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155 results on '"Duignan, Pádraig J."'

2. Respiratory Tract Explant Infection Dynamics of Influenza A Virus in California Sea Lions, Northern Elephant Seals, and Rhesus Macaques

Author

Liu, Hongwei, Plancarte, Magdalena, Ball, Erin E, Weiss, Christopher M, Gonzales-Viera, Omar, Holcomb, Karen, Ma, Zhong-Min, Allen, A Mark, Reader, J Rachel, Duignan, Pádraig J, Halaska, Barbie, Khan, Zenab, Kriti, Divya, Dutta, Jayeeta, van Bakel, Harm, Jackson, Kenneth, Pesavento, Patricia A, Boyce, Walter M, and Coffey, Lark L

Subjects
Microbiology, Medical Microbiology, Biomedical and Clinical Sciences, Biological Sciences, Lung, Biodefense, Influenza, Pneumonia & Influenza, Emerging Infectious Diseases, Infectious Diseases, 2.2 Factors relating to the physical environment, Infection, Animals, Dogs, Host Specificity, Influenza A virus, Kinetics, Macaca mulatta, Madin Darby Canine Kidney Cells, Models, Biological, Orthomyxoviridae Infections, Respiratory System, Respiratory Tract Infections, Sea Lions, Seals, Earless, Species Specificity, Viral Load, Viral Tropism, explant, infection dynamics, influenza A virus, kinetics, marine mammal, respiratory viruses, rhesus macaque, tropism, Agricultural and Veterinary Sciences, Medical and Health Sciences, Virology, Agricultural, veterinary and food sciences, Biological sciences, Biomedical and clinical sciences
Abstract

To understand susceptibility of wild California sea lions and Northern elephant seals to influenza A virus (IAV), we developed an ex vivo respiratory explant model and used it to compare infection kinetics for multiple IAV subtypes. We first established the approach using explants from colonized rhesus macaques, a model for human IAV. Trachea, bronchi, and lungs from 11 California sea lions, 2 Northern elephant seals, and 10 rhesus macaques were inoculated within 24 h postmortem with 6 strains representing 4 IAV subtypes. Explants from the 3 species showed similar IAV infection kinetics, with peak viral titers 48 to 72 h post-inoculation that increased by 2 to 4 log10 PFU/explant relative to the inoculum. Immunohistochemistry localized IAV infection to apical epithelial cells. These results demonstrate that respiratory tissue explants from wild marine mammals support IAV infection. In the absence of the ability to perform experimental infections of marine mammals, this ex vivo culture of respiratory tissues mirrors the in vivo environment and serves as a tool to study IAV susceptibility, host range, and tissue tropism. IMPORTANCE Although influenza A virus can infect marine mammals, a dearth of marine mammal cell lines and ethical and logistical challenges prohibiting experimental infections of living marine mammals mean that little is known about IAV infection kinetics in these species. We circumvented these limitations by adapting a respiratory tract explant model first to establish the approach with rhesus macaques and then for use with explants from wild marine mammals euthanized for nonrespiratory medical conditions. We observed that multiple strains representing 4 IAV subtypes infected trachea, bronchi, and lungs of macaques and marine mammals with variable peak titers and kinetics. This ex vivo model can define infection dynamics for IAV in marine mammals. Further, use of explants from animals euthanized for other reasons reduces use of animals in research.

Published
2021

5. The Symptomatology and Diagnosis of Domoic Acid Toxicosis in Stranded California Sea Lions: A Review and Evaluation of 20 Years of Cases to Guide Prognosis

Author

McClain, Abby M., primary, Field, Cara L., additional, Norris, Tenaya A., additional, Borremans, Benny, additional, Duignan, Pádraig J., additional, Johnson, Shawn P., additional, Whoriskey, Sophie T., additional, Thompson-Barbosa, Lorraine, additional, and Gulland, Frances M. D., additional

Published
2023
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8. Human influenza A virus H1N1 in marine mammals in California, 2019

Author

Plancarte, Magdalena, primary, Kovalenko, Ganna, additional, Baldassano, Julie, additional, Ramírez, Ana L., additional, Carrillo, Selina, additional, Duignan, Pádraig J., additional, Goodfellow, Ian, additional, Bortz, Eric, additional, Dutta, Jayeeta, additional, van Bakel, Harm, additional, and Coffey, Lark L., additional

Published
2023
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11. Human influenza A virus H1N1 in marine mammals in California, 2019

Author

Plancarte, Magdalena, Kovalenko, Ganna, Baldassano, Julie, Ramírez, Ana L, Carrillo, Selina, Duignan, Pádraig J, Goodfellow, Ian, Bortz, Eric, Dutta, Jayeeta, Van Bakel, Harm, Coffey, Lark L, Coffey, Lark L [0000-0002-0718-5146], and Apollo - University of Cambridge Repository

Subjects
Influenza A Virus, H1N1 Subtype, Influenza A virus, Seals, Earless, Influenza, Human, Animals, Humans, California, Caniformia
Abstract

Acknowledgements: We acknowledge Jesierose Poblacion, Carlos Rios, Christine Fontaine, Barbie Halaska, and the veterinary staff at TMMC, who conducted marine mammal sampling and provided swabs and serum under NOAA permit 18786–04. We thank Wendy Puryear, Nichola Hill, and Jonathan Runstadler at Tufts University, who provided HI protocols, and Randy Albrecht at the Icahn School of Medicine at Mount Sinai, who provided control sera for HI assays. Hon Ip at the National Wildlife Health Center provided the IAV H3N8 isolate. We are grateful to all authors from the originating laboratories responsible for obtaining the human specimens, as well as the submitting laboratories where the genome data were generated and shared via GISAID., Funder: UC Davis School of Veterinary Medicine Endowment Fund, From 2011-2018, we conducted surveillance in marine mammals along the California coast for influenza A virus (IAV), frequently detecting anti-influenza antibodies and intermittently detecting IAV. In spring 2019, this pattern changed. Despite no change in surveillance intensity, we detected IAV RNA in 10 samples in March and April, mostly in nasal and rectal swabs from northern elephant seals (Mirounga angustirostris). Although virus isolation was unsuccessful, IAV sequenced from one northern elephant seal nasal swab showed close genetic identity with pandemic H1N1 IAV subclade 6B.1A.1 that was concurrently circulating in humans in the 2018/19 influenza season. This represents the first report of human A(H1N1)pdm09 IAV in northern elephant seals since 2010, suggesting IAV continues to spill over from humans to pinnipeds.

Published
2023

12. Human influenza A virus H1N1 in marine mammals in California, 2019

Author

Plancarte, Magdalena, primary, Kovalenko, Ganna, additional, Baldassano, Julie, additional, Ramírez, Ana L., additional, Carrillo, Selina, additional, Duignan, Pádraig J., additional, Goodfellow, Ian, additional, Bortz, Eric, additional, Dutta, Jayeeta, additional, van Bakel, Harm, additional, and Coffey, Lark L., additional

Published
2022
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15. Small Blubber Samples (50 mg) Sufficient for Analyses of 10 Stress and Reproductive Steroid Hormones in Gray and Fin Whales via Liquid Chromatography Mass Spectrometry

Author

Wittmaack, Christiana, primary, Urbán Ramírez, Jorge, additional, Bernot-Simon, Daniela, additional, Martínez-Aguilar, Sergio, additional, Subbiah, Seenivasan, additional, Surles, James G., additional, Looney, Mary, additional, Kumar, Naveen, additional, Halaska, Barbie, additional, Duignan, Pádraig J., additional, Knauss, Madelyn, additional, Burns, Kristen, additional, and Godard-Codding, Céline A. J., additional

Published
2022
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22. Clinical Signs and Pathology Associated With Domoic Acid Toxicosis in Southern Sea Otters (Enhydra lutris nereis)

Author

Miller, Melissa A., primary, Moriarty, Megan E., additional, Duignan, Pádraig J., additional, Zabka, Tanja S., additional, Dodd, Erin, additional, Batac, Francesca I., additional, Young, Colleen, additional, Reed, Angelina, additional, Harris, Michael D., additional, Greenwald, Katherine, additional, Kudela, Raphael M., additional, Murray, Michael J., additional, Gulland, Frances M. D., additional, Miller, Peter E., additional, Hayashi, Kendra, additional, Gunther-Harrington, Catherine T., additional, Tinker, Martin T., additional, and Toy-Choutka, Sharon, additional

Published
2021
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24. Demodectic mange in threatened southern sea otters (Enhydralutrisnereis)

Author

Javeed, Nadia, primary, Foley, Janet, additional, Oliver‐Guimera, Arturo, additional, Affolter, Verena K., additional, Keel, Michael K., additional, Reed, Angelina, additional, Pesapane, Risa, additional, Duignan, Pádraig J., additional, Murray, Michael, additional, Tinker, MT, additional, and Miller, Melissa A., additional

Published
2021
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25. Persistent Contaminants and Herpesvirus OtHV1 Are Positively Associated With Cancer in Wild California Sea Lions (Zalophus californianus)

Author

Gulland, Frances M. D., primary, Hall, Ailsa J., additional, Ylitalo, Gina M., additional, Colegrove, Kathleen M., additional, Norris, Tenaya, additional, Duignan, Pádraig J., additional, Halaska, Barbie, additional, Acevedo Whitehouse, Karina, additional, Lowenstine, Linda J., additional, Deming, Alissa C., additional, and Rowles, Teresa K., additional

Published
2020
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27. Respiratory tract explant infection dynamics of influenza A virus in California sea lions, northern elephant seals, and rhesus macaques

Author

Liu, Hongwei, primary, Plancarte, Magdalena, additional, Ball, Erin. E., additional, Weiss, Christopher M., additional, Gonzales-Viera, Omar, additional, Holcomb, Karen, additional, Ma, Zhong-Min, additional, Allen, A. Mark, additional, Reader, J. Rachel, additional, Duignan, Pádraig J., additional, Halaska, Barbie, additional, Khan, Zenab, additional, Kriti, Divya, additional, Dutta, Jayeeta, additional, van Bakel, Harm, additional, Jackson, Kenneth, additional, Pesavento, Patricia A., additional, Boyce, Walter M., additional, and Coffey, Lark L., additional

Published
2020
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29. Emergence of a Zoonotic Pathogen in a Coastal Marine Sentinel: Capillaria hepatica (syn. Calodium hepaticum)-Associated Hepatitis in Southern Sea Otters (Enhydra lutris nereis)

Author

Miller, Melissa A., primary, Duignan, Pádraig J., additional, Dodd, Erin, additional, Batac, Francesca, additional, Staedler, Michelle, additional, Tomoleoni, Joseph A., additional, Murray, Michael, additional, Harris, Heather, additional, and Gardiner, Chris, additional

Published
2020
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32. An insight into the epidemiology of dolphin morbillivirus worldwide

Author

Van Bressem, Marie-Françoise, Waerebeek, Koen Van, Jepson, Paul D, Raga, Juan Antonio, Duignan, Padraig J, Nielsen, Ole, Di Beneditto, Ana Paula, Siciliano, Salvatore, Ramos, Renata, Kant, Wendy, Peddemors, Vic, Kinoshita, Reimi, Ross, Peter S, López-Fernandez, Alfredo, Evans, Karen, Crespo, Enrique, and Barrett, Tom

Published
2001
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49. Cetacean Morbillivirus: Current Knowledge and Future Directions.

Author

Van Bressem, Marie-Françoise, Duignan, Pádraig J., Banyard, Ashley, Barbieri, Michelle, Colegrove, Kathleen M, De Guise, Sylvain, Di Guardo, Giovanni, Dobson, Andrew, Domingo, Mariano, Fauquier, Deborah, Fernandez, Antonio, Goldstein, Tracey, Grenfell, Bryan, Groch, Kátia R., Gulland, Frances, Jensen, Brenda A., Jepson, Paul D., Hall, Ailsa, Kuiken, Thijs, and Mazzariol, Sandro

Subjects
*MORBILLIVIRUSES, *CETACEA, *TOOTHED whales, *DELPHINIDAE, *IMMUNOHISTOCHEMISTRY, *DISEASES
Abstract

We review the molecular and epidemiological characteristics of cetacean morbillivirus (CeMV) and the diagnosis and pathogenesis of associated disease, with six different strains detected in cetaceans worldwide. CeMV has caused epidemics with high mortality in odontocetes in Europe, the USA and Australia. It represents a distinct species within the Morbillivirus genus. Although most CeMV strains are phylogenetically closely related, recent data indicate that morbilliviruses recovered from Indo-Pacific bottlenose dolphins (Tursiops aduncus), from Western Australia, and a Guiana dolphin (Sotalia guianensis), from Brazil, are divergent. The signaling lymphocyte activation molecule (SLAM) cell receptor for CeMV has been characterized in cetaceans. It shares higher amino acid identity with the ruminant SLAM than with the receptors of carnivores or humans, reflecting the evolutionary history of these mammalian taxa. In Delphinidae, three amino acid substitutions may result in a higher affinity for the virus. Infection is diagnosed by histology, immunohistochemistry, virus isolation, RT-PCR, and serology. Classical CeMV-associated lesions include bronchointerstitial pneumonia, encephalitis, syncytia, and lymphoid depletion associated with immunosuppression. Cetaceans that survive the acute disease may develop fatal secondary infections and chronic encephalitis. Endemically infected, gregarious odontocetes probably serve as reservoirs and vectors. Transmission likely occurs through the inhalation of aerosolized virus but mother to fetus transmission was also reported. [ABSTRACT FROM AUTHOR]

Published
2014
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50. Phocine Distemper Virus: Current Knowledge and Future Directions.

Author

Duignan, Pádraig J., Van Bressem, Marie-Françoise, Baker, Jason D., Barbieri, Michelle, Colegrove, Kathleen M., De Guise, Sylvain, de Swart, Rik L., Di Guardo, Giovanni, Dobson, Andrew, Paul Duprex, W., Early, Greg, Fauquier, Deborah, Goldstein, Tracey, Goodman, Simon J., Grenfell, Bryan, Groch, Kátia R., Gulland, Frances, Hall, Ailsa, Jensen, Brenda A., and Lamy, Karina

Subjects
*DISTEMPER, *SEALS (Animals), *EPIDEMIOLOGY, *PINNIPEDIA, *IMMUNE response, *DISEASES
Abstract

Phocine distemper virus (PDV) was first recognized in 1988 following a massive epidemic in harbor and grey seals in north-western Europe. Since then, the epidemiology of infection in North Atlantic and Arctic pinnipeds has been investigated. In the western North Atlantic endemic infection in harp and grey seals predates the European epidemic, with relatively small, localized mortality events occurring primarily in harbor seals. By contrast, PDV seems not to have become established in European harbor seals following the 1988 epidemic and a second event of similar magnitude and extent occurred in 2002. PDV is a distinct species within the Morbillivirus genus with minor sequence variation between outbreaks over time. There is now mounting evidence of PDV-like viruses in the North Pacific/Western Arctic with serological and molecular evidence of infection in pinnipeds and sea otters. However, despite the absence of associated mortality in the region, there is concern that the virus may infect the large Pacific harbor seal and northern elephant seal populations or the endangered Hawaiian monk seals. Here, we review the current state of knowledge on PDV with particular focus on developments in diagnostics, pathogenesis, immune response, vaccine development, phylogenetics and modeling over the past 20 years. [ABSTRACT FROM AUTHOR]

Published
2014
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