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19 results on '"Duchez, David"'

3. A Multidisciplinary Modeling Approach of Plant Gas Exchange in Reduced Gravity Environments

Author

Poulet, Lucie, Vernay, Antoine, Duchez, David, Saudreau, Marc, Sharif, Helia, Kondyli, Vasiliki, Dussap, Claude-Gilles, Massa, Gioia D, and Wheeler, Raymond M

Subjects
Life Sciences (General)
Abstract

In-situ food production is a necessary step for human exploration of the solar system and requires a deep understanding of plant growth in reduced gravity environments. In particular, the lack of buoyancy-driven convection changes the gas exchange at the leaf surface, which decreases photosynthesis and transpiration rates, and ultimately biomass production. To understand the intricate relations between physical, chemical, and biochemical processes, the following methodology combines the development of a mechanistic model of plant growth in reduced gravity environments, computational fluid dynamics (CFD) simulations, and experiments in different time frames.The model presented here is a coupled mass and energy balance using the single round leaf assumption, including gravity as an entry parameter, and the leaf surface temperature as an output variable. Measures of the leaf surface temperature using infra-red cameras allow for a computation of the transpiration rate. This approach was followed to design a parabolic flight experiment, which performed 7 flights, and enabled data collection for model validation in different gravity and ventilation settings on a short time frame. Current measures of carbon assimilation and transpiration rate at the leaf and canopy level using an infra-red gas analyzer (Li-6800) in 1g lab conditions on several species will enable a validation on longer time frames and further calibration of the model. CFD studies both on the parabolic flight and on the lab experimental set-up allow the precise assessment of ventilation above the canopy and plants' leaves.Ultimately, this work will provide recommendations for the design of future plant growth hardware, especially on the lowest adequate ventilation for optimal plant growth in reduced gravity environments, as well as assessing biomass and oxygen production rates on planetary surfaces and space stations. This work was funded by CNES, CNRS, Clermont Auvergne Metropole, and NASA Space Biology through NASA postdoctoral program / USRA.

Published
2019

7. Valorization of co-products generated by Argan oil extraction process: Application to biodiesel production

Author

Zeghlouli, Jihane, Guendouz, A, Duchez, David, El Modafar, C, Michaud, Philippe, Delattre, Cédric, Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), and Tournayre, Christophe

Subjects
[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

11. Oxygen Regeneration by Algae Cultivation in Photo-Bioreactor for ISS Cabin Technology Demonstrator

Author

Chapuis, Dominique, Nebuloni, Stefano, Dainesi, Paolo, Laroche, Céline, Duchez, David, Dussap, Claude-Gilles, Volponi, Marco, Paille, Christel, RUAG Slip Rings SA, 1260 Nyon, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Directorate Of Technology, Engineering And Quality (TEC), Esa Estec, Keplerlaan 1, 2200ag Noordwijk, SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), and Tournayre, Christophe

Subjects
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
Abstract

International audience; Oxygen regeneration from ambient carbon dioxide is a fundamental technology building block for future life support systems for space applications. BIORAT1 Phase B2 project consists in the development of the Preliminary Design Review (PDR) level design of an On Board Demonstrator (OBD) to be hosted in European Drawer Rack 2(EDR2) facility on board of the ISS. The core of the OBD is a Photo-Bioreactor (PBR) filled with spirulina (Limnospira indica PCC 8005) producing oxygen from carbon dioxide and light by photosynthesis. A Liquid Loop (LL) transports the oxygen & carbon dioxide dissolved into the cultivation medium liquid between the Photobioreactor (PBR) and the ISS cabin ambient air. The Gas Exchange Module (GEM) enables the exchange of Oxygen & Carbon Dioxyde separates the cultivation medium liquid to the ambient air while keeping the liquid inside the LL. The design of this flight hardware is supported by tests results obtained with a Bread Board Model (BBM). In this paper, we present the results of the long duration spirulina cultivation test performed with the BBM. allowing verification of the long term functionality of the PBR & LL including the GEM. The PBR performances together with correlation to the model of the cultivated algae growth and oxygen production are presented. Future development and expected results and perspectives are also presented and discussed.

Published
2020

12. BIORAT: Oxygen Recycling between an Algae Photo-bioreactor and a Consumer

Author

Chapuis, D., Nebuloni, S., Dainesi, P., Gass, S., Laroche, Céline, Duchez, David, Dussap, Claude-Gilles, Demey, D., Delahaye, A, Gerbi, O., Paille, Christel, RUAG Space GmbH, Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), QinetiQ, Sherpa Engineering, Agence Spatiale Européenne (ESA), European Space Agency (ESA), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Agence Spatiale Européenne = European Space Agency (ESA), and Tournayre, Christophe

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, ComputingMilieux_MISCELLANEOUS, [SDV.BIO] Life Sciences [q-bio]/Biotechnology
Abstract

International audience

Published
2018

13. Lactobacillus reuteri suppresses enterohaemorrhagic Escherichia coli O157:H7 in bovine ruminal fluid

Author

Bertin, Yolande, Habouzit, Chloé, Duniere, Lysiane, Laurier, Marie, Durand, Alexandra, Duchez, David, Segura, Audrey, Thevenot-Sergentet, Delphine, Baruzzi, Federico, Durand, Frédérique, Forano, Evelyne, Microbiologie Environnement Digestif Santé (MEDIS), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Lallemand S.A.S., Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Institute of Sciences of Food Production (ISPA), National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), ProdInra, Migration, INRA Clermont-Ferrand-Theix-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), SIGMA Clermont (SIGMA Clermont)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), and Consiglio Nazionale delle Ricerche (CNR)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, food and beverages, bacteria, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Abstract

International audience; The bovine gastrointestinal tract (GIT) is the main reservoir for enterohaemorrhagic Escherichia coli (EHEC) responsible for food-borne infections. Therefore, it is crucial to develop strategies, such as EHEC suppression by antagonistic microorganisms, to reduce EHEC survival in the GIT of cattle and to limit shedding and food contamination. Most human-derived Lactobacillus reuteri strains produce hydroxypropionaldehyde (HPA), an antimicrobial compound, during anaerobic reduction of glycerol. The capacity of L. reuteri LB1-7 to produce HPA and its antimicrobial activity against EHEC FCH6 were evaluated in bovine rumen fluid (RF) under strict anaerobiosis. EHEC was totally suppressed when incubated in RF inoculated with L. reuteri LB1-7 and supplemented with 80 mM glycerol. The addition of LB1-7 or glycerol alone did not modify EHEC survival in RF. Glycerol was converted to HPA (up to 14 mM) and lactate (30 mM) by LB1-7, but only HPA production seemed to be responsible for EHEC suppression. Furthermore, L. reuteri LB1-7 did not have major adverse effects on ruminal digestion of common feedstuffs. The bactericidal activity of L. reuteri LB1-7, the concentration of glycerol required and the level of HPA secreted depended on physiological and ecological environments. In vitro experiments also showed that EHEC inoculated in rumen fluid and exposed to L. reuteri and glycerol had a very limited growth in rectal contents.

Published
2017

14. Lactobacillus reuteri suppresses E. coli O157:H7 in bovine ruminal fluid: Toward a pre-slaughter strategy to improve food safety?

Author

Bertin, Yolande, primary, Habouzit, Chloé, additional, Dunière, Lysiane, additional, Laurier, Marie, additional, Durand, Alexandra, additional, Duchez, David, additional, Segura, Audrey, additional, Thévenot-Sergentet, Delphine, additional, Baruzzi, Federico, additional, Chaucheyras-Durand, Frédérique, additional, and Forano, Evelyne, additional

Published
2017
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15. Caractérisation d'un bioréacteur pour la culture de Saccharomyces cerevisiae soumis à des champs électromagnétiques en environnements anéchoïqu et réverbérant

Author

Bertrand, Emmanuel, Pasquier, Christophe, Girard, Sébastien, Duchez, David, Pons, Agnès, Creuly, Catherine, Dussap, Claude-Gilles, Génie des Procédés, Energétique et Biosystèmes (GePEB), Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS)-SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Photonique, Ondes, Nanomatériaux (PHOTON), and Bertrand, Emmanuel

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS, [SDV.BIO] Life Sciences [q-bio]/Biotechnology
Abstract

International audience

Published
2014

16. Caractérisation d'un bioréacteur pour la culture de Saccharomyces cerevisiae soumis à des champs électro-magnétiques en environnements anéchoïque et réverbérant

Author

Bertrand, Emmanuel, Pasquier, Christophe, Girard, Sébastien, Duchez, David, Pons, Agnès, Creuly, Catherine, Dussap, Claude-Gilles, Biodiversité et Biotechnologie Fongiques (BBF), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-École Centrale de Marseille (ECM), Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Institut Pascal (IP), SIGMA Clermont (SIGMA Clermont)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), and ProdInra, Migration

Subjects
[SPI.OTHER]Engineering Sciences [physics]/Other, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, modélisation numérique, [SPI.OTHER] Engineering Sciences [physics]/Other, champ électromagnétique, propriétés diélectriques, chambre réverbérante à brassage de mode, Saccharomyces cerevisiae, chambre anéchoïque, [SDV.BIO] Life Sciences [q-bio]/Biotechnology
Abstract

National audience; Ce travail caractérise un dispositif expérimental conçu pour étudier les effets des ondes électro magnétiques sur des cellules eucaryotes (Saccharomyces cerevisiae) cultivées en bioréacteur tout en permettant les régulations de température, de pH, de pO2, d’agitation et d’aération influençant la croissance des cellules. Les simulations numériques réalisées montrent que l’atténuation des ondes électromagnétiques au sein du milieu de culture est importante et que les modifications des propriétés diélectriques du milieu liées à la fréquence des ondes incidentes et aux variations de composition du milieu au cours de la culture peuvent avoir une influence sur le niveau d’exposition des cellules aux champs électromagnétiques. Les essais réalisés en environnements anéchoïque et réverbérant ne mettent pas en évidence d’effet sur le taux de croissance et les rendements de production des différents métabolites de la levure soumise aux champs électromagnétiques.

Published
2014

19. Enhanced growth and metabolite production from a novel strain of Porphyridium sp.

Author

Tounsi L, Ben Hlima H, Derbel H, Duchez D, Gardarin C, Dubessay P, Drira M, Fendri I, Michaud P, and Abdelkafi S

Subjects
Chlorophyll A metabolism, Starch, Photosynthesis, Biomass, Porphyridium genetics, Porphyridium metabolism, Microalgae metabolism
Abstract

Microalgae are capable of generating numerous metabolites that possess notable biological activities and hold substantial promise for various industrial applications. Nevertheless, the taxonomic diversity of these photosynthetic microorganisms has not received thorough investigation. Using the 18S rRNA encoding gene, a recently discovered strain originating from the Tunisian coast (the governorate of Mahdia) was identified as a member of the Porphyridium genus. The growth response as well as the metabolite accumulation of Porphyridium sp. to different culture media (Pm, F/2, and Hemerick) was investigated over a period of 52 days. The highest biomass production was recorded with Pm medium (2 × 10 7 cell/mL). The apparent growth rates (µ) and the doubling time (D t ) were about 0.081 day -1 and 12.34 days, respectively. The highest chlorophyll a (0.678 ± 0.005 pg/cell), total carotenoids (0.18 ± 0.003 pg/cell), phycoerythrin (3.88 ± 0.003 pg/cell), and proteins (14.58 ± 0.35 pg/cell) contents were observed with F/2 medium. Cultivating Porphyridium sp. in both F/2 and Hemerick media yielded similar levels of starch accumulation. The Hemerick medium has proven to be the most suitable for the production of lipids (2.23% DW) and exopolysaccharides (5.41 ± 0.56 pg/cell).

Published
2024
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