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Methanogenic archaea are chemolithotrophic prokaryotes that can reduce carbon dioxide with hydrogen gas to form methane. These microorganisms make a significant contribution to the global carbon cycle, with methanogenic archaea from anoxic environments estimated to contribute > 500 million tons of global methane annually. Archaeal methanogenesis is dependent on the methanofurans; aminomethylfuran containing coenzymes that act as the primary C 1 acceptor molecule during carbon dioxide fixation. Although the biosynthetic pathway to the methanofurans has been elucidated, structural adaptations which confer thermotolerance to Mfn enzymes from extremophilic archaea are yet to be investigated. Here we focus on the methanofuran biosynthetic enzyme MfnB, which catalyses the condensation of two molecules of glyceralde-3-phosphate to form 4‑(hydroxymethyl)-2-furancarboxaldehyde-phosphate. In this study, MfnB enzymes from the hyperthermophile Methanocaldococcus jannaschii and the mesophile Methanococcus maripaludis have been recombinantly overexpressed and purified to homogeneity. Thermal unfolding studies, together with steady-state kinetic assays, demonstrate thermoadaptation in the M. jannaschii enzyme. Molecular dynamics simulations have been used to provide a structural explanation for the observed properties. These reveal a greater number of side chain interactions in the M. jannaschii enzyme, which may confer protection from heating effects by enforcing spatial residue constraints., (© 2024. The Author(s).)

A new test technique and bespoke apparatus to conduct high strain rate measurements of the tensile response of materials are presented. The new test method is applicable to brittle solids and composites as well as high-performance fibres, yarns and tapes used in composite construction. In this study, the dynamic response of monolithic poly(methyl methacrylate) and unidirectional composites based on Dyneema® tape, Dyneema® SK75 yarn and Kevlar® 49 yarn are explored. The technique allows early force equilibrium and yields valid tensile stress-strain curves, which include part of the elastic material response. The new method also enables investigation of size effects in tape and yarn materials, allowing testing of specimens of arbitrary length., Competing Interests: We declare we have no competing interests., (© 2019 The Author(s).)

In this study, an experimental and numerical investigation is presented on the effect of thickness and test rate within the pseudo static regime on the tensile properties of Dyneema ® HB26 laminates. A detailed experimental presentation on the tensile testing of different thickness is presented and highlights the commonly seen observation that the tensile strength of a laminate reduces as a function of the specimen thickness. To understand these experimental observations, a constitutive material model of the individual macro fibril is developed and applied to modelling the fibre and upscaling to the laminate. The modelling strategy is implemented into ls-dyna and used to perform a parameter study on the specimen geometries used in the experimental study. The model assumes that the fibril strength is a function of the amorphous volume within the fibre and hence fibril. It can be observed that the experimental behaviour can be simulated by modelling the interface between laminate plies and the fibril, and hence fibre failure. The weak interfaces from the fibril to the laminate scale make the testing of fibres and laminates very difficult. Hence, it is proposed that the intrinsic fibril strength should be used as a measure of strength, and the fundamental strength is determined through numerical studies.