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Probiotics are live microorganisms that promote the selective growth of beneficial bacteria in birds and can be an effective alternative to growth-promoting antibiotics (GPAs) in poultry. The present study was conducted to determine the effects of dietary probiotics in the diet of early phase laying hens on laying performance, egg quality, blood parameters, and organs morphological characteristics. Seventy-two 13-week-old Lohman White hens were randomized into three dietary groups feed diets with GPA (0.5% of COLI-ZIN), probiotic (PRO; 0.1% Bifidobacterium animalis ssp lactis) or without additive (control; CON) for a 90-days feeding trial. Significant differences were observed in feed intake and feed conversion ratio in PRO with respect to GPA and CON at 90-days treatment (pBifidobacterium animalis ssp lactis supplementation can be used as a safe alternative in young laying hens.