31 results on '"Dubicke, A."'
Search Results
2. Density of Stromal Cells and Macrophages Associated With Collagen Remodeling in the Human Cervix in Preterm and Term Birth
- Author
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Dubicke, Aurelija, Ekman-Ordeberg, Gunvor, Mazurek, Patricia, Miller, Lindsay, and Yellon, Steven M.
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- 2016
- Full Text
- View/download PDF
3. The preterm cervix reveals a transcriptomic signature in the presence of premature prelabor rupture of membranes
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Makieva, Sofia, Dubicke, Aurelija, Rinaldi, Sara F., Fransson, Emma, Ekman-Ordeberg, Gunvor, and Norman, Jane E.
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- 2017
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4. Pro-inflammatory and anti-inflammatory cytokines in human preterm and term cervical ripening
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Dubicke, Aurelija, Fransson, Emma, Centini, Gabriele, Andersson, Eva, Byström, Birgitta, Malmström, Anders, Petraglia, Felice, Sverremark-Ekström, Eva, and Ekman-Ordeberg, Gunvor
- Published
- 2010
- Full Text
- View/download PDF
5. High-mobility group box protein 1 and its signalling receptors in human preterm and term cervix
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Dubicke, Aurelija, Andersson, Peter, Fransson, Emma, Andersson, Eva, Sioutas, Angelos, Malmström, Anders, Sverremark-Ekström, Eva, and Ekman-Ordeberg, Gunvor
- Published
- 2010
- Full Text
- View/download PDF
6. Low levels of anti-secretory factor in placenta are associated with preterm birth and inflammation
- Author
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Stefan Lange, Aurelija Dubicke, Anna Hjelmstedt, Eva Jennische, Emma Fransson, Anna Gustafsson, Kajsa Bohlin, Sven Arne Silfverdal, and Gunvor Ekman-Ordeberg
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Adult ,medicine.medical_specialty ,Adolescent ,Placenta ,Inflammation ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Internal medicine ,medicine ,Humans ,Longitudinal Studies ,Prospective Studies ,030219 obstetrics & reproductive medicine ,business.industry ,Anti secretory ,Neuropeptides ,Obstetrics and Gynecology ,A protein ,General Medicine ,Endocrinology ,medicine.anatomical_structure ,Premature Birth ,Female ,medicine.symptom ,business ,Biomarkers ,030215 immunology - Abstract
Anti-secretory factor is a protein that regulates secretory and inflammatory processes and preterm birth is associated with inflammation. Therefore, our hypothesis was that anti-secretory factor might play a role in immune reactivity and homeostasis during pregnancy.Following spontaneous onset of labor and preterm or term delivery, placenta biopsies were collected. The levels of anti-secretory factor and markers of inflammation (CD68, CD163) and vascularization (CD34, smooth muscle actin) were analyzed by immunohistochemistry.The 61 placental biopsies included 31 preterm (37 weeks of gestation) and 30 term (37-41 weeks) samples. The preterm placentas exhibited lower levels of anti-secretory factor (p = 0.008) and larger numbers of CD68-positive cells (p0.001) compared to term. Preterm placentas had blood vessel of smaller diameter (p = 0.036) indicative of immaturity. The level of interleukin-6 in cord blood was higher after very preterm than term birth, suggesting a fetal inflammatory response. The placenta level of anti-secretory factor was positively correlated to the length of gestation (p = 0.025) and negatively correlated to the levels of the inflammatory markers CD68 (p = 0.015) and CD163 (p = 0.028).Preterm delivery is associated with low levels of anti-secretory factor in placenta. Inflammation, a potential trigger of preterm birth, is more pronounced in the preterm placenta and inversely related to the placental level of anti-secretory factor, suggesting both a link and a potential target for intervention.
- Published
- 2018
7. Negative Emotions and Cytokines in Maternal and Cord Serum at Preterm Birth
- Author
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Fransson, Emma, Dubicke, Aurelija, Byström, Birgitta, Ekman-Ordeberg, Gunvor, Hjelmstedt, Anna, and Lekander, Mats
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- 2012
- Full Text
- View/download PDF
8. Does low molecular weight heparin shorten term labor?
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EKMAN-ORDEBERG, GUNVOR, ÅKERUD, ANNA, DUBICKE, AURELIJA, MALMSTRÖM, ANDERS, and HELLGREN, MARGARETA
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- 2010
- Full Text
- View/download PDF
9. Low molecular weight heparin stimulates myometrial contractility and cervical remodeling in vitro
- Author
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EKMAN-ORDEBERG, GUNVOR, HELLGREN, MARGARETA, ÅKERUD, ANNA, ANDERSSON, EVA, DUBICKE, AURELIJA, SENNSTRÖM, MARIA, BYSTRÖM, BIRGITTA, TZORTZATOS, GERASIMOS, GOMEZ, MARIA F., EDLUND, MÅNS, LINDAHL, ULF, and MALMSTRÖM, ANDERS
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- 2009
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10. Different secretion patterns of matrix metalloproteinases and IL-8 and effect of corticotropin-releasing hormone in preterm and term cervical fibroblasts
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Dubicke, A., Akerud, A., Sennstrom, M., Hamad, R. Rafik, Bystrom, B., Malmstrom, A., and Ekman-Ordeberg, G.
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- 2008
11. Differences in heparan sulfate production in cervical fibroblast cultures from women undergoing term and preterm delivery
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ÅKERUD, ANNA, DUBICKE, AURELIJA, SENNSTRÖM, MARIA, EKMAN-ORDEBERG, GUNVOR, and MALMSTRÖM, ANDERS
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- 2008
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12. The preterm cervix reveals a transcriptomic signature in the presence of premature prelabor rupture of membranes
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Sofia, Makieva, Aurelija, Dubicke, Sara F, Rinaldi, Emma, Fransson, Gunvor, Ekman-Ordeberg, and Jane E, Norman
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Fetal Membranes, Premature Rupture ,Labor, Obstetric ,Reverse Transcriptase Polymerase Chain Reaction ,Biopsy ,Tumor Suppressor Proteins ,Protein Array Analysis ,Cervix Uteri ,Carcinoembryonic Antigen ,Gene Expression Regulation ,Matrix Metalloproteinase 9 ,Pregnancy ,Guanine Nucleotide Exchange Factors ,Humans ,Matrix Metalloproteinase 2 ,Female ,Transcriptome ,Adaptor Proteins, Signal Transducing - Abstract
Premature prelabor rupture of fetal membranes accounts for 30% of all premature births and is associated with detrimental long-term infant outcomes. Premature cervical remodeling, facilitated by matrix metalloproteinases, may trigger rupture at the zone of the fetal membranes overlying the cervix. The similarities and differences underlying cervical remodeling in premature prelabor rupture of fetal membranes and spontaneous preterm labor with intact membranes are unexplored.We aimed to perform the first transcriptomic assessment of the preterm human cervix to identify differences between premature prelabor rupture of fetal membranes and preterm labor with intact membranes and to compare the enzymatic activities of matrix metalloproteinases-2 and -9 between premature prelabor rupture of fetal membranes and preterm labor with intact membranes.Cervical biopsies were collected following preterm labor with intact membranes (n = 6) and premature prelabor rupture of fetal membranes (n = 5). Biopsies were also collected from reference groups at term labor (n = 12) or term not labor (n = 5). The Illumina HT-12 version 4.0 BeadChips microarray was utilized, and a novel network graph approach determined the specificity of changes between premature prelabor rupture of fetal membranes and preterm labor with intact membranes. Quantitative reverse transcription-polymerase chain reaction and Western blotting confirmed the microarray findings. Immunofluorescence was used for localization studies and gelatin zymography to assess matrix metalloproteinase activity.PML-RARA-regulated adapter molecule 1, FYVE-RhoGEF and PH domain-containing protein 3 and carcinoembryonic antigen-ralated cell adhesion molecule 3 were significantly higher, whereas N-myc downstream regulated gene 2 was lower in the premature prelabor rupture of fetal membranes cervix when compared with the cervix in preterm labor with intact membranes, term labor, and term not labor. PRAM1 and CEACAM3 were localized to immune cells at the cervical stroma and NDRG2 and FGD3 were localized to cervical myofibroblasts. The activity of matrix metalloproteinase-9 was higher (1.22 ± 4.403-fold, P.05) in the cervix in premature prelabor rupture of fetal membranes compared with preterm labor with intact membranes.We identified 4 novel proteins with a potential role in the regulation of cervical remodeling leading to premature prelabor rupture of fetal membranes. Our findings contribute to the studies dissecting the mechanisms underlying premature prelabor rupture of fetal membranes and inspire further investigations toward the development of premature prelabor rupture of fetal membranes therapeutics.
- Published
- 2016
13. Density of Stromal Cells and Macrophages Associated With Collagen Remodeling in the Human Cervix in Preterm and Term Birth
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Aurelija Dubicke, Gunvor Ekman-Ordeberg, Patricia Mazurek, Lindsay Miller, and Steven M. Yellon
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0301 basic medicine ,Adult ,medicine.medical_specialty ,Stromal cell ,Adolescent ,Term Birth ,Inflammation ,Cell Count ,Cervix Uteri ,Andrology ,03 medical and health sciences ,Hydroxyproline ,chemistry.chemical_compound ,Young Adult ,0302 clinical medicine ,Stroma ,Pregnancy ,medicine ,Humans ,RNA, Messenger ,Cervix ,030219 obstetrics & reproductive medicine ,Obstetrics ,business.industry ,Macrophages ,Obstetrics and Gynecology ,Original Articles ,medicine.disease ,female genital diseases and pregnancy complications ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Premature birth ,Gestation ,Premature Birth ,Female ,Collagen ,medicine.symptom ,Stromal Cells ,business - Abstract
Remodeling of the cervix occurs in advance of labor both at term and at preterm birth. Morphological characteristics associated with remodeling in rodents were assessed in cervix biopsies from women at term (39 weeks' gestation) and preterm (
- Published
- 2015
14. Negative Emotions and Cytokines in Maternal and Cord Serum at Preterm Birth
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Birgitta Byström, Gunvor Ekman-Ordeberg, Aurelija Dubicke, Mats Lekander, Emma Fransson, and Anna Hjelmstedt
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medicine.medical_specialty ,Pregnancy ,Obstetrics ,business.industry ,Immunology ,Obstetrics and Gynecology ,medicine.disease ,Reproductive Medicine ,Premature birth ,CORD SERUM ,Cord blood ,medicine ,Immunology and Allergy ,Young adult ,business ,Depression (differential diagnoses) ,Psychoneuroimmunology ,Umbilical Cord Serum - Abstract
Problem This study investigates whether affectivity differs between mothers delivering preterm and term and whether maternal and umbilical cord serum cytokines differ between these groups. Further, ...
- Published
- 2011
15. Different secretion patterns of matrix metalloproteinases and IL-8 and effect of corticotropin-releasing hormone in preterm and term cervical fibroblasts
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R. Rafik Hamad, Anders Malmström, Anna Åkerud, Aurelija Dubicke, Gunvor Ekman-Ordeberg, Maria Sennström, and Birgitta Byström
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Adult ,Embryology ,medicine.medical_specialty ,Adolescent ,Corticotropin-Releasing Hormone ,Term Birth ,extracellular matrix ,Cervix Uteri ,Biology ,Matrix metalloproteinase ,Gene Expression Regulation, Enzymologic ,Corticotropin-releasing hormone ,Pregnancy ,Internal medicine ,Genetics ,medicine ,Humans ,cervical ripening ,Secretion ,RNA, Messenger ,Interleukin 8 ,Molecular Biology ,Cells, Cultured ,Interleukin-8 ,matrix metalloproteinases ,preterm birth ,Obstetrics and Gynecology ,Interleukin ,Articles ,Cell Biology ,Fibroblasts ,medicine.disease ,cytokines ,Endocrinology ,Reproductive Medicine ,Premature birth ,Premature Birth ,Female ,Biomarkers ,Developmental Biology ,Hormone - Abstract
The aims of the present study were to compare the levels of mRNA and protein expression of matrix metalloproteinase (MMP)-1, -3, -8 and -9 in human cervical tissue in preterm and term labor as well as not in labor and to determine if corticotropin-releasing hormone (CRH) has an effect on MMP-1, -3 and interleukin (IL)-8 secretion in both preterm and term cervical fibroblasts. Cervical biopsies were taken from 60 women: 18 at preterm labor, 7 at preterm not in labor, 18 at term labor and 17 at term not in labor. ELISA and Immulite were used for protein and real-time RT-PCR for mRNA analysis. Cervical fibroblast cultures were incubated for 18 h with different CRH concentrations (10(-13)-10(-6) M). The mRNA expression of MMP-1, -3 and -9 was higher in laboring groups compared with term not in labor. Protein levels of MMP-8 and -9 were higher in term in labor group compared with non-laboring groups. There were no significant differences in mRNA and protein expression between the preterm and respective term control groups. CRH significantly increased secretion of IL-8 in preterm and term cervical fibroblasts compared with controls. The secretion of IL-8 and MMP-1 was significantly higher and MMP-3 secretion lower in preterm cervical fibroblasts. In conclusion, cervical ripening at preterm seems to be a similar inflammatory process as at term with CRH involved. However, preterm and term cervical fibroblasts might have different phenotypes based on different secretion patterns of IL-8, MMP-1 and MMP-3.
- Published
- 2008
16. Abstract # 1698 Childhood experience of parental separation and associations with perinatal inflammation
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Fransson, E., primary, Dubicke, A., additional, and Lekander, M., additional
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- 2016
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17. Preterm Cervical Ripening in humans
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G, Ekman-Ordeberg and A, Dubicke
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HMGB1 ,CRH ,Cervical ripening ,food and beverages ,Review ,preterm labour ,cytokines ,cervical fibroblasts - Abstract
Preterm birth (PTB) is the leading cause of neonatal mortality and morbidity. Despite the current treatment procedures, the incidence of PTB has not changed in the past thirty years. Incomplete understanding of the biological and patophysiological mechanisms underlying preterm delivery is the major obstacle to prevent PTB. Cervical ripening is necessary for vaginal delivery and understanding of preterm cervical ripening is required for developing new treatment strategies. Several important substances such as HMGB1 and its receptors, CRH and its receptors and numerous cytokines are localized in the cervix and undergo distinct changes in labour. Other important -molecules, such as CRH, CRH-BP, CRH-R1, CRH-R2, HMGB1, TLR2, TLR4, IL-10, IL-12, are localized in the cervical epithelium, also indicating their role in the process of cervical ripening during labour. Furthermore, CRH stimulates IL-8 secretion from both preterm and term cervical fibroblasts. Recent studies from our group show that major -inflammatory changes occur in the cervix at labour irrespective of gestational age. This indicates that cervical ripening at both term and preterm is an inflammatory process even if no infection is present. However, preterm cervical ripening still entails some differences from term cervical ripening, for example in the down-regulation of mRNA expression of Toll-like receptors (TLR-2 and TLR-4) and IL-12, higher levels of IL-10 in cervical epithelium, and presents different secretion patterns of cervical fibroblasts. Moreover, preterm cervical ripening, like preterm delivery itself, is a multifactorial disorder with pathways which are partly different from those involved in PPROM and infected preterm labour.
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- 2014
18. Abstract # 1698 Childhood experience of parental separation and associations with perinatal inflammation
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Aurelija Dubicke, Emma Fransson, and Mats Lekander
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Pediatrics ,medicine.medical_specialty ,Pregnancy ,Endocrine and Autonomic Systems ,Offspring ,business.industry ,Immunology ,Interleukin ,Inflammation ,Disease ,Affect (psychology) ,medicine.disease ,Parental separation ,Behavioral Neuroscience ,Cord blood ,medicine ,medicine.symptom ,business - Abstract
Adversities in childhood are suggested to affect neuroendocrine pathways that in turn increases the risk of elevated inflammation and inflammatory disease in adulthood. During pregnancy, maternal immune activation has been linked with poor pregnancy outcome and risk for neurodevelopmental disease in the offspring. This study investigates childhood experience of parental separation and current socio economic position in relation to adult immune reaction at the event of giving birth, term or preterm. Sixty-four women were sampled during delivery and post-partum. Serum levels of Interleukin (IL)-6 were analyzed from maternal (preterm n = 27, term n = 37) and cord blood (preterm n = 23, term n = 32). Information on annual income and childhood family situation was collected through questionnaires. Multiple linear regression analyses using log transformed IL-6 data, showed associations between childhood experience of parental separations and elevated levels of maternal IL-6 from labor and post-partum, p p
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- 2016
19. Low levels of anti-secretory factor in placenta are associated with preterm birth and inflammation.
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Bohlin, Kajsa, Gustafsson, Anna M., Fransson, Emma, Dubicke, Aurelija, Hjelmstedt, Anna K., Ekman‐Ordeberg, Gunvor, Silfverdal, Sven‐Arne, Lange, Stefan, Jennische, Eva, Ekman-Ordeberg, Gunvor, and Silfverdal, Sven-Arne
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PREMATURE labor ,INTERLEUKIN receptors ,PLACENTA ,VASCULAR diseases ,IMMUNOHISTOCHEMISTRY - Abstract
Introduction: Anti-secretory factor is a protein that regulates secretory and inflammatory processes and preterm birth is associated with inflammation. Therefore, our hypothesis was that anti-secretory factor might play a role in immune reactivity and homeostasis during pregnancy.Material and Methods: Following spontaneous onset of labor and preterm or term delivery, placenta biopsies were collected. The levels of anti-secretory factor and markers of inflammation (CD68, CD163) and vascularization (CD34, smooth muscle actin) were analyzed by immunohistochemistry.Results: The 61 placental biopsies included 31 preterm (<37 weeks of gestation) and 30 term (37-41 weeks) samples. The preterm placentas exhibited lower levels of anti-secretory factor (p = 0.008) and larger numbers of CD68-positive cells (p < 0.001) compared to term. Preterm placentas had blood vessel of smaller diameter (p = 0.036) indicative of immaturity. The level of interleukin-6 in cord blood was higher after very preterm than term birth, suggesting a fetal inflammatory response. The placenta level of anti-secretory factor was positively correlated to the length of gestation (p = 0.025) and negatively correlated to the levels of the inflammatory markers CD68 (p = 0.015) and CD163 (p = 0.028).Conclusions: Preterm delivery is associated with low levels of anti-secretory factor in placenta. Inflammation, a potential trigger of preterm birth, is more pronounced in the preterm placenta and inversely related to the placental level of anti-secretory factor, suggesting both a link and a potential target for intervention. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
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20. Negative emotions and cytokines in maternal and cord serum at preterm birth
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Emma, Fransson, Aurelija, Dubicke, Birgitta, Byström, Gunvor, Ekman-Ordeberg, Anna, Hjelmstedt, and Mats, Lekander
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Adult ,Male ,Young Adult ,Depression ,Pregnancy ,Emotions ,Cytokines ,Humans ,Premature Birth ,Female ,Fetal Blood - Abstract
This study investigates whether affectivity differs between mothers delivering preterm and term and whether maternal and umbilical cord serum cytokines differ between these groups. Further, whether there are associations between mothers' emotions and maternal and cord cytokines at preterm and term birth.Twenty-seven mothers delivering preterm and 37 mothers delivering at term reported positive/negative affect and previous depressive symptoms during pregnancy. Blood samples from mothers in labor and cord samples (23 preterm and 33 term) were analyzed for cytokines.Maternal IL-8 was lower at preterm delivery compared with term. In the preterm group only, associations were found between negative emotions and maternal IL-6, IL-8 and cord IL-6, IL-8, IL-10, IL-13, and IL-18.The findings indicate associations in preterm delivery between negative emotions and both maternal and neonate immune activity. Future studies should investigate whether such associations are part of the etiology of preterm delivery.
- Published
- 2011
21. High-mobility group box protein 1 and its signalling receptors in human preterm and term cervix
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Anders Malmström, Eva Andersson, Aurelija Dubicke, Emma Fransson, Gunvor Ekman-Ordeberg, Angelos Sioutas, Peter Andersson, and Eva Sverremark-Ekström
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Adult ,medicine.medical_specialty ,Receptor for Advanced Glycation End Products ,Immunology ,chemical and pharmacologic phenomena ,Cervix Uteri ,Biology ,HMGB1 ,Young Adult ,Obstetric Labor, Premature ,Pregnancy ,Internal medicine ,Naturvetenskap ,medicine ,Humans ,Immunology and Allergy ,RNA, Messenger ,HMGB1 Protein ,Receptors, Immunologic ,Receptor ,Cervix ,reproductive and urinary physiology ,Messenger RNA ,Labor, Obstetric ,Obstetrics and Gynecology ,Middle Aged ,medicine.disease ,Toll-Like Receptor 2 ,Toll-Like Receptor 4 ,TLR2 ,medicine.anatomical_structure ,Endocrinology ,Reproductive Medicine ,biology.protein ,Female ,Signal transduction ,Natural Sciences ,Premature rupture of membranes ,Cervical Ripening ,Signal Transduction - Abstract
The objective of this study was to identify possible changes in mRNA and protein expression of high-mobility group box protein 1 (HMGB1) and its suggested receptors - receptor for advanced glycation end-products (RAGE) and Toll-like receptor 2 (TLR2) and TLR4 - in human cervix during pregnancy, term and preterm labor. Cervical biopsies were taken from 58 women: 20 at preterm labor, 24 at term labor, 10 at term not in labor and 4 from non-pregnant women. Real-time RT-PCR was used to quantify mRNA expression, and immunohistochemistry and ELISA for protein analysis. HMGB1, RAGE, TLR2 and TLR4 proteins were localized and their mRNA expression was detected in the cervix. There was more extranuclear HMGB1 in the cervical epithelium and stroma in preterm and term labor compared to the term not in labor. TLR2 mRNA expression was upregulated 5-fold in term labor and 3-fold in preterm labor compared to term not in labor and non-pregnant controls. There was lower expression of TLR2 and TLR4 mRNAs in preterm labor compared to term. Lower mRNA expression of HMGB1 was found in the subgroup with preterm premature rupture of membranes than in the rest of the preterm group, where levels were significantly higher than in term labor. In conclusion, extranuclear expression of HMGB1 during labor suggests a possible role of HMGB1 during the process of cervical ripening. Changes in expression of mRNAs encoding HMGB1, TLR2 and TLR4 in preterm labor suggest differences in the mechanism of cervical ripening at preterm and term delivery. 8
- Published
- 2010
22. Low molecular weight heparin stimulates myometrial contractility and cervical remodeling in vitro
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Anders Malmström, Aurelija Dubicke, Anna Åkerud, Måns Edlund, Eva Andersson, Birgitta Byström, Maria F. Gomez, Ulf Lindahl, Maria Sennström, Gunvor Ekman-Ordeberg, Gerasimos Tzortzatos, and Margareta Hellgren
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Adult ,Dalteparin ,medicine.medical_specialty ,medicine.drug_class ,Cell Culture Techniques ,Low molecular weight heparin ,Cervix Uteri ,Contractility ,Tissue Culture Techniques ,Uterine Contraction ,In vivo ,Pregnancy ,Internal medicine ,Medicine ,Humans ,Secretion ,Fibroblast ,business.industry ,Anticoagulant ,Interleukin-8 ,Myometrium ,Obstetrics and Gynecology ,Anticoagulants ,General Medicine ,Fibroblasts ,In vitro ,Endocrinology ,medicine.anatomical_structure ,Female ,business ,Cervical Ripening - Abstract
Objectives. The low molecular weight heparin, Dalteparin, shortens human labor time. The aim of this study was to investigate if the mechanism behind this effect involves myometrial contractility and cervical ripening and if the anticoagulative activity is necessary for its effect. Design. Experimental in vitro study. Setting. Lund University and Karolinska Institute, Sweden. Methods. The effect of low molecular weight heparins with or without anticoagulative properties on myometrial contractility was measured in vitro on smooth muscle strips from biopsies obtained at elective cesarean sections. The effects on cervical ripening were assessed in cervical fibroblasts cultured from explants of cervical biopsies obtained at delivery. Main outcome measures. Mean force and number of contractions in uterine smooth muscle strips and interleukin-8 (IL-8) secretion in cervical fibroblasts. Results. Myometrial smooth muscle strips pretreated with low molecular weight heparins showed increased contractile activity compared to untreated smooth muscle strips. Secretion of IL-8 from cultured cervical fibroblasts was significantly increased after treatment with low molecular weight heparin. Both these effects were independent of anticoagulative activity of the low molecular weight heparin. Conclusions. A possible underlying mechanism for the shortened labor time after low molecular weight heparin treatment is enhanced myometrial contractility and an increased IL-8 secretion in cervical fibroblast, mimicking the final cervical ripening in vivo. Our data support the notion that anticoagulant activity is not required to promote labor.
- Published
- 2009
23. Pro-inflammatory and anti-inflammatory cytokines in human preterm and term cervical ripening
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Birgitta Byström, Eva Sverremark-Ekström, Aurelija Dubicke, Gunvor Ekman-Ordeberg, Gabriele Centini, Felice Petraglia, Anders Malmström, Eva Andersson, and Emma Fransson
- Subjects
Adult ,medicine.medical_treatment ,Biopsy ,Immunology ,Inflammation ,Cervix Uteri ,Cervix ,Andrology ,Pathogenesis ,Obstetric Labor, Premature ,Downregulation and upregulation ,Pregnancy ,medicine ,Immunology and Allergy ,Humans ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,Cervical ripening ,Cytokines ,Preterm birth ,Immunology in the medical area ,Obstetrics and Gynecology ,Interleukin ,Bacterial Infections ,Middle Aged ,medicine.disease ,Immunohistochemistry ,medicine.anatomical_structure ,Cytokine ,Reproductive Medicine ,Gene Expression Regulation ,Immunologi inom det medicinska området ,Immunologi ,Gestation ,Female ,medicine.symptom ,business ,Cervical Ripening - Abstract
Cervical ripening is necessary for successful delivery. Since cytokines are believed to be involved in this process, the aim of this study was to investigate possible changes in the mRNA and protein expression of pro-inflammatory cytokines (interleukin (IL)-1 alpha, IL-1 beta, IL-12, IL-18) and anti-inflammatory cytokines (IL-4, IL-10, IL-13)in the human cervix during pregnancy, term and preterm labor. Cervical biopsies were taken from 59 women: 21 at preterm labor, 24 at term labor, 10 at term not in labor and 4 from non-pregnant women. mRNA was analyzed with real-time RT-PCR and protein expression and/or secretion with immunohistochemistry and ELISA. There was an upregulation of mRNA for IL-10, IL-13, IL-1 alpha and IL-1 beta in the laboring groups, while mRNA for IL-12 and IL-18 was downregulated. IL-4 mRNA was detected more frequently, while IL-12 mRNA expression was lower, in the preterm labor group than in the term labor group. The protein levels of IL-4 and IL-12 were lower and IL-18 tended to be higher in the labor groups, while IL-10 protein levels were unaffected by labor. IL-4 protein levels were significantly higher in the preterm subgroup with bacterial infection than in the non-infected group. IL-10 had higher expression in squamous epithelium at preterm labor than at term. In conclusion, the major changes in pro-inflammatory and anti-inflammatory cytokine mRNA and protein expression in cervix occur during the labor process irrespective of the length of gestation. Our results indicate that dysregulation of anti-inflammatory cytokines in the human cervix could be involved in the pathogenesis of preterm labor.
- Published
- 2009
24. Does Low Molecular Weight Heparin Shorten Term Labor?
- Author
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Aurelija Dubicke, Gunvor Ekman-Ordeberg, Margareta Hellgren, Anna Åkerud, and Anders Malmström
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Adult ,Anesthesia, Epidural ,Dalteparin ,medicine.medical_specialty ,Time Factors ,Adolescent ,Preterm labor ,medicine.drug_class ,Psychological intervention ,Low molecular weight heparin ,Thrombophilia ,Heparan Sulfate Proteoglycans ,Young Adult ,Pregnancy ,medicine ,Anesthesia, Obstetrical ,Humans ,Young adult ,reproductive and urinary physiology ,Retrospective Studies ,Gynecology ,Labor, Obstetric ,Dalteparin sodium ,Obstetrics ,business.industry ,Anticoagulant ,Parturition ,Pregnancy Outcome ,During current pregnancy ,Anticoagulants ,Obstetrics and Gynecology ,Retrospective cohort study ,General Medicine ,medicine.disease ,Apgar Score ,Female ,Apgar score ,business ,Venous thromboembolism ,medicine.drug - Abstract
Clinical experience has fostered the belief among some clinicians that women who receive low molecular weight heparins (LMWHs) are less likely to have protracted labor and therefore, less likely to require operative delivery. Previous studies have identified changes in the uterine and cervical extracellular matrix of heparan sulfate proteoglycans in normal and protracted labor. These changes and clinical observations suggest that LMWHs may influence the parturition process and shorten delivery time. This retrospective study investigated the effects of LMWHs on parturition time in pregnant women with a history of thrombotic disorders. The study subjects were 99 nulliparous pregnant women who had been treated with an LMWH (dalteparin) because of a history of previous venous thromboembolism, thrombophilia, or an acute venous thromboembolism during the current pregnancy. The women had been treated for 1 to 6 months before delivery. The control group was comprised of 198 untreated healthy nulliparous women with spontaneous onset of labor matched according to age and parity. The study and control groups were compared with respect to labor time, proportion of operative deliveries, and neonatal outcomes. Among women who had a spontaneous vaginal delivery, labor time was significantly shorter in those treated with dalteparin than in the untreated controls (6 vs. 9 hours, P < 0.0001). The number of operative deliveries was the same in the dalteparin and control groups, but the dalteparin-treated women required fewer operative interventions due to protracted labor (P = 0.0014). There was no difference in neonatal outcome between the groups. These findings suggest that LMWH therapy is associated with shorter labor time and a decreased number of operative interventions for protracted labor.
- Published
- 2010
25. 4. Stressful life events during pregnancy might modulate local inflammation during delivery
- Author
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Fransson, E., primary, Dubicke, A., additional, Ekman-Ordeberg, G., additional, and Hjelmstedt, A., additional
- Published
- 2012
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26. Negative Emotions and Cytokines in Maternal and Cord Serum at Preterm Birth
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Fransson, Emma, primary, Dubicke, Aurelija, additional, Byström, Birgitta, additional, Ekman‐Ordeberg, Gunvor, additional, Hjelmstedt, Anna, additional, and Lekander, Mats, additional
- Published
- 2011
- Full Text
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27. Does Low Molecular Weight Heparin Shorten Term Labor?
- Author
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Ekman-Ordeberg, G., primary, Akerud, A., additional, Dubicke, A., additional, Malmström, A., additional, and Hellgren, M., additional
- Published
- 2011
- Full Text
- View/download PDF
28. Depressive symptoms and inflammatory cytokines in women with preterm and term delivery
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Fransson, E., primary, Hjelmstedt, A., additional, Dubicke, A., additional, Byström, B., additional, Lekander, M., additional, and Ordeberg, G. Ekman, additional
- Published
- 2010
- Full Text
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29. Does Low Molecular Weight Heparin Shorten Term Labor?
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Ekman-Ordeberg, Gunvor, primary, Åkerud, Anna, additional, Dubicke, Aurelija, additional, Malmström, Anders, additional, and Hellgren, Margareta, additional
- Published
- 2010
- Full Text
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30. Depressive symptoms and inflammatory cytokines in women with preterm and term delivery
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G. Ekman Ordeberg, Aurelija Dubicke, Birgitta Byström, Anna Hjelmstedt, Mats Lekander, and Emma Fransson
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Behavioral Neuroscience ,Endocrine and Autonomic Systems ,business.industry ,Immunology ,Medicine ,Term delivery ,business ,Depressive symptoms ,Proinflammatory cytokine - Published
- 2010
31. Preterm Cervical Ripening in humans.
- Author
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Ekman-Ordeberg G and Dubicke A
- Abstract
Preterm birth (PTB) is the leading cause of neonatal mortality and morbidity. Despite the current treatment procedures, the incidence of PTB has not changed in the past thirty years. Incomplete understanding of the biological and patophysiological mechanisms underlying preterm delivery is the major obstacle to prevent PTB. Cervical ripening is necessary for vaginal delivery and understanding of preterm cervical ripening is required for developing new treatment strategies. Several important substances such as HMGB1 and its receptors, CRH and its receptors and numerous cytokines are localized in the cervix and undergo distinct changes in labour. Other important -molecules, such as CRH, CRH-BP, CRH-R1, CRH-R2, HMGB1, TLR2, TLR4, IL-10, IL-12, are localized in the cervical epithelium, also indicating their role in the process of cervical ripening during labour. Furthermore, CRH stimulates IL-8 secretion from both preterm and term cervical fibroblasts. Recent studies from our group show that major -inflammatory changes occur in the cervix at labour irrespective of gestational age. This indicates that cervical ripening at both term and preterm is an inflammatory process even if no infection is present. However, preterm cervical ripening still entails some differences from term cervical ripening, for example in the down-regulation of mRNA expression of Toll-like receptors (TLR-2 and TLR-4) and IL-12, higher levels of IL-10 in cervical epithelium, and presents different secretion patterns of cervical fibroblasts. Moreover, preterm cervical ripening, like preterm delivery itself, is a multifactorial disorder with pathways which are partly different from those involved in PPROM and infected preterm labour.
- Published
- 2012
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