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Protein hydrolysates are composed of peptones and peptides, and their type depends on the biological source and manufacturing process. We studied the survival response of Salmonella enterica in the presence of casein, soy protein, and fish protein hydrolysates at refrigeration and freezing temperatures. Fish protein hydrolysate (FPH) was identified as the only capable hydrolysate of sustaining the viability of S. enterica for 60 days at refrigeration temperatures. None of the three hydrolysates were able to impart cryoprotection to S. enterica under freezing conditions. The survival rate of S. enterica in FPH was further enhanced by growing the inoculum on agar instead of broth. An optimization study using response surface methodology was also conducted to identify suitable concentrations of protein hydrolysates capable of maximizing the survival of S. enterica. The results of this study can be used to manage Salmonella-related food safety incidents by replacing animal-sourced with plant-sourced protein hydrolysates at the recipe development stage, as well as the use of FPH in microbiological growth media and the formulation of secondary reference materials. These results indicate that FPH produced by enzymatic hydrolysis of Stolephorus indicus at lower concentrations (5%) can serve as nutritive agents in culture media, contribute to long-term preservation, and can be used in the formulation of quantitative reference materials for Salmonella.

In countries where farming is largely subsistence, no pedigree records of farm animals are maintained at farmers' herd and scientific mating plans are not observed which leads to the accumulation of inbreeding and loss of production potential. Microsatellites have been widely used as reliable molecular markers to measure inbreeding. We attempted to correlate autozygosity estimated from microsatellite data with the inbreeding coefficient (F) calculated from pedigree data in Vrindavani crossbred cattle developed in India. The inbreeding coefficient was calculated from the pedigree of ninety-six Vrindavani cattle. Animals were further grouped into three groups viz. acceptable/low (F: 0–5%), moderate (F: 5–10%) and high (F: ≥10%), based on their inbreeding coefficients. The overall mean of the inbreeding coefficient was found to be 0.070 ± 0.007. A panel of twenty-five bovine-specific loci were chosen for the study according to ISAG/FAO. The mean FIS,FST, and FIT values were 0.0548 ± 0.025, 0.012 ± 0.001 and 0.0417 ± 0.025, respectively. There was no significant correlation between the FIS values obtained and the pedigree F values. The locus-wise individual autozygosity was estimated using the method-of-moments estimator (MME) formula for locus-specific autozygosity. The autozygosities ascribing to CSSM66 and TGLA53 were found to be significantly (p <.01 and p <.05, respectively) correlated with pedigree F values. [ABSTRACT FROM AUTHOR]

In the present study, the gelatin from fresh croaker fish (Johnius sp) skin was obtained by rapid extraction and its properties were assessed. The gelling and melting temperature of croaker skin gelatin was 17.4 and 23.8°C as revealed by dynamic visco-elastic analysis using Controlled Stress Rheometer. Croaker skin gelatin (CSG) had the bloom value of 193.4 g. Scanning electron microscopic analysis of freeze dried gelatin showed the presence of voids surrounded by a loose network imitating a sponge or coral like structure. Texture profile parameters such as hardness, springiness, cohesiveness, gumminess, and chewiness of croaker fish skin gelatin gel were relatively lower than the porcine gelatin gel. The amino acid composition of gelatin revealed a higher proportion of glycine, alanine, and hydroxyl proline (30.25, 23.4, and 11.82 g/100 g protein, respectively) content. The electrophoretic analysis showed the presence of two peptide chains with the approximate molecular weight of 200 and 116 kDa corresponds to β and α peptide chain of collagen. The Fourier Transform Infrared (FT-IR) spectra of gelatin showed peaks correspond to the structures like α helix, β sheet, and random coil in the gelatin preparations. [ABSTRACT FROM AUTHOR]

In the present investigation, biochemical changes in dressed and frozen storedPriacanthus hamrurand their influence on quality of fish sausage have been studied. During the frozen storage period of five months, moisture content decreased and led to the increment on other proximate constituents due to percentage balance. Quality indices such as total volatile base nitrogen and trimethyl amine nitrogen increased significantly (P< 0.05) and reached 44.80 mg N/100 g and 22.00 mg N/100 g of meat, respectively. Non-protein nitrogenous contents were found to decrease due to the loss in thaw drip. Lipid freshness parameters such as peroxide value and free fatty acid value increased and resulted in reduced extractability of salt soluble proteins. Gel strength of fish sausage decreased significantly (P< 0.05) during the storage period and this was also supported by the folding test. Increments in the expressible moisture content clearly indicated the loss of protein functionality, like water holding ability, due to denaturation during frozen storage. From the sensory evaluation, dressedP. hamrurcan be stored frozen without any cryoprotective and preservative agents up to four months when it is intended for the preparation of fish sausage and this was also evidence from the changes in biochemical constituents. [ABSTRACT FROM PUBLISHER]

Fish protein hydrolysates were prepared from Catla catla using different proteases viz. alcalase, bromelain, flavorzyme and protamex and designated as HA (alcalase), HB (bromelain), HF (flavorzyme) and HP (protamex), respectively. HB was found to have higher 2, 2 diphynyl-1-picrylhydrazyl ( DPPH) free radical-scavenging activity followed by HF, HP and HA. HB had higher ferric-reducing power followed by HP, HF and HA. Linoleic acid peroxidation inhibition of HA, HB, HF and HP were 54 ± 4.75, 50 ± 2.60, 49 ± 3.00 and 36 ± 6.15%, respectively. Higher emulsion stability index and foaming capacity were recorded for HB. The emulsion activity index was higher in HP (52.76 ± 1.27 m2/g). The foaming stability was found to be good in HF. Practical Application The properties of fish protein hydrolysates from Catla catla using different enzymes have been studied. The protein hydrolysates prepared from C. catla can be used as antioxidant and functional ingredient in the processed food products. Among four different enzymes used, bromelain was effective in obtaining the protein hydrolysate with desired antioxidant and functional properties. The potential to use bromelain for hydrolysate preparation is high considering the abundance of pineapples in southern India. [ABSTRACT FROM AUTHOR]

In vitro studies were undertaken aiming to study the methane (CH 4 ) mitigation potential of biowaste (BW) of Padina gymnospora at the graded inclusion of 0% (C), 2% (A 2 ), 5% (A 5 ), and 10% (A 10 ) of the diet composed of straw and concentrate in 40:60 ratio. The chemical composition analysis revealed that the BW contained higher crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and ether extract (EE) than the PF (fresh seaweed, P. gymnospora ). The concentration of cinnamic acid, sinapic acid, kaempferol, fisetin p-coumaric acid, ellagic acid, and luteolin in BW was 1.5-6-folds less than the PF. Inclusion of BW decreased ( P < 0.0001) CH 4 production by 34%, 38%, and 45% in A 2 , A 5 , and A 10 treatments, respectively. A decrease ( P < 0.0001) of 7.5%-8% in dry matter (DM) and organic matter (OM) digestibility was also recorded with the BW supplementation. The BW inclusion also decreased the numbers of total ( P = 0.007), Entodinomorphs ( P = 0.011), and Holotrichs ( P = 0.004) protozoa. Metagenome data revealed the dominance of Bacteroidetes, Proteobacteria, Firmicutes, Actinobacteria, and Fibrobacter microbial phyla. At the phylum level, Euryarchaeota dominated the archaeal community, whereas Methanobrevibacter was most abundant at the genus level. It can be concluded that the inclusion of BW in straw and concentrate based diet by affecting rumen fermentation, protozoal numbers, and compositional shift in the archaeal community significantly decreased CH 4 production. Utilization of biowaste of P. gymnospora as a CH 4 mitigating agent will ensure its efficient utilization rather than dumping, which shall cause environmental pollution and health hazards., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Mohapatra, Trivedi, Kolte, Tejpal, Elavarasan, Vaswani, Malik, Ravishankar and Bhatta.)

Background: Postoperative pain management is vital to improve patient care. Successful postoperative pain relief is currently achieved only through NSAIDs and narcotics., Objectives: We compared dexmedetomidine and clonidine as additives to hyperbaric levobupivicaine 0.5% for the sub-arachnoid block (spinal anesthesia) concerning the onset time, duration of the block, hemodynamic changes, level of sedation intraoperatively and postoperatively and time taken for the first postoperative analgesic request and frequency., Methods: This prospective, double-blind study enrolled 60 patients who underwent lower abdominal surgeries and were eligible for a sub-arachnoid block. They were allocated randomly to one of the two groups. Group D received intrathecal dexmedetomidine 5 µg and 0.5% hyperbaric levobupivicaine 15 mg. Group C received intrathecal clonidine 50 µg and 0.5% hyperbaric levobupivicaine 15 mg., Results: Patients who received dexmedetomidine had a longer duration of the block (2-segment regression: 135 ± 15 min vs. 130 ± 20 min, S1 segment regression: 305 ± 50.4 min vs. 290 ± 47.2 min, Bromage 0: 285 ± 60 min vs. 280 ± 45 min), delayed first rescue analgesia request (700 ± 160 min vs. 506 ± 112 min), reduced frequency of rescue analgesics (1 vs. 2), and desired level of sedation (1.3 ± 0.46 vs. 0.4 ± 0.01) when compared to those receiving clonidine. There were insignificant differences between the groups in intraoperative hemodynamic parameters, such as minimal bradycardia and minimal hypotension. Though dexmedetomidine had an early onset, there was no statistically significant difference compared to clonidine., Conclusions: Comparing dexmedetomidine and clonidine as additives in the sub-arachnoid block, the group who received dexmedetomidine had similar onset, prolonged duration of blockade, delayed first rescue analgesia demand, reduced frequency of analgesics, and desired sedation with similar minimal hemodynamic changes such as bradycardia and hypotension., Competing Interests: The authors declare that they have no conflict of interest., (Copyright © 2023, Murali Manoj et al.)

Background: Transversus abdominis plane (TAP) block is one of the novel approaches and an effective method for providing postoperative analgesia in patients undergoing lower abdominal surgeries., Objectives: To evaluate the efficacy and routine usage of TAP block for postoperative analgesia in different lower abdominal surgeries., Methods: It is a randomized, double-blind trial. Sixty patients undergoing lower abdominal surgeries in sub-arachnoid block with bupivicaine 0.5% were randomized to undergo TAP block (n = 30) using ropivacaine 0.375% as the study group. In the control group (n = 30), only the standard analgesic regimen (paracetamol 1 gm IV and tramadol 50 mg IV) was given. Postoperative pain was assessed using Visual Analogue Scale (VAS) at 30 min and 4, 8, 12, 16, 20, and 24 hrs. First analgesic request after TAP block was compared with a subsequent number of analgesics given postoperatively. Rescue analgesia given postoperatively at request after TAP block was paracetamol 1 gm IV and tramadol 50 mg IV., Results: Patients who received TAP block had a significant reduction in postoperative pain scores at 30 min - 0 (0 - 1), 4 hrs - 0 (0 - 4), 8 hrs - 4 (3 - 5), 12 hrs - 1 (0 - 4), 16 hrs - 1 (1 - 2), 20 hrs -1 (0 - 2), 24 hrs -1 (0 - 1) with P-value < 0.05 in the first 24 hrs. TAP block also delayed the first rescue analgesic request (265 ± 24 min Vs. 66 ± 15 min with P-value < 0.005) and reduction in subsequent analgesic requirements in the first 24 hrs (1.04 ± 0.26 Vs. 2.3 ± 0.48 with P-value < 0.05)., Conclusions: TAP block holds a considerable part in postoperative analgesia. Highly effective for the first 24 hrs in patients undergoing different lower abdominal surgeries, it delays the first rescue analgesic request, decreases the subsequent analgesic requirement, and augments early mobilization, discharge, and cost-effectiveness., Competing Interests: Conflict of Interests: The authors declare no conflict of interest., (Copyright © 2023, Author(s).)

Fish protein hydrolysates (FPH) from fresh water carps Catla catla , Labeo rohita and Cirrhinus mrigala were prepared with 5, 10, 15 and 20% degree of hydrolysis (DH) using papain enzyme. FPH were evaluated for antioxidant properties using in vitro assays such as DPPH free radical scavenging activity (at 10 mg/ml), ferric reducing antioxidant power assay (at 20 mg/ml) and linoleic acid peroxidation inhibition activity (at 10 mg/ml). Antioxidant properties of FPH varied with species and DH. The DPPH radical scavenging activity, linoleic acid peroxidation inhibition activity and ferric reducing antioxidant power (as absorbance at 700 nm) of FPH from carps was in the range of 59-92%, 52-85% and 0.388-0.663 respectively. Based on the overall antioxidant activity, FPH from C. catla with 20% DH was added to oil sardine mince at different concentration (0.1, 0.2 and 0.4%) and found to inhibit effectively the formation of peroxides and malonaldehyde in dose dependent manner. FPH from C. catla with 20% DH was fractionated using size exclusion chromatography and had three different peptide fractions with the approximate molecular weight of 6561-2106 Da (fraction 1), 1942-994 Da (fraction 2) and 935-383 Da (fraction 3). The present study showed promising results that the fish protein hydrolysates from fresh water carps muscle proteins can be used as natural antioxidants in food system. Production of fish protein hydrolysates with nutraceutical properties could be the way forward for better utilization and value addition., Competing Interests: Conflict of interestAuthors do not have any conflict of interest in the presented research work., (© Association of Food Scientists & Technologists (India) 2021.)

Developing peptide-based drugs are very promising to address many of the lifestyle mediated diseases which are prevalent in a major portion of the global population. As an alternative to synthetic peptide-based drugs, derived peptides from natural sources have gained a greater attention in the last two decades. Aquatic organisms including plants, fish and shellfish are known as a rich reservoir of parent protein molecules which can offer novel sequences of amino acids in peptides, having unique bio-functional properties upon hydrolyzing with proteases from different sources. However, rather than exploiting fish and shellfish stocks which are already under pressure due to overexploitation, the processing discards, regarded as secondary raw material, could be a potential choice for peptide based therapeutic development strategies. In this connection, we have attempted to review the scientific reports in this area of research that deal with some of the well-established bioactive properties, such as antihypertensive, anti-oxidative, anti-coagulative, antibacterial and anticarcinogenic properties, with reference to the type of enzymes, substrate used, degree of particular bio-functionality, mechanism, and wherever possible, the active amino acid sequences in peptides. Many of the studies have been conducted on hydrolysate (crude mixture of peptides) enriched with low molecular bioactive peptides. In vitro and in vivo experiments on the potency of bioactive peptides to modulate the human physiological functions beneficially have demonstrated that these peptides can be used in the prevention and treatment of non-communicable lifestyle mediated diseases. The information synthesized under this review could serve as a point of reference to drive further research on and development of functionally active therapeutic natural peptides. Availability of such scientific information is expected to open up new zones of investigation for adding value to underutilized secondary raw materials, which in turn paves the way for sustainability in fish processing. However, there are significant challenges ahead in exploring the fish waste as a source of bioactive peptides, as it demands more studies on mechanisms and structure-function relationship understanding as well as clearance from regulatory and statutory bodies before reaching the end user in the form of supplement or therapeutics.

Fish protein hydrolysates (FPHs) were prepared from freshwater carps ( Catla catla , Labeo rohita , and Cirrhinus mrigala ) using flavorzyme at different degrees of hydrolysis (DHs) ranging from 5 to 20%. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical-scavenging activity of the FPHs prepared from the three species were in the range of 50-82%; the ferric reducing power of the FPHs prepared from catla was the highest. The linoleic acid peroxidation inhibition activity of the prepared FPHs varied from 71 to 91%. The emulsion activity index of the FPHs prepared from catla and rohu decreased significantly with an increase in the DH ( p  < 0.05). The emulsion stability index of the FPHs prepared from the three species was the highest at 20% DH. FPHs prepared from freshwater carps possess good antioxidant and surface-active properties and are therefore suitable to be used as natural antioxidants in health-food formulation and as water-soluble antioxidants in the food-processing industry., Competing Interests: Compliance with ethical standardsThe authors declare no conflict of interest.