39 results on '"Elie-Caille, Céline"'
Search Results
2. Lamb waves sensor in liquid media utilizing higher-order quasi-longitudinal S5 and S6 modes
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Hamidullah, Muhammad, Rezzag, Nassim, Élie-Caille, Céline, and Leblois, Thérèse
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Physics - Applied Physics - Abstract
Higher-order quasi-longitudinal (QL) Lamb waves have been reported for sensor application in liquid media at a higher thickness-to-wavelength (h/l) ratio than the fundamental symmetric modes, with the main advantage of having higher operating frequency and reduction in the total size of the device without reducing the plate thickness. However, the trade-off in the reduction of the electromechanical coupling coefficient (K2) is a hinder in utilizing QL Lamb waves with higher-order numbers. Here we performed finite element simulation and verified experimentally the possibility of utilizing S5 and S6 QL Lamb wave modes in liquid media based on a two-port delay line device on a thick GaAs substrate. While the K2 values are lower for QL modes at higher-order numbers, the signal amplitude of S21 transmission parameter at the resonant frequency is still distinguishable from the noise floor. The result demonstrates the feasibility of higher-order modes at a higher h/l ratio for sensor application in liquid media, thus allowing further reduction in the wavelength and the total size of the sensor device.
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- 2021
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3. Deposition and characterization of ZnO thin films on GaAs and Pt/GaAs substrates
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Chawich, Juliana, Kuprenaite, Sabina, Margueron, Samuel, Boulet, Pascal, Dubowski, Jan J., Elie-Caille, Céline, and Leblois, Thérèse
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- 2020
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4. Molecular and nanoscale evaluation of N-cadherin expression in invasive bladder cancer cells under control conditions or GW501516 exposure
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Elie-Caille, Céline, Lascombe, Isabelle, Péchery, Adeline, Bittard, Hugues, and Fauconnet, Sylvie
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- 2020
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5. NanoBioAnalytical characterization of extracellular vesicles in 75-nm nanofiltered human plasma for transfusion: A tool to improve transfusion safety
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Obeid, Sameh, Sung, Pei-Shan, Le Roy, Benoit, Chou, Ming-Li, Hsieh, Shie-Liang, Elie-Caille, Celine, Burnouf, Thierry, and Boireau, Wilfrid
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- 2019
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6. Gold/Silica biochips: applications to Surface Plasmon Resonance and fluorescence quenching
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Mangeat, Thomas, Berthier, Alexandre, Elie-Caille, Céline, Perrin, Maud, Boireau, Wilfrid, Pieralli, Christian, and Wacogne, Bruno
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Physics - Optics ,Condensed Matter - Materials Science ,Physics - Instrumentation and Detectors - Abstract
We report Gold/Silica biochips for low cost biosensor devices. Firstly, the study of biochemical interactions on silica by means of Surface Plasmon Resonance (SPR) is presented. Secondly, Gold/Silica biochips are employed to reduce the strong quenching that occurs when a fluorophore is close to the gold surface. Furthermore, the control of the Silica-like thickness allows optimizing the distance between the metallic surface and the fluorophore in order to enhance the fluorescent signal. These results represent the first steps towards highly sensitive, specific and low cost biosensors based, for example, on Surface Plasmon Coupled Emission (SPCE) techniques.
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- 2010
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7. Development of a NanoBioAnalytical platform for "on-chip" qualification and quantification of platelet-derived microparticles
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Obeid, Sameh, Ceroi, Adam, Mourey, Guillaume, Saas, Philippe, Elie-Caille, Celine, and Boireau, Wilfrid
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- 2017
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8. Enhanced chemiluminescence-based detection on gold substrate after electrografting of diazonium precursor-coated gold nanoparticles
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Houmed Adabo, Ali, Zeggari, Rabah, Mohamed Saïd, Nasser, Bazzi, Rana, Elie-Caille, Céline, Marquette, Christophe, Martini, Matteo, Tillement, Olivier, Perriat, Pascal, Chaix, Carole, Boireau, Wilfrid, and Roux, Stéphane
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- 2016
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9. Microfluidics for High Pressure: Integration on GaAs Acoustic Biosensors with a Leakage-Free PDMS Based on Bonding Technology
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Hammami, Saber, primary, Oseev, Aleksandr, additional, Bargiel, Sylwester, additional, Zeggari, Rabah, additional, Elie-Caille, Céline, additional, and Leblois, Thérèse, additional
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- 2022
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10. Les vésicules extracellulaires
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Boireau, Wilfrid, primary and Elie-Caille, Céline, additional
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- 2021
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11. Development of extracellular vesicle-based medicinal products: A position paper of the group “Extracellular Vesicle translatiOn to clinicaL perspectiVEs – EVOLVE France”
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Silva, Amanda K.A., primary, Morille, Marie, additional, Piffoux, Max, additional, Arumugam, Surendar, additional, Mauduit, Phlippe, additional, Larghero, Jérôme, additional, Bianchi, Arnaud, additional, Aubertin, Kelly, additional, Blanc-Brude, Olivier, additional, Noël, Danièle, additional, Velot, Emilie, additional, Ravel, Célia, additional, Elie-Caille, Céline, additional, Sebbagh, Anna, additional, Boulanger, Chantal, additional, Wilhelm, Claire, additional, Rahmi, Gabriel, additional, Raymond-Letron, Isabelle, additional, Cherukula, Kondareddy, additional, Montier, Tristan, additional, Martinaud, Christophe, additional, Bach, Jean-Marie, additional, Favre-Bulle, Olivier, additional, Spadavecchia, Jolanda, additional, Jorgensen, Christian, additional, Menasché, Philippe, additional, Aussel, Clotilde, additional, Chopineau, Joël, additional, Mosser, Mathilde, additional, Ullah, Matti, additional, Sailliet, Nicolas, additional, Luciani, Nathalie, additional, Mathieu, Noëlle, additional, Rautou, Pierre-Emmanuel, additional, Brouard, Sophie, additional, Boireau, Wilfrid, additional, Jauliac, Sébastien, additional, Dedier, Marianne, additional, Trouvin, Jean-Hugues, additional, Gazeau, Florence, additional, Trouillas, Marina, additional, Peltzer, Juliette, additional, Monsel, Antoine, additional, and Banzet, Sébastien, additional
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- 2021
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12. Regenerable ZnO/GaAs Bulk AcousticWave Biosensor for Detection of Escherichia coli in 'Complex' Biological Medium
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Chawich, Juliana, Hassen, Walid, Elie-Caille, Céline, Leblois, Thérèse, Dubowski, Jan J, Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] (3IT), Université de Sherbrooke (UdeS), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)
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[SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph] ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,[SPI.MAT]Engineering Sciences [physics]/Materials - Abstract
International audience; A regenerable bulk acoustic wave (BAW) biosensor is developed for the rapid, label-free and selective detection of
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- 2021
13. Regenerable ZnO/GaAs Bulk Acoustic Wave Biosensor for Detection of Escherichia coli in “Complex” Biological Medium
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Chawich, Juliana, primary, Hassen, Walid M., additional, Elie-Caille, Céline, additional, Leblois, Thérèse, additional, and Dubowski, Jan J., additional
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- 2021
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14. Continuous sorting of submicron particles in a pre-analytical device based on acousto-fluidic microsystem
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Chaalane, Amar, Guneysu, Daniel, Addouche, Mahmoud, Zeggari, Rabah, Lardet-Vieudrin, Franck, Elie-Caille, Céline, Boireau, Wilfrid, Khelif, Abdelkrim, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)
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[SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph] ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,[SPI.MAT]Engineering Sciences [physics]/Materials - Abstract
International audience; In this work, we present a pre-analytical module based on the combination of microfluidic and electroacoustic technologies to intercept and sort submicron biological particles. It is composed by assembling a lithium niobate (LN) substrate for acoustic function and a micromachined glass substrate for microfluidic channel. The interference between the two surface acoustic waves (SAWs) generated by inter digital transducers (IDTs) create a distribution of an acoustic radiation force (ARF). This force affects differently particles depending on their physical proprieties. The device is powered by an electronic circuit with a phase shifter to move the node of the standing surface acoustic wave (SSAW) along the channel width. When the device is powered at resonance frequency, experience has shown a submicron particles alignement at a fixed position along the channel. By shifting the SSAW phase, the particles are driven to one of the three channel outlets.
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- 2019
15. Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor
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Oseev, Aleksandr, primary, Mukhin, Nikolay, additional, Elie-Caille, Céline, additional, Boireau, Wilfrid, additional, Lucklum, Ralf, additional, Lecompte, Thomas, additional, Remy-Martin, Fabien, additional, Manceau, Jean-François, additional, Chollet, Franck, additional, and Leblois, Thérèse, additional
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- 2020
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16. Les vésicules extracellulaires: Définition, séparation, caractérisation.
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Boireau, Wilfrid and Elie-Caille, Céline
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- 2021
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17. The nanobioanalytical platform, a tuneable tool for a sensitive detection & characterization of extracellular vesicles subsets from biological samples
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Namasivayam, Balasubramaniam, Wu, Yu-Wen, Delila, Liling, Frelet Barrand, Annie, Burnouf, Thierry, Elie-Caille, Céline, Boireau, Wilfrid, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), and Taipei Medical University
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[SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph] ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,[SPI.MAT]Engineering Sciences [physics]/Materials - Abstract
International audience; Introduction: The NanoBioAnalytical (NBA) platform is an established, calibrated and label-free system to characterize Extracellular Vesicles (EVs), without limitation in size, in different biological samples [1, 2]. NBA benefits were recently highlighted in latest MISEV guidelines [3]. The NBA platform combines biodetection and phenotyping of EVs subsets by immunocapture monitored by Surface Plasmon Resonance (SPR) on biochip, followed by EVs quantitation and sizing thanks to metrological evaluation by Atomic Force Microscopy (AFM). Our aim is to push the limit of the NBA to address clinical studies involving EVs.Methods: We emphasise here the performance of the NBA platform for establishing its dynamic range and limit of detection (LOD) for blood derived EVs. Concentration of EVs was first determined in solution by Tunable Resistive Pulse Sensing; NBA sensitivity and reliability was then studied by SPR on biochips presenting a-CD41 antibody arrays. Finally, even on 1000-fold diluted samples, reliable and complementary information to SPR measurements on size distribution, counting and shape deciphering could be obtained by AFM.Results: Optimizing different factors (flow rate, density of receptors on the surface, etc.) enabled detection of blood derived EVs at dynamic range from 106 to 109 particles /mL on a-CD41 surface. The determination of the LOD of EVs and their subsets size distribution at different capture levels are currently in progress.Summary/Conclusion: The NBA platform is modular and capable of detecting EVs reliably even in highly diluted samples. Such characterization and correlation studies are crucial for accurate and comprehensive characterization of EVs in biological samples with good reproducibility.
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- 2019
18. Tunable Separation of Nanoparticles in a Continuous Flow Using Standing Surface Acoustic Wave
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Chaalane, Amar, Addouche, Mahmoud, Zeggari, Rabah, Guneysu, Daniel, Lardet-Vieudrin, Franck, Bermak, Amine, Elie-Caille, Céline, Boireau, Wilfrid, Khelif, Abdelkrim, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), and Khalifa University (Khalifa University)
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[SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph] ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,[SPI.MAT]Engineering Sciences [physics]/Materials - Abstract
International audience; Manipulating micro and nano-biological particles like extracellular vehicles (EVs), without extracting them from their biological media, presents a big challenge for diagnosis purposes. Here we present the design and fabrication of a sorting device based on the combination of microfluidic and electroacoustic modules that is capable of aligning and sorting submicron biological particles according to their size, compressibility or mass density, all in a tunable way. The device relies on a lithium niobate (LN) substrate to generate acoustic waves assembled with a micromachined glass layer for microfluidic circuits. The interference between the two surface acoustic waves (SAWs) generated by interdigitated transducers (IDTs) create a distribution of an acoustic radiation force (ARF). This force affects differently particles depending on their physical proprieties. The device is powered by an electronic circuit with a phase shifter to move the node of the standing surface acoustic wave (SSAW) along the channel width. When the device is powered at resonance frequency of the IDTs, experiment shows submicron particles alignment along the channel. By shifting the electrical signal between the two IDTs we can translate the pressure node at any targeted position in the channel width. The particles are then driven to one selected outlet.
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- 2019
19. A generic Microfluidic Approach for Deciphering Nanoscale biovesiclES propertieS
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Pillemont, Lyne, Guneysu, Daniel, Elie-Caille, Céline, Boireau, Wilfrid, Gué, Anne Marie, PILLEMONT, Lyne, Une approche microfluidique générique pour la qualification des nanoparticules biologiques - - MADNESS2017 - ANR-17-CE09-0025 - AAPG2017 - VALID, Équipe Micro-Nanofluidique pour les sciences de la vie et de l’environnement (LAAS-MILE), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), ANR-17-CE09-0025,MADNESS,Une approche microfluidique générique pour la qualification des nanoparticules biologiques(2017), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)
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[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,[SPI.MECA.MEFL] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Fluids mechanics [physics.class-ph] ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,[SPI.MECA.MEFL]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Fluids mechanics [physics.class-ph] ,[SDV.BIO] Life Sciences [q-bio]/Biotechnology - Abstract
International audience; Extracellular vesicles (EVs) are vesicles shed by almost all cells with nanometric size and heterogeneous characteristics (biogenesis, shape…). EVs allow cell-to-cell communication and have a key role in lot of (patho)physiological reactions1. Since EVs cover a large size range (from 30 to 1000 nm) and originate from every cells, their characterization in their entirety represents a real challenge. Indeed, there is a lack of method enabling their isolation and analysis, in complex media, that impairs EVs to be qualified and used as biomarkers. Succeeding in EVs detection from their physiological environment and analyzing them in different subsets represent ambitious objective of many research groups. To reach this goal, one solution is to combine several technics and/or improve technical and preanalytical steps. We propose here to develop a microfluidic device, based on hydrodynamic filtration, also called deterministic lateral displacement : the aim of this system is to separate EVs to desired size if EVs are between sidewall and the cutoff radius (Rc), which are the maximum radius value allowing particle to pass in the lateral channel. A fluid flow, containing particles, is introduced into the main channel. In the cross section, only small portion of fluid pass in lateral channel: particles with radius smaller than Rc. This system will be coupled to a home-made gold biochip presenting microarrays enabling specific EVs subsets capture. Then, this lab-on-chip would make possible the recovery and analysis of EVs subsets at once.
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- 2018
20. Plateforme NanoBioAnalytique pour la caractérisation des vésicules extracellulaires
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Elie-Caille, Céline, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)
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[SPI.ACOU]Engineering Sciences [physics]/Acoustics [physics.class-ph] ,[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics ,[SPI.MAT]Engineering Sciences [physics]/Materials - Abstract
International audience; Workshop « À la conquête des nanovésicules biologiques »
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- 2017
21. Formation Kinetics of Mixed Self-Assembled Monolayers of Alkanethiols on GaAs(100)
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Lacour, Vivien, primary, Moumanis, Khalid, additional, Hassen, Walid M., additional, Elie-Caille, Céline, additional, Leblois, Thérèse, additional, and Dubowski, Jan J., additional
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- 2017
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22. Cell imaging connected with protein mapping to investigate glyphosate induced toxicity on human keratinocytes
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Elie-Caille, Céline, Heu, Celine, Ewald, Maxime, Lesniewska, E., Nicod, L., Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Ingénierie et biologie cellulaire et tissulaire (IBCT (ex IFR133)), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Laboratoire Interdisciplinaire Carnot de Bourgogne (LICB), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies ( FEMTO-ST ), Université de Technologie de Belfort-Montbeliard ( UTBM ) -Ecole Nationale Supérieure de Mécanique et des Microtechniques ( ENSMM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Ingénierie et biologie cellulaire et tissulaire ( IBCT (ex IFR133) ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Laboratoire Interdisciplinaire Carnot de Bourgogne ( LICB ), Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), and Elie-Caille, Céline
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[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,[ SDV.BBM.BP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,atomic force microscopy ,proteomics ,glyphosate ,HaCaT ,[SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics - Abstract
During the skin ageing which is accelerated by environmental factors, the generated reactive oxygen species make unstable the "proliferation/differentiation/apoptosis" coupling of epidermal cells. A biochemical study, previously realized on human keratinocyte cultures treated by a pesticide, glyphosate, allowed the validation of an in vitro model of skin ageing. In this work, we propose to study the loss of HaCaT cell integrity appearing after this chemical induced-oxidative stress. An original approach, combining a micro- to nanoscale cell characterization and analysis of fluctuating protein expression, is proposed: a) the exploration of cell growth and morphology through fluorescence and AFM imaging, b) a functional investigation using force spectroscopy for detection of oxidative stress biomarkers in membranes and c) the establishment of proteomic comparative profiles between safe and glyphosate-treated cells. Our results revealed up to now that a glyphosate treatment induced cell impairment in an acute dose-dependency. Indeed, we observed a breakage of plasma membrane and a release of cytosol, microtubules disorganization and fragmentation, and finally aggregation of chromatin. These observations characterized apoptotic key events. Our first force spectroscopy results revealed that a glyphosate coated tip interacted specifically with keratinocyte membrane structures. This nanobiotechnological tool will allow cell mapping establishment and screening original anti-oxidant molecules. The proteomic profiling would highlight cell oxidative stress cascade and identify corresponding major biomarkers in our skin ageing model. Original molecules with cytoprotective potentials will be tested and compared to well-known antioxidants, in order to correct pesticide induced cutaneous disorders. These data all together will contribute to better encircle the mechanisms of chemical and environmental hazards acting on the human skin.
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- 2009
23. New technologies getting informations on repartition, orientation and identification of biomolecules on SPR chip
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Elie-Caille , Céline, Rouleau , Alain, Lucchi , Géraldine, Aoyagi , Satoka, Ducoroy , Patrick, Boireau , Wilfrid, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies ( FEMTO-ST ), Université de Technologie de Belfort-Montbeliard ( UTBM ) -Ecole Nationale Supérieure de Mécanique et des Microtechniques ( ENSMM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Santé - STIC, Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Faculty of Life and Environmental Science, Université, Elie-Caille, Céline, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), and Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)
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[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,[ SDV.BBM.BP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,TOF-SIMS ,XPS ,[SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,biomarker ,AFM ,Biochip - Abstract
We present herein an original approach, consisting to lead in parallel a global analysis (SPR) and nanoscale characterizations (AFM, mass spectrometry, TOF-SIMS) of bio-molecules on the biochip surface. The questions we want to answer concern especially the organization, orientation and identification of immobilized and captured biomolecules. In order to characterize the sensing layer in terms of molecular structure and orientation, we engaged a SPR-TOF SIMS first set of experiments (collaboration: Pr Aoyagi, Japan). Our results demonstrated that analysis of orientation of the immobilized cytochrome b5 is possible (ref2&3) and help to optimize and develop our sensing layer. We also recently developed an innovative concept to identify directly « on the chip » (without any elution) the biomolecules retained by mass spectrometry. We first validated the concept on a simple model (monolayer of albumin) with sensitivity for MS identification less than 1 fmol/mm2 (ref1). We further continued by developing immunosensors to catch specifically, in complex biological fluids like sera, low concentrated biomarkers (lag3 protein). Controlling the surface coverage and repartition of molecules by AFM, mass spectrometry “on the chip” allowed to identify without ambiguity both antibody and biomarker. Our objective is now to increase the throughput of analysis. For that, we are currently working on micro-arrays. Our expertise in molecule grafting and nanocharacterization will make our clinical proteomic research relevant, thanks to deeply controlled steps of biosensor development and use. References (1) Lucchi G., Rouleau A., Boireau W., Ducoroy P. SMAP 2007 (Congress on Mass Spectrometry and Proteomics Analysis), Pau (France). (2) S. Aoyagi, W. Boireau, C. Elie-Caille, A. Rouleau, M. Dohi, N. Kato, M. Kudo. 45th Annual Meeting of the Biophysical Society of Japan (Dec 2007), Pacifico Yokohama. (3) S. Aoyagi, A. Rouleau, W. Boireau. Applied Surface Science, (in press).
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- 2008
24. ERα dimerization: a key factor for the weak estrogenic activity of an ERα modulator unable to compete with estradiol in binding assays
- Author
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Leclercq, Guy, primary, Laïos, Ioanna, additional, Elie-Caille, Céline, additional, Leiber, Denis, additional, Laurent, Guy, additional, Lesniewska, Eric, additional, Tanfin, Zahra, additional, and Jacquot, Yves, additional
- Published
- 2016
- Full Text
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25. Regeneration of a thiolated and antibody functionalized GaAs (001) surface using wet chemical processes
- Author
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Lacour, Vivien, primary, Elie-Caille, Céline, additional, Leblois, Thérèse, additional, and Dubowski, Jan J., additional
- Published
- 2016
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26. Formation Kinetics of Mixed Self-Assembled Monolayers of Alkanethiols on GaAs(100)
- Author
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Lacour, Vivien, Moumanis, Khalid, Hassen, Walid M., Elie-Caille, Céline, Leblois, Thérèse, and Dubowski, Jan J.
- Abstract
We report on the formation kinetics of mixed self-assembled monolayers (SAMs) comprising 16-mercaptohexadecanoic acid (MHDA) and 11-mercapto-1-undecanol (MUDO) thiols on GaAs(100) substrates. These compounds were selected for their potential in constructing highly selective and efficient architectures for biosensing applications. The molecular composition and quality of one-compound and mixed SAMs were determined by the Fourier transform infrared absorption spectroscopy measurements. The formation of enhanced-quality mixed SAMs was investigated as a function of the molecular composition of the thiol mixture and the proportion of ethanol/water solvent used during their arrangement. Furthermore, the formation of mixed SAMs has been carried out by successive immersion of MHDA SAMs in MUDO thiol solutions and MUDO SAMs in MHDA thiol solution through the process involving thiol–thiol substitution. Our results, in addition to confirming that water–ethanol-based solvents improve the packing density of single thiol monolayers, demonstrate the attractive role of water–ethanol solvents in forming superior quality mixed SAMs.
- Published
- 2019
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27. Conformational adaptability of RedB during DNA annealing and implications for its structural relationship with Rad52
- Author
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Erler, Axel, Susanne, Wegmann, Elie-Caille, Céline, Bradshaw, Cr, Maresca, Marcello, Seidel, Ralf, Habermann, B., J. Muller, Daniel, Stewart, Francis, Genomics, Biotec, Dresden, Department of Cellular Machines, BioTechnological Center, Technische Universität Dresden = Dresden University of Technology (TU Dresden), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)
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recombineering ,[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,atomic force microscopy ,viruses ,homologous recombination ,phage lambda ,DNA annealing - Abstract
International audience; Single-strand annealing proteins, such as Redbeta from lambda phage or eukaryotic Rad52, play roles in homologous recombination. Here, we use atomic force microscopy to examine Redbeta quaternary structure and Redbeta-DNA complexes. In the absence of DNA, Redbeta forms a shallow right-handed helix. The presence of single-stranded DNA (ssDNA) disrupts this structure. Upon addition of a second complementary ssDNA, annealing generates a left-handed helix that incorporates 14 Redbeta monomers per helical turn, with each Redbeta monomer annealing approximately 11 bp of DNA. The smallest stable annealing intermediate requires 20 bp DNA and two Redbeta monomers. Hence, we propose that Redbeta promotes base pairing by first increasing the number of transient interactions between ssDNAs. Then, annealing is promoted by the binding of a second Redbeta monomer, which nucleates the formation of a stable annealing intermediate. Using threading, we identify sequence similarities between the RecT/Redbeta and the Rad52 families, which strengthens previous suggestions, based on similarities of their quaternary structures, that they share a common mode of action. Hence, our findings have implications for a common mechanism of DNA annealing mediated by single-strand annealing proteins including Rad52.
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- 2009
28. Nanobiologie : la révolution diagnostique
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Elie-Caille, Céline, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)
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[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,biopuce ,diagnostique ,Nanooutils - Abstract
National audience; De la nanotechnologie ... Les nanotechnologies recouvrent l'ensemble des techniques, outils et procédés qui permettent de manipuler la matière à une échelle en dessous de la centaine de nanomètres (1 nanomètre = 1 milliardième de mètre), d'élaborer de nouveaux matériaux et composants toujours plus petits, de construire atome par atome de nouvelles molécules et d'exploiter leurs propriétés en vue de nouvelles applications. Les nanotechnologies devraient constituer dans les prochaines années un marché considérable. À l'horizon 2015, 15% de l'activité manufacturière mondiale serait concernée par des dispositifs ou des matériaux utilisant des avancées issues des nanotechnologies. En 2008, le montant du marché mondial est estimé à 500 milliards de dollars et pourrait doubler en 2015, selon la National science foundation. Toutefois, les nanotechnologies suscitent également des inquiétudes. Manipuler la matière à l'échelle moléculaire et interférer avec le monde du vivant soulève des questions éthiques et sanitaires qui doivent être prises en considération par les pouvoirs publics et les acteurs concernés. La nanotechnologie peut être considérée comme une évolution logique de la microélectronique dont l'objectif est la fabrication de circuits intégrés, familièrement appelés « puces électroniques » dont la référence en matériau de base est le silicium. A côté de la nanoélectronique industrielle a émergé la nanotechnologie moléculaire. Le but de cette dernière discipline est de pouvoir assurer un contrôle parfait sur la structure de la matière et de pouvoir construire des objets complexes avec une précision à l'échelle de l'atome ou de la molécule. ... aux nanoBIOtechnologies... Un important challenge dans les dispositifs de BIOcapteurs et de diagnostic est de savoir comment reconstituer/étudier des mécanismes biologiques pertinents à la surface d'une biopuce et quels outils analytiques sont convenables pour fournir rapidement des informations justes sur la structure des molécules attachées à la surface. Un meilleur contrôle dans la réalisation de biopuces peut être obtenu en combinant différentes approches interdisciplinaires, avec pour préoccupation de répondre à une question clé biologique. Les recherches menées au sein de la plateforme CLIPP (Clinical & Innovation Proteomic Platform), rassemblant des compétences multidisciplinaires, sont focalisées sur cet objectif. L'ensemble « conception, réalisation et caractérisation » de puces à protéines représente un des axes de recherche de la plateforme CLIPP. Nous développons différentes stratégies d'ingénierie/structuration de matériaux, de fonctionnalisation chimique et d'immobilisation de biomolécules qui conduit à l'optimisation d'évènements de reconnaissance et leur caractérisation à la surface de la puce. Dernièrement, nous avons développé une méthode simple pour la préparation de substrat d'or présentant des terrasses atomiquement planes, et permettant une caractérisation biomoléculaire à l'échelle nanométrique1. Le mode d'action du taxol sur les microtubules et les conséquences sur leur conformation a pu également être élucidé par observation par microscopie à force atomique (AFM) en conditions physiologiques2. Nous avons également développé des approches originales, consistant à combiner une analyse « globale » par Surface Plasmon Resonance (SPR) et un moyen de caractérisation à l'échelle nanométrique (AFM, Spectroscopie de Photo-électrons X, spectrométrie de masse (MS)). Nous avons en particulier démontré que l'orientation d'une protéine immobilisée en monocouche sur une surface, peut être déterminée par la détection de fragments secondaires spécifiques de la protéine par TOF-SIMS3. Ces développements ont contribué à l'établissement d'une nouvelle combinaison « Analyse des interactions Biomoléculaires / spectrométrie de masse (BIA-MS) basée sur une procédure totalement « on the chip »4. Nous proposons une approche bas coût combinant l'analyse sur un BIAcore 2000 sur puces synthétisées par nos soins (réalisation du substrat et greffage d'anticorps) avec l'identification par MS, directement sur la puce et sans étape d'élution. Utilisant cette technique, l'identification de complexes protéiques a pu être obtenue, et permet ainsi de compléter l'approche BIA-MS dans la découverte et l'analyse de complexes protéiques. Dernièrement, ces développements ont été transposés à de l'analyse multiplexée sur micro-arrays. Les premiers résultats de détection de biomarqueurs dans du plasma s'avèrent être très prometteurs pour une approche diagnostic moyen/haut débit.
- Published
- 2009
29. Morphological and functional characterization of the consequences of the oxidative stress experimentally induced on skin cell models in vitro
- Author
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Elie-Caille, Céline, Akkus, Bektas, Nicod, Laurence, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Ingénierie et biologie cellulaire et tissulaire (IBCT (ex IFR133)), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), IFR133, and Besancon
- Subjects
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,Oxidative stress ,morphology ,AFM ,skin cells - Abstract
During the skin ageing, the produced radical species make unstable the "proliferation / differentiation / apoptosis" coupling of epidermal cells; we propose to study this loss of cell integrity, by a double original and complementary approach: - the establishment of proteomic comparative profiles, by spectrofluorimetric, 2D chromatographic/mass spectrometry techniques, - the exploration of cell growth and morphology, by a spectroscopic and topographic study by atomic force microscopy. This work will allow identifying the better biomarker(s) of skin ageing modeled in vitro by a chosen chemical treatment using glyphosate. Original molecules with cell protection effect, able to correct the induced morphological and/or functional disorders could also been identified. Our first results revealed cell morphology of a line of keratinocytes grown on different substrates. A glyphosate dose-effect was noted through an important cell destructuration. Preliminary force spectroscopy measurements on cells revealed also specific interactions with glyphosate. These experimental results based on AFM investigation should highlight the first steps of the oxidative stress mechanism, by visualization and quantification of the phenomenon after its induction at the individual cell scale.
- Published
- 2008
30. New Insights into Redβ-mediated DNA Annealing using Atomic Force Microscopy
- Author
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Erler, Axel, Susanne, Wegmann, Elie-Caille, Céline, Maresca, Marcello, Seidel, Ralf, Heine, Tobias, Daniel, Muller, Stewart, Francis, Department of Cellular Machines, BioTechnological Center, Technische Universität Dresden = Dresden University of Technology (TU Dresden), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), and Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,atomic force microscopy ,DNA annealing ,redB - Abstract
International audience; Redβ anneals DNA to initiate homologous recombination ined recent prominence through the development of the DNA engineering technology known as ‘recombineering' or ‘Red/ET'. It originates from the red-operon of λ phage where it is co-expressed in the early life cycle stage with Redα a processive 5'-3' exonuclease and Redγ, a DNA mimetic and RecBCD inhibitor. Unlike RecA/RAD51, Redβ is not an ATPase and it's mechanism for initiating homologous recombination is poorly understood. To examine the structure and dynamics of Redβ complexes at sub-molecular resolution we performed tapping mode atomic force microscopy (AFM) of Redβ protein alone and in complex with DNA. Without DNA, Redβ forms a ‘split lock washer' structure with a shallow right-handed helicity. Sequentially adding complementary ssDNA generates a stable left-handed helical filament. Importantly, the contour length of the helical filament equated linearly to the lengths of complementary ssDNA, giving the number of nucleotides per Redβ monomer. Additionally, the monomer width along the filament was quantified. These new quantities as well as the observed helical transition reveals new insights into the mechanism of DNA annealing mediated by Red and led us to suggest new mechanistic models.
- Published
- 2008
31. ERα dimerization: a key factor for the weak estrogenic activity of an ERα modulator unable to compete with estradiol in binding assays.
- Author
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Leclercq, Guy, Laïos, Ioanna, Elie-Caille, Céline, Leiber, Denis, Laurent, Guy, Lesniewska, Eric, Tanfin, Zahra, and Jacquot, Yves
- Abstract
Estrothiazine (ESTZ) is a weak estrogen sharing structural similarities with coumestrol. ESTZ failed to compete with [
3 H]17β-estradiol ([3 H]17β-E2 ) for binding to the estrogen receptor α (ERα), questioning its ability to interact with the receptor. However, detection by atomic force spectroscopy (AFS) of an ESTZ-induced ERα dimerization has eliminated any remaining doubts. The effect of the compound on the proliferation of ERα-positive and negative breast cancer cells confirmed the requirement of the receptor. The efficiency of ESTZ in MCF-7 cells was weak without any potency to modify the proliferation profile of estradiol and coumestrol. Growth enhancement was associated with a proteasomal degradation of ERα without substantial recruitment of LxxLL coactivators. This may be related to an unusual delay between the acquisition by the receptor of an ERE-binding capacity and the subsequent estrogen-dependent transcription. A complementary ability to enhance TPA-induced AP-1 transcription was observed, even at concentrations insufficient to activate the ERα, suggesting a partly independent mechanism. ESTZ also rapidly and transiently activated ERK1/2 likely through membrane estrogenic pathways provoking a reorganization of the actin network. Finally, the systematic absence of biological responses with an ESTZ derivative unable to induce ERα dimerization stresses the importance of this step in the action of the compound, as reported for conventional estrogens. In view of the existence of many other ERα modulators (endocrine disruptors such as, for example, pesticides, environmental contaminants or phytoestrogens) with extremely weak or similar apparent lack of binding ability, our work may appear as pilot investigation for assessing their mechanism of action. [ABSTRACT FROM AUTHOR]- Published
- 2017
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- View/download PDF
32. High Sensitive Mass Detection using Piezoelectric Coupled Microcantilevers
- Author
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Chopard, Tony, primary, Bienaime, Alex, additional, Elie-Caille, Céline, additional, and Leblois, Thérèse, additional
- Published
- 2012
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33. Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor
- Author
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Berthier, Alexandre, primary, Elie-Caille, Céline, additional, Lesniewska, Eric, additional, Delage-Mourroux, Régis, additional, and Boireau, Wilfrid, additional
- Published
- 2008
- Full Text
- View/download PDF
34. Straight GDP-Tubulin Protofilaments Form in the Presence of Taxol
- Author
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Elie-Caille, Céline, primary, Severin, Fedor, additional, Helenius, Jonne, additional, Howard, Jonathon, additional, Muller, Daniel J., additional, and Hyman, A.A., additional
- Published
- 2007
- Full Text
- View/download PDF
35. Production of microporous aluminum oxide electrodes as supports for tethered lipid bilayers of large surface area
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Fliniaux, Ophélie, primary, Elie-Caille, Céline, additional, Pantigny, Jacques, additional, and Bourdillon, Christian, additional
- Published
- 2005
- Full Text
- View/download PDF
36. Self-Assembly of Solid-Supported Membranes Using a Triggered Fusion of Phospholipid-Enriched Proteoliposomes Prepared from the Inner Mitochondrial Membrane1
- Author
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Elie-Caille, Céline, primary, Fliniaux, Ophélie, additional, Pantigny, Jacques, additional, Mazière, Jean-Claude, additional, and Bourdillon, Christian, additional
- Published
- 2005
- Full Text
- View/download PDF
37. Technological innovation around protein and cell biochip for diagnosis: a translational research from nanoworld to patient
- Author
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Celine Elie-Caille, Alain Rouleau, Benoit Simon, Céline Heu, Geraldine Lucchi, Wilfrid Boireau, Patrick Ducoroy, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies ( FEMTO-ST ), Université de Technologie de Belfort-Montbeliard ( UTBM ) -Ecole Nationale Supérieure de Mécanique et des Microtechniques ( ENSMM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Plate-forme Protéomique CLIPP - Clinical and Innovation Proteomic Platform [Dijon] ( CLIPP ), Université de Technologie de Belfort-Montbeliard ( UTBM ) -Ecole Nationale Supérieure de Mécanique et des Microtechniques ( ENSMM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ) -Université de Technologie de Belfort-Montbeliard ( UTBM ) -Ecole Nationale Supérieure de Mécanique et des Microtechniques ( ENSMM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ) -Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] ( ICMUB ), Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Elie-Caille, Céline, Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Plate-forme Protéomique CLIPP - Clinical and Innovation Proteomic Platform [Dijon] (CLIPP), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Simon, Benoît
- Subjects
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,[ SDV.BBM.BP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,atomic force microscopy ,nanostructuration ,diagnosis ,biochip ,[SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,surface functionalization ,mass spectrometry - Abstract
International audience; A great challenge in biosensors and diagnosis devices relies on the way to reconstitute relevant biological mechanisms on surface of the biochips and which analytical tools are convenient to provide accurate and rapid information on the structures and function of molecules attached to this surface. A better control in the realization of biochips can be obtained in combining different complementary approaches while always keeping in mind the biological key point. Researches in CLIPP are focused towards this objective. Conception, realization and characterization of protein and cell chips are presented. We detail different strategies of materials engineering1,2,3, chemical functionalizations and biomolecular graftings4, molecular and cellular characterization in physiological conditions5,6, which lead to the optimization of “biorecognitions events” at the surface of the chip. We present herein an original interdisciplinary approach, consisting to carry out in parallel a micro-scale analysis (SPR, fluorescence microscopy) and nano-scale characterizations (AFM, XPS, TOF-SIMS). Concerning protein interfaces, we demonstrated in particular that the molecular orientation in a protein monolayer can be determined based on the specific fragment ions from the protein in TOF-SIMS spectra7. These developments have also contributed to the establishment of a new biomolecular interaction analysis/mass spectrometry (BIA-MS) combination based on an entire “on-a-chip” procedure8. We report a low-cost approach combining Biacore 2000 analysis with homemade chips and MS and MS/MS identification directly onto the chips without elution step. Using this technique, identification of protein complexes were routinely obtained giving the opportunity to the “on-a-chip” processing to complete the BIA-MS approach in the discovery and analysis of protein complexes in biological fluids. Our interest is also focused on cell/surface interaction. The cell biochips we are developing consist either of circulating or adherent cells, that we characterized in terms of cell capture on biofunctionalized surface or growth with substrate dependency respectively. Parameters such as cell spreading, growth, morphology, and topography are particularly investigated and controlled by atomic force microscopy in physiological conditions6. With the aim to increase the throughput of analysis, we are currently working on cell and protein micro-arrays. Our expertise in cell and protein biochip preparation, and competences in micro- to nanoscale characterization in liquid conditions, represents precious assets enabling a relevant clinical proteomic research, thanks to deeply controlled steps of biosensor development and use.
- Published
- 2009
38. Concept of dynamic DNA network dedicated to DNA-Protein interactions studies
- Author
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Elie-Caille, C., Lesniewska, E., Berthier, A., Delage-Mourroux, R., Boireau, W., Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies ( FEMTO-ST ), Université de Technologie de Belfort-Montbeliard ( UTBM ) -Ecole Nationale Supérieure de Mécanique et des Microtechniques ( ENSMM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Laboratoire Interdisciplinaire Carnot de Bourgogne ( LICB ), Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Ingénierie et biologie cellulaire et tissulaire ( IBCT (ex IFR133) ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Etablissement français du sang [Bourgogne-France-Comté] ( EFS [Bourgogne-France-Comté] ) -Université de Franche-Comté ( UFC ), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interdisciplinaire Carnot de Bourgogne (LICB), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Ingénierie et biologie cellulaire et tissulaire (IBCT (ex IFR133)), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), and Elie-Caille, Céline
- Subjects
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,[ SDV.BBM.BP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,tethered lipid membrane ,[SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics ,SPR ,AFM ,DNA biosensor ,estrogen receptor - Abstract
The approach lies on the development of an original DNA biosensor. This self-assembled DNA biochip is constituted of a tethered lipid membrane presenting « protein/DNA » complexes. This unique assembly, using a highly flexible bifunctional molecular spacer and a fluidic matrix, allows the control of oligonucleotides surface density and their mobility thus favouring hybridization of the DNA probes in liquid environment and under weak constraints. Recent Surface Plasmon Resonance experiments lead to building of protein/DNA dimers presenting two special DNA sequences recognized by regulation proteins. We demonstrated the capacity and relevance of this biosensor in the case of an estrogen receptor/DNA specific recognition, opening the possibility of specific drugs screening. We present here preliminary characterizations of these thin biomolecular films through Atomic Force Microscopy in liquid. Homogeneity of the lipid film was controlled, the surface coverage in protein was evaluated and specificity of binding is under examination at the present time. Indeed, the visualization of the self-assembled « edifice » at a molecular scale and in a bio-mimetic environment is a precious and complementary way to improve the understanding of the protein/DNA interaction mechanisms.
- Published
- 2007
39. [Extracellular vesicles: Definition, isolation and characterization].
- Author
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Boireau W and Elie-Caille C
- Subjects
- Eukaryotic Cells, Prokaryotic Cells, Extracellular Vesicles
- Abstract
Extracellular vesicles (EVs) originate from eukaryotic and prokaryotic cells and play a crucial role in intercellular communications. They are found in the environment of cells and tissues, and contribute to the complexity of different biological media, in particular biofluids. Due to their high diversity of cell origin, size range, concentration and composition, EVs offer some of the most important challenges in (pre-)analytical fields. To tackle these challenges, many works deal with the development and implementation of a wide variety of approaches, technologies and methodologies to enrich, isolate, quantify and characterize EVs and their subsets. Nevertheless, other components such as lipoproteins or viruses in complex samples, can interfere with EVs qualification, and make difficult, even today, to standardize biochemical and physical approaches for this purpose. The present chapter presents EVs and the mostly used technics for their isolation and characterization. Performances of methods in terms of resolution, discrimination, throughput and also ability to be or not applied in clinics, are also discussed., (© 2021 médecine/sciences – Inserm.)
- Published
- 2021
- Full Text
- View/download PDF
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