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Aim The aim of the present study was to investigate factors associated with denture-induced stomatitis ( DIS) among denture wearers and their oral and hand carriage of Candida. Methods Sociodemographics and denture and oral hygiene practices were investigated using an interviewer-administered questionnaire in denture wearers with DIS ( n = 60) and without DIS ( n = 60) selected using a convenience sampling of patients attending the Department of Prosthetic Dentistry, University of Peradeniya, Sri Lanka. Denture and oral hygiene practices and denture quality were scored. DIS and denture hygiene were classified. Oral and hand carriage of Candida was assessed. Results Middle-aged females were more prone to DIS. The most common DIS pattern was Newton type ΙΙ. The mean age of existing denture of DIS patients was significantly higher than the control. Denture hygiene practice was significantly low in DIS patients. Poor denture hygiene practice and sleep wear of dentures were significantly associated with DIS. The risk of Candida hand carriage in a DIS patient carrying oral Candida was significantly higher than the risk of Candida hand carriage in a healthy denture wearer who carried oral Candida. Conclusion Old dentures, poor denture hygiene, and sleep wear of dentures might contribute to DIS. Oral Candida in denture wearers could lead to Candida hand carriage. [ABSTRACT FROM AUTHOR]

Objectives: We aimed to investigate the effect of brief exposure to sub-cidal concentrations of nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate on the adhesion of oral Candida dubliniensis isolates to the surface of acrylic dentures. Methods: After determining the minimum inhibitory concentration of each drug, 20 oral isolates of C. dubliniensis were exposed to sub-cidal concentrations of the drugs for 1 h. The drugs were then removed by dilution, and the adhesion of the isolates to denture acrylic strips was assessed by an in vitro adhesion assay. Results: Compared to the controls, exposure to nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate suppressed the ability of C. dubliniensis isolates to adhere to acrylic denture surfaces with a reduction of 74.68, 74.27, 57.31, 44.57 and 56.53% (p < 0.001 for all drugs), respectively. Conclusions: Brief exposure to sub-cidal concentrations of anti-mycotics suppressed the adhesion of C. dubliniensis oral isolates to acrylic denture surfaces. © 2014 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Objective: The objective of this study was to determine the cell surface hydrophobicity of 40 oral Candida albicans isolates obtained from smokers, diabetics, asthmatics using steroid inhalers, and healthy individuals, following brief exposure to subtherapeutic concentrations of chlorhexidine gluconate. Materials and Methods: Forty C. albicans oral isolates (10 isolates each from smokers, diabetics, asthmatics using steroid inhalers, and healthy individuals) were exposed to 3 subtherapeutic concentrations of chlorhexidine gluconate (0.00125, 0.0025, and 0.005%) for 30 min. Thereafter, the antiseptic was removed and the cell surface hydrophobicity was measured by a biphasic aqueous-hydrocarbon assay. Results: Compared to the unexposed controls, the cell surface hydrophobicity of C. albicans isolates was suppressed by 5.40% (p > 0.05), 21.17% (p < 0.05), and 44.67% (p < 0.05) following exposure to 0.00125, 0.0025, and 0.005% chlorhexidine gluconate, respectively. Conclusions: A brief period of transient exposure to subtherapeutic concentrations of chlorhexidine gluconate may modulate the cell surface hydrophobicity of C. albicans isolates and thereby may reduce candidal pathogenicity. Copyright © 2012 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Objective: The main objective of this study was to investigate the effect of brief exposure to subtherapeutic concentrations of chlorhexidine gluconate on germ tube formation of Candida albicans isolates obtained from smokers, diabetics, asthmatics using steroid inhalers and healthy individuals. Materials and Methods: Forty isolates of C. albicans were used in this study. All these isolates were quantified for germ tube formation without exposure to the drug and were used as the control group for data analysis. Isolates were also exposed to three subtherapeutic concentrations of chlorhexidine gluconate (0.00125, 0.0025 and 0.005%) for 30 min (limited exposure); the antiseptic was then removed and germ tube formation of these isolates was quantified microscopically following incubation in a germ tube-inducing medium. Results: Compared with the unexposed controls, brief exposure to all concentrations of chlorhexidine gluconate suppressed the ability of the C. albicans isolates to form germ tubes in increasing order by 13.72% (p < 0.001 to p = 0.02), 46.16% (p < 0.001) and 72.46% (p <0.001). Conclusions: These findings show that brief exposure to subtherapeutic concentrations of chlorhexidine gluconate may modulate germ tube formation of C. albicans isolates, thereby suppressing their pathogenicity, and further elucidate the pharmacodynamic mechanisms by which chlorhexidine gluconate may operate in vivo. Copyright © 2011 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]