29 results on '"EID, Eltayeb E. M."'
Search Results
2. Targeting SARS-CoV-2 main protease by teicoplanin: a mechanistic insight by docking, MM/GBSA and molecular dynamics simulation
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Azam, Faizul, Eid, Eltayeb E M, and Almutairi, Abdulkarim
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- 2021
- Full Text
- View/download PDF
3. Enhancing the Anti-Leukemic Potential of Thymoquinone/Sulfobutylether-β-cyclodextrin (SBE-β-CD) Inclusion Complexes
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Eid, Eltayeb E. M., primary, Alshehade, Salah Abdalrazak, additional, Almaiman, Amer A., additional, Kamran, Sareh, additional, Lee, Vannajan Sanghiran, additional, and Alshawsh, Mohammed Abdullah, additional
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- 2023
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4. Characterization of Thymoquinone-Sulfobutylether-β-Cyclodextrin Inclusion Complex for Anticancer Applications
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Eid, Eltayeb E. M., primary, Almaiman, Amer A., additional, Alshehade, Salah Abdalrazak, additional, Alsalemi, Wardah, additional, Kamran, Sareh, additional, Suliman, FakhrEldin O., additional, and Alshawsh, Mohammed Abdullah, additional
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- 2023
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5. Characterization, drug release profile and cytotoxicity of Dentatin-Hydroxypropyl-β-Cyclodextrin complex
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Ashwaq, Al-Abboodi Shakir, Rasedee, Abdullah, Abdul, Ahmad Bustamam, Taufiq-Yap, Yun Hin., Al-Qubaisi, Mothanna Sadiq, and Eid, Eltayeb E. M.
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- 2017
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6. Dispersive solid-phase extraction for simultaneous determination of four amphetamines drugs in urine using gas chromatography–mass spectrometry
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Elboraie, Mahir Z., primary, Elbashir, Abdalla A., additional, Eid, Eltayeb E. M., additional, and Aboul-Enein, Hassan Y., additional
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- 2021
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7. Probiotics supplementation in patients with colorectal cancer: a systematic review of randomized controlled trials
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Dikeocha, Ifeoma Julieth, primary, Al-Kabsi, Abdelkodose Mohammed, additional, Eid, Eltayeb E M, additional, Hussin, Salasawati, additional, and Alshawsh, Mohammed Abdullah, additional
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- 2021
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8. An in-silico analysis of ivermectin interaction with potential SARS-CoV-2 targets and host nuclear importin α
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Azam, Faizul, Taban, Ismail M., Eid, Eltayeb E. M., Iqbal, Muzaffar, Alam, Ozair, Khan, Shamshir, Mahmood, Danish, Anwar, Md Jamir, Khalilullah, Habibullah, and Khan, M. U.
- Subjects
embryonic structures - Abstract
Ivermectin (IVM) is a broad-spectrum antiparasitic agent, having inhibitory potential against wide range of viral infections. It has also been found to hamper SARS-CoV-2 replication in vitro, and its precise mechanism of action against SARS-CoV-2 is yet to be understood. IVM is known to interact with host importin (IMP)α directly and averts interaction with IMPβ1, leading to the prevention of nuclear localization signal (NLS) recognition. Therefore, the current study seeks to employ molecular docking, molecular mechanics generalized Born surface area (MM-GBSA) analysis and molecular dynamics simulation studies for decrypting the binding mode, key interacting residues as well as mechanistic insights on IVM interaction with 15 potential drug targets associated with COVID-19 as well as IMPα. Among all COVID-19 targets, the non-structural protein 9 (Nsp9) exhibited the strongest affinity to IVM showing −5.30 kcal/mol and −84.85 kcal/mol binding energies estimated by AutoDock Vina and MM-GBSA, respectively. However, moderate affinity was accounted for IMPα amounting −6.9 kcal/mol and −66.04 kcal/mol. Stability of the protein-ligand complexes of Nsp9-IVM and IMPα-IVM was ascertained by 100 ns trajectory of all-atom molecular dynamics simulation. Structural conformation of protein in complex with docked IVM exhibited stable root mean square deviation while root mean square fluctuations were also found to be consistent. In silico exploration of the potential targets and their interaction profile with IVM can assist experimental studies as well as designing of COVID-19 drugs. Communicated by Ramaswamy H. Sarma
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- 2020
- Full Text
- View/download PDF
9. Anin-silicoanalysis of ivermectin interaction with potential SARS-CoV-2 targets and host nuclear importin α
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Azam, Faizul, primary, Taban, Ismail M., additional, Eid, Eltayeb E. M., additional, Iqbal, Muzaffar, additional, Alam, Ozair, additional, Khan, Shamshir, additional, Mahmood, Danish, additional, Anwar, Md Jamir, additional, Khalilullah, Habibullah, additional, and Khan, M. U., additional
- Published
- 2020
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- View/download PDF
10. An in-silico analysis of ivermectin interaction with potential SARS-CoV-2 targets and host nuclear importin α.
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Azam, Faizul, Taban, Ismail M., Eid, Eltayeb E. M., Iqbal, Muzaffar, Alam, Ozair, Khan, Shamshir, Mahmood, Danish, Anwar, Md Jamir, Khalilullah, Habibullah, and Khan, M. U.
- Published
- 2022
- Full Text
- View/download PDF
11. Probiotics supplementation in patients with colorectal cancer: a systematic review of randomized controlled trials.
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Dikeocha, Ifeoma Julieth, Al-Kabsi, Abdelkodose Mohammed, Eid, Eltayeb E M, Hussin, Salasawati, and Alshawsh, Mohammed Abdullah
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ONLINE information services ,LENGTH of stay in hospitals ,SYSTEMATIC reviews ,SURGICAL complications ,PROBIOTICS ,DIETARY supplements ,COLORECTAL cancer ,CANCER patients ,HUMAN microbiota ,QUALITY of life ,DESCRIPTIVE statistics ,RESEARCH funding ,MEDLINE ,LACTOBACILLUS - Abstract
Context Colorectal cancer (CRC) is a leading cause of cancer deaths. Recently, much attention has been given to the microbiome and probiotics as preventive and therapeutic approaches to CRC and the mechanisms involved. Objectives To interpret the findings of randomized controlled trials (RCTs) of probiotics relative to patients with CRC and to outline challenges of and future directions for using probiotics in the management and prevention of CRC. Data sources Web of Science, PubMed, ProQuest, Wile,y and Scopus databases were searched systematically from January 17–20, 2020, in accordance with PRISMA guidelines. Study selection Primacy RCTs that reported the effects of administration to patients with CRC of a probiotic vs a placebo were eligible to be included. Data Extraction The studies were screened and selected independently by 2 authors on the basis of prespecified inclusion and exclusion criteria. The data extraction and risk-of-bias assessment were also performed independently by 2 authors. Results A total of 23 RCTs were eligible for inclusion. Probiotics supplementation in patients with CRC improved their quality of life, enhanced gut microbiota diversity, reduced postoperative infection complications, and inhibited pro-inflammatory cytokine production. The use of certain probiotics in patients with CRC also reduced the side effects of chemotherapy, improved the outcomes of surgery, shortened hospital stays, and decreased the risk of death. Bifidobacteria and Lactobacillus were the common probiotics used across all studies. Conclusion Probiotics have beneficial effects in patients with CRC regardless of the stage of cancer. There is an opportunity for probiotics to be used in mainstream health care as a therapy in the fight against CRC, especially in early stages; however, larger clinical trialsof selected or a cocktail of probiotics are needed to confirm the efficacy, dosage, and interactions with chemotherapeutics agents. Systematic Review Registration PROSPERO registration no. CRD42020166865. [ABSTRACT FROM AUTHOR]
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- 2022
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12. An in-silicoanalysis of ivermectin interaction with potential SARS-CoV-2 targets and host nuclear importin α
- Author
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Azam, Faizul, Taban, Ismail M., Eid, Eltayeb E. M., Iqbal, Muzaffar, Alam, Ozair, Khan, Shamshir, Mahmood, Danish, Anwar, Md Jamir, Khalilullah, Habibullah, and Khan, M. U.
- Abstract
AbstractIvermectin (IVM) is a broad-spectrum antiparasitic agent, having inhibitory potential against wide range of viral infections. It has also been found to hamper SARS-CoV-2 replication in vitro,and its precise mechanism of action against SARS-CoV-2 is yet to be understood. IVM is known to interact with host importin (IMP)α directly and averts interaction with IMPβ1, leading to the prevention of nuclear localization signal (NLS) recognition. Therefore, the current study seeks to employ molecular docking, molecular mechanics generalized Born surface area (MM-GBSA) analysis and molecular dynamics simulation studies for decrypting the binding mode, key interacting residues as well as mechanistic insights on IVM interaction with 15 potential drug targets associated with COVID-19 as well as IMPα. Among all COVID-19 targets, the non-structural protein 9 (Nsp9) exhibited the strongest affinity to IVM showing −5.30 kcal/mol and −84.85 kcal/mol binding energies estimated by AutoDock Vina and MM-GBSA, respectively. However, moderate affinity was accounted for IMPα amounting −6.9 kcal/mol and −66.04 kcal/mol. Stability of the protein-ligand complexes of Nsp9-IVM and IMPα-IVM was ascertained by 100 ns trajectory of all-atom molecular dynamics simulation. Structural conformation of protein in complex with docked IVM exhibited stable root mean square deviation while root mean square fluctuations were also found to be consistent. In silicoexploration of the potential targets and their interaction profile with IVM can assist experimental studies as well as designing of COVID-19 drugs. Communicated by Ramaswamy H. Sarma
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- 2022
- Full Text
- View/download PDF
13. CYTOTOXICITY OF IRON DOPED SULFATED ZIRCONIA NANOPARTICLES: SYNTHESIS, CHARACTERIZATION AND IN VITRO STUDY.
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Abdullah, Qasim Khlaif, Al-Fahdawi, Mohamed Qasim, Hammad, Ruaa Tareq, Eid, Eltayeb E. M., Al-Qubaisi, Mothanna Sadiq, and Rasedee, Abdullah
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CELL-mediated cytotoxicity ,ZIRCONIUM oxide ,NANOPARTICLES ,ANTINEOPLASTIC agents ,X-ray photoelectron spectroscopy - Abstract
Nanostructured iron-doped sulfated zirconia was prepared via the precipitation and dry spray technique followed by calcination at 700oC for 5h. The physicochemical characterization of these nanomaterials was performed using X-ray diffraction, X-ray photoelectron spectroscopy and transmission electron microscopy. The average diameter of iron-doped sulfated zirconia nanoparticles is estimated to be 29/nm. The anti-cancer effects of iron-doped sulfated zirconia nanoparticles were examined on the human breast cancer MDA-MB-231, colon cancer HT29 and liver cancer HepG2 cell lines using the MTT (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The study showed that iron-doped sulfated zirconia nanoparticles have potential to be developed into an anti-cancer compound. [ABSTRACT FROM AUTHOR]
- Published
- 2020
14. Zerumbone binding to estrogen receptors: an in-silico investigation
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Eid, Eltayeb E. M., primary, Azam, Faizul, additional, Hassan, Mahmoud, additional, Taban, Ismail M., additional, and Halim, Mohammad A., additional
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- 2018
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15. Proteomic profile of acute myeloid leukaemia: a review update
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Almaiman, Amer Abdulrahman, Abdullah, Rasedee, Abdul, Ahmad, Allauddin, Zeenathul, Eid, Eltayeb E. M., Saghir, Sultan Ayesh Mohammed, Almaiman, Amer Abdulrahman, Abdullah, Rasedee, Abdul, Ahmad, Allauddin, Zeenathul, Eid, Eltayeb E. M., and Saghir, Sultan Ayesh Mohammed
- Abstract
Proteome analysis is a complex and dynamic process that encompasses several analytical platforms that include protein sequencing, structural or expression proteomics, protein modification, sub-cellular protein localization, protein-protein interaction and biological functional proteomics. In fact, expression proteomics is extensively applied in a majority of biomarker detection studies because it provides a detailed overview of differentially expressed proteins in cellular pathways and disease processes. Proteomics are also effective and dynamic in protein-protein interactions and cross-talks between interacting molecules of the cell. Proteomics has evolved into a crucial tool used to investigate the biochemical changes that possibly lead to development of cancer biomarkers. This review draws attention to the progress and advancements in cancer proteomics technology with the aim of simplifying the understanding of the mechanisms underlying the disease and to contribute to detection of biomarkers in addition to the development of novel treatments. Given that proteome is a dynamic entity of cellular functions in health and disease, it is capable of reflecting the immediate environmental state of cells and tissues as shown in this review. The review shows the possibility of elucidating the pathophysiology of acute myeloid leukaemia (AML) through proteome expressions, thus confirming the viability of proteome analysis in profiling AML.
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- 2016
16. Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin
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Al-Qubaisi, Mothanna Sadiq, Abdullah, Rasedee, Flaifel, Moayad Husein, Ahmad, Sahrim, Al-Ali, Samer Hussein, Hussein, Mohd. Zobir, Eid, Eltayeb E. M., Zainal, Zulkarnain, Mohd Saeed, Ilowefah, Muna, Fakurazi, Sharida, Mohd Isa, Norhaszalina, El Zowalaty, Mohamed Ezzat, Al-Qubaisi, Mothanna Sadiq, Abdullah, Rasedee, Flaifel, Moayad Husein, Ahmad, Sahrim, Al-Ali, Samer Hussein, Hussein, Mohd. Zobir, Eid, Eltayeb E. M., Zainal, Zulkarnain, Mohd Saeed, Ilowefah, Muna, Fakurazi, Sharida, Mohd Isa, Norhaszalina, and El Zowalaty, Mohamed Ezzat
- Abstract
In this study, in vitro cytotoxicity of nickel zinc (NiZn) ferrite nanoparticles against human colon cancer HT29, breast cancer MCF7, and liver cancer HepG2 cells was examined. The morphology, homogeneity, and elemental composition of NiZn ferrite nanoparticles were investigated by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. The exposure of cancer cells to NiZn ferrite nano-particles (15.6-1,000 μg/mL; 72 hours) has resulted in a dose-dependent inhibition of cell growth determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The quantification of caspase-3 and -9 activities and DNA fragmentation to assess the cell death pathway of the treated cells showed that both were stimulated when exposed to NiZn ferrite nanoparticles. Light microscopy examination of the cells exposed to NiZn ferrite nanoparticles demonstrated significant changes in cellular morphology. The HepG2 cells were most prone to apoptosis among the three cells lines examined, as the result of treatment with NiZn nanoparticles. In conclusion, NiZn ferrite nanoparticles are suggested to have potential cytotoxicity against cancer cells.
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- 2013
17. Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release
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Al-Qubaisi, Mothanna Sadiq, Abdullah, Rasedee, Flaifel, Moayad Husein, Ahmad, Sahrim, Al Ali, Samer Hassein, Hussein, Mohd Zobir, Zainal, Zulkarnain, Alhassan, Fatah H., Yap, Taufiq Yun Hin, Eid, Eltayeb E. M., Arbab, Ismail Adam, Al-Asbahi, Bandar A., Webster, Thomas J., El Zowalaty, Mohamed Ezzat, Al-Qubaisi, Mothanna Sadiq, Abdullah, Rasedee, Flaifel, Moayad Husein, Ahmad, Sahrim, Al Ali, Samer Hassein, Hussein, Mohd Zobir, Zainal, Zulkarnain, Alhassan, Fatah H., Yap, Taufiq Yun Hin, Eid, Eltayeb E. M., Arbab, Ismail Adam, Al-Asbahi, Bandar A., Webster, Thomas J., and El Zowalaty, Mohamed Ezzat
- Abstract
The long-term objective of the present study was to determine the ability of NiZn ferrite nanoparticles to kill cancer cells. NiZn ferrite nanoparticle suspensions were found to have an average hydrodynamic diameter, polydispersity index, and zeta potential of 254.2±29.8nm, 0.524 ±0.013, and -60±14mV, respectively. We showed that NiZn ferrite nanoparticles had selective toxicity towards MCF-7, HepG2, and HT29cells, with a lesser effect on normal MCF 10A cells. The quantity of Bcl-2, Bax, p53, and cytochrome C in the cell lines mentioned above was determined by colorimetric methods in order to clarify the mechanism of action of NiZn ferrite nanoparticles in the killing of cancer cells. Our results indicate that NiZn ferrite nanoparticles promote apoptosis in cancer cells via caspase-3 and caspase-9, downregulation of Bcl-2, and upregulation of Bax and p53, with cytochrome C translocation. There was a concomitant collapse of the mitochondrial membrane potential in these cancer cells when treated with NiZn ferrite nanoparticles. This study shows that NiZn ferrite nanoparticles induce glutathione depletion in cancer cells, which results in increased production of reactive oxygen species and eventually, death of cancer cells.
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- 2013
18. Inclusion Complex of Zerumbone with Hydroxypropyl-β-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8/BID Cleavage Switch and Modulating Bcl2/Bax Ratio
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Muhammad Nadzri, Nabilah, primary, Abdul, Ahmad Bustamam, additional, Sukari, Mohd Aspollah, additional, Abdelwahab, Siddig Ibrahim, additional, Eid, Eltayeb E. M., additional, Mohan, Syam, additional, Kamalidehghan, Behnam, additional, Anasamy, Theebaa, additional, Ng, Kuan Beng, additional, Syam, Suvitha, additional, Arbab, Ismail Adam, additional, Rahman, Heshu Sulaiman, additional, and Ali, Hapipah Mohd, additional
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- 2013
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19. Liquid chromatography-tandem mass spectroscopic method for the determination of zerumbone in human plasma and its application to pharmacokinetics
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Eid, Eltayeb E. M., primary, Bustamam Abdul, Ahmad, additional, Rasedee, A., additional, Suliman, Fakhr Eldin O., additional, Sukari, Mohd A., additional, and Fatah, Safa A., additional
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- 2011
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20. Inclusion Complex of Zerumbone with Hydroxypropyl-β-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8/BID Cleavage Switch and Modulating Bcl2/Bax Ratio.
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Nadzri, Nabilah Muhammad, Abdul, Ahmad Bustamam, Sukari, Mohd Aspollah, Abdelwahab, Siddig Ibrahim, Eid, Eltayeb E. M., Mohan, Syam, Kamalidehghan, Behnam, Anasamy, Theebaa, Ng, Kuan Beng, Syam, Suvitha, Arbab, Ismail Adam, Rahman, Heshu Sulaiman, and Ali, Hapipah Mohd
- Subjects
APOPTOSIS ,BIOLOGICAL assay ,CELL lines ,HEPATOCELLULAR carcinoma ,LIVER ,MEDICINAL plants ,MITOCHONDRIA ,RESEARCH funding ,T-test (Statistics) ,TOXICITY testing ,PHYTOCHEMICALS ,IN vitro studies - Abstract
Zerumbone (ZER) isolated from Zingiber zerumbet was previously encapsulated with hydroxypropyl-β-cyclodextrin (HPβCD) to enhance ZER's solubility in water, thus making it highly tolerable in the human body. The anticancer effects of this new ZER-HPβCD inclusion complex via apoptosis cell death were assessed in this study for the first time in liver hepatocellular cells, HepG2. Apoptosis was ascertained by morphological study, nuclear stain, and sub-Gl cell population accumulation with G2/M arrest. Further investigations showed the release of cytochrome c and loss of mitochondrial membrane potential, proving mitochondrial dysfunction upon the ZER-HPβCD treatment as well as modulating proapoptotic and anti-apototic Bcl-2 family members. A significant increase in caspase 3/7, caspase 9, and caspase 8 was detected with the depletion of BID cleaved by caspase 8. Collectively, these results prove that a highly soluble inclusion complex of ZER-HPβCD could be a promising anticancer agent for the treatment of hepatocellular carcinoma in humans. [ABSTRACT FROM AUTHOR]
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- 2013
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21. Cardamonin Exerts Antitumor Effect on Human Hepatocellular Carcinoma Xenografts in Athymic Nude Mice through Inhibiting NF-κβ Pathway.
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Badroon, Nassrin, Abdul Majid, Nazia, Al-Suede, Fouad Saleih R., Nazari V., Mansoureh, Giribabu, Nelli, Abdul Majid, Amin Malik Shah, Eid, Eltayeb E. M., and Alshawsh, Mohammed Abdullah
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HEPATOCELLULAR carcinoma ,LIVER cancer ,XENOGRAFTS ,BCL-2 proteins ,BAX protein - Abstract
Cardamonin (CADMN) exerts an in vitro antiproliferative and apoptotic actions against human hepatocellular carcinoma cells (HepG2). This study aimed to investigate the in vivo anti-tumorigenic action of CADMN against human hepatocellular carcinoma xenografts in an athymic nude mice, as well as to study the molecular docking and safety profile of this compound. Acute toxicity study demonstrated that CADMN is safe and well-tolerated up to 2000 mg/kg in ICR mice. Oral administration of 50 mg/kg/day of CADMN in xenografted nude mice showed a significant suppression in tumor growth as compared to untreated control group without pronounced toxic signs. Immunohistochemistry assay showed downregulation of proliferative proteins such as PCNA and Ki-67 in treated groups as compared to untreated control. Additionally, immunofluorescence analysis showed a significant downregulation in anti-apoptotic Bcl-2 protein, whereas pre-apoptotic Bax protein was significantly upregulated in nude mice treated with 25 and 50 mg/kg CADMN as compared to untreated mice. The findings also exhibited down-regulation of NF-κB-p65, and Ikkβ proteins, indicating that CADMN deactivated NF-κB pathway. The molecular docking studies demonstrated that CADMN exhibits good docking performance and binding affinities with various apoptosis and proliferation targets in hepatocellular cancer cells. In conclusion, CADMN could be a potential anticancer candidate against hepatocellular carcinoma. Other pharmacokinetics and pharmacodynamics properties, however, need to be further investigated in depth. [ABSTRACT FROM AUTHOR]
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- 2020
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22. The Potential of Protein Expression Profiles in Categorization Risks for Acute Myeloid Leukemia: A Pilot Study.
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ALMAIMAN, Amer, ABDULLAH, Rasedee, bin ABDUL, Ahmad B., ALLAUDDIN, Zeenathul N., ALAIYA, Ayodele A., EID, Eltayeb E. M., SHINWARI, Zakia, Al JUHANI, Ghada, RASHEED, Walid, BAKSHI, Nasir, OWAIDAH, Tareq, AHMED, Syed O., and ALJURF, Mahmoud
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PROTEIN expression , *PEPTIDE antibiotics , *ACUTE myeloid leukemia , *IMMUNOHISTOCHEMISTRY , *NATURAL immunity , *ADENOCARCINOMA - Abstract
Currently, there are no markers to predict response to acute myeloid leukemia (AML) therapy and patients have to wait for a period of 3-6 months to see treatment response. The study aimed to analyze changes in protein expression in AML cells between different categorization risk groups using proteomics techniques. Six peripheral blood (PB) and six bone marrow (BM) samples at diagnosis and remission times were collected from AML patients. Another Six PB samples were collected from different categories of AML. All samples were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The levels of proteins in patients with AML were compared at different categorization risk, individual response to treatment and clinical characteristics. Twenty-one and 145 differentially expressed proteins were identified with disease progression and risk categories of AML, respectively. Three (3) proteins were noticeably highly expressed out of the range of others proteins by at least 3- fold difference between diagnosis and remission. Two other of proteins were up regulated by more than 10 folds between risk categories of AML. Furthermore, 4 proteins were found to be expressed in one risk category, but were not detectable in other two risk categories. The study showed that a panel of differentially expressed protein profiles might serve as more objective biomarkers for accurate stratification of different risk categories of AML. [ABSTRACT FROM AUTHOR]
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- 2015
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23. Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite.
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Al-Qubaisi MS, Al-Abboodi AS, Alhassan FH, Hussein-Al-Ali S, Flaifel MH, Eid EEM, Alshwyeh HA, Hussein MZ, Alnasser SM, Saeed MI, Rasedee A, and Ibrahim WN
- Abstract
In this study, we formulated Thymoquinone-loaded nanocomposites (TQ-NCs) using high-pressure homogenizer without sodium tripolyphosphate. The TQ-NCs were characterized and their anti-inflammatory determined by the response of the LPS-stimulated macrophage RAW 264.7 cells in the production of nitric oxide, prostaglandin E2, tumor necrosis factor-α, interleukin-6, and interleukin-1β. The physicochemical properties of TQ-NC were determined using different machines. TQ was fully incorporated in the highly thermal stable nanoparticles. The nanoparticles showed rapid release of TQ in the acidic medium of the gastric juice. In medium of pH 6.8, TQ-NC exhibited sustained release of TQ over a period of 100 h. The results suggest that TQ-NC nanoparticles have potential application as parenterally administered therapeutic compound. TQ-NC effectively reduce production of inflammatory cytokines by the LPS-stimulated RAW 264.7 cells, indicating that they have anti-inflammatory properties. In conclusion, TQ-NC nanoparticles have the characteristics of efficient carrier for TQ and an effective anti-inflammatory therapeutic compound., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 The Author(s).)
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- 2022
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24. Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells.
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Al-Fahdawi MQ, Al-Doghachi FAJ, Abdullah QK, Hammad RT, Rasedee A, Ibrahim WN, Alshwyeh HA, Alosaimi AA, Aldosary SK, Eid EEM, Rosli R, Taufiq-Yap YH, Al-Haj NA, and Al-Qubaisi MS
- Subjects
- A549 Cells, Cell Line, Tumor, Cell Survival drug effects, Cell Survival physiology, Dose-Response Relationship, Drug, HT29 Cells, Humans, Inflammation Mediators metabolism, Oxidative Stress physiology, Cytotoxins toxicity, Magnesium Oxide toxicity, Metal Nanoparticles toxicity, Oxidative Stress drug effects, Platinum toxicity
- Abstract
The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinum-doped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30-50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anti-cancer compound., (Copyright © 2021 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Published
- 2021
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25. Inclusion complex of clausenidin with hydroxypropyl-β-cyclodextrin: Improved physicochemical properties and anti-colon cancer activity.
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Al-Abboodi AS, Al-Sheikh WM, Eid EEM, Azam F, and Al-Qubaisi MS
- Abstract
The long-term objective of the present study was to prepare, physicochemically characterize and determine the anticancer of clausenidin/hydroxypropyl-β-cyclodextrin (Clu/HPβCD) inclusion complex. We used differential scanning calorimetry, X-ray diffractometer, fourier transform infrared spectroscopy, ultraviolet-visible spectrophotometer and
13 C and1 H nuclear magnetic resonance followed by in vitro anticancer assays. The orientation and intermolecular interactions of Clausenidin within cyclodextrin cavity were also ascertained by molecular docking simulation accomplished by AutoDock Vina. The guest molecule was welcomed by the hydrophobic cavity of the host molecule and sustained by hydrogen bond between host/guest molecules. The constant drug release with time, and increased solubility were found after successful complexation with HPβCD as confirmed by physicochemical characterizations. Clausenidin had greater cytotoxic effect on colon cancer HT29 cells when incorporated into HPβCD cavity than dissolved in DMSO. Also, from a comparison of cell viability between normal and cancer cells, a reduced side effect was observed. The Clu/HPβCD inclusion complex triggered reactive oxygen species-mediated cytotoxicity in HT29 cells. The inclusion complex-treated HT29 cells showed cell cycle arrest and death by apoptosis associated with caspases activation. The presence of HPβCD seems to aid the anticancer activity of clausenidin., (© 2021 The Author(s).)- Published
- 2021
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26. Characterization of thymoquinone/hydroxypropyl-β-cyclodextrin inclusion complex: Application to anti-allergy properties.
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Al-Qubaisi MS, Rasedee A, Flaifel MH, Eid EEM, Hussein-Al-Ali S, Alhassan FH, Salih AM, Hussein MZ, Zainal Z, Sani D, Aljumaily AH, and Saeed MI
- Subjects
- Animals, Anti-Allergic Agents chemistry, Benzoquinones chemistry, Cell Line, Tumor, Drug Liberation, Gastric Juice chemistry, Histamine metabolism, Interleukin-4 metabolism, Intestinal Secretions chemistry, Rats, Tumor Necrosis Factor-alpha metabolism, 2-Hydroxypropyl-beta-cyclodextrin administration & dosage, Anti-Allergic Agents administration & dosage, Benzoquinones administration & dosage, Drug Delivery Systems
- Abstract
Thymoquinone is an effective phytochemical compound in the treatment of various diseases. However, its practical administration has been limited due to poor aqueous solubility and bioavailability. In this work, we developed a novel inclusion complex of thymoquinone and hydroxypropyl-β-cyclodextrin that features improved solubility and bioactivity. The drug solubility was markedly accelerated in the increasing ratio of hydroxypropyl-β-cyclodextrin to thymoquinone amount. The formation of the thymoquinone/hydroxypropyl-β-cyclodextrin inclusion complex was evidenced using X-ray diffraction, differential scanning calorimetry, thermal gravimetric analysis, Fourier transform infrared, scanning electron microscopy and nuclear magnetic resonance. The release behavior of the complex, as well as of their mixtures, was examined in artificial gastric (pH 1.2) and intestinal (pH 6.8) dissolution media. The formulated complex released the drug rapidly at the initial stage, followed by a slow release. Thermodynamic parameters ΔH, ΔS and ΔG were calculated with temperatures ranging from 20 to 45 °C to evaluate the complexation process. The activity of the inclusion complex was evaluated on IgE-mediated allergic response in rat basophilic leukemia (RBL-2H3) cells by monitoring key allergic mediators. The results revealed that compared with free thymoquinone, the inclusion complex more strongly inhibited the release of histamine, tumor necrosis factor-α, and interleukin-4, and was not cytotoxic at the tested thymoquinone concentrations (0.125-4 μg/mL) indicating the inclusion complex possibly had better antiallergic effects. Our finding suggested that the inclusion complex achieved prolonged action and reduced side-effect of thymoquinone., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
27. Induction of apoptosis in cancer cells by NiZn ferrite nanoparticles through mitochondrial cytochrome C release.
- Author
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Al-Qubaisi MS, Rasedee A, Flaifel MH, Ahmad SH, Hussein-Al-Ali S, Hussein MZ, Zainal Z, Alhassan FH, Taufiq-Yap YH, Eid EE, Arbab IA, Al-Asbahi BA, Webster TJ, and El Zowalaty ME
- Subjects
- Antineoplastic Agents chemistry, Cell Line, Tumor, Cell Proliferation, Cytochromes c analysis, Cytochromes c metabolism, Ferric Compounds chemistry, Glutathione analysis, Glutathione metabolism, Humans, Malondialdehyde analysis, Malondialdehyde metabolism, Membrane Potential, Mitochondrial drug effects, Nickel chemistry, Proto-Oncogene Proteins c-bcl-2 analysis, Proto-Oncogene Proteins c-bcl-2 metabolism, Reactive Oxygen Species analysis, Reactive Oxygen Species metabolism, Tumor Suppressor Protein p53 analysis, Tumor Suppressor Protein p53 metabolism, Zinc Compounds chemistry, bcl-2-Associated X Protein analysis, bcl-2-Associated X Protein metabolism, Antineoplastic Agents pharmacology, Apoptosis drug effects, Ferric Compounds pharmacology, Metal Nanoparticles chemistry, Nickel pharmacology, Zinc Compounds pharmacology
- Abstract
The long-term objective of the present study was to determine the ability of NiZn ferrite nanoparticles to kill cancer cells. NiZn ferrite nanoparticle suspensions were found to have an average hydrodynamic diameter, polydispersity index, and zeta potential of 254.2 ± 29.8 nm, 0.524 ± 0.013, and -60 ± 14 mV, respectively. We showed that NiZn ferrite nanoparticles had selective toxicity towards MCF-7, HepG2, and HT29 cells, with a lesser effect on normal MCF 10A cells. The quantity of Bcl-2, Bax, p53, and cytochrome C in the cell lines mentioned above was determined by colorimetric methods in order to clarify the mechanism of action of NiZn ferrite nanoparticles in the killing of cancer cells. Our results indicate that NiZn ferrite nanoparticles promote apoptosis in cancer cells via caspase-3 and caspase-9, downregulation of Bcl-2, and upregulation of Bax and p53, with cytochrome C translocation. There was a concomitant collapse of the mitochondrial membrane potential in these cancer cells when treated with NiZn ferrite nanoparticles. This study shows that NiZn ferrite nanoparticles induce glutathione depletion in cancer cells, which results in increased production of reactive oxygen species and eventually, death of cancer cells.
- Published
- 2013
- Full Text
- View/download PDF
28. Cytotoxicity of nickel zinc ferrite nanoparticles on cancer cells of epithelial origin.
- Author
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Al-Qubaisi MS, Rasedee A, Flaifel MH, Ahmad SH, Hussein-Al-Ali S, Hussein MZ, Eid EE, Zainal Z, Saeed M, Ilowefah M, Fakurazi S, Mohd Isa N, and El Zowalaty ME
- Subjects
- Antineoplastic Agents chemistry, Caspase 3 metabolism, Caspase 9 metabolism, Cell Line, Tumor, Cell Proliferation drug effects, Cell Shape drug effects, DNA Fragmentation drug effects, Ferric Compounds chemistry, Humans, Nickel chemistry, Particle Size, Spectrophotometry, Infrared, Statistics, Nonparametric, X-Ray Diffraction, Zinc Compounds chemistry, Antineoplastic Agents pharmacology, Cell Survival drug effects, Drug Carriers chemistry, Ferric Compounds pharmacology, Metal Nanoparticles chemistry, Nickel pharmacology, Zinc Compounds pharmacology
- Abstract
In this study, in vitro cytotoxicity of nickel zinc (NiZn) ferrite nanoparticles against human colon cancer HT29, breast cancer MCF7, and liver cancer HepG2 cells was examined. The morphology, homogeneity, and elemental composition of NiZn ferrite nanoparticles were investigated by scanning electron microscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, respectively. The exposure of cancer cells to NiZn ferrite nanoparticles (15.6-1,000 μg/mL; 72 hours) has resulted in a dose-dependent inhibition of cell growth determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The quantification of caspase-3 and -9 activities and DNA fragmentation to assess the cell death pathway of the treated cells showed that both were stimulated when exposed to NiZn ferrite nanoparticles. Light microscopy examination of the cells exposed to NiZn ferrite nanoparticles demonstrated significant changes in cellular morphology. The HepG2 cells were most prone to apoptosis among the three cells lines examined, as the result of treatment with NiZn nanoparticles. In conclusion, NiZn ferrite nanoparticles are suggested to have potential cytotoxicity against cancer cells.
- Published
- 2013
- Full Text
- View/download PDF
29. Thymoquinone-loaded nanostructured lipid carriers: preparation, gastroprotection, in vitro toxicity, and pharmacokinetic properties after extravascular administration.
- Author
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Abdelwahab SI, Sheikh BY, Taha MM, How CW, Abdullah R, Yagoub U, El-Sunousi R, and Eid EE
- Subjects
- Animals, Benzoquinones pharmacokinetics, Benzoquinones pharmacology, Benzoquinones therapeutic use, Cell Line, Cell Survival drug effects, Drug Carriers pharmacokinetics, Drug Carriers pharmacology, Histocytochemistry, Humans, Male, Nanoparticles therapeutic use, Nigella sativa, Particle Size, Protective Agents pharmacokinetics, Protective Agents pharmacology, Protective Agents therapeutic use, Rabbits, Rats, Rats, Sprague-Dawley, Stomach drug effects, Stomach pathology, Stomach Ulcer drug therapy, Stomach Ulcer pathology, Benzoquinones chemistry, Drug Carriers chemistry, Nanoparticles chemistry, Protective Agents chemistry
- Abstract
Background: Nanostructured lipid carriers (NLCs), composed of solid and liquid lipids, and surfactants are potentially good colloidal drug carriers. Thymoquinone is the main bioactive compound of Nigella sativa. In this study, the preparation, gastroprotective effects, and pharmacokinetic (PK) properties of thymoquinone (TQ)-loaded NLCs (TQNLCs) were evaluated., Method: TQNLCs were prepared using hydrogenated palm oil (Softisan® 154), olive oil, and phosphatidylcholine for the lipid phase and sorbitol, polysorbate 80, thimerosal, and double distilled water for the liquid lipid material. A morphological assessment of TQNLCs was performed using various methods. Analysis of the ulcer index, hydrogen concentration, mucus content, and biochemical and histochemical studies confirmed that the loading of TQ into the NLCs significantly improved the gastroprotective activity of this natural compound against the formation of ethanol-induced ulcers. The safety of TQNLC was tested on WRL68 liver normal cells with cisplatin as a positive control., Results: The average diameter of the TQNLCs was 75 ± 2.4 nm. The particles had negative zeta potential values of -31 ± 0.1 mV and a single melting peak of 55.85°C. Immunohistochemical methods revealed that TQNLCs inhibited the formation of ethanol-induced ulcers through the modulation of heat shock protein-70 (Hsp70). Acute hepatotoxic effects of the TQNLCs were not observed in rats or normal human liver cells (WRL-68). After validation, PK studies in rabbits showed that the PK properties of TQ were improved and indicated that the drug behaves linearly. The Tmax, Cmax, and elimination half-life of TQ were found to be 3.96 ± 0.19 hours, 4811.33 ± 55.52 ng/mL, and 4.4933 ± 0.015 hours, respectively, indicating that TQ is suitable for extravascular administration., Conclusion: NLCs could be a promising vehicle for the oral delivery of TQ and improve its gastroprotective properties.
- Published
- 2013
- Full Text
- View/download PDF
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