1. A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch
- Author
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Bruno Favery, Seyed Mahmoud Tabib Ghaffary, Cyrille Saintenac, Lamia Aouini, Julien Alassimone, Gert H. J. Kema, Oliver Robert, Andrea Sánchez-Vallet, Florence Cambon, Justin D. Faris, Thierry Langin, Théo Poucet, Hélène Berges, E.C.P. Verstappen, Maëlle Jaouannet, William Marande, Steven S. Xu, Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Wageningen University and Research [Wageningen] (WUR), Department of agronomy and Earth and Atmospheric Sciences [West Lafayette], Purdue University [West Lafayette], Seed and Plant Improvement Research Department, Agricultural Research, Education and Extension Organisation (AREEO ), Department of Plant Biology and Ecology (Bilbao, Spain), Universidad del Pais Vasco / Euskal Herriko Unibertsitatea [Espagne] (UPV/EHU), Université de Bordeaux Ségalen [Bordeaux 2], Centre National de Ressources Génomiques Végétales (CNRGV), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), 1.Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A., Cereal Crops Research Unit, USDA-ARS : Agricultural Research Service, Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Institute of Biochemistry [ETH Zürich], Department of Biology [ETH Zürich] (D-BIOL), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich)- Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Centro de Biotecnologia y Genomica de Plantas (CBGP), Laboratory of Phytopathology, Florimond Desprez, ETH Zurich Career Seed Grant (SEED-58 16-1)Fond de Soutien à l’Obtention Végétale, ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), Université Nice Sophia Antipolis (... - 2019) (UNS), Institut National de la Recherche Agronomique (France), ETH Zurich, Saintenac, Cyrille [0000-0002-3791-301X], Aouini, Lamia [0000-0001-6605-3488], Ghaffary, Seyed Mahmoud Tabib [0000-0001-7372-4163], Poucet, Théo [0000-0001-8910-9245], Berges, Hélène [0000-0002-5492-1062], Jaouannet, Maëlle [0000-0001-6635-5363], Favery, Bruno [0000-0003-3323-1852], Alassimone, Julien [0000-0002-8118-2605], Sánchez-Vallet, Andrea [0000-0002-3668-9503], Faris, Justin [0000-0002-9653-3287], Kema, Gert [0000-0002-2732-6911], Langin, Thierry [0000-0003-4955-4277], Saintenac, Cyrille, Aouini, Lamia, Ghaffary, Seyed Mahmoud Tabib, Poucet, Théo, Berges, Hélène, Jaouannet, Maëlle, Favery, Bruno, Alassimone, Julien, Sánchez-Vallet, Andrea, Faris, Justin, Kema, Gert, and Langin, Thierry
- Subjects
0106 biological sciences ,0301 basic medicine ,Unclassified drug ,[SDV]Life Sciences [q-bio] ,General Physics and Astronomy ,secreted protein ,avirulence ,01 natural sciences ,Plant breeding ,Septoria ,Pathogen ,Triticum ,Disease Resistance ,Plant Proteins ,aegilops-tauschii ,2. Zero hunger ,Genetics ,region ,Multidisciplinary ,Phosphotransferase ,1. No poverty ,food and beverages ,Complementary DNA ,Plants, Genetically Modified ,3. Good health ,Complementation ,rotein serine threonine kinase ,Seeds ,reveals ,Crops, Agricultural ,Science ,Mutagenesis (molecular biology technique) ,Virulence ,Protein Serine-Threonine Kinases ,Plant disease resistance ,Biology ,Genes, Plant ,Article ,General Biochemistry, Genetics and Molecular Biology ,Plant protein ,Biointeractions and Plant Health ,03 medical and health sciences ,Plant immunity ,Ascomycota ,zymoseptoria-tritici ,cultivars ,Life Science ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Cysteine ,genome ,Gene ,Cysteine rich receptor like kinase ,Alleles ,Comparative genomics ,disease ,Cell Membrane ,General Chemistry ,biology.organism_classification ,Laboratorium voor Phytopathologie ,030104 developmental biology ,Laboratory of Phytopathology ,identification ,EPS ,010606 plant biology & botany - Abstract
10 Pág. Centro de Biotecnología y Genómica de Plantas (CBGP), The poverty of disease resistance gene reservoirs limits the breeding of crops for durable resistance against evolutionary dynamic pathogens. Zymoseptoria tritici which causes Septoria tritici blotch (STB), represents one of the most genetically diverse and devastating wheat pathogens worldwide. No fully virulent Z. tritici isolates against synthetic wheats carrying the major resistant gene Stb16q have been identified. Here, we use comparative genomics, mutagenesis and complementation to identify Stb16q, which confers broad-spectrum resistance against Z. tritici. The Stb16q gene encodes a plasma membrane cysteine-rich receptor-like kinase that was recently introduced into cultivated wheat and which considerably slows penetration and intercellular growth of the pathogen., Our works was supported by the “Fond de Soutien à l’Obtention Vegetale” (FSOV), the French National Institute for Agricultural Research (INRA), and the ETH Zurich Career Seed Grant (SEED-58 16-1). We thank Sreedhar Kilaru and Gero Steinberg for providing the strain 3D7 expressing eGFP. Confocal laser scanning microscopy experiments were performed in the Scientific Center for Optical and Electron Microscopy (ScopeM), ETH Zurich and at the SPIBOC imaging facility of the Institut Sophia Agrobiotech. B.F. was supported by INRA and the French Government (National Research Agency, ANR) through the ‘Investments for the Future’ LabEx SIGNALIFE: program reference #ANR-11-LABX-0028-01.
- Published
- 2021
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