Search

The microbiome plays a role in maintaining the balance of the environment. However, the microbiome in the ecosystem has been affected by climate and environmental changes. The human activity that interferes with the balance of nature affects the composition of the microbiome in both the environment and the human body. Climate change drastically influences different environmental cycles. The microbiome responds and adapts to changes in ecological conditions, which results in a change in the balance of the microbiome in nature. It will adversely affect the microbiome imbalance in the human body, which affects the health of human. Therefore, this article will consider a related cycle of effects and the effects of human activity on the environment and the microbiome. The article's perspective should provide a clearer view of the effects of human activity on the changes in the gut microbiome. By humans - because humans - for humans, that is a fitting description of the disease cycle affected by the changes in the gut microbiome. Human activity results in changes in the composition of the soil microbiome and thus the quality and nutrients of food change as well. Changes in the quality of nutrients in the food consumed will cause an imbalance in the intestinal microbiome.

Background and aimsA combined eel and soy-based tempe (CEST) flour is rich in nutrients, especially its high amino acid content in which bioactive peptides (BPs) are expected to be found. Hence, this research aimed to identify the BPs of CEST flour and CEST supplementation’s effect on improving nutritional status biomarkers by ameliorating serum protein, hemoglobin, and IGF-1 of malnourished rats.MethodsCEST flour with a ratio of eel and soy-based tempe of 1:3.5 was produced by applying the oven drying method. Amino acid sequences from six BPs were analyzed using a protein sequencer and spectrometer-electrospray ionization (MS-ESI). A total of thirty malnourished male Rattus norvegicus aged 3–4 weeks were given low-protein (LP; 4% w/w protein) diet treatment for 4 weeks. Afterward, rats were divided into 3 groups of 10 rats. Group A and B remained on a low-protein diet for 4 weeks, receiving an LP diet and getting doses of CEST of 100 and 200 mg/kg BW, respectively, via oral. Group C or control was given a Normal-protein (NP) diet (23% w/w of protein) and was allowed to feed ad libitum during the trial period without a dose of CEST.ResultsSix bioactive peptides were found, with WMGPY being the most abundant, along with a DPPH radical scavenging activity of 5.0 mg/mL. The results showed that serum protein, hemoglobin, and IGF-1 of group B were significantly higher compared to groups A and C (p = 0.0021). CEST dose of 200 mg/kg BW was more effective to increase serum levels of protein (p = 0.0052), hemoglobin, and IGF-1 (p < 0.0001) compared to a 100 mg/kg BW dose.ConclusionThis indicates that the CEST flour has six bioactive peptides, which may contribute to the improvement of nutritional status biomarkers. To establish its potential impact, a human clinical study is urgently needed.