1. Placental Passage of Protopine in an Ex Vivo Human Perfusion System
- Author
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Spiess, Deborah, Abegg, Vanessa Fabienne, Chauveau, Antoine, Treyer, Andrea, Reinehr, Michael, Oufir, Mouhssin, Duong, Elisa, Potterat, Olivier, Hamburger, Matthias, Simões-Wüst, Ana Paula, University of Zurich, and Simões-Wüst, Ana Paula
- Subjects
Pharmacology ,1602 Analytical Chemistry ,3002 Drug Discovery ,Organic Chemistry ,3003 Pharmaceutical Science ,2707 Complementary and Alternative Medicine ,Pharmaceutical Science ,610 Medicine & health ,Analytical Chemistry ,3004 Pharmacology ,Complementary and alternative medicine ,10049 Institute of Pathology and Molecular Pathology ,1313 Molecular Medicine ,Drug Discovery ,Molecular Medicine ,10026 Clinic for Obstetrics ,1605 Organic Chemistry - Abstract
The placental passage of protopine was investigated with a human ex vivo placental perfusion model. The model was first validated with diazepam and citalopram, 2 compounds known to cross the placental barrier, and antipyrine as a positive control. All compounds were quantified by partially validated U(H)PLC-MS/MS bioanalytical methods. Protopine was transferred from the maternal to the fetal circuit, with a steady-state reached after 90 min. The study compound did not affect placental viability or functionality, as glucose consumption, lactate production, and beta-human chorionic gonadotropin, and leptin release remained constant. Histopathological evaluation of all placental specimens showed unremarkable, age-appropriate parenchymal maturation with no pathologic findings.
- Published
- 2022