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The main objective of the present work was to assess the ecotoxicological safety of the use of thermochemically treated sewage sludge from the wastewater treatment plant (WWTP) of a distillery plant as a soil additive in agricultural soils based on its physicochemical characteristics and the bioaccumulation of selected elements in the plant tissues of maize (Zea mays). We have carried out physicochemical characterization (pH, EC, Corg, Cinorg, CEC, N, H, ash content, PAHs) of sewage sludge feedstock (SS) and sludge-derived biochar (BC) produced by slow pyrolysis at a temperature of 400 °C. The effect of 1% (w/w) amendment of SS and BC on soil physicochemical properties (pH, EC, Cinorg), germination of ryegrass, soil rhizobacteria and microorganisms, as well as on the accumulation and translocation of selected elements in maize (Zea mays) was studied. The results show that pyrolysis treatment of distillery WWTP sludge at 400 °C increases pH (from 7.3 to 7.7), Corg(from 28.86% to 36.83%), N (from 6.19% to 7.53%), ash content (from 23.59% to 50.99%) and decreases EC (from 2.35 mS/cm to 1.06 mS/cm), CEC (from 118.66 cmol/kg to 55.66 cmol/kg), H (from 6.76% to 1.98%) and Σ18 PAHs content (from 4.03 mg/kg to 3.38 mg/kg). RFA analysis of SS and BC showed that pyrolysis treatment multiplies chromium (Cr) (2.2 times), nickel (Ni) (2.96 times), lead (Pb) (2.13 times), zinc (Zn) (2.79 times), iron (Fe) (1.26 times) in the obtained BC, but based on an ecotoxicological test with earthworms Eisenia fetida, we conclude that pyrolysis treatment reduced the amount of available forms of heavy metals in BC compared to SS. We demonstrated by a pot experiment with a maize that a 1% addition of BC increased soil pH, decreased EC and Cinorg and had no significant effect on heavy metal accumulation in plant tissues. According to the results of the three-level germination test, it also does not affect the germination of cress seeds (Lepidium sativum). There was a significant effect of 1% BC addition on soil microbial community, and we observed a decrease in total microbial biomass and an increase in fungal species variability in the soil. Based on these results, we conclude that BC represents a promising material that can serve as a soil additive and source of nutritionally important elements after optimization of the pyrolysis process.

Thin-layer chromatography (TLC) is an often omitted analytical technique due to its lower sensitivity and separation capacity. Even in the era of high-performance liquid chromatography (HPLC), thin-layer chromatography still offers many advantages, such as simplicity, rapidity, and cost-effectiveness, which predict TLC to be the first-choice method for the laborious optimization process requiring analysis of numerous samples. In this work, a thin-layer chromatography method with chemical and densitometric detection was used to optimize a dispersive liquid-liquid microextraction (DLLME) process for the extraction and preconcentration of estradiol in human urine. The chromatographic system consisted of silica gel plates as the stationary phase and toluene-ethanol (9:1; v/v) mixture as the developing solvent. The plates were dyed with 10% phosphomolybdic acid reagent and sequentially evaluated densitometrically at λ = 430 nm. In the context of DLLME optimization, parameters including the type and volume of extraction and dispersive solvents, centrifugation, salt addition and extraction time, were studied. The proposed DLLME-TLC method was successfully applied to the determination of estradiol in real human urine samples.