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Migration is energetically expensive and is predicted to drive similar morphological adaptations and physiological trade-offs in migratory bats and birds. Previous studies suggest that fixed traits like wing morphology vary among species and individuals according to selective pressures on flight, while immune defences can vary flexibly within individuals as energy is variably reallocated throughout the year. We assessed intraspecific variation in wing morphology and immune function in silver-haired bats Lasionycteris noctivagans, a species that follows both partial and differential migration patterns. We hypothesized that if bats experience energy constraints associated with migration, then wing morphology and immune function should vary based on migratory tendency (sedentary or migratory) and migration distance. We predicted that long-distance migrants would have reduced immune function and more migration-adapted wing shapes compared to resident or short-distance migrating bats. We estimated breeding latitude of spring migrants using stable hydrogen isotope techniques. Our sample consisted primarily of male bats, which we categorized as residents, long-distance northern migrants, short-distance northern migrants and southern migrants (apparent breeding location south of capture site). Controlling for individual condition and capture date, we related wing characteristics and immune indices among groups. Some, but not all, aspects of wing form and immune function varied between migrants and residents. Long-distance northern migrants had larger wings than short-distance northern migrants and lower wing loading than southern migrants. Compared with resident bats, short-distance northern migrants had reduced IgG while southern migrants had heightened neutrophils and neutrophil-to-lymphocyte ratios. Body fat, aspect ratio, wing tip shape and bacteria killing ability did not vary with migration status or distance. In general, male silver-haired bats do not appear to mediate migration costs by substantially downregulating immune defences or to be under stronger selection for wing forms adapted for fast, energy-efficient flight. Such phenotypic changes may be more adaptive for female silver-haired bats, which migrate farther and are more constrained by time in spring than males. Adaptations for aerial hawking and the use of heterothermy by migrating bats may also reduce the energetic cost of migration and the need for more substantial morphological and physiological trade-offs.

Optimal migration theory is a framework used to evaluate trade-offs associated with migratory strategies. Two strategies frequently considered by migration theory are time minimizing, whereby migration is completed as quickly as possible, and energy minimizing, whereby migration is completed as energetically efficiently as possible. Despite extensive literature dedicated to generating analytical predictions about these migratory strategies, identifying appropriate study systems to empirically test predictions is difficult. Theoretical predictions that compare migratory strategies are qualitative, and empirical tests require that both time-minimizers and energy-minimizers are present in the same population; spring migrating silver-haired (Lasionycteris noctivagans) and hoary bats (Lasiurus cinereus) provide such a system. As both species mate in the fall, spring-migrating males are thought to be energy-minimizers while females benefit from early arrival to summering grounds, and are thought to be time-minimizers. Thermoregulatory expression also varies between species during spring migration, as female silver-haired bats and males of both species use torpor while female hoary bats, which implant embryos earlier, are thought to avoid torpor use which would delay pregnancy. Based on optimal migration theory, we predicted that female silver-haired bats and hoary bats would have increased fuel loads relative to males and the difference between fuel loads of male and female hoary bats would be greater than the difference between male and female silver-haired bats. We also predicted that females of both species would have a greater stopover foraging proclivity and/or assimilate nutrients at a greater rate than males. We then empirically tested our predictions using quantitative magnetic resonance to measure fuel load, δ13C isotope breath signature analysis to assess foraging, and13C–labeled glycine to provide an indicator of nutrient assimilation rate. Optimal migration theory predictions of fuel load were supported, but field observations did not support the predicted refueling mechanisms, and alternatively suggested a reliance on increased fuel loads via carry-over effects. This research is the first to validate a migration theory prediction in a system of both time and energy minimizers and uses novel methodological approaches to uncover underlying mechanisms of migratory stopover use.

Much research has sought to understand factors that predict both positive and negative posttrauma outcomes. Social support is one factor that has been widely investigated, primarily as a buffer against the negative effects of stressful life events. While receiving social support has claimed much empirical attention attesting to its positive impact on psychological outcomes following exposure to trauma, the influence of giving social support has received less empirical attention. The current study aimed to assess the influence of giving as well as receiving social support on psychological outcomes in two trauma-exposed samples: flood survivors (N = 351) and firefighters (N = 337), controlling for perceptions of trauma severity. The study used the Brief 2-Way SSS, a measure of both receiving and giving, emotional and instrumental support. Results indicated that although giving social support showed a significant bivariate relationship with depression and stress in the firefighter sample, the unique contribution of giving social support was found only for posttraumatic growth. In contrast, the influence of social support on measures of depression, anxiety, and stress was seen through the receipt of social support. These findings provide support for the value of examining both receiving and giving social support, as they may have differential impacts in buffering against the impact of trauma exposure on psychological outcomes.

As small, flying, mammalian endotherms, insectivorous bats are adapted to operate at high levels of energy expenditure. In response to seasonally variable challenges, we predicted that bats should balance energy budgets by flexibly adjusting aspects of their physiology or behavior in ways that elevate metabolic capacity. We examined variation in energy storage and pathways for oxidative metabolism in Brazilian free-tailed bats (Tadarida brasiliensis) related to estimated costs associated with reproduction and migration. We collected pectoral muscle and liver from female T. brasiliensis at six time points during the summer and fall and measured changes in the activity of four enzymes involved with lipid metabolism. Body mass varied substantially with life-cycle stage, suggesting that rapid accumulation and use of fat stores occurs in response to current and anticipated energy demands. Catabolic enzyme activity (carnitine palmitoyl transferase [CPT], 3-hydroxyacyl-CoA dehydrogenase [HOAD], and citrate synthase [CS]) in the muscle was increased during lactation compared with early pregnancy but exhibited no change before fall migration. While there was no temporal change in lipid biosynthetic capacity in the liver, fatty acid synthase activity was negatively correlated with body mass. Variation in body mass and enzyme activity in T. brasiliensis during the summer suggests that stored energy is mobilized and lipid oxidative capacity is increased during periods of increased demand and that lipid biosynthetic capacity is increased with depletion of fat stores. These results suggest that bats are able to flexibly adjust metabolic capacity based on energy requirement to maintain energy balance despite high levels of expenditure.

As organisms face variation in energetic challenges and physiological demands, they often respond with reversible changes in behavior, physiology, and morphology, described as phenotypic flexibility. From the magnitude of phenotypic change, we can infer the energetic challenges of different life stages. We studied phenotypic flexibility in a population of reproductive and pre-migratory female insectivorous bats (Tadarida brasiliensis). While female reproductive demands are well described in insectivorous bats, there are questions regarding the demands of migration. Our objective was to measure phenotypic flexibility to assess the cost of autumn migration compared to reproduction in an insectivorous bat. We measured plasma triglycerides to quantify foraging rate, and body composition (body mass and individual organ mass) of T. brasiliensis throughout the summer season (from arrival in spring through pre-migration/migration departure in autumn) according to the female reproductive cycle. We found phenotypic changes during pre-migration/migration similar to periods of high-energy demand during reproduction (e.g., late pregnancy and lactation). Most notably, bats weighed as much during peak pregnancy, as they did during migration, and the rapid mass gain from post-lactation through the migratory period was due to a combination of hyperphagia and hypertrophy of digestive organs. Our results indicate that energetic demands incurred during migration are similar to those during reproduction and emphasize the energetic challenges of migration.

The fidelity of aminoacyl-tRNA selection by the ribosome depends on a conformational switch in the decoding center of the small ribosomal subunit induced by cognate but not by near-cognate aminoacyl-tRNA. The aminoglycosides paromomycin and streptomycin bind to the decoding center and induce related structural rearrangements that explain their observed effects on miscoding. Structural and biochemical studies have identified ribosomal protein S12 (as well as specific nucleotides in 16S ribosomal RNA) as a critical molecular contributor in distinguishing between cognate and near-cognate tRNA species as well as in promoting more global rearrangements in the small subunit, referred to as “closure.” Here we use a mutational approach to define contributions made by two highly conserved loops in S12 to the process of tRNA selection. Most S12 variant ribosomes tested display increased levels of fidelity (a “restrictive” phenotype). Interestingly, several variants, K42A and R53A, were substantially resistant to the miscoding effects of paromomycin. Further characterization of the compromised paromomycin response identified a probable second, fidelity-modulating binding site for paromomycin in the 16S ribosomal RNA that facilitates closure of the small subunit and compensates for defects associated with the S12 mutations.

White-nose syndrome (WNS) in North American bats is caused by an invasive cutaneous infection by the psychrophilic fungus Pseudogymnoascus destructans (Pd). We compared transcriptome-wide changes in gene expression using RNA-Seq on wing skin tissue from hibernating little brown myotis (Myotis lucifugus) with WNS to bats without Pd exposure. We found that WNS caused significant changes in gene expression in hibernating bats including pathways involved in inflammation, wound healing, and metabolism. Local acute inflammatory responses were initiated by fungal invasion. Gene expression was increased for inflammatory cytokines, including interleukins (IL) IL-1β, IL-6, IL-17C, IL-20, IL-23A, IL-24, and G-CSF and chemokines, such as Ccl2 and Ccl20. This pattern of gene expression changes demonstrates that WNS is accompanied by an innate anti-fungal host response similar to that caused by cutaneous Candida albicans infections. However, despite the apparent production of appropriate chemokines, immune cells such as neutrophils and T cells do not appear to be recruited. We observed upregulation of acute inflammatory genes, including prostaglandin G/H synthase 2 (cyclooxygenase-2), that generate eicosanoids and other nociception mediators. We also observed differences in Pd gene expression that suggest host-pathogen interactions that might determine WNS progression. We identified several classes of potential virulence factors that are expressed in Pd during WNS, including secreted proteases that may mediate tissue invasion. These results demonstrate that hibernation does not prevent a local inflammatory response to Pd infection but that recruitment of leukocytes to the site of infection does not occur. The putative virulence factors may provide novel targets for treatment or prevention of WNS. These observations support a dual role for inflammation during WNS; inflammatory responses provide protection but excessive inflammation may contribute to mortality, either by affecting torpor behavior or causing damage upon emergence in the spring., Author Summary White-nose syndrome is the most devastating epizootic wildlife disease of mammals in history, having killed millions of hibernating bats in North America since 2007. We have used next-generation RNA sequencing to provide a survey of the gene expression changes that accompany this disease in the skin of bats infected with the causative fungus. We identified possible new mechanisms that may either provide protection or contribute to mortality, including inflammatory immune responses. Contrary to expectations that hibernation represents a period of dormancy, we found that gene expression pathways were responsive to the environment. We also examined which genes were expressed in the pathogen and identified several classes of genes that could contribute to the virulence of this disease. Gene expression changes in the host were associated with local inflammation despite the fact that the bats were hibernating. However, we found that hibernating bats with white-nose syndrome lack some of the responses known to defend other mammals from fungal infection. We propose that bats could be protected from white-nose syndrome if these responses could be established prior to hibernation or if treatments could block the virulence factors expressed by the pathogen.

White-nose syndrome (WNS) is a fungal disease responsible for decimating many bat populations in North America. Pseudogymnoascus destructans ( Pd ), the psychrophilic fungus responsible for WNS, prospers in the winter habitat of many hibernating bat species. The immune response that Pd elicits in bats is not yet fully understood; antibodies are produced in response to infection by Pd , but they may not be protective and indeed may be harmful. To understand how bats respond to infection during hibernation, we studied the effect of Pd inoculation on the survival and gene expression of captive hibernating Myotis lucifugus with varying pre-hibernation antifungal antibody titres. We investigated gene expression through the transcription of selected cytokine genes ( Il6 , Il17a , Il1b , Il4 and Ifng ) associated with inflammatory, Th1, Th2 and Th17 immune responses in wing tissue and lymph nodes. We found no difference in survival between bats with low and high anti- Pd titres, although anti- Pd antibody production during hibernation differed significantly between infected and uninfected bats. Transcription of Il6 and Il17a was higher in the lymph nodes of infected bats compared with uninfected bats. Increased transcription of these cytokines in the lymph node suggests that a pro-inflammatory immune response to WNS is not restricted to infected tissues and occurs during hibernation. The resulting Th17 response may be protective in euthermic bats, but because it may disrupt torpor, it could be detrimental during hibernation.

SPE Members Abstract A solution and results of the skin effect of deviated wells presented here suggest that the previous assumption of isotropy would greatly overestimate the absolute magnitude of the skin, in commonly found anisotropies of normal depositional formations and, especially, in laminated reservoirs where the vertical permeability is much smaller. Graphs and correlations of the skin due to slant as a function of the reservoir thickness, index of anisotropy, and well deviation are provided. The performance of a highly deviated well (e.g. 850) can be approximated by a vertical well productivity relationship, augmented by such skin effect, and the results should be practically indistinguishable from those derived from well known horizontal well productivity equations, using equal well production lengths. Introduction Previous work has presented the effects of well deviation from the vertical by means of a negative mechanical skin. An often overlooked implication is that a well test in a highly deviated well should result in such negative skin, otherwise the well is likely to be damaged. Well known solutions of the problem have presumed vertical-to-horizontal permeability isotropy but the recent emergence of horizontal wells has brought an awareness of the importance and frequency of an isotropy. Deviated wells, connecting thin permeable layers that are separated by impermeable streaks, may actually be more attractive than horizontal wells, for reasonable individual layer vertical permeabilities. Studies examining this issue are included in this paper. It is well known that fully completed deviated holes are generally more attractive than vertical wells by virtue of the larger reservoir-to-well exposure. Quantification of this issue has been provided in the literature by Cinco-Ley et al. through the introduction of a mechanical skin effect accounting for this slant. Unfortunately, this work has dealt with permeability-isotropic reservoirs, i.e., for the case where the vertical permeability is equal to the horizontal. This is a highly unrealistic assumption and the industry has become far more aware of the concept of anisotropy after the proliferation of horizontal wells. Once horizontal wells are drilled, the traditional one-permeability perception of a reservoir has to be abandoned. Three permeabilities are thus necessary: two horizontal and one vertical. A very common method of depiction of the vertical-to-horizontal anisotropy is through the index of anisotropy given by the variable Iani: (1) where the horizontal permeability, kH, is itself equal to kxky, the two horizontal components. A typical value of this index of anisotropy for a sandstone reservoir is 3, suggesting a 10:1 ratio between the horizontal and vertical permeabilities. Several publications and reported field cases have shown that this permeability anisotropy is crucial to the success and relative attractiveness of horizontal wells compared to vertical wells in the same reservoir. For thick formations (e.g. h >100 ft) the impact of anisotropy is decisive; for thinner formations (e.g. h < 40 ft) its impact becomes much less pronounced and often forgiving. A comprehensive productivity index model has been presented by Economides et al. In their work they introduced a semi-analytical and highly flexible solution procedure capable of accounting for the effects of anisotropy and thickness on any well configuration. This model is used in this work. P. 279

Summary Induction of cat-86 translation results from the stalling of a ribosome at a discrete location in the leader region of the transcript. Stalling destabilizes an adjacent region of secondary structure that sequesters the cat-86 ribosome binding site, thereby activating cat-86 translation. Two well characterized antibiotics, chloramphenicol and erythromycin, induce cat-86 by stalling a ribosome at the appropriate leader site. Here we demonstrate differences between the two antibiotics with respect to induction. First, induction by chloramphenicol is dependent on nucleotides in the leader sequence that are different from those necessary for erythromycin induction. Second, variants of Bacillus subtilis that are chloramphenicol resistant because of chromosome mutations permit cat-86 induction by chloramphenicol, whereas erythromycin-resistance host mutations block or greatly reduce cat-86 induction by erythromycin. Third, selected strains of B. subtilis bearing alterations in proteins of the 50S ribosomal subunit interfere with cat-86 induction by chloramphenicol, yet these strains are chloramphenicol sensitive. Lastly, induction by chloramphenicol is not reversed by removal of the antibiotic whereas erythromycin induction is reversible. The data indicate that chloramphenicol induction results from an effect of the drug that is not identical to its role as a general inhibitor of ribosome elongation. Induction by erythromycin, on the other hand, could not be distinguished from its antibiotic activity.

The role of crb-86 in cat-86 induction may be a function of both its complementarity with 16S rRNA and the crb -@#@ encoded peptide. Solving the role of crb. as a gene regulatory device will dissect ribosome function in a manner not possible by conventional methodology much in the same vein as other recently discovered variations of ribosome function (12).