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IntroductionAn appropriate supply of ammonium (NH4+) in addition to nitrate (NO3−) can greatly improve plant growth and promote maize productivity. However, knowledge gaps exist regarding the mechanisms by which different nitrogen (N) fertilizer sources affect the enzymatic activity of nitrogen metabolism and non-structural carbohydrates during the post-anthesis period.MethodsA field experiment across 3-year was carried out to explore the effects of four nitrateammonium ratio (NO3−/NH4+ = 1:0 (N1), 1:1 (N2), 1:3 (N3), and 3:1 (N4)) on postanthesis dry matter (DM) and N accumulation, partitioning, transportation, and grain yield in maize.ResultsNO3-/NH4+ ratio with 3:1 improved the enzymatic activity of N metabolism and non-structural carbohydrate accumulation, which strongly promoted the transfer of DM and N in vegetative organs to reproductive organs and improved the pre-anthesis DM and nitrogen translocation efficiency. The enzymatic activities of nitrate reductase, nitrite reductase, glutamine synthetase, glutamine oxoglutarate aminotransferase, and non-structural carbohydrate accumulation under N4 treatment were increased by 9.30%–32.82%, 13.19%–37.94%, 4.11%–16.00%, 11.19%–30.82%, and 14.89%–31.71% compared with the other treatments. Mixed NO3−-N and NH4+-N increased the total DM accumulation at the anthesis and maturity stages, simultaneously decreasing the DM partitioning of stem, increasing total DM, DM translocation efficiency (DMtE), and contribution of pre-anthesis assimilates to the grain (CAPG) in 2015 and 2017, promoting the transfer of DM from stem to grain. Furthermore, the grain yield increased by 3.31%–9.94% (2015), 68.6%–26.30% (2016), and 8.292%–36.08% (2017) under the N4 treatment compared to the N1, N2, and N3 treatments.ConclusionThe study showed that a NO3−/NH4+ ratio of 3:1 is recommended for high-yield and sustainable maize management strategies in Northwestern China.

Background and aims: Nitrogen fertilization is one of the most important practices for improving of crop yield and soil quality, which also affects soil CO2 emission. CO2 emission is strongly influenced by soil bacterial communities. It is still unknown precisely how nitrogen fertilization affects soil bacterial communities in the semiarid Loess Plateau and how its effects are related to CO2 emissions. Methods: A field experiment was conducted with four nitrogen fertilization rates (0 (N0), 100 (N1), 200 (N2), and 300 (N3) kg N ha–1). Nitrogen fertilization effects soil bacterial communities, CO2 emission, carbon emission efficiency (CEE), and their relationships were investigated. Results: As a result of nitrogen fertilization, soil bacterial abundance increased, and the bacterial community composition and network changed. Soil CO2 emission under N1, N2, and N3 were increased by 17.3%, 24.8%, and 28.9% compared to the N0 treatment, respectively. N2 and N3 exhibited the highest maize grain yield and CEE. CEE under N2 and N3 increased by 96.9%-140.9% compared to the N0 treatment. Soil bacterial abundance had a positive correlation with CO2 emission. CO2 emission were also affected by the bacterial composition and network, which were related to the Actinobacteria. Multiple regression analysis further suggested that CO2 emission was jointly regulated by the abundance, composition, and network modules of the bacterial community. Conclusion: Overall, our results indicated that nitrogen fertilization promoted CO2 emission via increasing bacterial abundance and altering the composition of their community, and provided a new insight into the connection between soil microorganisms and agricultural CO2 emission in the semi-arid region. [ABSTRACT FROM AUTHOR]

To investigate the effects and mechanism of prolonged inorganic nitrogen (N) fertilization on the N-use efficiency of spring wheat (Triticum aestivum L.), a long-term study initiated in 2003 was conducted. The study analyzed how N fertilization affects dry matter translocation, N translocation, soil NO3-N, and N-use efficiency. Five different N-fertilizer rate treatments were tested: N0, N52.5, N105, N157.5, and N210, corresponding to annual N fertilizer doses of 0, 52.5, 105.0, 157.5, and 210.0 kg N ha−1, respectively. Results showed that increasing N-fertilizer rates significantly enhanced the two-year average dry matter accumulation amount (DMA) at maturity by 22.97–56.25% and pre-flowering crop growth rate (CGR) by 17.11–92.85%, with no significant increase beyond 105 kg N ha−1. However, no significant correlation was observed between the dry matter translocation efficiency (DTE) and wheat grain yield. Both insufficient and excessive N applications resulted in an imbalanced N distribution favoring vegetative growth over reproductive growth, thus negatively impacting N-use efficiency. At maturity, the N-fertilized treatments significantly increased the two-year average N accumulation amount (NAA) by 52.04–129.98%, with no further increase beyond 105 kg N ha−1. N fertilization also improved the two-year average N translocation efficiency (NTE) by 56.89–63.80% and the N contribution proportion (NCP) of wheat vegetative organs by 27.79–57.83%, peaking in the lower-N treatment (N52.5). However, high-N treatment (N210) led to an increase in NO3-N accumulation in the 0–100 cm soil layer, with an increase of 26.27% in 2018 and 122.44% in 2019. This higher soil NO3-N accumulation in the 0–100 cm layer decreased NHI, NUE, NAE, NPFP, and NMB. Additionally, N fertilization significantly reduced the two-year average N harvest index (NHI) by 9.89–12.85% and N utilization efficiency (NUE) by 11.14–20.79%, both decreasing with higher N application rates. The NAA followed the trend of anthesis > maturity > jointing. At the 105 kg N ha−1 rate, the highest N agronomic efficiency (NAE) (9.31 kg kg−1), N recovery efficiency (NRE) (38.32%), and N marginal benefit (NMB) (10.67 kg kg−1) were observed. Higher dry matter translocation amount (DTA) and N translocation amount (NTA) reduced NHI and NUE, whereas higher NTE improved NHI, NUE, and N partial factor productivity (NPFP). Overall, N fertilization enhanced N-use efficiency in spring wheat by improving N translocation rather than dry matter translocation. [ABSTRACT FROM AUTHOR]

Due to depleting water supplies and the cultivation of high water-demanding crops such as lucerne, the effect of water deficits in crop production has become a major concern, especially in semiarid regions of China. A six-year field experiment (2012–2018) was conducted to evaluate soil water recovery and soil fertility after lucerne-to-crop conversions on the western Loess Plateau of China. Six rotation treatments (lucerne initially grew from 2003 to 2012 followed by the rotation of other crops or fallow until assessments in 2018) were: (1) lucerne (Medicago sativa L.)–lucerne (L-L); (2) lucerne–fallow (L-F); (3) lucerne–wheat (Triticum aestivum L.) (L-W); (4) lucerne–corn (Zea mays L.) (L-C); (6) lucerne–potato (Solanum tuberosum L.) (L-P); and (6) lucerne–millet (Setaria italica) (L-M). The same crops were grown each year after cultivation during 2013–2018. According to the findings, all rotation types gradually increased the soil water content, with the 0–110 cm soil layer experiencing the maximum soil water replenishment rate, followed by the 110–200 and 200–300 cm soil layers. After converting lucerne to crops, the amount of organic carbon, total nitrogen, and mineral nitrogen in the soil decreased, whereas total phosphorus and accessible phosphorus increased. Soil bulk density was reduced under rotation treatments. Soil water absolute restoration index was the highest under L-F, followed by L-C, L-W, L-P, and L-M. The rate of soil water recovery was 39.5, 33.0, 33.7, 33.5, 29, and 8.2 mm yr−1 under L-F, L-W, L-C, L-P, L-M, and L-L, respectively. The net economic return was greatest under L-C, followed by L-L, L-W, L-P, and L-M. From the analysis of the long-term experimental results, this study shows that the effect of soil water restoration is greatest when continuous alfalfa is converted into wheat, corn, and potato or fallow after 9 years. When the economic benefits and soil moisture recovery are considered comprehensively, corn sown in a ridge–furrow system with fully plastic film annual mulching is the most suitable field management practice after lucerne-to-crop conversion on the western Loess Plateau of China.

This study evaluated the properties of soil water dynamics and desiccation to a depth of 500 cm and tested the idea that land use affects soil drying in deep profiles. Four land use types were chosen: farmland, artificial forest and grassland, and abandoned land. Soil water content was most outstanding under long-term wheat fields, but average soil water content under artificial vegetation of Caragana korshinskii Kom. and alfalfa dropped to 6–8% within the 160–500 cm soil profile, very near to the 7.0% wilting point. Long-term continuous maize cultivation in a fully mulched ridge–furrow system significantly depleted soil water and resulted in a dried soil layer with a thickness of 240 cm. Short-term or long-term land abandonment enhanced soil reservoir and reduced soil water storage deficit degree compared to long-term maize and artificial vegetation. Soil water storage in the 160–500 cm soil profile was depleted by 240 and 464 mm under long-term maize and Caragana korshinskii Kom., respectively, by 267, 319, 381, and 463 mm under 5-, 10-, 20-, and 30-year alfalfa, and by −58, 278, 234, and 93 mm under 5-, 10-, 20-, and 30-year abandonment land, respectively, compared to long-term wheat. Based on the analysis of long-term experimental results, this study shows that the phenomenon of soil drying caused by long-term intensive maize production cannot be ignored in semi-arid areas and that natural re-vegetation under long-term abandonment, rather than artificial vegetation, may be the best type of vegetation reconstruction for this region based on soil water balances.

Increasing agricultural productivity without undermining further the integrity of the Earth’s environmental systems such as soil water balance are important tasks to ensure food security for an increasing global population in rainfed agriculture. The impact of intercropping maize (Zea mays L.) with potato (Solanum tuberosum L.) on yield, land equivalent ratios (LER), water equivalent ratio (WER), water use, energy output, and net economic return were examined under seven planting systems: potato grown solely or intercropped on the flat field without mulching, maize grown solely or intercropped with potato on ridges or flat field with or without plastic film mulched. The three intercropping systems had 3–13% less water use than the monocropping. Among the intercropped systems, flat field caused more depletion of soil water than ridged field for both years. Compared to monocultures, intercropping with plastic film mulching and ridging significantly increased LER and WER. Meanwhile, intercropping with mulching and ridging significantly increased net economic return and energy output by 8% and 24%, respectively, when compared to monocropping. These results suggest that maize under plastic film mulched ridge-furrow plot intercropped with potato under flat plot without mulching increased energy output, net economic return, and water use efficiency without increasing soil water depletion, which could be an optimal intercropping system for the semiarid farmland on the western Loess Plateau.

Increasing water shortages and environmental pollution from excess chemical nitrogen fertilizer use necessitate the development of irrigation-nitrogen conservation technology in oilseed flax production. Therefore, a two-year split-plot design experiment (2017–2018) was conducted with three types of irrigation (I) levels (no irrigation (I0), irrigation of 1200 m3 ha−1 (I1200), and 1800 m3 ha−1 (I1800)) as the main plot and three nitrogen (N) application rates (0 (N0), 60 (N60) and 120 (N120) kg N ha−1) as the subplot in Northwest China to determine the effects of irrigation and N rates on oilseed flax grain yield, yield components, water-use efficiency (WUE), and N partial factor productivity (NPFP). The results show that I1800 optimized the farmland water storage and water storage efficiency (WSE), which gave rise to greater above-ground biomass. Under I1800, the effective capsule (EC) number increased significantly with increasing irrigation amounts, which increased significantly with increasing nitrogen application rate (0–120 kg ha−1). Both irrigation and nitrogen indirectly affect GY by affecting EC; the highest grain yield was observed at the I1800N60 treatment, which increased by 69.04% and 22.80% in 2017 and 2018 compared with the I0N0 treatment, respectively. As a result, both irrigation and N affect grain yield by affecting soil water status, improving above-ground biomass, and finally affecting yield components. In addition, I1800N60 also obtained a higher WUE and the highest NPFP due to a higher grain yield and a lower N application rate. Hence, our study recommends that irrigation with 1800 m3 ha−1 coupled with 60 kg N ha−1 could be a promising strategy for synergistically improving oilseed flax WUE, grain yield and yield components within this semi-arid region. [ABSTRACT FROM AUTHOR]