça AM, Jesus AS, de Barros Barban A, Feijó A, Pagoto A, Rolim AC, Hermann AP, Souza ASMCE, Chein Alonso A, Monteiro A, Mendonça AF, Luza AL, Moura ALB, da Silva ALF, Lanna AM, Antunes AP, Nunes AV, Dechner A, Carvalho AS, Novaro AJ, Scabin AB, Gatti A, Nobre AB, Montanarin A, Deffaci ÂC, de Albuquerque ACF, Mangione AM, Pinto AMS, Mendes Pontes AR, Bertoldi AT, Calouro AM, Fernandes A, Ferreira AN, Ferreguetti AC, Rosa ALM, Banhos A, Francisco BDSS, Cezila BA, Beisiegel BM, de Thoisy B, Ingberman B, Neves BDS, Pereira-Silva B, Bertagni de Camargo B, Andrade BDS, Santos BS, Leles B, Torres Parahyba Campos BA, Kubiak BB, França BRA, Saranholi BH, Pereira Mendes C, Cantagallo Devids C, Pianca C, Rodrigues C, Islas CA, de Lima CA, de Lima CR, Gestich CC, Tedesco CD, De Angelo C, Fonseca C, Hass C, Peres CA, Kasper CB, Durigan CC, Fragoso CE, Verona CE, Rocha CFD, Salvador CH, Vieira CL, Ruiz CEB, Cheida CC, Sartor CC, Espinosa CDC, Fieker CZ, Braga C, Sánchez-Lalinde C, Machado CIC, Cronemberger C, Luna CL, Del Vechio C, Bernardo CSS, Hurtado CM, Lopes CM, da Rosa CA, Cinta CC, Costa CG, Zárate-Castañeda CP, Novaes CL, Jenkins CN, Seixas CS, Martin C, Zaniratto CP, López-Fuerte CF, da Cunha CJ, De-Carvalho CB, Chávez C, Santos CC, Polli DJ, Buscariol D, Carreira DC, Galiano D, Thornton D, Ferraz DDS, Lamattina D, Moreno DJ, Moreira DO, Farias DA, Barros-Battesti DM, Tavares DC, Costa Braga D, Gaspar DA, Friedeberg D, Astúa D, Silva DA, Viana DC, Lizcano DJ, Varela DM, Loretto D, Gräbin DM, Eaton DP, Machado da Silva D, Dias DM, Camara EMVC, Barbier E, Chávez-González E, Rocha EC, Lima ES, Carrano E, Eizirik E, Nakano-Oliveira E, Rigacci ED, Santos EM, Venticinque EM, Alexandrino ER, Abreu Ribeiro E, Setz E, Rocha ECLD, Carvalho EAR Jr, Rechenberg E, Fraga EDC, Mendonça EN, D'Bastiani E, Isasi-Catalá E, Guijosa-Guadarrama E, Ramalho EE, González E, Hasui É, Saito EN, Fischer E, Aguiar EF, Rocha ES, Martínez Nambo ED, de la Peña-Cuéllar E, Castro ÉP, de Freitas EB, Pedó E, Rocha FL, Girardi F, Pereira FA, Soares FAM, Roque FO, Díaz-Santos FG, Patiu FM, do Nascimento FO, Keesen Ferreira F, Diaz-Santos F, Moreli Fantacini F, Pedrosa F, Pessoa da Silva F, Velez-Garcia F, Gomes FBR, Guedes da Silva F, Michalski F, de Azevedo FC, de Barros FC, Santos FDS, Abra FD, Ramalho FDP, Hatano FM, Anaguano-Yancha F, Gonçalves F, Pedroni F, Passos FC, Jacinavicius FC, Bonfim FCG, Puertas FH, Contreras-Moreno FM, Tortato FR, Santos FM, Chaves FG, Tirelli FP, Vilas Boas FE, Rodrigues FHG, Ubaid FK, Grotta-Neto F, Palomares F, Souza FL, Costa FE, França FGR, Ramírez Pinto F, Aguiar GL, Hofmann GS, Heliodoro G, Duarte GT, Ribeiro de Andrade G, Beca G, Zapata-Ríos G, Giné GAF, Powell GVN, Wilson Fernandes G, Forero-Medina G, Melo GL, Santana GG, Ciocheti G, Alves GB, Souto GHBO, Villarroel GJ, Porfirio GEO, Batista GO, Behling GM, Ayala Crespo GM, Mourão GM, Rezende GZ, Toledo GADC, Herrera HM, Alves Prado H, Bergallo HG, Secco H, Rajão H, Roig HL, Concone HVB, Duarte H, Ermenegildo H, Ferreira Paulino Neto H, Quigley H, Lemos HM, Cabral H, Fernandes-Ferreira H, Del Castillo HF, Ribeiro IK, Coelho IP, Franceschi IC, Melo I, Oliveira-Bevan I, Mourthe I, Bernardi I, de la Torre JA, Marinho-Filho J, Martinez J, Palacios Perez JX, Pérez-Torres J, Bubadué J, Silveira JR, Seibert JB, Oliveira JF, Assis JR, De la Maza J, Hinojosa J, Metzger JP, Thompson JJ, Svenning JC, Gouvea JA, Souza JRD, Pincheira-Ulbrich J, Nodari JZ, Miranda J, Zecchini Gebin JC, Giovanelli JGR, Rossi Junior JL, Pandini Favoretti JP, Villani JP, Just JPG, Souza-Alves JP, Costa JF, Rocha J, Polisar J, Sponchiado J, Cherem JJ, Marinho JR, Ziegler J, Cordeiro J, de Sousa E Silva Júnior J, Rodriguez-Pulido JA, Chaves Dos Santos JC, Dos Reis Júnior JC, Mantovani JE, Moreira Ramírez JF, Sarasola JH, Cartes JL, Duarte JMB, Longo JM, Dantas JO, Venancio JO, de Matos JR, Pires JSR, Hawes JE, Santos JG, Ruiz-Esparza J, Martínez Lanfranco JA, Rudolf JC, Charre-Medellin JF, Zanón-Martínez JI, Peña-Mondragón JL, Campos Krauer JM, Arrabal JP, Beduschi J, Ilha J, Mata JC, Bonanomi J, Jordao J, de Almeida-Rocha JM, Pereira-Ribeiro J, Zanoni JB, Bogoni JA, Chacón Pacheco JJ, Contreras Palma KM, Strier KB, Rodriguez Castro KG, Didier K, Schuchmann KL, Chávez-Congrains K, Burs K, Ferraz KMPMB, Juarez KM, Flesher K, Morais KDR, Lautenschlager L, Grossel LA, Dahmer LC, de Almeida LR, Fornitano L, Barbosa LNB, Bailey LL, Barreto LN, Villalba LM, Magalhães LM, Cullen L Jr, Marques L, Marques Costa L, Silveira L, Moreira LS, Sartorello L, Oliveira LC, Gomes LP, Aguiar LDS, da Silva LH, Mendonça LS, Valenzuela LA, Benavalli L, Dias LCS, Munhoes LP, Catenacci L, Rampim LE, de Paula LM, Nascimento LA, Gonçalves da Silva L, Quintilham L, Ramis Segura L, Perillo LN, Rezende LR, Martínez Retta L, Rojas LNS, Guimarães LN, Araújo L, Zago da Silva L, Querido LCA, Verdade LM, Perera-Romero LE, Carvalho-Leite LJ, Hufnagel L, Rezende Bernardo LR, Oliveira LF, Oliveira Santos LGR, Lyra LH, Borges LHM, Severo MM, Benchimol M, Quatrocchi MG, Martins MZA, Rodrigues M, Penteado MJF, Figuerêdo Duarte Moraes M, Oliveira MA, Lima MGM, Pônzio MDC, Cervini M, da Silva M, Passamani M, Villegas MA, Dos Santos Junior MA, Yamane MH, Jardim MMA, Leite de Oliveira M, Silveira M, Tortato MA, Figueiredo MSL, Vieira MV, Sekiama ML, Andrade da Silva MA, Nuñez MB, Siviero MB, Carrizo MC, Barros MC, Barros MAS, do Rosário MCF, Peñuela Mora MC, Fleytas Jover MDC, Morandi MEF, Huerta ME, Fernandes MEA, Viscarra Siñani ME, Iezzi ME, Ramos Pereira MJ, Gomez Vinassa ML, Lorini ML, Jorge MLSP, Morini MS, Guenther M, Landis MB, Vale MM, Xavier MS, Tavares MS, Kaizer M, Velilla M, Bergel MM, Hartmann MT, Lima da Silva M, Rivero M, Salles Munerato M, Xavier da Silva M, Zanin M, Marques MI, Haberfeld M, Di Bitetti MS, Bowler M, Galliez M, Ortiz-Moreno ML, Buschiazzo M, Montes MA, Alvarez MR, Melo-Dias M, Reis MG, Corrêa MRJ, Tobler MW, Gompper ME, Nunez-Regueiro M, Brandão Vecchi M, Graipel ME, Godoi MN, Moura MO, Konzen MQ, Pardo MV, Beltrão MG, Mongelli M, Almeida MO, Gilmore MP, Schutte M, Faria MB, Luiz MR, de Paula M, Hidalgo-Mihart MG, Perilli MLL, Freitas-Junior MC, da Silva MP, Denkiewicz NM, Torres NM, Olifiers N, De Lima NDS, de Albuquerque NM, Canassa NF, de Almeida Curi NH, Prestes NP, Falconi N, Gurgel-Filho NM, Pasqualotto N, Cáceres NC, Peroni N, de la Sancha NU, Zanella N, Monroy-Vilchis O, Pays O, Arimoro OA, Ribeiro OS, Villalva P, Gonçalves PR, Santos PM, Brennand P, Rocha P, Akkawi P, Cruz P, Ferreira PM, Prist PR, Martin PS, Arroyo-Gerala P, Auricchio P, Hartmann PA, Antas PTZ, Camargo PHSA, Marinho PH, Ruffino PHP, Prado PI, Martins PW, Cordeiro-Estrela P, Luna P, Sarmento P, Faria Peres PH, Galetti PM Jr, de Castilho PV, Renaud PC, Scarascia PO, Cobra PPA, Lombardi PM, Bessa R, Reyna-Hurtado R, de Souza RCC, Hoogesteijn RJ, Alves RSC, Romagna RS, Silva RL, de Oliveira R, Beltrão-Mendes R, Alencar RM, Coutinho R, da Silva RC, Caribé Grando RLSC, Matos RG, Araujo RDS, Pedroso RF, Durães RMN, Ribeiro RLA, Chagas R, Miotto R, Twardowsky Ramalho Bonikowski R, Muylaert RL, Pagotto RV, Hilário RR, Faria RT, Bassini-Silva R, Sampaio R, Sartorello R, Pires RA, Hatakeyama R, Bianchi RC, Buitenwerf R, Wallace R, Paolino RM, Fusco-Costa R, Trovati RG, Tomasi RJ, Espíndola Hack RO, Magalhães RA, Nobrega RAA, Nobre RA, Massara RL, Fróes RM, Araújo RPDC, León Pérez RR, Jorge RSP, de Paula RC, Martins R, da Cunha RGT, Costa R, Alves RRN, Garcia-Anleu R, Santos Almeida RP, Cueva Loachamín RD, Andrade RS, Juárez R, Bordallo SU, Guaragni SA, Carrillo-Percastegui SE, Seber S, Astete S, Hartz SM, Espinosa S, Álvarez Solas S, Ramos Lima S, Silvestre SM, Machado SAS, Keuroghlian-Eaton S, Albanesi S, Costa SA, Bazilio S, Mendes SL, Althoff SL, Pinheiro SD, Napiwoski SJ, Fernández Ramirez S, Talamoni SA, Age SG, Pereira TC, Moreira TC, Trigo TC, Gondim TMDS, Karlovic TC, Cavalcante T, Maccarini T, Rodrigues TF, de Camargo E Timo TP, Monterrubio TC, Piovezan U, Cavarzere V, Towns V, Onofrio VC, Oliveira VB, Araújo VC, Melo VL, Kanaan VT, Iwakami V, Vale V, Picinatto Filho V, Alberici V, Bastazini VAG, Orsini VS, Braz VDS, Rojas Bonzi VB, Guedes Layme VM, Gaboardi VTR, Rocha VJ, Martins WP, Tomas WM, Hannibal W, Dáttilo W, Silva WR, Endo W, Bercê W, Bravata de la Cruz Y, Ribeiro YGG, Galetti M, and Ribeiro MC
- Subjects
- Animals, Ecosystem, Humans, Canidae, Carnivora, Mustelidae, Ursidae
- Abstract
Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non-detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non-governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peer-reviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non-detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio-temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other large-scale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data., (© 2020 The Authors. Ecology © 2020 The Ecological Society of America.)
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- 2020
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33. NEOTROPICAL ALIEN MAMMALS: a data set of occurrence and abundance of alien mammals in the Neotropics.
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Rosa CAD, Ribeiro BR, Bejarano V, Puertas FH, Bocchiglieri A, Barbosa ALDS, Chiarello AG, Paglia AP, Pereira AA, Moreira AFS, Souza AC, Pellegrin A, Gatica A, Medeiro AZ, Pereira AD, Braz AG, Yanosky A, Valenzuela AEJ, Bertassoni A, Prado ADSVD, Nava AFD, Rocha A, Bovo AAA, Bager A, Cravino A, Pires ADS, Martensen AC, Filippini A, Percequillo AR, Vogliotti A, Antunes AZ, Leite de Oliveira AC, da Silva de Oliveira AJ, Devlin A, de Paula A, Ferreira AS, García-Olaechea A, Subalusky A, Sánchez A, de Aquino ACMM, Srbek-Araujo AC, Paldês Gonçales A, Araújo ACL, Gozzi AC, Ochoa AC, Mendes de Oliveira AC, Lacerda ACR, Francisco AK, Paschoal AMO, Gomes APN, Potrich AP, Olímpio APM, Rojas A, Meiga AYY, Jácomo ATA, Calaça AM, Feijó A, Pagoto A, Borja Miranda A, Chein Alonso A, Barreto-Lima AF, Lanna A, Luza AL, Camilo AR, Tavares A, Nunes AV, Kindel A, de Miguel A, Gatti A, Nobre AB, Campêlo ADC, Albuquerque ACF, de la Torre A, Mangione A, Mendes Pontes AR, Fernandes AS, Felicio ALA, Ferreguetti AC, Marcili A, Piratelli AJ, Nascimento AGSD, Banhos Dos Santos Á, Rosa BF, Cezila BA, de Thoisy B, Ingberman B, Köhler B, Morais BC, Gómez-Valencia B, Bertagni de Camargo B, Bezerra BM, Tamasauskas B, Parahyba Campos BAT, Kubiak BB, Saranholi BH, Nakagawa BK, Leles BP, Lim BK, Pereira Mendes C, Islas CA, Aoki C, Cantagallo Devids C, Figueiredo C, Abreu CMG, Silva Oliveira CR, Cassano CR, Lugarini C, Caputo C, Gestich CC, Tedesco CD, Vera Y Conde CF, Hegel CGZ, Kasper CB, De Angelo C, Grelle CEV, Fragoso CE, Esbérard CEL, Rocha CFD, Verona CE, Salvador CH, Vieira CL, Abrahão CR, Brocardo CR, Fieker CZ, Braga C, Sánchez Lalinde C, Bueno C, Ikuta CY, Luna CLB, Cestari C, Del Vechio Koike C, Knogge C, Anderson CB, Hurtado CM, Ferreira Antunes de Oliveira C, Tellaeche C, Cesário CS, Costa CG, Kanda CZ, Costa SA, Seixas CS, Trinca CT, López-Fuerte CF, da Cunha CJ, Doutel Ribas C, Santos CC, Buscariol D, Carreira D, Nascimento DCD, Carvalho DR, Ferraz DDS, Galiano D, Homem DH, Jesús-Espinosa D, Bôlla DAS, Moreno DJ, Moreira DO, Ramos DL, de Amorim DA, Barros-Battesti DM, Lopez DE, Tavares DC, Post DM, Couto DR, Patrocínio DN, Carvalho DLKP, Silva DA, Córdoba D, Queirolo D, Varela D, de Oliveira DAG, Casanova DC, Dias DM, Machado da Silva D, Barbier E, Rivadeneira EF, Alexandrino E, Carrano E, Santos EM, Venticinque EM, Hernández-Pérez E, Casazza EDF, Anderson EP, Fraga EDC, de Lima EF, D'Bastiani E, Vieira EM, Guijosa-Guadarrama E, González EM, Maggiorini EV, Aguiar EFS, Martínez-Nambo ED, Castro ÉP, de la Peña-Cuéllar E, Pedó E, Melo FCSA, Rocha FL, Fonseca FL, Girardi F, Melo FR, Roque FO, Keesen Ferreira F, Peters FB, Moreli Fantacini F, Pedrosa F, Pessoa da Silva F, Vélez-García F, Abra FD, de Azevedo FC, Guedes da Silva F, Neri FM, Teixeira FZ, Fernandez FADS, Carvalho F, Passos FC, Jacinavicius FC, Ferreira F, Pinho FF, Gonçalves F, Ibanez Martins F, Lima F, Contreras-Moreno FM, Ribeiro FS, Tortato F, Patel FM, Caruso F, Tirelli FP, Rodrigues FHG, Ubaid FK, Palmeira FBL, Grotta Neto F, Gabriel FH, de Souza FL, Costa FEDVD, de Aguiar GL, Lemos FG, Magezi GS, Panigai GFVD, Hofmann GS, Heliodoro G, Rosa Graviola G, Beca G, Andrade GR, Jiménez Romero G, Duarte GT, Melo GL, Dierings GL, Sabino-Santos G Jr, de Oliveira GL, Santana GG, Ciocheti G, Zanirato GL, Alves GB, Batista GO, Behling GM, Ferreira GB, da Rocha GC, Lessa G, Mourão G, Maras GA, Toledo GADC, Gonsioroski G, Canale GR, Schuchmann KL, Sebastião H, Alves do Prado H, Bergallo HG, Secco HKC, Roig HL, Rajão H, Carlos HSA, Duarte HOB, Ermenegildo H, Pena HFJ, Entringer Júnior H, Paulino Neto HF, Lemos HM, Del Castillo H, Fernandes-Ferreira H, Coitiño Banquero HI, Roesler I, Ribeiro IK, Coelho IP, Lima IMS, Bechara IM, Lermen IS, Mella Méndez I, Schuck G, Esperandio IB, Silva IO, Mourthe I, Oliveira I, Bernardi IP, Miller JR, Marinho-Filho J, Zocche JJ, Russell JC, Seibert JB, Hinojosa J, Vitule JRS, Thompson JJ, Silva JCR, Gouvea JA, Santos JP, Falcão JCF, Castro-Prieto J, Ferreira JP, Pincheira-Ulbrich J, Nodari JZ, Zecchini Gebin JC, Giovanelli JGR, Miranda JMD, Souza-Alves JP, Marins JRGA, Costa JF, Sponchiado J, de Souza JL, Gallo JA, Cherem JJ, Cordeiro JLP, Duarte JMB, Dantas JO, de Matos JR, Pires JSR, Martínez Lanfranco JA, de la Cruz Godoy JC, Rudolf JC, Parrish JFR, Tellarini JF, Peña-Mondragón JL, Arrabal JP, Reppucci J, Ruiz-Esparza J, Beduschi J, Oshima JEF, Ribeiro JF, Almeida Rocha JM, Ferreira Neto JS, Silveira Dos Santos J, Pereira-Ribeiro J, Zanoni JB, Bogoni JA, Ferreira JR, Bicca-Marques JC, Chacón Pacheco JJ, Scarascia PO, Guidoni-Martins KG, Burs K, Ferraz KMPMB, Pisciotta KR, Silva KVKA, Juarez KM, de la Cruz-Félix K, de Morais KDR, Candelária LP, Fornitano L, Bailey LL, Gonçalves LO, Fasola L, Nova León LJ, de Andrade LR, Marques LO, Macedo L, Moreira LS, Silveira L, Oliveira LC, da Silva LH, Jerusalinsky L, La Serra L, Marques Costa L, Sartorello LR, Munhoes LP, Oliveira-Silva LRB, de Pina LF, Bonjorne L, Rampim LE, Sales LP, Gonçalves da Silva L, Quintilham LLT, Perillo LN, Rodríguez-Planes LI, Martín L, Araújo LS, Tiepolo LM, Zago Silva L, García Loaiza LM, Querido LCA, da Silva LF, La Sala LF, Bopp LT, Hufnagel L, Oliveira LFB, Oliveira-Santos LGR, Lyra LH, Guimarães LN, Jimenez Segura LF, de Sousa LC, Möcklinghoff L, Guichón ML, de la Maza J, Barrios-Garcia MN, Talamoni SA, Severo MM, Martins MZA, Oliveira MA, Figuerêdo Duarte Moraes M, Lima MGM, Soares Pinheiro M, Pônzio MDC, Guerreiro M, Cervini M, da Silva M, Oliveira MJR, Magioli M, Passamani M, Silva de Almeida M, Amaku M, Leite de Oliveira M, Tortato MA, Melo MA, Coutinho ME, Dantas Santos MP, Vieira MV, Andrade MA, Barros MC, Rosario MCFD, Domit MDADS, Fernandes MEA, Iezzi ME, do Nascimento MHS, Andrade-Núñez MJ, Lorini ML, Morini MSC, Nagy-Reis MB, Landis MB, Vale MM, Xavier MS, Kaizer MC, Baptiste MP, Bergel MM, Borgnia M, Barros MAS, Lima da Silva M, Favarini MO, Sales Munerato M, Zaluar MT, Winter M, Xavier da Silva M, Zanin M, Marques MI, Haberfeld MB, Di Bitetti MS, Galliez M, Alvarez MR, Malerba M, Rivero M, Melo Dias M, de Oliveira MY, Dos Reis MG, Corrêa MRJ, Graipel ME, Godoi MN, Núñez-Regueiro MM, Moura MO, Orsi ML, Galvão da Silva MA, Sanvicente Lopez M, Benedetti MA, Beltrão MG, Camino M, Faria MB, Miretzki M, Luiz MR, Perine M, Monteiro MCM, Alves-Eigenheer M, Perilli MLL, da Silva MA, Marini MÂ, Silva Pereira M, de Freitas Junior MC, Cossa N, Denkiewicz NM, Tôrres NM, Olifiers N, de Albuquerque NM, Canassa NF, Detogne N, Gurgel Filho N, Seoane NF, da Rosa Oliveira N, Megale N, Pasqualotto N, Cáceres NC, Peroni N, Zanella N, Pays O, Arimoro OAS, Acevedo-Charry O, de Almeida Curi NH, Pinha PRS, Perovic P, Gonçalves PR, Santos PM, Brennand PGG, Kerches Rogeri P, Rosas Ribeiro P, da Rocha PA, de Lázari PR, Pedreira PA, Pinheiro PF, Lira PK, Ferreira PM, Martin PS, Antas PTZ, Marinho PH, Ruffino PHP, Camargo PHSA, Landgref Filho P, Mangini PR, Farias P, Cordeiro-Estrela P, de Faria Peres PH, Galetti PM Jr, Ramírez-Bautista P, Maués PCRA, Renaud PC, Sartorello R, Barros PA, Lombardi PM, Bessa R, Arroyo-Gerala P, de Souza RCC, Zenni RD, Flores Peredo R, Hoogesteijn R, Loyola R, Alves RSC, Rodarte RRP, Silva RL, de Oliveira R, Beltrão-Mendes R, Alencar RM, da Silva RC, Pedroso R, Sampaio RF, Ribeiro RLA, Pardini R, Twardowsky Ramalho Bonikowski R, Pagotto RV, Dias RA, Bassini-Silva R, Corassa Arrais R, Sampaio R, de Cassia Bianchi R, Paolino RM, Fusco-Costa R, Trovati RG, Espíndola Hack RO, Mauro RA, Nobre RA, Gessulli RD, León Pérez R, Massara RL, Fróes da Silva RM, de Paula RC, da Cunha RGT, Costa RT, Marques RV, Morato RG, Bovendorp RS, Dornas RADP, Andrade RS, Siciliano S, Guaragni SA, Rolim SG, Astete S, Cavalcanti S, Hartz SM, Carvalho S, Cortez S, Silvestre de Sousa SM, Ballari SA, Ramos Lima S, Cirignoli S, García-R S, Bazilio S, Solari Torres S, Back Franco S, Martins SR, de Bustos S, Age SG, Ferrari SF, Francisco TM, Micheletti T, Godim TMDS, Luiz TG, Ochotorena de Freitas TR, Rodrigues TF, Piovezan U, Barcos UC, Onofrio VC, Martin-Albarracin VL, Towns V, Araújo VC, Kanaan V, Daga VS, Boere V, de Araujo VPG, Benitez VV, Leandro-Silva V, Geraldi VC, Alberici V, Bastazini VAG, Gasparotto VPO, Orsini VS, da Silva VS, Rojas Bonzi V, Pereira VJA, Layme VMG, Duarte da Silva VH, Tomas WM, Moreira TA, Martins WP, de Moraes Pires WM, Hannibal W, Dáttilo W, Mottin V, Endo W, Bercê W, Carvalho WD, Magnusson W, Akkawi P, Di Blanco Y, Amaral PR, Ramos YGC, Rodríguez-Calderón YG, Mendes YR, Ribeiro YGG, Campos Z, Galetti M, and Ribeiro MC
- Subjects
- Animals, Argentina, Biodiversity, Cattle, Chile, Dogs, Florida, Mexico, Introduced Species, Mammals
- Abstract
Biological invasion is one of the main threats to native biodiversity. For a species to become invasive, it must be voluntarily or involuntarily introduced by humans into a nonnative habitat. Mammals were among first taxa to be introduced worldwide for game, meat, and labor, yet the number of species introduced in the Neotropics remains unknown. In this data set, we make available occurrence and abundance data on mammal species that (1) transposed a geographical barrier and (2) were voluntarily or involuntarily introduced by humans into the Neotropics. Our data set is composed of 73,738 historical and current georeferenced records on alien mammal species of which around 96% correspond to occurrence data on 77 species belonging to eight orders and 26 families. Data cover 26 continental countries in the Neotropics, ranging from Mexico and its frontier regions (southern Florida and coastal-central Florida in the southeast United States) to Argentina, Paraguay, Chile, and Uruguay, and the 13 countries of Caribbean islands. Our data set also includes neotropical species (e.g., Callithrix sp., Myocastor coypus, Nasua nasua) considered alien in particular areas of Neotropics. The most numerous species in terms of records are from Bos sp. (n = 37,782), Sus scrofa (n = 6,730), and Canis familiaris (n = 10,084); 17 species were represented by only one record (e.g., Syncerus caffer, Cervus timorensis, Cervus unicolor, Canis latrans). Primates have the highest number of species in the data set (n = 20 species), partly because of uncertainties regarding taxonomic identification of the genera Callithrix, which includes the species Callithrix aurita, Callithrix flaviceps, Callithrix geoffroyi, Callithrix jacchus, Callithrix kuhlii, Callithrix penicillata, and their hybrids. This unique data set will be a valuable source of information on invasion risk assessments, biodiversity redistribution and conservation-related research. There are no copyright restrictions. Please cite this data paper when using the data in publications. We also request that researchers and teachers inform us on how they are using the data., (© 2020 The Authors. Ecology © 2020 The Ecological Society of America.)
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- 2020
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34. Comparison Between Three Cryoprotectants in the Freezing of Mazama americana Semen Collected by Artificial Vagina.
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Alvarez MCL, Rola LD, and Duarte JMB
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- Animals, Cryopreservation, Cryoprotective Agents, Female, Freezing, Glycerol, Humans, Male, Sperm Motility, Spermatozoa, Vagina, Semen, Semen Preservation
- Abstract
Maintaining genetic variability is an important part of the conservation of endangered species, so the construction of germplasm banks is essential. Several species of the genus Mazama endure constant pressure in their natural habitat and are threatened with extinction. The correct manipulation and adequacy of the diluents and cryoprotectants must be studied to be successful in the formation of these banks. The purpose of this study was to evaluate the efficiency of three different cryoprotectants in sperm cryopreservation in the species Mazama americana : 6% glycerol (GLY), 3% ethylene glycol (ETG), and 5% dimethylformamide (DMF). Semen was obtained with the lateral deviation of the penis to an artificial vagina. In the pre-freeze and post-thaw periods, motility, vigor, membrane integrity, acrosome integrity, and sperm cell morphology were evaluated for each of the cryoprotectants. Post-thaw motility was higher when semen was frozen with cryoprotectants GLY and DMF (55.31 ± 7.39 and 55.94 ± 2.77, respectively), compared with the result obtained for ETG (48.13 ± 2.39). For major defects (MaD), a difference was observed between the pre- and post-cryopreservation periods, such that DMF generated a higher number of post-thaw MaD (25.94 ± 5.37). All cryoprotectants were efficient for cryopreservation of M. americana semen, resulting in samples with satisfactory viability after thawing. However, the medium with the cryoprotectants GLY, at a concentration of 6%, and DMF, at a concentration of 5%, were preferable.
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- 2020
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35. Designation of a neotype for Mazama americana (Artiodactyla, Cervidae) reveals a cryptic new complex of brocket deer species.
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Cifuentes-Rincón A, Morales-Donoso JA, Sandoval EDP, Tomazella IM, Mantellatto AMB, de Thoisy B, and Duarte JMB
- Abstract
Mazama americana (red brocket deer) is the genus-type species (first species described for this genus) and the basis for the identity of other Mazama species. Mazama americana is one of the most abundant and widely distributed deer species in the neotropical forest. However, recent studies suggest that this taxon belongs to a species complex. Our goal was to collect an animal at the type locality (topotype) in French Guiana with the aim of characterizing the morphological (biometric, craniometric), cytogenetic (Giemsa, C-banding, G-banding and NOR) and molecular (mitochondrial DNA) features. The comparisons showed that the collected specimen was very similar morphologically to specimens from other South American populations, but it was cytogenetically and molecularly very different from any of the cytotypes already described for this species, corroborating the existence of a complex of cryptic species. The data suggest that the M. americana topotype is a different species from all the cytotypes already described in the literature and which occupy the southern region of the Amazon River. The characterization and designation of the M. americana neotype is the first step toward a taxonomic reorganization of the genus Mazama , with the potential identification of new species., (Analorena Cifuentes-Rincón, Jorge Alfonso Morales-Donoso, Eluzai Dinai Pinto Sandoval, Iara Maluf Tomazella, Aline Meira Bonfim Mantellatto, Benoit de Thoisy, José Maurício Barbanti Duarte.)
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- 2020
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36. In Vitro Development and Mitochondrial Gene Expression in Brown Brocket Deer ( Mazama gouazoubira ) Embryos Obtained by Interspecific Somatic Cell Nuclear Transfer.
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Magalhães LC, Cortez JV, Bhat MH, Sampaio ACNPC, Freitas JLS, Duarte JMB, Melo LM, and Freitas VJF
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- Animals, Blastocyst metabolism, Cattle, Cells, Cultured, Cloning, Organism veterinary, Electron Transport Complex IV genetics, Electron Transport Complex IV metabolism, Embryo, Mammalian metabolism, Female, Fertilization in Vitro, Goats, Mitochondrial Proton-Translocating ATPases genetics, Mitochondrial Proton-Translocating ATPases metabolism, Morula metabolism, NADH Dehydrogenase genetics, NADH Dehydrogenase metabolism, Nuclear Transfer Techniques veterinary, Oocytes metabolism, Up-Regulation, Deer embryology, Deer genetics, Embryonic Development, Gene Expression Regulation, Developmental, Genes, Mitochondrial
- Abstract
The genetic diversity of Neotropical deer is increasingly jeopardized, owing to declining population size. Thus, the formation of cryobanking of somatic cells is important for the preservation of these species using cloning. The transformation of these cells into viable embryos has been hampered by a lack of endangered species oocytes. Accordingly, the aim of this study was to produce brown brocket deer embryos by interspecific somatic cell nuclear transfer (iSCNT), using goat or cattle oocytes as cytoplasts, and to elucidate embryo mitochondrial activity by measuring the expression levels of ATP6 , COX3 , and ND5 . Cattle embryos produced by in vitro fertilization (IVF) were used as a control. There were no differences in the development of embryos produced by traditional SCNT and iSCNT when using either the goat cytoplasts (38.4% vs. 25.0% cleaved and 40.0% vs. 50.0% morula rates, respectively) or cattle cytoplast (72.8% vs. 65.5% cleaved and 11.3% vs. 5.9% blastocyst rates, respectively). Concerning the gene expression, no significant difference was observed when goat oocytes were used as cytoplasts. However, when using cattle oocytes and 16S as a reference gene, the iSCNT upregulated COX3 , when compared with SCNT group. In contrast, when GAPDH was used as a reference gene, all the evaluated genes were upregulated in the iSCNT group, when compared with the IVF group. When compared with the SCNT group, only the expression of ATP6 was statistically different. In conclusion, it was demonstrated that interspecific nuclear transfer is a potentially useful tool for conservation programs of endangered similar deer species.
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- 2020
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37. Protein profile of the ovarian follicular fluid of brown brocket deer ( Mazama gouazoubira ; Fisher, 1814).
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Souza TTS, Bezerra MJB, van Tilburg MF, Nagano CS, Rola LD, Duarte JMB, Melo LM, Moura AA, and Freitas VJF
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- Animals, Female, Follicular Fluid, Ovarian Follicle, Ovulation Induction, Deer
- Abstract
The aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (≤3.5 mm) and large (>3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P > 0.05) was observed between small/medium and large follicles: 49.2 ± 22.8 and 56.7 ± 27.4 μg/μl, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P > 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer.
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- 2020
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38. Molecular identification of Mazama species (Cervidae: Artiodactyla) from natural history collections.
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Mantellatto AMB, González S, and Duarte JMB
- Abstract
Natural history museum collections constitute an invaluable patrimony of biological diversity for analysing the taxa distribution and evolution. However, it is very common to discover taxonomic misidentification in museum collections based on incorrect data. The aim of this research was to identify brocket deer species (Mazama genus) using molecular markers. We collected 199 samples, performed DNA extraction and species identification using a specific mitochondrial marker based on a fragment of cytochrome b (Cytb) for Neotropical deer. We achieved the amplification and sequencing of 77 specimens and verified that 26% of the skulls were wrongly identified. Moreover, in the museum collections 57% of the specimens were only identified as Mazama sp, and we were able to identify them by molecular methods to the species level. Our findings clearly demonstrate the importance of integrating molecular analyses to identify Mazama species, since using only external morphology can result in a high probability of errors. We recommend the selection of non-convergent morphological characters, which together with the use of DNA collected from museum specimens should contribute to more accurate taxonomic identifications.
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- 2020
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39. New records and genetic diversity of Mycoplasma ovis in free-ranging deer in Brazil.
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André MR, Duarte JMB, Gonçalves LR, Sacchi ABV, Jusi MMG, and Machado RZ
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- Animals, Brazil, Cluster Analysis, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Genotype, Mycoplasma genetics, Mycoplasma Infections epidemiology, Mycoplasma Infections microbiology, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Animal Diseases epidemiology, Animal Diseases microbiology, Deer microbiology, Genetic Variation, Mycoplasma classification, Mycoplasma isolation & purification, Mycoplasma Infections veterinary
- Abstract
Cervids represent a mammal group which plays an important role in the maintenance of ecological balance. Recent studies have highlighted the role of these species as reservoirs for several arthropods-borne pathogens. Globally, hemotropic mycoplasmas (haemoplasmas) are emerging or remerging bacteria that attach to red blood cells of several mammals species causing hemolytic anaemia. Therefore, the aim of this study was to investigate the occurrence and assess the phylogenetic positioning of Mycoplasma ovis in free-ranging deer from Brazil. Using a polymerase chain reaction targeting the 16S rRNA region, 18 (40%) out of 45 sampled deer were positive to M. ovis. Among the nine sequences analysed, four distinct genotypes were identified. The sequences detected in the present study were closely related to sequences previously identified in deer from Brazil and the USA. On the other hand, the Neighbour-Net network analysis showed that the human-associated M. ovis genotypes were related to genotypes detected in sheep and goats. The present study shows, for the first time, the occurrence of M. ovis in Mazama gouazoubira and Mazama bororo deer species, expanding the knowledge on the hosts harbouring this haemoplasma species. Once several deer species have your population in decline, additional studies are needed to evaluate the pathogenicity of M. ovis among deer populations around the world and assess its potential as reservoir hosts to human infections.
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- 2020
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40. Stress in captive Blue-fronted parrots ( Amazona aestiva ): the animalists' tale.
- Author
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Vidal AC, Roldan M, Christofoletti MD, Tanaka Y, Galindo DJ, and Duarte JMB
- Abstract
Understanding stress physiology is crucial for species management because high levels of stress can reduce reproduction and the individual's ability to face threats to survive. One of the most popular methods for non-invasive monitoring of animal endocrine status is the glucocorticoid (GC) metabolite measurements, which can provide important information about how animals are affected by their surrounding environment. Here, we carried out the biological validation of corticosterone enzyme immunoassays (EIAs), which together with a cortisol EIA was used to quantified the concentrations of urofaecal GC metabolites (uGCMs) in wild and captive Blue-fronted amazon parrots ( Amazona aestiva ). Urofaecal GC concentrations were significantly higher ( P < 0.05) in free-living parrots (157.9 ± 18.5 ng cortisol/g and 61.14 ± 23.5 ng corticosterone/g dry urofaecal sample) than in those kept in captivity, which showed the comparable levels of GC metabolites independently of the management system applied. The higher uGCM levels obtained in the wild population point to an adaptive response for survival and species propagation in a more challenging environment, in comparison with captive animals. Furthermore, the lower uGCM concentrations in captive parrots may indicate an adaptive capacity of the species A. aestiva to captivity and its potential as a legal pet. The corticosterone EIA applied in this study proved to be an effective technique for the adrenocortical activity monitoring in this species. We discuss our findings considering the management and destiny given to wild-caught birds that are kept in confinement or returned to nature., (© The Author(s) 2019. Published by Oxford University Press and the Society for Experimental Biology.)
- Published
- 2019
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41. Non-homogeneous distribution of steroids in fecal pellets: An example in brown brocket deer (Mazama gouazoubira) with progesterone metabolites.
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Tanaka Y, Sandoval EDP, and Duarte JMB
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- Animals, Drug Implants, Female, Hormones metabolism, Reproduction, Deer metabolism, Feces chemistry, Metabolome, Progesterone metabolism
- Abstract
Measuring reproductive hormones in feces has become an important tool in the endocrine characterization of wild animals' reproduction. However, several factors may influence its success, such as fecal collection and storage techniques, knowledge of steroid hormone metabolism, the extraction procedure, immunoassay selection, inherent factors, and the distribution of steroid hormones in the feces. It is known that the distribution of these hormones in the feces is not homogeneous, and prior to the extraction of the steroidal metabolites, homogenization of the feces is recommended. Hormonal analysis is based on only a small fraction of the feces, which in theory should be representative of the total. In the case of cervids and other ruminants, feces consist of pellets. Here, the concentration of the steroid metabolites of each pellet was measured in order to evaluate the distribution of the fecal progesterone metabolites concentration in 10 pellets/fecal mass from five female Mazama gouazoubira. There were large variations in fecal progesterone metabolites concentrations between the pellets of the same feces/female, showing the following amplitude variations: Animal A: 112%; Animal B: 164%; Animal C: 115%; Animal D: 62%; Animal E: 108%. These results show the importance of adequate homogenization prior to steroid metabolite extraction., (Copyright © 2019 Elsevier Inc. All rights reserved.)
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- 2019
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42. Evidence of exposure to Coxiella burnetii in neotropical free-living cervids in South America.
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Zanatto DCS, Duarte JMB, Labruna MB, Tasso JB, Calchi AC, Machado RZ, and André MR
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- Animals, Brazil epidemiology, Coxiella burnetii genetics, Floods, Fluorescent Antibody Technique, Indirect veterinary, Humans, Immunoglobulin G blood, Polymerase Chain Reaction veterinary, Q Fever epidemiology, Rivers, Ticks, Antibodies, Bacterial blood, Coxiella burnetii immunology, DNA, Bacterial blood, Deer, Q Fever veterinary
- Abstract
Coxiella burnetii (order Legionellales, family Coxiellaceae), the etiological agent of Q fever, is a pleomorphic, obligate Gram-negative intracellular bacillococcus that can infect humans and animals. Among the mammals hosting this agent, both domestic and wild ruminants are of particular economic and public health importance. Ticks and other hematophagous arthropods or aerosols are incriminated in the transmission between reservoirs and susceptible hosts. This study used serological and molecular methods to investigate the C. burnetii occurrence in blood samples from free-living deer (143 Blastocerus dichotomus, 27 Mazama gouazoubira, 4 M. bororo, 3 M. americana, and 11 Ozotocerus bezoarticus) sampled in Mato-Grosso do Sul, São Paulo, Goiás and Paraná states in Brazil. The DNA extracted from the blood samples of 188 cervids was submitted to nested (n) PCR for C. burnetii based on the IS1111 repetitive insertion element of the heat shock protein (htpAB) gene. Additionally, 169 serum samples were submitted to Indirect Immunofluorescence Assay (IFAT) to detect Ig antibodies to C. burnetii. The nPCR results indicated that all blood samples were negative, evidencing the absence of circulating C. burnetii DNA in cervids from the studied regions or, alternatively, the C. burnetii DNA concentration in the deer blood samples was below the threshold of the used PCR technique. On the other hand, 5.32% (9/169) of deer were seropositive for C. burnetii by IFAT, with titers ranging from 256 and 16,384. In conclusion, the present work showed the first evidence of exposure of free-living deer to C. burnetii in Brazil., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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43. Azaperone and xylazine: A pharmacological combination to facilitate captive deer management for red brocket deer (Mazama americana).
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Carregaro AB, Ferrari BG, da Silva ANE, Xavier NV, and Duarte JMB
- Subjects
- Animals, Dose-Response Relationship, Drug, Female, Heart Rate drug effects, Male, Respiration drug effects, Azaperone pharmacology, Deer, Immobilization methods, Xylazine pharmacology
- Abstract
The care and management of deer in captivity is challenging, especially in the case of red brocket deer (Mazama americana), whose routine management using physical restraint is difficult. Our study evaluated the effects of azaperone and xylazine combination for immobilizing red brocket deer and allow for the standard capture and handling protocols (e.g., biological material, horn cutting, and trimming) to be conducted safely. Six adult, captive, red brocket deer received an intramuscular injection of either 1 mg/kg azaperone and 0.5 mg/kg xylazine (AX0.5) or 1 mg/kg azaperone and 1 mg/kg xylazine (AX1.0). Sedation latency, sternal recumbency, safe handling, and quality of the sedation were evaluated to provide an overview of how the immobilizing drugs affected managing the species in captivity. Additionally, heart rate, respiratory rate, mean arterial pressure, rectal temperature, pH, PaO2, PaCO2, SaO2, HCO3-, BE, Na+, K+ and serum lactate were also measured. The latency period of the animals in the AX0.5 group was greater than that of the animals in the AX1.0 group (7 ± 6.6 min vs. 5 ± 2.0 min), as was the time for them to assume sternal recumbency (12 ± 9.7 min vs. 6 ± 3.1 min). However, the time after the initial dose at which the animals could safely be handled (14 ± 4.5 min vs. 12 ± 5.2 min), and the time until the end of the safe handling period (75 ± 12.3 min vs. 85 ± 6.8 min) were similar for both groups. Animals in both groups showed physiological stability during all evaluations, but hypoxemia was observed in one animal in each group. We conclude that both drug combinations are safe and effective at sedating red brocket deer in captivity and suggest that the procedure be performed with oxygen supplementation to reduce the potential for hypoxia., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2019
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44. NEOTROPICAL XENARTHRANS: a data set of occurrence of xenarthran species in the Neotropics.
- Author
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Santos PM, Bocchiglieri A, Chiarello AG, Paglia AP, Moreira A, de Souza AC, Abba AM, Paviolo A, Gatica A, Medeiro AZ, Costa AN, Gallina AG, Yanosky AA, Jesus A, Bertassoni A, Rocha A, Bovo AAA, Bager A, Mol AC, Martensen AC, Faustino AC, Lopes AMC, Percequillo AR, Vogliotti A, Keuroghlian A, de la Colina MA, Devlin AL, García-Olaechea A, Sánchez A, Srbek-Araujo AC, Ochoa AC, Oliveira ACM, Lacerda ACR, Campelo AKN, de Oliveira Paschoal AM, Costa ARC, Meiga AYY, Jesus AS, Feijó A, Hirsch A, da Silva ALF, Botelho ALM, Regolin AL, Lanna AM, Nunes AV, Kindel A, Moraes AM, Gatti A, Noss AJ, Nobre AB, Montanarin A, Deffaci ÂC, de Albuquerque ACF, de Oliveira AK, Mangione AM, Pontes ARM, Bertoldi AT, Calouro AM, Desbiez ALJ, Fernandes A, Ferreguetti AC, da Silva MAA, Zimbres B, Luciano BFL, de Thoisy B, Niebuhr BBS, Papi B, Gómez-Valencia B, Santos BA, Lima BC, Oliveira BG, Santos BS, Campos BATP, Leles B, de Albuquerque França BR, Lim B, Oliveira CT, Cantagallo C, Lara CC, Lima CS, Gestich CC, de Melo-Soares CD, Peres CA, Kasper CB, Candia-Gallardo C, De Angelo C, Fragoso CE, de Freitas CH, Salvador CH, Brocardo CR, Melo CD, Leuchtenberger C, Braga C, Sánchez-Lalinde C, Bueno C, Luna CL, Rojano C, Hurtado CM, Dos Santos CC, Tellaeche C, Rosa C, de Campos CB, Silva CR, Kanda CZ, Jenkins CN, McDonough C, Trinca CT, da Cunha CJ, Widmer CE, Santos C, Buscariol D, Carreira DC, Carvalho DR, da Silva Ferraz D, Casali D, Thornton D, Vasconcellos DR, Barcelos D, Brown D, Ramos DL, Moreira DO, Yogui DR, Faria D, Sana DA, de Mattia DL, Henz DJ, Friedeberg DB, Carvalho DLKP, Astúa D, Queirolo D, Varela DM, Eaton DP, Dias DM, Rivadeneira EF, Rocha EC, de Abreu-Júnior EF, Carrano E, Santos EM Jr, Setz EZF, Carvalho EAR Jr, de Almeida Chiquito E, de Matos Cardoso E, Mendonça EN, D'Bastiani E, Vieira EM, Ramalho EE, Guijosa-Guadarrama E, González E, Maggiorini EV, Fischer E, Aguiar EF, Castro ÉP, de la Peña-Cuéllar E, de Castro EBV, Brítez EB, Vanderhoeven EA, Pedó E, Rocha FL, Girardi F, de Oliveira Roque F, Mazim FD, de Barros FM, Martello F, Fantacini FM, Pedrosa F, Peters FB, Abra FD, de Azevedo FC, da Silva Santos F, da Silva FG, Teixeira FZ, Perini FA, Passos FC, Carvalho F, de Azevedo FCC, de Pinho FF, Gonçalves F, Lima F, Contreras-Moreno FM, Pedroni F, Tortato FR, Santos FPR, Caruso F, Tirelli FP, Miranda FR, Rodrigues FHG, Ubaid FK, Palmeira FBL, da Silva FA, Grotta-Neto F, de Souza FL, Costa FE, Pérez-Garduza F, Delsuc F, Lemos F, Pinto FR, Boaglio GI, Massocato GF, Preuss G, Hofmann GS, Aguiar GL, Oliveira GS, Duarte GT, Beca G, Giné GAF, Batista GO, Gil GE, Gonsioroski G, Secco H, Medeiros HR, Coelho IP, Franceschi IC, Bernardi I, de la Torre JA, Zocche JJ, Seibert JB, de Faria Falcão JC, Dias JHM, Nodari JZ, Oliveira JA, Giovanelli JGR, Favoretti JPP, Polisar J, Sponchiado J, Cherem JJ, Ramírez JFM, de Toledo JJ, Duarte JMB, de Matos JR, Arrabal JP, de Faria Oshima JE, Ribeiro JF, Bogoni JA, Pacheco JJC, Schuchmann KL, Ferraz KMPMB, Dos Santos Everton L, Bailey LL, Gonçalves LO, Cullen L Jr, de Andrade LR, Trevelin LC, Bonjorne L, de Almeida Rodrigues L, Leuzinger L, Perillo LN, Araújo LS, Hufnagel L, Ribeiro LO, Bernardo LRR, Oliveira-Santos LGR, Varzinczak LH, Borges LHM, Guimarães LN, Möcklinghoff L, Oliveira MA, Magioli M, de Assis Jardim MM, de Oliveira ML, Tortato MA, Dums M, Iezzi ME, Pereira MJR, Jorge ML, de Castro Morini MS, Landis MB, Xavier MS, Barros MAS, da Silva ML, Rivero M, Zanin M, Marques MI, Alves MH, Di Bitetti MS, Alvarez MR, Graipel ME, Godoi MN, Benedetti MA, Beltrão MG, Monteiro MCM, de Paula MJ, Perilli MLL, da Silva MP, Villar N, De Albuquerque NM, Canassa NF, Filho NM, da Rosa Oliveira N, Pasqualotto N, Cáceres NC, Attias N, Favarini MO, Ribeiro OS, Gonçalves PR, da Rocha PA, Condé PA, Akkawi P, Cruz P, Lira PK, Ferreira PM, Arroyo-Gerala P, Hartmann PA, de Tarso Zuquim Antas P, Marinho PH, de Faria Peres PH, Peña-Mondragón JL, Lombardi PM, de Souza Laurindo R, Alves RSC, Grangeiro RDP, Silva RL, Beltrão-Mendes R, Bonikowski RTR, Reppucci J, Arrais RC, Sampaio R, Sartorello R, Bovendorp RS, McNab R, Hack ROE, Magalhães RA, Araújo RC, de Almeida Nobre R, Pérez RRL, Massara RL, de Paula RC, Anleu RG, Marques RV, Dornas R, Rolim SG, Cavalcanti SMC, Lima SR, Ballari SA, Santamaría SB, Silva SM, Age SG, Godim T, Sobral-Souza T, Maccarini TB, Rodrigues TF, Piovezan U, Tavares VDC, Quiroga VA, Krepschi VG, Filho VP, Bastazini VAG, de Oliveira Gasparotto VP, Orsini VS, Layme VMG, Hannibal W, Dáttilo W, de Carvalho WD, Loughry WJ, Di Blanco YE, Núñez-Regueiro MM, Giubbina MF, Passamani M, de Alagão Querido LC, da Costa Toledo GA, Ribeiro IK, Quintilham L, de Bustos S, de la Maza J, Neto JFL, de Andrade Silva KVK, Sartorello L, Rampim LE, Marás GA, Camino M, Freitas-Junior M, Perovic PG, Paolino RM, Ferreira SD, Towns V, Esperandio IB, Aximoff I, Beduschi J, Guenther M, de Cassia Bianchi R, Keuroghlian-Eaton S, Mendes SL, de Fatima Cunha L, Cirignoli S, Ciocheti G, do Prado HA, Fernandes-Ferreira H, de Sena LMM, Yamane MH, Brennand PGG, da Silva RD, Escobar S, Endo W, Hurtado RR, Gontijo NRC, Marsh LK, Severo MM, Pardo JM, Costa SA, Melo GL, Santana GG, de Miranda Mourão G, Gaspari GG, Duarte H, Cabral H, da Silva LH, Mendonça L, Barbosa LL, Dos Santos MV, Moraes MFD, Gordo M, Versiani NF, Cantero N, Pays O, Guedes PG, Colas-Rosas PF, Ribeiro P, Renaud PC, Hoogesteijn RJ, Ayala R, da Cunha RGT, Schaub R, Laurito S, Betkowski SE, Cortez S, Silva SSP, de Oliveira TG, Spironello WR, Gengler N, Hidalgo MM, Juárez R, Iglesias JA, Anacleto TC, de Souza Fialho M, Cavicchioli G, Beccato MAB, Silva MD, Neto OC, Lopes KGD, Godoy LP, Luiz MR, Rojas Bonzi VB, Ferreira GB, Oliveira MJR, Hinojosa J, de Oliveira LFB, Nagy-Reis MB, Ramirez SF, Concone HVB, Mourthe I, Martínez-Lanfranco JA, Zanoni JB, Moreira TC, Guarderas ZV, Bazilio S, Cervini M, Pinheiro MS, Morato RG, Peroni N, Trigo TC, Machado RB, Gaspari F, Koenemann JG, Rudolf JC, Benchimol M, Vieira MV, Retta LM, Santiago PGF, Ciccia PG, Estrela PC, Carvalho S, Esbérard CEL, de la Cruz YB, Castro-Prieto J, Braga RM, Cartes JL, Andrade-Núñez MJ, Denkiewicz NM, Falconi N, Pezzuti JCB, Del Castillo Cordero HF, de Sousa LC, de Gaspari Júnior RL, Santos-Filho M, Almeida JS, Thompson JJ, Dos Santos JS, Pereira-Ribeiro J, Burs K, da Silva KFM, Velilla M, da Silva MX, de la Sancha NU, Pinheiro PF, de Castilho PV, Bercê W, Assis JC, Tonetti VR, Alves-Eigenheer M, Chinem S, Honda LK, de Godoy Bergallo H, Alberici V, Wallace R, Krauer JMC, Ribeiro MC, and Galetti M
- Abstract
Xenarthrans-anteaters, sloths, and armadillos-have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, 10 anteaters, and 6 sloths. Our data set includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae (3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data on Dasypus pilosus (Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized, but new genetic studies have revealed that the group is represented by seven species. In this data paper, we compiled a total of 42,528 records of 31 species, represented by occurrence and quantitative data, totaling 24,847 unique georeferenced records. The geographic range is from the southern United States, Mexico, and Caribbean countries at the northern portion of the Neotropics, to the austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regarding anteaters, Myrmecophaga tridactyla has the most records (n = 5,941), and Cyclopes sp. have the fewest (n = 240). The armadillo species with the most data is Dasypus novemcinctus (n = 11,588), and the fewest data are recorded for Calyptophractus retusus (n = 33). With regard to sloth species, Bradypus variegatus has the most records (n = 962), and Bradypus pygmaeus has the fewest (n = 12). Our main objective with Neotropical Xenarthrans is to make occurrence and quantitative data available to facilitate more ecological research, particularly if we integrate the xenarthran data with other data sets of Neotropical Series that will become available very soon (i.e., Neotropical Carnivores, Neotropical Invasive Mammals, and Neotropical Hunters and Dogs). Therefore, studies on trophic cascades, hunting pressure, habitat loss, fragmentation effects, species invasion, and climate change effects will be possible with the Neotropical Xenarthrans data set. Please cite this data paper when using its data in publications. We also request that researchers and teachers inform us of how they are using these data., (© 2019 The Authors. Ecology © 2019 The Ecological Society of America.)
- Published
- 2019
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45. Urofecal steroid profiles of captive Blue-fronted parrots (Amazona aestiva) with different reproductive outcomes.
- Author
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Pereira RJG, Christofoletti MD, Blank MH, and Duarte JMB
- Subjects
- Amazona metabolism, Animals, Animals, Zoo, Female, Gonadal Steroid Hormones metabolism, Immunoenzyme Techniques, Male, Parrots metabolism, Parrots physiology, Amazona physiology, Feces chemistry, Gonadal Steroid Hormones analysis, Reproduction physiology
- Abstract
Despite Psitaciformes (parrots) being the third largest nonpasserine order (398 species), it currently ranks second in number of threatened species (28%) according to the Internatinal Union for Conservation of Nature (IUCN) criteria. Since most of the literature concerning reproductive endocrinology in avian species derives from domestic and song birds, it is puzzling that advances in reproductive science for the Psitaciformes order lags far behind, in spite of the growing threats against them. In order to expand our knowledge of Neotropical parrots (Psittacidae), we examined annual changes in urofecal sex steroid metabolites of Blue-fronted amazon pairs (Amazona aestiva) exhibiting successful (nestlings) and unsuccessful breeding (infertile or no eggs). Urofecal samples were collected over a year from eight breeding pairs housed under the same environmental and management conditions. Fecal androgen and progestagen concentrations were determined in males and females, respectively, by enzyme immunoassays previously validated for this species. All eggs were registered between late winter and mid-spring, and egg-laying intervals varied between females (range: 1-8 days; average 3.60 ± 0.51 days). Similar profiles of urofecal progestagens were observed in reproductively successful females and females producing infertile eggs, with progestagen peaks preceding egg laying events (1.77 ± 0.50 days). In contrast, non-laying females had no rises in progestagens during the year. Successful and unsuccessful males did not displayed distinct annual patterns of androgen production, and apart from the peaks during the breeding season, more than half of the individuals intriguingly presented significant increases from late summer to early autumn, a period without reproductive activity. Finally, we noticed that samples with progestagen levels exceeding 40 ng/g had very high probability (>97.5%) to be from females in pre-laying or laying phases, suggesting a feasible application of this characteristic to noninvasively discriminate the reproductive status in amazon females with an accuracy and sensitivity of 94.55% and 58.13%, respectively. Our findings confirmed that urofecal progestagens and androgens are good indicators of the gonadal condition in Blue-fronted amazons, but there is still much to be done for their extensive use in artificial insemination or selection of the most suitable breeding birds for the season., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
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46. ATLANTIC MAMMAL TRAITS: a data set of morphological traits of mammals in the Atlantic Forest of South America.
- Author
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Gonçalves F, Bovendorp RS, Beca G, Bello C, Costa-Pereira R, Muylaert RL, Rodarte RR, Villar N, Souza R, Graipel ME, Cherem JJ, Faria D, Baumgarten J, Alvarez MR, Vieira EM, Cáceres N, Pardini R, Leite YLR, Costa LP, Mello MAR, Fischer E, Passos FC, Varzinczak LH, Prevedello JA, Cruz-Neto AP, Carvalho F, Percequillo AR, Paviolo A, Nava A, Duarte JMB, de la Sancha NU, Bernard E, Morato RG, Ribeiro JF, Becker RG, Paise G, Tomasi PS, Vélez-Garcia F, Melo GL, Sponchiado J, Cerezer F, Barros MAS, de Souza AQS, Dos Santos CC, Giné GAF, Kerches-Rogeri P, Weber MM, Ambar G, Cabrera-Martinez LV, Eriksson A, Silveira M, Santos CF, Alves L, Barbier E, Rezende GC, Garbino GST, Rios ÉO, Silva A, Nascimento ATA, de Carvalho RS, Feijó A, Arrabal J, Agostini I, Lamattina D, Costa S, Vanderhoeven E, de Melo FR, de Oliveira Laroque P, Jerusalinsky L, Valença-Montenegro MM, Martins AB, Ludwig G, de Azevedo RB, Anzóategui A, da Silva MX, Figuerêdo Duarte Moraes M, Vogliotti A, Gatti A, Püttker T, Barros CS, Martins TK, Keuroghlian A, Eaton DP, Neves CL, Nardi MS, Braga C, Gonçalves PR, Srbek-Araujo AC, Mendes P, de Oliveira JA, Soares FAM, Rocha PA, Crawshaw P Jr, Ribeiro MC, and Galetti M
- Abstract
Measures of traits are the basis of functional biological diversity. Numerous works consider mean species-level measures of traits while ignoring individual variance within species. However, there is a large amount of variation within species and it is increasingly apparent that it is important to consider trait variation not only between species, but also within species. Mammals are an interesting group for investigating trait-based approaches because they play diverse and important ecological functions (e.g., pollination, seed dispersal, predation, grazing) that are correlated with functional traits. Here we compile a data set comprising morphological and life history information of 279 mammal species from 39,850 individuals of 388 populations ranging from -5.83 to -29.75 decimal degrees of latitude and -34.82 to -56.73 decimal degrees of longitude in the Atlantic forest of South America. We present trait information from 16,840 individuals of 181 species of non-volant mammals (Rodentia, Didelphimorphia, Carnivora, Primates, Cingulata, Artiodactyla, Pilosa, Lagomorpha, Perissodactyla) and from 23,010 individuals of 98 species of volant mammals (Chiroptera). The traits reported include body mass, age, sex, reproductive stage, as well as the geographic coordinates of sampling for all taxa. Moreover, we gathered information on forearm length for bats and body length and tail length for rodents and marsupials. No copyright restrictions are associated with the use of this data set. Please cite this data paper when the data are used in publications. We also request that researchers and teachers inform us of how they are using the data., (© 2018 by the Ecological Society of America.)
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- 2018
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47. Monitoring ovarian cycles, pregnancy and post-partum in captive marsh deer ( Blastocerus dichotomus ) by measuring fecal steroids.
- Author
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Polegato BF, Zanetti EDS, and Duarte JMB
- Abstract
The marsh deer is an endangered species from the marshlands of central South America. This study aimed to characterize certain aspects of the reproductive physiology of marsh deer hinds, including the duration and fecal progestins profile of the estrous cycle, pregnancy and post-partum periods, and evaluate the effect of cloprostenol administration on this species. The experimental group consisted of six females and one fertile male marsh deer. During monitoring of the estrous cycle, the fresh fecal samples were collected daily and, during pregnancy, they were collected twice weekly. The hormonal profile obtained from daily fecal samples indicated that the mean duration of the estrous cycle was 21.3 ± 1.3 days (6.4 days inter-luteal phase and 14.8 days luteal phase; n = 16 estrous cycles). The mean concentration of fecal progestins in the inter-luteal phase was 834 ± 311 ng g
-1 , in the luteal phase was 3979 ± 1611 ng g-1 , value between them was 1457 ng g-1 . No significant difference in fecal estrogen concentrations was determined during the estrous cycle. The corpora luteum was not responsive to cloprostenol until Day 6 of the estrous cycle, the period previously described as the inter-luteal phase. Half the females became pregnant following treatment with cloprostenol and two others were fertilized in their natural estrous cycle. Four females delivered fawns, and the mean duration of pregnancy was 253 ± 4 days. Fecal progestin concentrations were similar to those of the estrous cycle during the first 11 weeks of pregnancy and increased significantly ( > 15250 ng g-1 ) thereafter, providing a presumptive diagnosis guideline. Within 60 days of post-partum analyses, 75% of the deer exhibited behavioural estrus and/or ovarian activity. This study generated a broader understanding of the marsh deer species concerning the production of consistent data related to its reproduction. This knowledge can be used to assist the reproductive management of this species and, consequently, to promote its conservation.- Published
- 2018
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48. Intrapopulation Chromosomal Polymorphism in Mazama gouazoubira (Cetartiodactyla; Cervidae): The Emergence of a New Species?
- Author
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Valeri MP, Tomazella IM, and Duarte JMB
- Subjects
- Animals, Chromosomes, Mammalian, Female, Karyotype, Male, Deer genetics, Polymorphism, Genetic
- Abstract
Mazama gouazoubira is a small deer species widely distributed in South America. Previous studies have shown that this species presents intraspecific chromosomal polymorphisms, which could affect fertility due to the effects of chromosomal rearrangements on gamete formation. Important aspects regarding the karyotype evolution of this species and the genus remain undefined due to the lack of information concerning the causes of this chromosomal variation. Nineteen individuals belonging to the Mazama gouazoubira population located in the Pantanal were cytogenetically evaluated. Among the individuals analyzed, 9 had B chromosomes and 5 carried a heterozygous centric fusion (2n = 69 and FN = 70). In 3 individuals, the fusion occurred between chromosomes X and 16, in 1 individual between chromosomes 7 and 21, and in another individual between chromosomes 4 and 16. These striking polymorphisms could be explained by several hypotheses. One is that the chromosome rearrangements in this species are recent and not fixed in the population yet, and another hypothesis is that they represent a balanced polymorphism and that heterozygotes have an adaptive advantage. On the other hand, these polymorphisms may negatively influence fertility and raise questions about sustainability or reproductive isolation of the population., (© 2018 S. Karger AG, Basel.)
- Published
- 2018
- Full Text
- View/download PDF
49. The Effects of Cryopreservation on Different Passages of Fibroblast Cell Culture in Brown Brocket Deer (Mazama gouazoubira).
- Author
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Magalhães LC, Bhat MH, Freitas JLS, Melo LM, Teixeira DIA, Pinto LCA, Câmara LMC, Duarte JMB, and Freitas VJF
- Subjects
- Animals, Apoptosis, Cell Survival, Cells, Cultured, Cryopreservation instrumentation, Deer, Cryopreservation methods, Fibroblasts cytology
- Abstract
The brown brocket deer Mazama gouazoubira is 1 of the 10 recognized brocket deer of the Neotropical region. Recently, this species has suffered a population decline due to current threats, mainly poaching and habitat loss. Several studies have shown that some endangered species can benefit from interspecies somatic cell nuclear transfer technology through the use of their somatic cells, such as the fibroblasts. Thus, the aim of this study was to verify the viability and the effect of cryopreservation on fibroblasts after several passages. For this purpose, fibroblast cells were cultured until passages 4, 7, and 10 (cultured control groups) and cryopreserved in cryotubes (frozen/warmed groups). The cellular viability, functionality, and percentage of cells undergoing necrosis and apoptosis were evaluated. The survival rates were always higher than 80% irrespective of the tested group, except for passage 10 in the frozen/warmed group. Population doubling time of cultured cells from passage 10 was significantly higher than that of passages 4 and 7, exhibiting low metabolic activity and a higher percentage of cells in initial apoptosis. In conclusion, the M. gouazoubira fibroblast-derived cell line provides an essential resource for further studies regarding reproductive biotechniques and is likely to be useful as an ex situ conservation strategy.
- Published
- 2017
- Full Text
- View/download PDF
50. Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
- Author
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Mantellatto AMB, Caparroz R, Christofoletti MD, Piovezan U, and Duarte JMB
- Abstract
The pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.
- Published
- 2017
- Full Text
- View/download PDF
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