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3. Spatiotemporal localization of proteins in mycobacteria

Author

Zhu, Junhao, Wolf, Ian D., Dulberger, Charles L., Won, Harim I., Kester, Jemila C., Judd, Julius A., Wirth, Samantha E., Clark, Ryan R., Li, Yawei, Luo, Yuan, Gray, Todd A., Wade, Joseph T., Derbyshire, Keith M., Fortune, Sarah M., and Rubin, Eric J.

Published
2021
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5. A multivalent mRNA monkeypox virus vaccine (BNT166) protects mice and macaques from orthopoxvirus disease

Author

Zuiani, Adam, primary, Dulberger, Charles L., additional, De Silva, Nilushi S., additional, Marquette, Meghan, additional, Lu, Yu-Jung, additional, Palowitch, Gavin M., additional, Dokic, Anja, additional, Sanchez-Velazquez, Ricardo, additional, Schlatterer, Katja, additional, Sarkar, Sanjay, additional, Kar, Swagata, additional, Chawla, Bhavna, additional, Galeev, Alibek, additional, Lindemann, Claudia, additional, Rothenberg, Daniel A., additional, Diao, Huitian, additional, Walls, Alexandra C., additional, Addona, Theresa A., additional, Mensa, Federico, additional, Vogel, Annette B., additional, Stuart, Lynda M., additional, van der Most, Robbert, additional, Srouji, John R., additional, Türeci, Özlem, additional, Gaynor, Richard B., additional, Şahin, Uğur, additional, and Poran, Asaf, additional

Published
2024
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9. Spec-seq unveils transcriptional subpopulations of antibody-secreting cells following influenza vaccination

Author

Neu, Karlynn E., Guthmiller, Jenna J., Huang, Min, La, Jennifer, Vieira, Marcos C., Kim, Kangchon, Zheng, Nai-Ying, Cortese, Mario, Tepora, Micah E., Hamel, Natalie J., Rojas, Karla Thatcher, Henry, Carole, Shaw, Dustin, Dulberger, Charles L., Pulendran, Bali, Cobey, Sarah, Khan, Aly A., and Wilson, Patrick C.

Subjects
Usage, Prevention, Research, Influenza vaccines -- Usage, Influenza -- Prevention, Blood plasma -- Research
Abstract

IntroductionThe medical field has relied on vaccines for improving human health since the days of smallpox inoculation--long before antibodies were identified (1, 2). Vaccines induce a humoral immune response, in [...], Vaccines are among the most effective public health tools for combating certain infectious diseases such as influenza. The role of the humoral immune system in vaccine-induced protection is widely appreciated; however, our understanding of how antibody specificities relate to B cell function remains limited due to the complexity of polyclonal antibody responses. To address this, we developed the Spec-seq framework, which allows for simultaneous monoclonal antibody (mAb) characterization and transcriptional profiling from the same single cell. Here, we present the first application of the Spec-seq framework, which we applied to human plasmablasts after influenza vaccination in order to characterize transcriptional differences governed by B cell receptor (BCR) isotype and vaccine reactivity. Our analysis did not find evidence of long-term transcriptional specialization between plasmablasts of different isotypes. However, we did find enhanced transcriptional similarity between clonally related B cells, as well as distinct transcriptional signatures ascribed by BCR vaccine recognition. These data suggest IgG and IgA vaccine-positive plasmablasts are largely similar, whereas IgA vaccine-negative cells appear to be transcriptionally distinct from conventional, terminally differentiated, antigen-induced peripheral blood plasmablasts.

Published
2019
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10. Tuberculosis treatment failure associated with evolution of antibiotic resilience

Author

Liu, Qingyun, primary, Zhu, Junhao, additional, Dulberger, Charles L., additional, Stanley, Sydney, additional, Wilson, Sean, additional, Chung, Eun Seon, additional, Wang, Xin, additional, Culviner, Peter, additional, Liu, Yue J., additional, Hicks, Nathan D., additional, Babunovic, Gregory H., additional, Giffen, Samantha R., additional, Aldridge, Bree B., additional, Garner, Ethan C., additional, Rubin, Eric J., additional, Chao, Michael C., additional, and Fortune, Sarah M., additional

Published
2022
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16. Antibiotic action revealed by real-time imaging of the mycobacterial membrane

Author

Wuo, Michael G., primary, Dulberger, Charles L., additional, Brown, Robert A., additional, Sturm, Alexander, additional, Ultee, Eveline, additional, Bloom-Ackermann, Zohar, additional, Choi, Catherine, additional, Garner, Ethan C., additional, Briegel, Ariane, additional, Hung, Deborah T., additional, Rubin, Eric J., additional, and Kiessling, Laura L., additional

Published
2022
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21. The Conserved Translation Factor LepA Is Required for Optimal Synthesis of a Porin Family in Mycobacterium smegmatis

Author

Fishbein, Skye R. S., primary, Tomasi, Francesca G., additional, Wolf, Ian D., additional, Dulberger, Charles L., additional, Wang, Albert, additional, Keshishian, Hasmik, additional, Wallace, Luke, additional, Carr, Steven A., additional, Ioerger, Thomas R., additional, Rego, E. Hesper, additional, and Rubin, Eric J., additional

Published
2021
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23. Prophage-encoded phage defense proteins with cognate self-immunity

Author

Owen, Siân V., primary, Wenner, Nicolas, additional, Dulberger, Charles L., additional, Rodwell, Ella V., additional, Bowers-Barnard, Arthur, additional, Quinones-Olvera, Natalia, additional, Rigden, Daniel J., additional, Rubin, Eric J., additional, Garner, Ethan C., additional, Baym, Michael, additional, and Hinton, Jay C. D., additional

Published
2020
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26. Spec-seq unveils transcriptional subpopulations of antibody-secreting cells following influenza vaccination

Author

Neu, Karlynn E., primary, Guthmiller, Jenna J., additional, Huang, Min, additional, La, Jennifer, additional, Vieira, Marcos C., additional, Kim, Kangchon, additional, Zheng, Nai-Ying, additional, Cortese, Mario, additional, Tepora, Micah E., additional, Hamel, Natalie J., additional, Rojas, Karla Thatcher, additional, Henry, Carole, additional, Shaw, Dustin, additional, Dulberger, Charles L., additional, Pulendran, Bali, additional, Cobey, Sarah, additional, Khan, Aly A., additional, and Wilson, Patrick C., additional

Published
2018
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28. MAIT recognition of a stimulatory bacterial antigen bound to MR11

Author

López-Sagaseta, Jacinto, Dulberger, Charles L., McFedries, Amanda, Cushman, Mark, Saghatelian, Alan, and Adams, Erin J.

Subjects
Salmonella typhimurium, Antigen Presentation, Antigens, Bacterial, Receptors, Antigen, T-Cell, alpha-beta, Histocompatibility Antigens Class I, Crystallography, X-Ray, Lymphocyte Activation, Article, Protein Structure, Tertiary, Minor Histocompatibility Antigens, T-Lymphocyte Subsets, Multiprotein Complexes, Escherichia coli, Humans, Protein Binding
Abstract

MR1-restricted mucosal-associated invariant T (MAIT) cells represent a subpopulation of αβ T cells with innate-like properties and limited TCR diversity. MAIT cells are of interest because of their reactivity against bacterial and yeast species, suggesting that they play a role in defense against pathogenic microbes. Despite the advances in understanding MAIT cell biology, the molecular and structural basis behind their ability to detect MR1-Ag complexes is unclear. In this study, we present our structural and biochemical characterization of MAIT TCR engagement of MR1 presenting an Escherichia coli-derived stimulatory ligand, rRL-6-CH2OH, previously found in Salmonella typhimurium. We show a clear enhancement of MAIT TCR binding to MR1 due to the presentation of this ligand. Our structure of a MAIT TCR/MR1/rRL-6-CH2OH complex shows an evolutionarily conserved binding orientation, with a clear role for both the CDR3α and CDR3β loops in recognizing the rRL-6-CH2OH stimulatory ligand. We also present two additional xenoreactive MAIT TCR/MR1 complexes that recapitulate the docking orientation documented previously, despite having variation in the CDR2β and CDR3β loop sequences. Our data support a model by which MAIT TCRs engage MR1 in a conserved fashion, with their binding affinities modulated by the nature of the MR1-presented Ag or diversity introduced by alternate Vβ usage or CDR3β sequences.

Published
2013

30. The Deinococcus radiodurans DR1245 Protein, a DdrB Partner Homologous to YbjN Proteins and Reminiscent of Type III Secretion System Chaperones

Author

Norais, Cédric, primary, Servant, Pascale, additional, Bouthier-de-la-Tour, Claire, additional, Coureux, Pierre-Damien, additional, Ithurbide, Solenne, additional, Vannier, Françoise, additional, Guerin, Philippe P., additional, Dulberger, Charles L., additional, Satyshur, Kenneth A., additional, Keck, James L., additional, Armengaud, Jean, additional, Cox, Michael M., additional, and Sommer, Suzanne, additional

Published
2013
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31. The Deinococcus radiodurans DR1245 Protein, a DdrB Partner Homologous to YbjN Proteins and Reminiscent of Type III Secretion System Chaperones.

Author

Norais, Cédric, Servant, Pascale, Bouthier-de-la-Tour, Claire, Coureux, Pierre-Damien, Ithurbide, Solenne, Vannier, Françoise, Guerin, Philippe P., Dulberger, Charles L., Satyshur, Kenneth A., Keck, James L., Armengaud, Jean, Cox, Michael M., and Sommer, Suzanne

Subjects
DEINOCOCCUS radiodurans, PROTEINS, MOLECULAR chaperones, IONIZING radiation, DNA repair, PROTEIN structure, MICROBIAL physiology, MOLECULAR biology
Abstract

The bacterium Deinococcus radiodurans exhibits an extreme resistance to ionizing radiation. A small subset of Deinococcus genus-specific genes were shown to be up-regulated upon exposure to ionizing radiation and to play a role in genome reconstitution. These genes include an SSB-like protein called DdrB. Here, we identified a novel protein encoded by the dr1245 gene as an interacting partner of DdrB. A strain devoid of the DR1245 protein is impaired in growth, exhibiting a generation time approximately threefold that of the wild type strain while radioresistance is not affected. We determined the three-dimensional structure of DR1245, revealing a relationship with type III secretion system chaperones and YbjN family proteins. Thus, DR1245 may display some chaperone activity towards DdrB and possibly other substrates. [ABSTRACT FROM AUTHOR]

Published
2013
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32. Molecular characterization of the archaic HLA-B*73:01 allele reveals presentation of a unique peptidome and skewed engagement by KIR2DL2.

Author

Ross P, Hilton HG, Lodwick J, Slezak T, Guethlein LA, McMurtrey CP, Han AS, Nielsen M, Yong D, Dulberger CL, Nolan KT, Roy S, Castro CD, Hildebrand WH, Zhao M, Kossiakoff A, Parham P, and Adams EJ

Abstract

HLA class I alleles of archaic origin may have been retained in modern humans because they provide immunity against diseases to which archaic humans had evolved resistance. According to this model, archaic introgressed alleles were somehow distinct from those that evolved in African populations. Here we show that HLA-B*73:01, a rare allotype with putative archaic origins, has a relatively rare peptide binding motif with an unusually long-tailed peptide length distribution. We also find that HLA-B*73:01 combines a restricted and unique peptidome with high-cell surface expression, characteristics that make it well-suited to combat one or a number of closely-related pathogens. Furthermore, a crystal structure of HLA-B*73:01 in complex with KIR2DL2 highlights differences from previously solved structures with HLA-C molecules. These molecular characteristics distinguish HLA-B*73:01 from other HLA class I alleles previously investigated and may have provided early modern human migrants that inherited this allele with a selective advantage as they colonized Europe and Asia.

Published
2024
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