1. Bioremediation of crystal violet using air bubble bioreactor packed with Pseudomonas aeruginosa.
- Author
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El-Naggar MA, El-Aasar SA, and Barakat KI
- Subjects
- Animals, Anti-Infective Agents, Local isolation & purification, Anti-Infective Agents, Local toxicity, Artemia, Biodegradation, Environmental, Gentian Violet isolation & purification, Gentian Violet toxicity, Lethal Dose 50, Anti-Infective Agents, Local metabolism, Bioreactors, Gentian Violet metabolism, Pseudomonas aeruginosa physiology, Water Purification methods
- Abstract
Seven water and sediment samples were collected and tested for decolorizing crystal violet. Pseudomonas aeruginosa was the most effective isolate for dye decolorization. The LC(50) of the crystal violet (115 mg/l) was measured using Artemia salina as a biomarker. The effect of different heavy metals on crystal violet decolorization was investigated. Cd(2+) and Fe(3+) ions showed marginal enhancement of the decolorization process, the rate was 1.35 mg/l/h compared to 1.25 mg/l/h for the control. Phenol and m-cresol showed no effect on crystal violet decolorization, meanwhile p-cresol and p-nitrophenol reduced the decolorization rate to 1.07 and 0.01 mg/l/h, respectively. P. aeruginosa cells were immobilized by entrapment in agar-alginate beads. The beads were cultivated and reused in Erlenmeyer flask and in an air bubble column bioreactor and they enhanced the crystal violet decolorization rate to 3.33 and 7.5 mg/l/h, respectively.
- Published
- 2004
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