1. Chondroitin/dermatan sulfate purified from corb (Sciaena umbra) skin and bone: In vivo assessment of anticoagulant activity
- Author
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Ali Bougatef, Zouheir Sahnoun, Zohra Ghlissi, Hajer Bougatef, Rim Kallel, Ikram Ben Amor, T. Boudawara, Nicola Volpi, Assaad Sila, and Jalel Gargouri
- Subjects
Drug Evaluation, Preclinical ,Dermatan Sulfate ,02 engineering and technology ,Thrombin time ,Biochemistry ,Bone and Bones ,Dermatan sulfate ,03 medical and health sciences ,chemistry.chemical_compound ,Liver Function Tests ,X-Ray Diffraction ,Structural Biology ,In vivo ,medicine ,Animals ,Chondroitin ,Chondroitin sulfate ,Rats, Wistar ,Molecular Biology ,Glycosaminoglycans ,Skin ,030304 developmental biology ,Anticoagulant activity ,Sciaena umbra ,Toxicological studies ,0303 health sciences ,Chromatography ,Calorimetry, Differential Scanning ,medicine.diagnostic_test ,Chondroitin Sulfates ,Fishes ,Anticoagulants ,Electrophoresis, Cellulose Acetate ,General Medicine ,021001 nanoscience & nanotechnology ,Cellulose acetate ,Oxidative Stress ,Liver ,chemistry ,Toxicity ,Microscopy, Electron, Scanning ,Female ,Blood Coagulation Tests ,0210 nano-technology ,Partial thromboplastin time - Abstract
The present work deals with the extraction and purification of chondroitin sulfate/dermatan sulfate from skin (CSG) and bone (CBG) of corb (Sciaena umbra). Electrophoresis of these polymers in barium acetate buffer on cellulose acetate revealed two fractions similar to dermatan sulfate and chondroitin sulfate. The in vivo anticoagulant activity of both chondroitin sulfate/dermatan sulfate (CS/DS) were evaluated, at 25 and 75 mg kg−1 of body weight (b.w), using activated partial thromboplastin time (aPTT), prothrombine time (TT) and thrombin time (PT) tests. Results showed that aPTT of CSG and CBG at 75 mg kg−1 of b.w were prolonged by 1.59 and 1.48-fold respectively, compared with the control. Further, toxicity studies on liver performed by the catalytic activity of transaminases in plasma, oxidative stress markers and hepatic morphological changes demonstrated that CSG and CBG at both doses are not toxics. In summary, the higher activity and lower toxicity of both CS/DS, especially at 25 mg kg−1 of b.w, recommended these compounds as a better drug candidate.
- Published
- 2020