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1. Carbapenemase Producers Among Extensive Drug-Resistant Gram-Negative Pathogens Recovered from Febrile Neutrophilic Patients in Egypt

Author

Mabrouk S, Abdellatif G, El-Ansary M, Aboshanab K, and Ragab Y

Subjects
carbapenem resistance, carbapenemases, mdr, xdr, gram negative pathogens, Infectious and parasitic diseases, RC109-216
Abstract

Samar S Mabrouk,1 Ghada R Abdellatif,1 Mona R El-Ansary,2 Khaled M Aboshanab,3 Yasser M Ragab4 1Department of Microbiology, Faculty of Pharmacy, Ahram Canadian University (ACU), 6th of October, Giza, Egypt; 2Department of Biochemistry, Modern University for Technology and Information (MTI), Cairo, Egypt; 3Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University (ASU), Cairo, Egypt; 4Department of Microbiology & Immunology, Faculty of Pharmacy, Cairo University (CU), Cairo, EgyptCorrespondence: Khaled M AboshanabDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., PO: 11566, Abbassia, Cairo, EgyptTel +20 225082595Fax +20 224051107Email aboshanab2012@pharma.asu.edu.egPurpose: This study aimed to detect the prevalence of carbapenemase producers (CPs) among extensive drug-resistant (XDR)-carbapenemase producing Gram-negative bacteria (GNB) recovered from various clinical specimens of hospitalized neutrophilic febrile patients in two major tertiary care hospitals in Egypt.Methods: Standard methods were used to evaluate the antimicrobial susceptibility of clinical isolates according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). Phenotypic and genotypic analysis of CPs were carried out and statistically analyzed using standard methods.Results: Three hundred and forty-two GNB were obtained from 342 clinical specimens during the period of the study, where 162 (47%) were enterobacterial isolates, including, 63 (18.4%) Escherichia coli, 87 (25.4%) Klebsiella spp., 5 (1.46%) Enterobacter cloacae, 5 (1.46%) Salmonella spp. and 2 (0.6%) Proteus and 180 (53%) were non-fermentative bacilli including, 129 (37.7%), Acinetobacter baumannii, and 51 (14.9%), Pseudomonas spp. Out of the 342 GNB, 188 (54.9%) isolates were multi-drug resistant (MDR). Of these, 52 (27.6%) were XDR as well as CPs as confirmed phenotypically. The MIC of imipenem against the XDR GNB against showed either low (11 isolates; 21.1%; MIC range =4– 32 μg/mL) or high levels of resistance (41 isolates; 78.8%; MIC range = 64-≥ 1024). The most prevalent carbapenem resistance (CR) genes were blaKPC (63.5%) followed by blaOXA-48 (55.7%) and blaVIM (28.8%). No significant association could be observed between the MIC level and the presence of CR genes (P value > 0.05).Conclusion: High prevalence of MDR (54.9%) and XDR (27.6%) GNB pathogens associated with high levels of resistance to carbapenems were observed. All XDR GNB were CPs and tested positive for at least one of the CR genes. However, most of them (78.8%) showed a high level of CR (MIC range = 64-≥ 1024) with no significant association with the CR genes.Keywords: carbapenem resistance, carbapenemases, MDR, XDR, Gram-negative pathogens

Published
2020

11. Non-Invasive Predictors for the Presence , Grade and Risk of Bleeding from Esophageal Varices in Patients with Post-Hepatitic Cirrhosis

Author

El-Talkawy, El-Aleem Aa, El Ansary M, Safeem Aa, El-Aziz Im, Azab Mm, El-Badea Ma, El Ray A, and Diab Tm

Subjects
Adult, Liver Cirrhosis, Male, medicine.medical_specialty, Cirrhosis, Portal venous pressure, Serum albumin, Esophageal and Gastric Varices, Gastroenterology, Esophageal varices, Risk Factors, Internal medicine, Humans, Medicine, Endoscopy, Digestive System, biology, business.industry, Albumin, Middle Aged, Bleed, medicine.disease, biology.protein, Portal hypertension, Female, Gastrointestinal Hemorrhage, business, Varices
Abstract

Variceal bleeding is the last step of a chain of events initiatedby an increase in portal pressure, followed by the development and progressive dilation ofvarices until these finally rupture and bleed. The ideal method to diagnose portal hypertension should be accurate, noninvasive, objective, and reproducible. The study evaluated the predictive value of two non-invasive parameters for the diagnosis of esophageal varices (EV): 1-Right liver lobe diameter/serum albumin ratios (RLLD/S. albumin), and 2-Platelet count/splenic bipolar diameter ratios (Platelets count/SBPD). This study included eighty Egyptian patients with post-hepatitic cirrhosis (45 males and 35 females). They underwent laboratory ultrasono-graphic and endoscopic examinations within one week. RLLD/S. albumin and Platelets count/SBPD ratios were calculated. The results showed that EV were not detected by upper digestive endoscopy in 25%, while grade I of EV was found in 17.5%, grade II in 17.5%, grade III in 20%, & grade IV in 20%. RLLD/S. albumin concentration ratio diagnosed the varices at cut off value of 3.43 with 95% sensitivity and 80% specificity. Also, it was positively correlated with grading of E.V, when this ratio increased the grading of E.V increases and vice versa. Besides, it predicted bleeding from E.V. at cut off value of 5.096 with 63% sensitivity and 73% specificity. Platelet count/SBPD ratio predicted the presence of varices at cut off value 1847 with 95% sensitivity and 93% specificity, and negatively correlated with grading of EV, when this ratio decreased grading of E.V increase and vice versa. It also predicted bleeding from E.V. at cut off value of 4809 with 50% sensitivity and 93% specificity.

Published
2015

13. Leucocyte Malignancies

Author

Andrews, R. G., Bernstein, I. D., Torok-Storb, B., Bell, C. G., Biberfeld, P., Christensson, B., Öst, Å., Hast, R., Skoog, L., Andreason, R., Olsson, L., Lagerlöf, B., Reizenstein, P., Boucheix, C., Perrot, J. Y., Mirshahi, M., Fournier, N., Billard, M., Giannoni, F., Bernadou, A., Rosenfeld, C., Chiao, J. W., Wang, C. Y., Del Giacco, G. S., Cengiarotti, L., Di Tucci, A., Broccia, G., Corda, G., Mathieu, A., Indiveri, F., Locci, F., Mantovani, G., Detrick-Hooks, B., Boumsell, L., Bernard, A., Hooks, J. J., Divine, M., Farcet, J. P., Gourdin, M. F., Vasconcelos, A., Tabilio, A., Andre, C., Bouguet, J., Reyes, F., Fontan, S., Zabay, J. M., Garcia, M. C., Pascual-Salcedo, D., Campos, A., de la Concha, E. G., Gordon, J., Åman, P., Mellstedt, H., Klein, G., Habeshaw, J. A., Herrmann, F., Sieber, G., Rühl, H., Lebacq, A. M., Ravoet, A. M., Bazin, H., De Bruyere, M., Rodhain, J., Sokal, G., Losa, G., Maestroni, G., Lucivero, G., Antonaci, S., Stomeo, C., Bonomo, L., Magaud, J. P., Brochier, J., Vu Van, H., El Ansary, M., Gentilhomme, O., Bryon, P. A., Mathé, G., Minowada, J., Ogier, C., Ginsbourg, M., Goutner, A., Knapp, W., Canon, C., Melief, C. J. M., Miedema, F., Rümke, H. C., ten Berge, R. J. M., Terpstra, F. G., v.d. Borne, A. E. G. Kr., Willemze, R., v. Vloten, W., Miljkovic, A. K., Isakovic, K., Jankovic, B. D., Peng, R., Knowles, D. M., Bushkin, Y., Petrányi, G. G., Busch, G., Milford, E., Todd, R., Griffin, J., Reinherz, E., Terhorst, C., Schlossman, St., Carpenter, Ch., Richard, Y., Demeocq, F., Schwarting, R., Thoen, J., Førre, Ø. T., von Fliedner, V., Carrel, S., Heumann, D., Zaech, P., Sekaly, R. P., Girardet, Ch., Mach, J. P., Azzo, W., Worman, C., Mills, K., Brooks, D., Hogg, N., Zola, H., Beverly, P., Cawley, J., Bernard, Alain, editor, Boumsell, Laurence, editor, Dausset, Jean, editor, Milstein, César, editor, and Schlossman, Stuart F., editor

Published
1984
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24. Potential of Plant-Parasitic Nematode Control in Banana Plants by Microalgae as a New Approach Towards Resistance.

Author

Hamouda, Ragaa A. and El-Ansary, M. S. M.

Subjects
*BANANAS, *NEMATODES as biological pest control agents, *MICROALGAE, *ROOT-knot nematodes, *CHLORELLA vulgaris, *FOURIER transform infrared spectroscopy
Abstract

Effect of algal derivatives or their components on suppression of the root-knot nematode (RNK, Meloidogyne incognita) has few studies. So, the application of the nematicidal potential of 3 microalgae, Scenedesmus obliquus (SO), Chlorella vulgaris (CV) and Anabaena oryzae (AO), alone or in combination were tested against M. incognita on banana plants grown in 30 cm pots soil. All algal tested materials significantly (p < 0.05) reduced the number of second stage juveniles (J2s) in the soil (52.6%), compared with the inoculated check. Similarly, the algal treatment (AO + CV) was found to be the most clearly positive effect on reducing of J2s of M. Incognito in the soil comparing to those of control with nematodes. However, the presented plant girth (shoot weight, shoot length, root weight, root length, corm weight) had a maximum impression in the all tested materials. Shoot weight of most plants was much higher than that of the check. Interestingly, the obtained results from Fourier transform infrared spectroscopy (FT-IR) denoted that the chemical content of the tested plants changed according to various treatments. The highest components of total phenolics and the highest antioxidant vigor were found in the plants treated with AO + CV + SO. Overall, the previous algal treatments were forceful protector and had a potent stimulating effect on the plant girth and inhibition of M. incognita in susceptible banana plants. [ABSTRACT FROM AUTHOR]

Published
2017

32. 37-THE PHOSPHORUS CONTENT OF EGYPTIAN RAW COTTON.

Author

Wahbi, A. K. and El-Ansary, M. S.

Abstract

The average phosphorus content of the present varieties of Egyptian raw cotton, determined by a simple colorimetric method, is found to be 0∙113 per cent phosphorus pentoxide. The corresponding figure for the old varieties, examined by Geake in 1923, is 0∙109 per cent phosphorus pentoxide. The phosphorus content increases with the staple length. The effect of the available phosphoric acid content of the soil on the phosphorus content of raw cotton is partially investigated and the relation between phosphorus content and Methylene Blue number for the raw and bleached cotton respectively is examined. The efficiency of water, dilute caustic soda and dilute sulphuric acid in removing phosphorus compounds from raw cotton, under certain conditions, is determined. [ABSTRACT FROM AUTHOR]

Published
1954
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36. Feasibility study estimation of in-situ resources and recoverable reserves for the Boddington gold mine.

Author

Collings P.S., Resources and reserves symposium Sydney Nov-87Nov-87, El-Ansary M., Collings P.S., Resources and reserves symposium Sydney Nov-87Nov-87, and El-Ansary M.

Abstract

The process of ore reserve estimation is discussed. The added complication that each of the joint partners had a different perception of the deposit is considered., The process of ore reserve estimation is discussed. The added complication that each of the joint partners had a different perception of the deposit is considered.

Published
1987

42. Boddington gold mine.

Author

El-Ansary M., Collings P., El-Ansary M., and Collings P.

Abstract

Primary mineralisation intersected is finely disseminated or associated with quartz veins and consists of pyrite, pyrrhotite, chalcopyrite, minor molybdenite, arsenopyrite and scheelite and rare free gold, tetrahedrite and stibnite. The only significant mineralisation occurs in the intermediate to felsic volcanic and the diorite intrusives., Primary mineralisation intersected is finely disseminated or associated with quartz veins and consists of pyrite, pyrrhotite, chalcopyrite, minor molybdenite, arsenopyrite and scheelite and rare free gold, tetrahedrite and stibnite. The only significant mineralisation occurs in the intermediate to felsic volcanic and the diorite intrusives.

43. Geochemical patterns in the laterite profile at the Boddington gold deposit, Western Australia.

Author

Davy R., El-Ansary M., Davy R., and El-Ansary M.

Abstract

In the profiles studied, components can be divided into those which are generally leached (Ba, Co, La, Mn, Ni, Rb, Sr, Ta, Y, Zn, CaO, K2O, MgO, and Na2O); those which are mobilised and reprecipitated within the profile (Au, Ce, Cr, Cu, Mo, and Fe2O3) and those which are essentially residual (Nb, Sn, Th, V, W, Zr, A12O3 and TiO2). Residual components have been mobilised in the profile but have not been transported any great distance. Anomalous concentrations of As, Au, Cr, Cu, Mo, Nb, Sn, Th, V and W, retained at the surface within recognisable lateral distance (100-150 m) of their bedrock sources, appear suitable as either direct or indirect indicators of bedrock mineralisation. Significant concentration of gold occurs within the lower part of the lateritic hardcap and upper part of the underlying nodular B-zone. Local concentrations of gold lower in the profile are attributed to still-stands of the water table during periods of generally increasing aridity over the last 30 million years., In the profiles studied, components can be divided into those which are generally leached (Ba, Co, La, Mn, Ni, Rb, Sr, Ta, Y, Zn, CaO, K2O, MgO, and Na2O); those which are mobilised and reprecipitated within the profile (Au, Ce, Cr, Cu, Mo, and Fe2O3) and those which are essentially residual (Nb, Sn, Th, V, W, Zr, A12O3 and TiO2). Residual components have been mobilised in the profile but have not been transported any great distance. Anomalous concentrations of As, Au, Cr, Cu, Mo, Nb, Sn, Th, V and W, retained at the surface within recognisable lateral distance (100-150 m) of their bedrock sources, appear suitable as either direct or indirect indicators of bedrock mineralisation. Significant concentration of gold occurs within the lower part of the lateritic hardcap and upper part of the underlying nodular B-zone. Local concentrations of gold lower in the profile are attributed to still-stands of the water table during periods of generally increasing aridity over the last 30 million years.

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