1. AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells
- Author
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Jiří Friml, David Domjan, Shutang Tan, Matyáš Fendrych, Satoshi Naramoto, Eike Hendrik Rademacher, Maritza van Dop, Xixi Zhang, Alexander W. Johnson, Matouš Glanc, Lukas Hoermayer, Robert Hauschild, Ludmila Včelařová, Xiaoyu Wei, Remko Offringa, Edward de Koning, Bert De Rybel, Stef Janson, Kasper van Gelderen, Gergely Molnár, Sub Plant Ecophysiology, and Plant Ecophysiology
- Subjects
0301 basic medicine ,POLARIZATION ,Polarity (physics) ,ENDOCYTOSIS ,Neuroscience(all) ,Mutant ,Arabidopsis ,DEPENDENT AUXIN TRANSPORT ,GRADIENTS ,urologic and male genital diseases ,Plant Roots ,Biochemistry ,General Biochemistry, Genetics and Molecular Biology ,SYMMETRY-BREAKING ,03 medical and health sciences ,0302 clinical medicine ,Gene Expression Regulation, Plant ,Plant Cells ,hemic and lymphatic diseases ,Cell polarity ,lateral diffusion ,Protein phosphorylation ,TRAFFICKING ,PHOSPHORYLATION ,PHOTOTROPISM ,ORGANOGENESIS ,biology ,Indoleacetic Acids ,Agricultural and Biological Sciences(all) ,Arabidopsis Proteins ,Biochemistry, Genetics and Molecular Biology(all) ,polar auxin transport ,Membrane Transport Proteins ,Biology and Life Sciences ,positive feedback ,Biological Transport ,Subcellular localization ,biology.organism_classification ,Cell biology ,protein phosphorylation ,cell polarity ,030104 developmental biology ,Phosphorylation ,plant development ,Polar auxin transport ,General Agricultural and Biological Sciences ,030217 neurology & neurosurgery ,Genetics and Molecular Biology(all) - Abstract
Polarsubcellular localization of the PINexporters of the phytohormoneauxin is a key determinant of directional, intercellular auxin transport and thus a central topic of both plant cell and developmental biology. Arabidopsis mutants lacking PID, a kinase that phosphorylates PINs, or the MAB4/MEL proteins of unknown molecular function display PIN polarity defects and phenocopy pin mutants, but mechanistic insights into how these factors convey PIN polarity are missing. Here, by combining protein biochemistry with quantitative live-cell imaging, we demonstrate that PINs, MAB4/MELs, and AGC kinases interact in the same complex at the plasma membrane. MAB4/MELs are recruited to the plasma membrane by the PINs and in concert with the AGC kinases maintain PIN polarity through limiting lateral diffusion-based escape of PINs from the polar domain. The PIN-MAB4/MEL-PID protein complex has self-reinforcing properties thanks to positive feedback between AGCkinase-mediated PINphosphorylation andMAB4/MEL recruitment.Wethusuncover themolecularmechanism by which AGC kinases and MAB4/MEL proteins regulate PIN localization and plant development.
- Published
- 2021