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1. Genomic analysis of the international high-risk clonal lineage Klebsiella pneumoniae sequence type 395

Author

Shaidullina, Elvira R., Schwabe, Michael, Rohde, Thomas, Shapovalova, Valeria V., Dyachkova, Marina S., Matsvay, Alina D., Savochkina, Yuliya A., Shelenkov, Andrey A., Mikhaylova, Yulia V., Sydow, Katharina, Lebreton, François, Idelevich, Evgeny A., Heiden, Stefan E., Becker, Karsten, Kozlov, Roman S., Shipulin, German A., Akimkin, Vasiliy G., Lalk, Michael, Guenther, Sebastian, Zautner, Andreas E., Bohnert, Jürgen A., Mardanova, Ayslu M., Bouganim, Ruth, Marchaim, Dror, Hoff, Katharina J., Schaufler, Katharina, and Edelstein, Mikhail V.

Published
2023
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5. Acinetobacter Non-baumannii Species: Occurrence in Infections in Hospitalized Patients, Identification, and Antibiotic Resistance

Author

Sheck, Eugene, primary, Romanov, Andrey, additional, Shapovalova, Valeria, additional, Shaidullina, Elvira, additional, Martinovich, Alexey, additional, Ivanchik, Natali, additional, Mikotina, Anna, additional, Skleenova, Elena, additional, Oloviannikov, Vladimir, additional, Azizov, Ilya, additional, Vityazeva, Vera, additional, Lavrinenko, Alyona, additional, Kozlov, Roman, additional, and Edelstein, Mikhail, additional

Published
2023
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6. Additional file 4 of Genomic analysis of the international high-risk clonal lineage Klebsiella pneumoniae sequence type 395

Author

Shaidullina, Elvira R., Schwabe, Michael, Rohde, Thomas, Shapovalova, Valeria V., Dyachkova, Marina S., Matsvay, Alina D., Savochkina, Yuliya A., Shelenkov, Andrey A., Mikhaylova, Yulia V., Sydow, Katharina, Lebreton, François, Idelevich, Evgeny A., Heiden, Stefan E., Becker, Karsten, Kozlov, Roman S., Shipulin, German A., Akimkin, Vasiliy G., Lalk, Michael, Guenther, Sebastian, Zautner, Andreas E., Bohnert, Jürgen A., Mardanova, Ayslu M., Bouganim, Ruth, Marchaim, Dror, Hoff, Katharina J., Schaufler, Katharina, and Edelstein, Mikhail V.

Abstract

Additional file 4: Identification of large recombination events and putative donors. Table S4. Large (>100 kbp) chromosome recombination events predicted by Gubbins and the putative donors identified by core-SNP phylogenetic analysis from the expanded genome dataset. Fig. S1. An example tree (A) and subtree (B) used to identify the ST437 as a putative donor for recombination event 13 in ST395 subclade D2.

Published
2023
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7. Additional file 2 of Genomic analysis of the international high-risk clonal lineage Klebsiella pneumoniae sequence type 395

Author

Shaidullina, Elvira R., Schwabe, Michael, Rohde, Thomas, Shapovalova, Valeria V., Dyachkova, Marina S., Matsvay, Alina D., Savochkina, Yuliya A., Shelenkov, Andrey A., Mikhaylova, Yulia V., Sydow, Katharina, Lebreton, François, Idelevich, Evgeny A., Heiden, Stefan E., Becker, Karsten, Kozlov, Roman S., Shipulin, German A., Akimkin, Vasiliy G., Lalk, Michael, Guenther, Sebastian, Zautner, Andreas E., Bohnert, Jürgen A., Mardanova, Ayslu M., Bouganim, Ruth, Marchaim, Dror, Hoff, Katharina J., Schaufler, Katharina, and Edelstein, Mikhail V.

Abstract

Additional file 2. Calculation of mean recombination counts. Formula for calculation of mean recombination counts.

Published
2023
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8. Additional file 3 of Genomic analysis of the international high-risk clonal lineage Klebsiella pneumoniae sequence type 395

Author

Shaidullina, Elvira R., Schwabe, Michael, Rohde, Thomas, Shapovalova, Valeria V., Dyachkova, Marina S., Matsvay, Alina D., Savochkina, Yuliya A., Shelenkov, Andrey A., Mikhaylova, Yulia V., Sydow, Katharina, Lebreton, François, Idelevich, Evgeny A., Heiden, Stefan E., Becker, Karsten, Kozlov, Roman S., Shipulin, German A., Akimkin, Vasiliy G., Lalk, Michael, Guenther, Sebastian, Zautner, Andreas E., Bohnert, Jürgen A., Mardanova, Ayslu M., Bouganim, Ruth, Marchaim, Dror, Hoff, Katharina J., Schaufler, Katharina, and Edelstein, Mikhail V.

Abstract

Additional file 3. Detailed Methods. Overview of used bioinformatics tools and parameters.

Published
2023
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9. Detection of carbapenemase-producing Enterobacterales by means of matrix-assisted laser desorption ionization time-of-flight mass spectrometry with ertapenem susceptibility-testing disks as source of carbapenem substrate

Author

Shaidullina, Elvira R., Romanov, Andrey V., Skleenova, Elena Y., Sheck, Eugene A., Sukhorukova, Marina V., Kozlov, Roman S., and Edelstein, Mikhail V.

Subjects
Microbiology (medical), Microbiology
Abstract

MALDI-TOF mass spectrometry has become widely used in clinical microbiology and has proved highly accurate for detection of carbapenemases in Gram-negative bacteria. However, the use of carbapenem-hydrolysis assays in routine diagnostics is hampered by the need for antibiotic substances and for making their fresh solutions each time an assay is conducted. Here, we evaluated the use of commercial antibiotic susceptibility-testing disks as source of ertapenem substrate in MALDI-TOF MS-based assay for detection of carbapenemase-producing Enterobacterales (CPE). The assay was validated on 48 CPE isolates of 8 different species expressing NDM-, VIM-, KPC- and OXA-48-type carbapenemases and exhibiting various levels of resistance to carbapenems (MIC range: 0.25– > 32 mg/l), as well as on 48 carbapenemase-non-producing isolates. The assay conditions were optimized as follows: 10-μl loopful of bacterial colonies was suspended in 150 μl 0.01 M Na-PBS buffer, pH 7.4, a 10 μg ertapenem susceptibility-testing disk was immersed in the suspension and incubated 3 h at 35°C, after which supernatant was obtained by centrifugation and applied on a target plate with alpha-cyano-4-hydroxycinnamic acid matrix. Mass spectra were analyzed between 440 and 560 m/z. Carbapenemase activity was detected in all tested CPE isolates by the appearance of m/z peaks corresponding to ertapenem hydrolysis products: [Mh + H]+:494.2, [Mh + Na]+:516.2, [Mh + 2Na]+:538.2, [Mh/d + H]+:450.2, [Mh/d + Na]+:472.2, and simultaneous decrease or loss of peaks of intact antibiotic: [M + H]+:476.2, [M + Na]+:498.1, [M + 2Na]+:520.1. No hydrolysis peaks or loss of intact ertapenem peaks were observed for carbapenemase-negative strains. We therefore report the development of a sensitive, specific and cost-effective MALDI-TOF MS-based assay for detection of CPE, which makes use of antibiotic disks readily available in most laboratories.

Published
2022

10. Molecular Epidemiology of mcr-1-Positive Escherichia coli and Klebsiella pneumoniae Isolates: Results from Russian Sentinel Surveillance (2013–2018)

Author

Shapovalova, Valeria, primary, Shaidullina, Elvira, additional, Azizov, Ilya, additional, Sheck, Eugene, additional, Martinovich, Alexey, additional, Dyachkova, Marina, additional, Matsvay, Alina, additional, Savochkina, Yulia, additional, Khafizov, Kamil, additional, Kozlov, Roman, additional, Shipulin, German, additional, and Edelstein, Mikhail, additional

Published
2022
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17. In vitro activity of biapenem and other carbapenems against Russian clinical isolates of Pseudomonas aeruginosa, Acinetobacter spp., and Enterobacterales

Author

Kozlov, Roman S., primary, Azyzov, Ilya S., additional, Dekhnich, Andrey V., additional, Ivanchik, Nataly V., additional, Kuzmenkov, Alexey Yu., additional, Martinovich, Alexey А., additional, Mikotina, Anna V., additional, Sukhorukova, Marina V., additional, Trushin, Ivan V., additional, and Edelstein, Mikhail V., additional

Published
2021
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18. Official statements of the board of experts on the use of topical thiamphenicol in patients with communityacquired respiratory infections

Author

Avdeev, Sergey N., primary, Garashchenko, T.I., additional, Geppe, N.A., additional, Dronov, I.A., additional, Zaitsev, Andrey A., additional, Ivanchik, Nataly V., additional, Ilenko, L.I., additional, Karpova, E.P., additional, Kozlov, Roman S., additional, Kozlova, Ludmila V., additional, Kolosova, N.G., additional, Malahov, A.B., additional, Nikiforova, G.N, additional, Odinaeva, N.D., additional, Svistushkin, V.M., additional, and Edelstein, Mikhail V., additional

Published
2021
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19. Susceptibility of clinical Enterobacterales and Pseudomonas aeruginosa isolates to ceftazidimeavibactam in Russia: multicenter local laboratory databased surveillance

Author

Edelstein, Mikhail V., primary, Skleenova, Elena Yu., additional, Trushin, Ivan V., additional, Kuzmenkov, Alexey Yu., additional, Martinovich, Alexey А., additional, Shek, Evgenij A., additional, Shajdullina, Elvira R., additional, Avramenko, Andrey A., additional, Vinogradova, Alina G., additional, Ivanchik, Nataly V., additional, Sukhorukova, Marina V., additional, Romanov, Andrey V., additional, Mikotina, Anna V., additional, Azyzov, Ilya S., additional, Dekhnich, Andrey V., additional, and Kozlov, Roman S., additional

Published
2021
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20. Antimicrobial Resistance and Genomic Characterization of OXA-48- and CTX-M-15-Co-Producing Hypervirulent Klebsiella pneumoniae ST23 Recovered from Nosocomial Outbreak

Author

Shaidullina, Elvira, primary, Shelenkov, Andrey, additional, Yanushevich, Yuri, additional, Mikhaylova, Yulia, additional, Shagin, Dmitriy, additional, Alexandrova, Irina, additional, Ershova, Olga, additional, Akimkin, Vasiliy, additional, Kozlov, Roman, additional, and Edelstein, Mikhail, additional

Published
2020
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24. Epidemiology and Genetic Diversity of Colistin Nonsusceptible Nosocomial Acinetobacter baumannii Strains from Russia for 2013-2014

Author

Sheck, Eugene A., Edelstein, Mikhail V., Sukhorukova, Marina V., Ivanchik, Natali V., Skleenova, Elena Yu., Dekhnich, Andrey V., Azizov, Ilya S., and Kozlov, Roman S.

Subjects
0301 basic medicine, Microbiology (medical), medicine.medical_specialty, Article Subject, 030106 microbiology, Infectious and parasitic diseases, RC109-216, Microbiology, 03 medical and health sciences, Antibiotic resistance, Epidemiology, Genotype, polycyclic compounds, medicine, Genetic diversity, biology, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification, QR1-502, Acinetobacter baumannii, Infectious Diseases, Colistin, bacteria, Research Article, medicine.drug
Abstract

A high level of resistance to carbapenems in Acinetobacter baumannii strains severely limits therapeutic possibilities. Colistin is the last resort drug against such strains, although the cases of resistance to this drug have become more frequent. This article presents the epidemiological features and genetic diversity of colistin nonsusceptible A. baumannii strains collected as part of a national multicenter epidemiological study of the antibiotic resistance of pathogens of nosocomial infections (MARATHON), which was conducted in 2013-2014 in Russia. A total of 527 A. baumannii isolates were collected, 10 (1.9%) of which were nonsusceptible to colistin. The majority of nonsusceptible A. baumannii isolates to colistin showed resistance to carbapenems and had the genes of the acquired OXA-40-like carbapenemases (n=6). In one case, a combination of OXA-23-like + OXA-40-like (n=1) genes was identified. One strain had the multidrug-resistant (MDR) phenotype, 6 isolates had extensively drug-resistant (XDR) phenotype, and 3 isolates had pandrug-resistant (PDR) phenotype. Among the colistin nonsusceptible A. baumannii isolates, 6 individual genotypes were identified, most of which belonged to successful international clones (CC92OXF/CC2PAS, n=4; CC944OXF/ST78PAS, n=4; CC109OXF/CC1PAS, n=1).

Published
2017

27. Novel Fri1-like Viruses Infecting Acinetobacter baumannii—vB_AbaP_AS11 and vB_AbaP_AS12—Characterization, Comparative Genomic Analysis, and Host-Recognition Strategy.

Author

Popova, Anastasia, primary, Lavysh, Daria, additional, Klimuk, Evgeniy, additional, Edelstein, Mikhail, additional, Bogun, Alexander, additional, Shneider, Mikhail, additional, Goncharov, Artemiy, additional, Leonov, Sergey, additional, and Severinov, Konstantin, additional

Published
2017
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31. In VitroResistance Studies with Bacteria That Exhibit Low Mutation Frequencies: Prediction of “Antimutant” Linezolid Concentrations Using a Mixed Inoculum Containing both Susceptible and Resistant Staphylococcus aureus

Author

Firsov, Alexander A., primary, Golikova, Maria V., additional, Strukova, Elena N., additional, Portnoy, Yury A., additional, Romanov, Andrey V., additional, Edelstein, Mikhail V., additional, and Zinner, Stephen H., additional

Published
2014
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32. Metallo-beta-lactamases as emerging resistance determinants in Gram-negative pathogens: open issues

Author

Cornaglia, Giuseppe Akova, Murat Amicosante, Gianfranco and Canton, Rafael Cauda, Roberto Docquier, Jean-Denis and Edelstein, Mikhail Frere, Jean-Marie Fuzi, Miklos Galleni, Moreno Giamarellou, Helen Gniadkowski, Marek Koncan, Raffaella Libisch, Balazs Luzzaro, Francesco Miriagou, Vivi and Navarro, Ferran Nordmann, Patrice Pagani, Laura Peixe, Luisa Poirel, Laurent Souli, Maria Tacconelli, Evelina and Vatopoulos, Alkiviadis Rossolini, Gian Maria ESGARS Grp

Abstract

The rapid spread of acquired metallo-beta-lactamases (MBLs) among major Gram-negative pathogens is a matter of particular concern worldwide and primarily in Europe, one of first continents where the emergence of acquired MBLs has been reported and possibly the geographical area where the increasing diversity of these enzymes and the number of bacterial species affected are most impressive. This spread has not been paralleled by accuracy/standardisation of detection methods, completeness of epidemiological knowledge or a clear understanding of what MBL production entails in terms of clinical impact, hospital infection control and antimicrobial chemotherapy. A number of European experts in the field met to review the current knowledge on this phenomenon, to point out open issues and to reinforce and relate to one another the existing activities set forth by research institutes, scientific societies and European Union-driven networks. (c) 2006 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

Published
2007

35. Bacterial Resistance Studies Using In Vitro Dynamic Models: the Predictive Power of the Mutant Prevention and Minimum Inhibitory Antibiotic Concentrations

Author

Firsov, Alexander A., primary, Strukova, Elena N., additional, Shlykova, Darya S., additional, Portnoy, Yury A., additional, Kozyreva, Varvara K., additional, Edelstein, Mikhail V., additional, Dovzhenko, Svetlana A., additional, Kobrin, Mikhail B., additional, and Zinner, Stephen H., additional

Published
2013
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37. Metallo-β-lactamases as emerging resistance determinants in Gram-negative pathogens: open issues

Author

Cornaglia, Giuseppe, primary, Akova, Murat, additional, Amicosante, Gianfranco, additional, Cantón, Rafael, additional, Cauda, Roberto, additional, Docquier, Jean-Denis, additional, Edelstein, Mikhail, additional, Frère, Jean-Marie, additional, Fuzi, Miklós, additional, Galleni, Moreno, additional, Giamarellou, Helen, additional, Gniadkowski, Marek, additional, Koncan, Raffaella, additional, Libisch, Balázs, additional, Luzzaro, Francesco, additional, Miriagou, Vivi, additional, Navarro, Ferran, additional, Nordmann, Patrice, additional, Pagani, Laura, additional, Peixe, Luisa, additional, Poirel, Laurent, additional, Souli, Maria, additional, Tacconelli, Evelina, additional, Vatopoulos, Alkiviadis, additional, and Rossolini, Gian Maria, additional

Published
2007
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38. Searching for the Optimal Predictor of Ciprofloxacin Resistance in Klebsiella pneumoniaeby Using In VitroDynamic Models

Author

Strukova, Elena N., Portnoy, Yury A., Romanov, Andrey V., Edelstein, Mikhail V., Zinner, Stephen H., and Firsov, Alexander A.

Abstract

ABSTRACTThere is growing evidence of applicability of the hypothesis of the mutant selection window (MSW), i.e., the range between the MIC and the mutant prevention concentration (MPC), within which the enrichment of resistant mutants is most probable. However, it is not clear if MPC-based pharmacokinetic variables are preferable to the respective MIC-based variables as interstrain predictors of resistance. To examine the predictive power of the ratios of the area under the curve (AUC24) to the MPC and to the MIC, the selection of ciprofloxacin-resistant mutants of three Klebsiella pneumoniaestrains with different MPC/MIC ratios was studied. Each organism was exposed to twice-daily ciprofloxacin for 3 days at AUC24/MIC ratios that provide peak antibiotic concentrations close to the MIC, between the MIC and the MPC, and above the MPC. Resistant K. pneumoniaemutants were intensively enriched at an AUC24/MIC ratio of 60 to 360 h (AUC24/MPC ratio from 2.5 to 15 h) but not at the lower or higher AUC24/MIC and AUC24/MPC ratios, in accordance with the MSW hypothesis. AUC24/MPC and AUC24/MIC relationships with areas under the time courses of ciprofloxacin-resistant K. pneumoniae(AUBCM) were bell shaped. These relationships predict highly variable “antimutant” AUC24/MPC ratios (20 to 290 h) compared to AUC24/MIC ratios (1,310 to 2,610 h). These findings suggest that the potential of the AUC24/MPC ratio as an interstrain predictor of K. pneumoniaeresistance is lower than that of the AUC24/MIC ratio.

Published
2016
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40. Long-Term Dissemination of CTX-M-5-Producing Hypermutable Salmonella entericaSerovar Typhimurium Sequence Type 328 Strains in Russia, Belarus, and Kazakhstan

Author

Kozyreva, Varvara K., Ilina, Elena N., Malakhova, Maja V., Carattoli, Alessandra, Azizov, Ilya S., Tapalski, Dmitry V., Kozlov, Roman S., and Edelstein, Mikhail V.

Abstract

ABSTRACTIn this paper, we present evidence of long-term circulation of cefotaxime-resistant clonally related Salmonella entericaserovar Typhimurium strains over a broad geographic area. The genetic relatedness of 88 isolates collected from multiple outbreaks and sporadic cases of nosocomial salmonellosis in various parts of Russia, Belarus, and Kazakhstan from 1996 to 2009 was established by multilocus tandem-repeat analysis (MLVA) and multilocus sequence typing (MLST). The isolates belong to sequence type 328 (ST328) and produce CTX-M-5 β-lactamase, whose gene is carried by highly related non-self-conjugative but mobilizable plasmids. Resistance to nalidixic acid and low-level resistance to ciprofloxacin is present in 37 (42%) of the isolates and in all cases is determined by various single point mutations in the gyrAgene quinolone resistance-determining region (QRDR). Isolates of the described clonal group exhibit a hypermutable phenotype that probably facilitates independent acquisition of quinolone resistance mutations.

Published
2014
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41. Bacterial Resistance Studies Using In VitroDynamic Models: the Predictive Power of the Mutant Prevention and Minimum Inhibitory Antibiotic Concentrations

Author

Firsov, Alexander A., Strukova, Elena N., Shlykova, Darya S., Portnoy, Yury A., Kozyreva, Varvara K., Edelstein, Mikhail V., Dovzhenko, Svetlana A., Kobrin, Mikhail B., and Zinner, Stephen H.

Abstract

ABSTRACTIn light of the concept of the mutant selection window, i.e., the range between the MIC and the mutant prevention concentration (MPC), MPC-related pharmacokinetic indices should be more predictive of bacterial resistance than the respective MIC-related indices. However, experimental evidence of this hypothesis remains limited and contradictory. To examine the predictive power of the ratios of the area under the curve (AUC24) to the MPC and the MIC, the selection of ciprofloxacin-resistant mutants of four Escherichia colistrains with different MPC/MIC ratios was studied. Each organism was exposed to twice-daily ciprofloxacin for 3 days at AUC24/MIC ratios that provide peak antibiotic concentrations close to the MIC, between the MIC and the MPC, and above the MPC. Resistant E. coliwas intensively enriched at AUC24/MPCs from 1 to 10 h (AUC24/MIC from 60 to 360 h) but not at the lower or higher AUC24/MPC and AUC24/MIC ratios. AUC24/MPC and AUC24/MIC relationships of the areas under the time courses of ciprofloxacin-resistant E. coli(AUBCM) were bell-shaped. A Gaussian-like function fits the AUBCM-AUC24/MPC and AUBCM-AUC24/MIC data combined for all organisms (r2= 0.69 and 0.86, respectively). The predicted anti-mutant AUC24/MPC ratio was 58 ± 35 h, and the respective AUC24/MIC ratio was 1,080 ± 416 h. Although AUC24/MPC was less predictive of strain-independent E. coliresistance than AUC24/MIC, the established anti-mutant AUC24/MPC ratio was closer to values reported for Staphylococcus aureus(60 to 69 h) than the respective AUC24/MIC ratio (1,080 versus 200 to 240 h). This implies that AUC24/MPC might be a better interspecies predictor of bacterial resistance than AUC24/MIC.

Published
2013
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42. Detection of sequence variation in PCR‐amplified fragments of omp2gene from three species of the family Chlamydiaceae using agarose gel electrophoresis containing bisbenzimide‐PEG

Author

Demkin, Vladimir V., Edelstein, Mikhail V., Zimin, Andrey L., Edelstein, Inna A., and Suvorov, Maxim M.

Abstract

A simple technique providing a means for rapid genetic differentiation of chlamydial strains is described. The technique is based on a single‐step sequence‐specific separation of PCR‐amplified DNA fragments by electrophoresis in an agarose gel containing a DNA ligand – bisbenzimide‐PEG. A hypervariable region at the 5′ end of the omp2gene of Chlamydiaceae species encoding the 60‐kDa cysteine‐rich outer membrane protein was selected as a target for PCR. The appropriate fragments were amplified from strains of Chlamydia trachomatis, Chlamydophila pneumoniae, and Chlamydophila psittaci, and the PCR products originating from different species were electrophoretically separated in the presence of the DNA ligand. We therefore demonstrated that PCR with a single pair of primers followed by simple agarose gel electrophoresis with bisbenzimide‐PEG can be applied to the differentiation of three members of the family Chlamydiaceae which are commonly recognized as human pathogens.

Published
2000
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43. Abstracts from the 8th International Congress of the Asia Pacific Society of Infection Control (APSIC)

Author

Sutthiruk, Nantanit, Botti, Mari, Considine, Julie, Driscoll, Andrea, Hutchinson, Ana, Malathum, Kumthorn, Cucunawangsih, Cucunawangsih, Wiwing, Veronica, Puspitasari, Vivien, Shanmugakani, Rathina Kumar, Akeda, Yukihiro, Kodera, Takuya, Santanirand, Pitak, Tomono, Kazunori, Yamanaka, Takayuki, Moriuchi, Hiroyuki, Kitajima, Hiroyuki, Horikoshi, Yuho, Lavrinenko, Alyona, Azizov, Ilya, Tabriz, Nurlan, Kozhamuratov, Margulan, Serbo, Yekatherine, Yang, Dahae, Lee, Woonhyoung, Bae, Il Kwon, Lee, Jae Hyun, Lee, Hyukmin, Kim, Jung Ok, Jeong, Seok Hoon, Lee, Kyungwon, Peremalo, Thiba, Madhavan, Priya, Hamzah, Sharina, Than, Leslie, Wong, Eng Hwa, Desa, Mohd Nasir Mohd, Ng, Kee Peng, Geronimo, Marionne, Tayzon, Maria Fe, Maño, Maria Jesusa, Chow, Angela, Hon, Pei-Yun, Win, Mar-Kyaw, Ang, Brenda, Leo, Yee-Sin, See, Tina, Marin, Rocio Alvarez, de Sousa, Marta Aires, Kieffer, Nicolas, Nordmann, Patrice, Poirel, Laurent, Laochareonsuk, Wison, Petyu, Sireekul, Wanasitchaiwat, Pawin, Thana, Sutasinee, Bunyaphongphan, Chollathip, Boonsomsuk, Woranan, Maneepongpermpoon, Pakpoom, Jamulitrat, Silom, Sureshkumar, Dorairajan, Supraja, Kalyanaraman, Sharmila, Soundararajan, Setiawan, Benny, Lumbuun, Nicolaski, Nakayama, Haruo, Ota, Toshiko, Shirane, Naoko, Matuoka, Chikako, Kodama, Kentaro, Ohtsuka, Masanobu, Bacolcol, Silverose Ann Andales, Velmonte, Melecia, Alde, Allan, Chavez, Keithleen, Esteban, Arlene Joy, Lee, Aisa Jensen, Hsieh, Tai-Chin, Shio-ShinJean, Huang, Huey-Jen, Huang, Shu-Ju, Huang, Yu-Huan, Cheng, Pei-Chen, Yu, Su-Fang, Tsao, Shih-Ming, Lee, Yuan-Ti, Li, Chien-Feng, Lu, Min-Chi, Pruetpongpun, Nattapol, Khawcharoenporn, Thana, Damronglerd, Pansachee, Suwantarat, Nuntra, Apisarnthanarak, Anucha, Rutjanawech, Sasinuch, Cushinotto, Lisa, McBride, Patty, Williams, Harding, Liu, Hans, Hang, Phan Thi, Anh, Dinh Pham Phuong, Le, Ngai, Khu, Dung, Nguyen, Lam, Castillo, Roel Beltran, Gopalakrishnan, Ram, Ramasubramanian, Venkatasubramanian, Sreevidya, Subramanian, Jayapradha, Ranganathan, Umetsu, Atsushi, Noda, Tetsuhiro, Hashimoto, Kenyuu, Hayashi, Akihiro, Kabashima, Mikie, Jadczak, Ursula, Elvelund, Knut, Johnsen, Marit, Borgen, Bente, Lingaas, Egil, Mao, Chia-Hua, Chang, Fu-Chieh, Liu, Chang-Pan, Chao, Ru-Hui, Chang, Fu-chieh, Liu, Chang-pan, Pawapotako, Junpen, Prasertpan, Chadanan, Malaihuan, Wantanee, Uirungroj, Phisit, Saenjum, Chalermpong, Ouirungrog, Teerapat, Borrell, Sue, Bass, Pauline, Worth, Leon, Xian-li, Zhao, Xiao-long, Li, Xue-hua, Yao, Wei, Ren, Zeng, Zhang Xia, Kong, Man Ying, Lai, Christopher Koon Chi, Lee, Suet Yi, Tsang, Ngai Chong, O’Donoghue, M. M., Boost, M. V., Suen, L. K. P., Siu, G. K., Mui, K. W., Lai, C. K. C., Tsang, D. N. C., Sato, Yuka, Tateishi, Mariko, Mihashi, Mutsuko, Flor, Jose Paulo, Bautista, Marko, De Roxas, V. Jay, Vergara, Justine, Añonuevo, Nicolo Andrei, Kwek, Marion, Acuin, Jose, Sanchez, Anna Josea, Bathan, Avel, Jantan, Jamilah Binte, Guek, Chua Chor, Kian, Eu Chiow, Pirido, Pampe Anak, Aron, Nur Fadilah Binte Mohd, Estacio, Leah May, Palana, Francis Alvarez, Gracia, Michelle, Shamsuddin, Nur Syafiqah Binte, Castro, Kersten Timbad, Baloria, Madonna, Adam, Faezah Binte, Wei, Zhang, Fong, Poh Bee, Kalisvar, Marimuthu, Chuang, I-Ju, Yi-ChunCho, Chiu, Yu-Fen, Chen, Lung-Chih, Lin, Yi-Chun, Dong, Shao-Xing, Lee, Yi-Chieh, Kuan, Hui-Chen, Lin, Hsin-Hua, Chi, Chia-Chun, Lu, Chin-Te, Ya-Fen, Tang, Li-Hsiang, Su, Jien-Wei, Liu, Chao, Hsuehlan, ChangChien, PinRu, Chen, WeiFang, Lai, ChungHsu, Ara, Lutfe, Mowla, Syed Mohammad Niaz, Vashkar, Shaikh Mahmud Kamal, Chan, Wai Fong, ChunYau, Mabel Yin, LingChong, Karen Kam, OnLi, Tze, Kaur, Rajwinder, Yan, Ng Po, Chiu, Gloria Chor Shan, Cheung, Christina W. Y., Ching, Patricia T. Y., Ching, Radley H. C., Lam, Conita H. S., Kan, C. H., Lee, Shirley S. Y., Chen, C. P., Chan, Regina F. Y., Leung, Annie F. Y., Wong, Isadora L. C., Lam, S. S., Chan, Queenie W. L., Chan, Cecilia, Nematian, Seyed Sadeq Seyed, Palenik, Charles John, Askarian, Mehrdad, Hatam, Nahid, Nakamura, Itaru, Fujita, Hiroaki, Tsukimori, Ayaka, Kobayashi, Takehito, Sato, Akihiro, Fukushima, Shinji, Matsumoto, Tetsuya, De Roxas, V. James, De Roxas, V James, AndreiAñonuevo, Nicolo, Ho, Yeng May, Kum, Jia Qi, Poh, Bee Fong, Marimuthu, Kalisvar, Liu, Tzu-Yin, Chu, Sin-Man, Chen, Hui-Zhu, Chen, Tun-chieh, Chen, Yichun, Tsao, Ya-Ching, Skuntaniyom, Sumawadee, Tipluy, Pirawadee, Paengta, Sangwan, wongsaen, Ratchanee, thanomphan, Sutthiphun, Tariyo, Samettanet, Thongchuea, Buachan, Khamfu, Pattama, Thanomphan, Sutthiphan, Songtaweesin, Wipaporn Natalie, Anugulruengkit, Suvaporn, Samransamruajkit, Rujipat, Sosothikul, Darintr, Tansrijitdee, Ornanong, Nakphunsung, Anry, Srimuan, Patchareeyawan, Sophonphan, Jirachaya, ThanyaweePuthanakit, Payuk, Kunyanut, Picheansathian, Wilawan, Viseskul, Nongkran, DeNardo, Elizabeth, Leslie, Rachel, Cartner, Todd, Barbosa, Luciana, Werner, Heinz-Peter, Brill, Florian H. H., Kawagoe, Julia Yaeko, De Nardo, Elizabeth, Wilson, Sarah Edmonds, Macinga, David, Mays-Suko, Patricia, Duley, Collette, Hang, Tran Thi Thuy, Hanh, Tran Thi My, Gordon, Christopher, Durairaj, Roopa, Rohit, Anusha, Saravanakumar, Saujanya, Hemalatha, Jothymani, Hirano, Ryuichi, Sakamoto, Yuichi, Yamamoto, Shoji, Tachibana, Naoki, Miura, Miho, Hieda, Fumiyo, Sakai, Yoshiro, Watanabe, Hiroshi, Bacolcol, Silverose Ann, Chavez, Keitleen, Lim, Jia-Wei, Hein, Aung-Aung, Tin, Grace, Lim, Vanessa, Chao, Huwi-chun, Yeh, Chiu-Yin, Lo, Mei-feng, Piwpong, Chonlada, Rajborirug, Songyos, Preechawetchakul, Ploypailin, Pruekrattananapa, Yada, Sangsuwan, Tharntip, Wongsaen, Ratchanee, Paengta, Sungwan, Nilchon, Napatnun, Thanompan, Sutthipun, Tariyo, Samattanet, Kolesnichenko, Svetlana, Tishkambayev, Yerbol, Alibecov, Asylkhan, Serbo, Yekaterina, Nam, Youngwon, Park, Jae Hyeon, Hong, Yun Ji, Kim, Taek Soo, Park, Jeong Su, Park, Kyoung Un, Kim, Eui-Chong, Aziegbemhin, Samuel Abumhere, Enabulele, Onaiwu, Tung, Yao-Shen, Chen, An-Chi, Huang, Shen-Min, Yang, Yui-Yein, Wu, Li-Hung, Lin, Chin-cheng, Lien, Tzu Hao, Chang, Jia Hao, Huang, Yu Shan, Chen, Yi Shun, Sirilun, Sasithorn, Ouirungroj, Phisit, Trakulsomboon, Suwanna, Prasajak, Patcharee, Kwok, Maryanne W. N., Ng, Lady S. H., Wong, Lindy M. T., Poon, Lenina S. L., Lai, Mary K. L., Cheng, Holly H. S., Fong, S. K., Leung, Cindy F. Y., Hasegawa, Jumpei, Shirakawa, Hiroki, Wakai, Sachiko, Mieno, Makiko, Hatakeyama, Shuji, Deeudom, Manu, Tharavichitkul, Prasit, Chinniah, Terrence, Tan, Jackson, Prabu, Kavitha, Alam, Sartaj, Wynn, Aung Kyaw, Ahmad, Rashidah, Sidek, Amalina, Samsuddin, Dg Azizah, Ajis, Noraini, Ahmad, Aliyah, Magon, Susylawathi, Chu, Boon, Kuang, Jiqiu, Gao, Yan, Wang, Shoujun, Hao, Yunxiao, Liu, Rong, Li, Dongmei, Wang, Hui, Nishio, Hisanori, Mori, Hitomi, Morokuma, Yoshiko, Yamada, Takaaki, Kiyosuke, Makiko, Yasunaga, Sachie, Toyoda, Kazuhiro, Shimono, Nobuyuki, Babenko, Dmitriy, Turmuhambetova, Anar, Cheşcă, Antonella, Toleman, Mark A., Akhmaltdinova, Lyudmila L., Magsakay, Mark Albert, Macatibag, Angelo, Lerios, Jeannica Kriselle, Lavrineko, Alyona, Babenko, Dmitry, Sheck, Eugene, Edelstein, Mikhail, Li, Lih-Yue, Chan, Chiung-Wen, Pan, Hui-Chuan, Vanishakije, Wipa, Jaikampun, Warisra, Li, Su-Yin, Li, Jian-Feng, Wu, Yu-Ping, Lin, Chiao-Hui, Chang, Ping-Chin, Tariyo, Samatanet, Thanompan, Suttsiphan, Sukkra, Suchada, Zaman, Khalequ, Zaman, Sheikh Farzana, Zaman, Farzana, Aziz, Asma, Faisal, Sayeed-Bin, Traskine, Magali, Ruiz-Guiñazú, Javier, Borys, Dorota, Lam, Wendy Wai Yee, Chow, May, Choy, Lucy, Kam, Joseph, Salleh, Sharifah Azura, Yacob, Razila, Yusof, Siti Rokiah, Jalil, Nordiah Awang, Millan, Maria Lourdes, Acuin, Jose Lito, Velmonte, Melecia A., Bacolcol, Silverose Ann A., Ting, Ching-I, Dissayasriroj, Sunisa, Chinniah, Terrence Rohan, DiniSuhaimi, Jauharatud, Mirasin, Aizzuddin, Morni, Nurul, Samsuddin, Azizah, AbuBakar, Amalina, Shafiee, Amanie, Safar, Julaini, Annie, Leung, Ling, Fung Yuk, Edna, Lau, Kristine, Luk, Shinomiya, Satoshi, Yamamoto, Kumiko, Kjiwara, Kayoko, Yamaguchi, Mitsuhiro, Zhang, Wei, Poh, Bee-Fong, Chan, Ming-Chin, Wang, Chih-Chien, Huang, Huan-Yu, Lai, Chiung-Ling, Kosol, Sajeerat, Sakolwirat, Wantana, Paepong, Patchanee, Jansanga, Sawalee, Jaisamoot, Pattarin, Thongnuanual, Nuttha, Srithong, Chittima, Somsakul, Somporn, Plongpunth, Sutima, Punpop, Mukkapon, Malathum, Porntip, Peautiwat, Kulada, boon kirdram, Nattawipa, Klunklin, Pimpaporn, Samethadka, Geetha, Suzuki, Naoko, Asada, Hitomi, Katayama, Masao, Komano, Atsushi, Watanabe, Hidehiro, Seo, Hye Kyung, Hwang, Joo-Hee, Shin, Myoung Jin, Kim, Su Young, Kim, Eu Suk, Song, Kyoung-Ho, Kim, Hong Bin, Un, Lai-Si, Vong, Choi-Ian, Koh, Jocelyn, Agustinus, Sherly, Hassan, Rozita Bte Abu, Thinn, Yin Phyu, Ng, Benjamin, Tun, Soe Pyae, Ha, Su Mon Thi, Xiaoting, Xue, Li, Lin, Chuang, Leyland, Niroshika, Attanayaka Mudiyanselage Chulani, Perera, Kaluarachchige Anoma Kaluarachchi, Fernando, Dimingo Kankanamalage Diana Grace, Hemamala, Bodhipakshage Rohini, Yeh, Chiu-yin, Yang, Hui-Chun, Chiu, Hsiang-Ju, Shih, Ya-Ling, Chien, Yu-Shan, Lin, Wan-Yi, Pan, Chia-Yun, Chang, Ying-Yun, Yea, Chiu-Yuch, Chu, Ming-Hsien, Lee, Li-Chu, Yu-Hsiu, Lin, Siao-Pei, Guo, Pak-On, Leung, Mei-Fe, Sie, Jyh-Jou, Chen, Yong-Yuan, Chang, Kuo, Shu-Yuan, Lin, Yu-Hsiu, Zhang, Ji-Sheng, Leung, Pak-On, Sie, Mei-Fe, Chen, Jyh-Jou, Chen, Yan-Ru, Chen, Ying-Ling, Taou, Chi-Fen, Chen, Hsiao-Shan, Tang, Hung-Jen, Chen, Shin Yu, Chen, Yin Yin, Der Wang, Fu, Shih, Tzu-Ping, Chen, Chin-Yu, Chen, Su-Jung, Wu, Mei-chi, Yang, Wan-ju, Chou, Mei-ling, Yu, Man-Ling, Li, Li-Chu, Chu, Cheng-Wei, Tsou, Wen-Hao, Wu, Wen-Chih, Cheng, Wen-Chi, Sun, Cho-Ching, Lu, Shu-Hua, Yang, Hsin-Ling, Lu, Cheng-Yu, Hirunprapakorn, Nitchawan, Apivanich, Sirilux, Pornmee, Ttipakorn, Beowsomboon, Chonnikarnt, Kumkoom, Itthaporn, Kasatpibal, Nongyao, Chitreecheur, Jittaporn, Whitney, JoAnne D., Saokaew, Surasak, Kengkla, Kirati, Heitkemper, Margaret M., Muntajit, Thanomvong, Apivanich, Siriluk, Phan, Hang Thi, Dinh, Anh Pham Phuong, and Nguyen, Tuyet Thi Kim

Subjects
lcsh:RC109-216, Meeting Abstracts, lcsh:Infectious and parasitic diseases
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44. Epidemiology and Genetic Diversity of Colistin Nonsusceptible Nosocomial Acinetobacter baumannii Strains from Russia for 2013-2014

Author

A. Sheck, Eugene, V. Edelstein, Mikhail, V. Sukhorukova, Marina, V. Ivanchik, Natali, Yu. Skleenova, Elena, V. Dekhnich, Andrey, S. Azizov, Ilya, and S. Kozlov, Roman

Abstract

A high level of resistance to carbapenems in Acinetobacter baumannii strains severely limits therapeutic possibilities. Colistin is the last resort drug against such strains, although the cases of resistance to this drug have become more frequent. This article presents the epidemiological features and genetic diversity of colistin nonsusceptible A. baumannii strains collected as part of a national multicenter epidemiological study of the antibiotic resistance of pathogens of nosocomial infections (MARATHON), which was conducted in 2013-2014 in Russia. A total of 527 A. baumannii isolates were collected, 10 (1.9%) of which were nonsusceptible to colistin. The majority of nonsusceptible A. baumannii isolates to colistin showed resistance to carbapenems and had the genes of the acquired OXA-40-like carbapenemases (n=6). In one case, a combination of OXA-23-like + OXA-40-like (n=1) genes was identified. One strain had the multidrug-resistant (MDR) phenotype, 6 isolates had extensively drug-resistant (XDR) phenotype, and 3 isolates had pandrug-resistant (PDR) phenotype. Among the colistin nonsusceptible A. baumannii isolates, 6 individual genotypes were identified, most of which belonged to successful international clones (CC92OXF/CC2PAS, n=4; CC944OXF/ST78PAS, n=4; CC109OXF/CC1PAS, n=1).

Published
2017
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45. Detection of sequence variation in PCR-amplified fragments of omp2 gene from three species of the family Chlamydiaceae using agarose gel electrophoresis containing bisbenzimide-PEG

Author

Demkin, Vladimir V., Edelstein, Mikhail V., Zimin, Andrey L., Edelstein, Inna A., and Suvorov, Maxim M.

Abstract

A simple technique providing a means for rapid genetic differentiation of chlamydial strains is described. The technique is based on a single-step sequence-specific separation of PCR-amplified DNA fragments by electrophoresis in an agarose gel containing a DNA ligand – bisbenzimide-PEG. A hypervariable region at the 5′ end of the omp2 gene of Chlamydiaceae species encoding the 60-kDa cysteine-rich outer membrane protein was selected as a target for PCR. The appropriate fragments were amplified from strains of Chlamydia trachomatis, Chlamydophila pneumoniae, and Chlamydophila psittaci, and the PCR products originating from different species were electrophoretically separated in the presence of the DNA ligand. We therefore demonstrated that PCR with a single pair of primers followed by simple agarose gel electrophoresis with bisbenzimide-PEG can be applied to the differentiation of three members of the family Chlamydiaceae which are commonly recognized as human pathogens.

Published
2000
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46. Searching for the Optimal Predictor of Ciprofloxacin Resistance in Klebsiella pneumoniae by Using In Vitro Dynamic Models.

Author

Strukova EN, Portnoy YA, Romanov AV, Edelstein MV, Zinner SH, and Firsov AA

Subjects
Anti-Bacterial Agents pharmacokinetics, Area Under Curve, Ciprofloxacin pharmacokinetics, Drug Resistance, Bacterial genetics, Klebsiella pneumoniae genetics, Microbial Sensitivity Tests, Models, Biological, Mutation, Anti-Bacterial Agents pharmacology, Ciprofloxacin pharmacology, Drug Resistance, Bacterial drug effects, Klebsiella pneumoniae drug effects
Abstract

There is growing evidence of applicability of the hypothesis of the mutant selection window (MSW), i.e., the range between the MIC and the mutant prevention concentration (MPC), within which the enrichment of resistant mutants is most probable. However, it is not clear if MPC-based pharmacokinetic variables are preferable to the respective MIC-based variables as interstrain predictors of resistance. To examine the predictive power of the ratios of the area under the curve (AUC24) to the MPC and to the MIC, the selection of ciprofloxacin-resistant mutants of three Klebsiella pneumoniae strains with different MPC/MIC ratios was studied. Each organism was exposed to twice-daily ciprofloxacin for 3 days at AUC24/MIC ratios that provide peak antibiotic concentrations close to the MIC, between the MIC and the MPC, and above the MPC. Resistant K. pneumoniae mutants were intensively enriched at an AUC24/MIC ratio of 60 to 360 h (AUC24/MPC ratio from 2.5 to 15 h) but not at the lower or higher AUC24/MIC and AUC24/MPC ratios, in accordance with the MSW hypothesis. AUC24/MPC and AUC24/MIC relationships with areas under the time courses of ciprofloxacin-resistant K. pneumoniae (AUBCM) were bell shaped. These relationships predict highly variable "antimutant" AUC24/MPC ratios (20 to 290 h) compared to AUC24/MIC ratios (1,310 to 2,610 h). These findings suggest that the potential of the AUC24/MPC ratio as an interstrain predictor of K. pneumoniae resistance is lower than that of the AUC24/MIC ratio., (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)

Published
2015
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47. In vitro resistance studies with bacteria that exhibit low mutation frequencies: prediction of "antimutant" linezolid concentrations using a mixed inoculum containing both susceptible and resistant Staphylococcus aureus.

Author

Firsov AA, Golikova MV, Strukova EN, Portnoy YA, Romanov AV, Edelstein MV, and Zinner SH

Subjects
Anti-Bacterial Agents pharmacology, Linezolid, Microbial Sensitivity Tests, Mutation Rate, Staphylococcus aureus drug effects, Acetamides pharmacology, Methicillin-Resistant Staphylococcus aureus drug effects, Oxazolidinones pharmacology
Abstract

Bacterial resistance studies using in vitro dynamic models are highly dependent on the starting inoculum that might or might not contain spontaneously resistant mutants (RMs). To delineate concentration-resistance relationships with linezolid-exposed Staphylococcus aureus, a mixed inoculum containing both susceptible cells and RMs was used. An RM selected after the 9th passage of the parent strain (MIC, 2 μg/ml) on antibiotic-containing media (RM9; MIC, 8 μg/ml) was chosen for the pharmacodynamic studies, because the mutant prevention concentration (MPC) of linezolid against the parent strain in the presence of RM9 at 10(2) (but not at 10(4)) CFU/ml did not differ from the MPC value determined in the absence of the RMs. Five-day treatments with twice-daily linezolid doses were simulated at concentrations either between the MIC and MPC or above the MPC. S. aureus RMs (resistant to 2× and 4×MIC but not 8× and 16×MIC) were enriched at ratios of the 24-h area under the concentration-time curve (AUC24) to the MIC that provide linezolid concentrations between the MIC and MPC for 100% (AUC24/MIC, 60 h) and 86% (AUC24/MIC, 120 h) of the dosing interval. No such enrichment occurred when linezolid concentrations were above the MIC and below the MPC for a shorter time (37% of the dosing interval; AUC24/MIC, 240 h) or when concentrations were consistently above the MPC (AUC24/MIC, 480 h). These findings obtained using linezolid-susceptible staphylococci supplemented with RMs support the mutant selection window hypothesis. This method provides an option to delineate antibiotic concentration-resistance relationships with bacteria that exhibit low mutation frequencies., (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)

Published
2015
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48. Convergent in vivo and in vitro selection of ceftazidime resistance mutations at position 167 of CTX-M-3 beta-lactamase in hypermutable Escherichia coli strains.

Author

Stepanova MN, Pimkin M, Nikulin AA, Kozyreva VK, Agapova ED, and Edelstein MV

Subjects
Directed Molecular Evolution, Escherichia coli enzymology, Escherichia coli genetics, Escherichia coli Proteins genetics, Escherichia coli Proteins metabolism, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Selection, Genetic, Sequence Analysis, DNA, beta-Lactamases chemistry, Anti-Bacterial Agents pharmacology, Ceftazidime pharmacology, Cephalosporin Resistance, Escherichia coli drug effects, Evolution, Molecular, Mutation, beta-Lactamases genetics
Abstract

We report on a novel CTX-M extended-spectrum beta-lactamase (ESBL), designated CTX-M-42, with enhanced activity toward ceftazidime. CTX-M-42 was identified in a hypermutable Escherichia coli nosocomial isolate (isolate Irk2320) and is a Pro167Thr amino acid substitution variant of CTX-M-3. By molecular typing of ESBL-producing E. coli strains previously isolated in the same hospital ward, we were able to identify a putative progenitor (strain Irk1224) of Irk2320, which had a mutator phenotype and harbored the CTX-M-3 beta-lactamase. To reproduce the natural evolution of CTX-M-3, we selected for ceftazidime resistance mutations in bla CTX-M-3 gene in vitro both in clinical isolate Irk1224 and in laboratory-derived hypermutable (mutD5) strain GM2995. These experiments yielded CTX-M-3 Pro167Ser and CTX-M-3 Asn136Lys mutants which conferred higher levels of resistance to ceftazidime than to cefotaxime. CTX-M-3 Asn136Lys had a level of low activity toward ampicillin, which may explain its absence from clinical isolates. We conclude that the selection of CTX-M-42 could have occurred in vivo following treatment with ceftazidime and was likely facilitated by the hypermutable background.

Published
2008
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49. Metallo-beta-lactamases as emerging resistance determinants in Gram-negative pathogens: open issues.

Author

Cornaglia G, Akova M, Amicosante G, Cantón R, Cauda R, Docquier JD, Edelstein M, Frère JM, Fuzi M, Galleni M, Giamarellou H, Gniadkowski M, Koncan R, Libisch B, Luzzaro F, Miriagou V, Navarro F, Nordmann P, Pagani L, Peixe L, Poirel L, Souli M, Tacconelli E, Vatopoulos A, and Rossolini GM

Subjects
Anti-Bacterial Agents therapeutic use, Drug Resistance, Multiple, Bacterial, European Union, Gram-Negative Bacteria classification, Gram-Negative Bacteria drug effects, Gram-Negative Bacterial Infections drug therapy, Gram-Negative Bacterial Infections epidemiology, Gram-Negative Bacterial Infections prevention & control, Humans, Microbial Sensitivity Tests, beta-Lactam Resistance, beta-Lactamases drug effects, Gram-Negative Bacteria isolation & purification, Gram-Negative Bacteria physiology, Gram-Negative Bacterial Infections microbiology, beta-Lactamases physiology
Abstract

The rapid spread of acquired metallo-beta-lactamases (MBLs) among major Gram-negative pathogens is a matter of particular concern worldwide and primarily in Europe, one of first continents where the emergence of acquired MBLs has been reported and possibly the geographical area where the increasing diversity of these enzymes and the number of bacterial species affected are most impressive. This spread has not been paralleled by accuracy/standardisation of detection methods, completeness of epidemiological knowledge or a clear understanding of what MBL production entails in terms of clinical impact, hospital infection control and antimicrobial chemotherapy. A number of European experts in the field met to review the current knowledge on this phenomenon, to point out open issues and to reinforce and relate to one another the existing activities set forth by research institutes, scientific societies and European Union-driven networks.

Published
2007
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