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The article considers the phenomenon of self-organization and academic engagement of university students. The study aims at identifying the nature of interrelation of selforganization as the highest level of self-regulation, academic engagement and a number of specific regulation processes and personality attributes. The study revealed that students with a high level of self-organization show a more developed engagement into an activity, metacognitive engagement, self-efficacy and systemic reflection; academic self-motivation proves to dominate. Students’ engagement with educational and training activities has positive correlations with self-regulation, meta-cognitive involvement, self-efficacy, systemic reflection and quasi-reflection. Forward and backward linkages between engagement and particular components of academic engagement have been observed.

In this paper, we study a system of nonlinear equations on a square graph related to the affine algebra A(1)1 . This system is the simplest representative of the class of discrete systems corresponding to affine Lie algebras. We find the Lax representation and construct hierarchies of higher symmetries. In neighborhoods of singular points ⋋ = 0 and ⋋ = ∞, we construct formal asymptotic expansions of eigenfunctions of the Lax pair and, based on these expansions, find series of local conservation laws for the system considered.

The article is devoted to the actual problem of improving the veterinary and sanitary state of the dairy industry. Satisfactory quality and food safety of products is a necessary minimum of consumer requirements. Searches for ways to improve production and working conditions will always remain relevant for dairy industry, and for any other alike. The following methods: general logic, observation, comparison, practical methods were used in the work. In the course of the work, it was revealed that the implementation of working procedures, sanitary audits, visualization with the help of «lessons on one sheet» help to improve the veterinary and sanitary conditions of dairy production. The practical proposal is to introduce working procedures, «lessons on one sheet», as well as regular sanitary audits everywhere to improve the sanitary and hygienic condition of enterprises.

Currently, an urgent task for food enterprises of the Russian Federation is to improve the quality and safety of their products through the introduction of a system for analyzing critical control points (HACCP). This is important not only for the health of the population, but also for enhancing the advantages of products. The article presents materials concerning implementation of the principles of HASSP on the enterprise JSC «MOROZKO». Theoretical aspects of system of HASSP are given, problems are analyzed and recommendations about their minimization in the conditions of implementation of technical regulations of the Customs union 021/2011 «About safety of food products» are developed. Data of sanitary and microbiological control at veterinary and sanitary examination of the cooled and frozen meat of different software suppliers are provided to the normalized indicators in compliance with normative requirements of meat and meat products. Need of carrying out monitoring of microbiological indicators for critical control points is proved. On the basis of monitoring of microbiological indicators of raw materials, water, air, the equipment, overalls, hands of workers the assessment of risk of microbial contamination of finished goods by pathogenic microorganisms and microorganisms - activators of damage of raw materials and products is carried out. The quantitative composition of microflora was determined during air monitoring in the workshops of the enterprise. Comparative analysis of the influence of disinfection of different types of the equipment on its sanitary states by washout method from the equipment is carried out.

The article presents the results of longstanding field studies (2000-2020) of steppe vegetation and demutation processes of fallow lands in the Altai region of the Republic of Khakassia. The aim of the work is to identify and determine the area of steppe landscapes and demutation areas in the structure of the lands of the Altai region. For this, a land use map of the Altai region was created, the areas of land occupied by steppe vegetation and fallow lands at different stages of restoration were calculated, demutational areas susceptible to overgrowing with trees and shrubs were identified. Based on geobotanical researches, cartographic materials, visual interpretation of Sentinel-2 satellite images of the visible L2A range (2018-2020), a GIS-project was created, including a cluster of raster data, thematic vector layers: water bodies, agricultural lands, vegetation, etc. It allows you to visualize information about the state and use of agricultural lands and vegetation in the form of maps of different thematic scope. It was searched out that the part of steppe landscapes in the total area of the Altai region is 32.2%. In the main, in arable sandy steppes that are not zonal for Khakassia have been preserved. True large bunchgrass and meadow steppes have been preserved in separate fragments and represent all stages of anthropogenous fracturing (various pasture modifications of these steppes). In the study area in the structure of agricultural lands 56860 hectares of agricultural lands belongs to abandoned lands at different stages of restoration, and only 20% postagrogenic lands of which are at the IV stage of restoration (compact-tussock or truly cespitous grasses). The process of restoration of steppe vegetation is accompanied by overgrowing with Ulmus pumila L.

A case study of nodular mastocytosis is described. Tacrolimus was used to treat skin manifestations of mastocytosis.

The article describes in chronological order the accumulated «negative» entropy indices of the Russian people from 1900 to 1917, which led to the 1917 revolution. The revolution in Russia was caused by the negative change of the society’s internal energy and inadequate regulation actions of the tsarist autocracy. The government swiftly destroyed the patriarchal structure that was built for centuries and did not offer any better solutions, which led to the deterioration of the economic state of the nation. People, tired of war, did not accept slogan of the elite - «fight to the finish». Even the military elite conspired to overthrow the autocracy, while the part that backed the government - White movement - was unsuccessful both in the revolution and the civil war. The February Revolution of 1917 is not the result of the Bolsheviks’ victory, but the conspiracy of generals. Analytical calculations of the entropy indices as a «chaos» theory proves the incontestability of these events.

The possibility of the occurrence of seropositive reactions against four pathogens in two feline species (Pallas cat (Otocolobus manul) and domestic cat (Felis catus)) and six species of their potential prey was studied. Blood serum was analyzed for the presence of antibodies to the pathogens by enzyme immunoassay. The occurrence pattern of influenza A virus in manuls was the largest among tested pathogens (22%, n = 18). In this case, positive animals were not discovered among domestic cats. Antibodies against Coxiella burnetii were registered only in domestic cats (3.6%, n = 55). Antibodies against Toxoplasma gondii were found in wild and domestic animals: domestic cats (14.7%, n = 61), Pallas’ cats (9.1%, n = 18), and their prey (3.7%, n = 273). The occurrence of Trichinella sp. was comparatively low in domestic cats (1.6%, n = 61); among prey, antibodies to the parasite were found only in one species, the transbaikal hamster (Cricetulus pseudogriseus) (10.9%, n = 119).

Coccidiostatics are used in poultry farms to prevent and treat eimeriosis, and antibiotics are used to reduce pathogenic microflora, which negatively affects the gut microbiome of birds. In some poultry farms, probiotics are used to correct the gut microbiome of birds with eimeriosis. The use of prebiotics for this purpose has been little studied, although they can also serve as an alternative to antibiotic therapy. The work is devoted to studying the effect of combined and separate administration of chemical coccidiocide and lactulose-containing prebiotic to broilers on the intensity of eimeriosis invasion caused by Eimeria tenella, as well as on the number of mesophilic aerobic and facultative anaerobic microorganisms, including bacteria of the genus Bifidobacterium, Lactobacterium, Clostridium and Escherichia in cecum of 28-day-old broilers. The results showed that on average, for the entire period of the experiment, the lowest intensity of eimeriosis invasion was in the groups where coccidiocide and prebiotic were used: 17.50 ± 5.91 and 17.32 ± 5.67 thousand oocysts/g respectively. In the group where their combined purpose was, the intensity was 27.41 ± 8.13, which was slightly lower than in the infected control - 29.21 ± 8.70 thousand oocysts/g. The introduction of a prebiotic into the broiler diet allowed increasing the number of native bacteria of the genera Bifidobacterium and Lactobacterium, which reduced the number of opportunistic microorganisms such as Escherichia and Clostridium.

Interdisciplinary physical, chemical and planktonic studies in 1998 showed that the growth in anthropogenic inputs over the previous 25 years has led to an increase in nitrate, nitrite, ammonium and total suspended matter content, and to a decrease in oxygen concentration of the near bottom layer in Sevastopol Bay. The situation has been exacerbated by the construction of seawalls narrowing the exit channel of the Bay. There has also been a marked decline in species diversity, in zoo- and ichthyoplankton abundance and an increase in mortality of planktonic organisms. The result is that Sevastopol Bay has lost its importance as a fishery.

To develop highly sensitive sandwich technique for identification of surface hepatitis B virus antigen (HBsAg) in serum and analyse of possible improvement of solid phase for immunoenzyme sandwich technique of HBsAg identification through variation of pH-dependent sorption of monoclonal antibodies on the surface of immune plates.Calibration curves for identification of HBsAg in sandwich techniques using 36 possible binary combinations of monoclonal antibodies of our panel (including high affinity antibodies to HBsAg produced by 6 hybridomas) were compared. Immobilization of antibodies on solid phase (by passive sorption) was performed at different pH values (2.8, 7.5, and 9.5).Analysis of panel of antibodies to HBsAg produced by 6 hybridomas revealed pH-dependent monoclonal antibodies (18C8), which immobilization at low pH values together with detecting antibodies F4F3 allowed to greatly improve sensitivity of the sandwich technique. Minimal credibly detectable concentration of HBsAg in sera of persons infected with hepatitis B virus was 0.013 - 0.017 ng/ml. Validation of sandwich technique was performed on certified panel of serum samples with various concentrations of HBsAg (different serotypes).Highly sensitive sandwich technique for detection of HBsAg was developed. It was shown that analysis of panel of monoclonal antibodies on pH-dependence could be used as simple methodical approach for optimization of immunoenzyme sandwich techniques for detection of different antigens.

The content of triglycerides (TG), total cholesterol (TC) and the level of 5 categories of lipid-peroxidation (LP) products were investigated in blood serum of 27 patients with atherogenic dislipidemic disorders. Multiple regression analysis was made for the dependence of the concentrations of TG and TC on a level of the LP circulating products. It was found that the parameters of the LP products' content in blood serum can be used to calculate the TG and TC concentrations, i.e. they are a system of indirect markers of a pronouncement degree of dislipidemic disorders.

Complex anthropometric and laboratory investigation of 30 healthy men produced evidence that body mass index and the lipoperoxidation product contents in blood sera may be regarded as associated indirect markers of neutrophiles' functional status and immunoglobulinemia.

Examinations of 19 patients with dislipedemias (types IIA, IIB, III and IV) led to establishing the interrelations between the indices of the antigenic composition of circulating blood leukocytes, the total leukocyte count, the circulating neutrophil count and a severity of dislipidemia disorders (according to the parameters of the atherogenic coefficient and the beta-LP content). It was found that the assessment of polymorphism of circulating leukocytes could be used as an indirect evaluation of a severity of dislipidemia disorders.

The interaction between two different monoclonal antibodies (Mabs) and their corresponding bispecific antibodies (Babs) with immobilized antigens was investigated using an optical biosensor (IAsys). The analyzed panel of affinity-purified antibodies included two parental Mabs (one of which was specific to human IgG (hIgG), and another one to horseradish peroxidase (HRP)), as well as Babs derived thereof (anti-hIgG/HRP). Babs resulting from the fusion of parental hybridomas bear two antigen-binding sites toward two different antigens and thus may interact with immobilized antigen through only one antigen-binding site (monovalently). Using an IAsys biosensor this study shows that the bivalent binding of Mabs predominates over the monovalent binding with immobilized HRP, whereas anti-hIgG parental Mabs were bound monovalently to the immobilized hIgG. The observed equilibrium association constant (K(ass)) values obtained in our last work [1] by solid-phase radioimmunoassay are consistent with those constants obtained by IAsys. The K(ass) of anti-HRP Mabs was about 50 times higher than that of anti-HRP shoulder of Babs. The dissociation rate constant (k(diss)) for anti-HRP shoulder of Babs was 21 times higher than k(diss) for anti-HRP Mabs. The comparison of the kinetic parameters for bivalent anti-HRP Mabs and Babs derived from anti-Mb/HRP and anti-hIgG/HRP, allowed to calculate that 95% of bound anti-HRP Mabs are bivalently linked with immobilized HRP, whereas only 5% of bound anti-HRP Mabs are monovalently linked. In general, the data obtained indicate that Babs bearing an enzyme-binding site may not be efficiently used instead of traditional antibody-enzyme conjugates in the case of binding of bivalent Mabs.

Results of investigations on the composition and quantitative distribution of zooplankton of the eastern Mediterranean Sea were reviewed for the period 1950-1980. The studies reviewed comprise more than 250 stations (with about 2000 samples) mainly from the expeditions carried out by the Institute of Biology of the Southern Seas (IBSS), attached to the National Academy of Science of the Ukraine. Many of these stations are located in poorly studied deep-water regions. For comparison purposes data from the literature on the coastal regions were also used.

Based on the results of clinico-bacteriological and immunological studies carried out in the early neonatal period in 65 neonates afflicted with pyoinflammatory diseases and in their mothers, diagnostic criteria for intrauterine and early neonatal infection were elaborated. The significance played in this process of the character and level of microbial colonization of the amniotic fluid, placenta, labor tract of the mother, intrauterine antigenic stimulation of the fetus, changes in the peripheral blood and inflammatory alterations in the placenta was defined. Clinical, bacteriological and immunological prognostic criteria for the risk of the development of pyoinflammatory diseases in neonates were elaborated.

A study was made of the establishment of biocenosis in the early neonatal period in healthy full-term neonates born to healthy mothers and in children belonging to a group at risk with an aggravated anamnesis but with no history of pyoinflammatory diseases. The study was related to bacterial colonization of the mother, amniotic fluid and placenta as well as to the immune status of the lying in woman and fetus. In 37% of children belonging to the group at risk, the minimal signs of intrauterine infection were discovered.

Aim of studdy: To assess the frequency of practical application of different basic drugs in rheumatoid arthritis (RA). Material and methods: Tlxe study was conducted basing of questionner of pts and analysis of ycases by randomized sampling among 103 consequent pts (M:F= 13:90) with reliable RA (ARA, 1987) in rheumatologic department of Clinical Hospital Nol in Ekaterinburg. 74% of pts under study demonstrated systemic manifestations: anemia (in 47 pts), lymphadenopathy (in 34), rheumatoid nodules (in 15), Sjogren s syndrome (in 4), nephropathy (in 4), vascular disturbances including Raynaud s phenomenon, capillarites (by 1 pt). Results: In the course of disease basic therapy was prescribed to 88 out of103 (85.4%) pts and one and the same patient could take different basic drugs. Aminochinoline drugs prevailed, after them more frequent were immunodepressants and gold preparations. More rarely pts had sulfasalazin, cuprenil and wobenzym. In general, in 133 out of 184 cases of prescribing basic drugs they were canceled. The reason for cancellation were: prevalently absence of the drug in the pharmaceutical stores (in 48 cases) averagely in 8 months of taking the drug; then they insufficient efficacy (44 cases) averagely in 1.3 year. In 18 cases pts themselves stopped treatment averagely in 3.5 months of drug taking. Conclusion: In the majority of cases of basic drugs cancellation in RA the cause is their absence in sail especially on free of charge prescription. Cases ofself-cancellation of the drug demonstrate the need of explaining to pts the necessity> of long-term taking disease-modifying drugs.

Biological fluids (blood serum, cerebrospinal fluid) and brain extract obtained from patients with Alzheimer's disease (6 cases), simple senile dementia (5 cases), and other atrophic processes (1 case) were administered into the nutrient media of human fetal brain tissue culture. It was found that brain tissue extract obtained from patients with Alzheimer's disease stimulated accumulation of filamentous material in neurons while the CSF induced the formation of orderly filamentous rings. The effect of blood serum was characterized by nonspecific manifestations in the form of accumulation of structurally heterogeneous lamellar corpuscles.

Upon cultivation of the brain tissue and skin-muscle tissues of human embryos, a polyimide film was used as a lining. The optimal conditions of the preliminary processing of the lining (sterilization, washing) are described. An easy separation of the polyimide film from the media (in the process of tissue treatment for electron microscope studies) facilitates the use of the material for light and electron microscopy without damaging the culture.

Using the method of electron microscopy microtubules and microfilaments were examined in the brain cells of human embryos taken from healthy and schizophrenic women as a result of medical abortions. The brain cells of the embryos obtained from the schizophrenic women showed greater numbers (as compared with normal) of the organelles of both types: the organelles formed accumulations, and lost their correct orientation; the microfilaments contained compact inclusions, and the microtubules were convoluted.