1. Improving indigenous Vietnamese Black Rabbit frozen sperm quality: the role of glycine and sperm selection methods
- Author
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Tran Thi Thanh Khuong, Nguyen Lam Khanh Duy, Nguyen Thuy Hang, Phan Kim Ngoc, and Duong Nguyen Duy Tuyen
- Subjects
cryopreservation ,glycine ,indigenous black rabbit ,rabbit sperm ,swim-up ,Animal culture ,SF1-1100 ,Veterinary medicine ,SF600-1100 - Abstract
Rabbit sperm are known to undergo damage during both cryopreservation and thawing, leading to decreased viability, motility and membrane integrity. Glycine can protect sperm and reduce damage during freezing. Swim-up is a simple semen processing method for selecting good motile sperm. The study evaluated the effect of the swim-up method and glycine with different concentrations supplemented to the frozen medium. Three indigenous black rabbits were selected for semen collection by artificial vagina. Next, semen was selected by swim-up method and diluted with glycine-added frozen medium. The samples were then transferred to 0.5 mL straws, cooled to 15°C and 5°C, placed in liquid nitrogen vapour, and finally placed directly into liquid nitrogen (-196°C). The samples were thawed and evaluated for sperm quality. The results showed that the medium supplemented with 10mM glycine in combination with swim-up method for 30 min gave the best results and was significantly different from the remaining concentrations (P
- Published
- 2023
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