Different essential oils were tested against the fungus Ascosphaera apis, the causal agent of Chalkbrood Disease of honey bees. Essential oils from lavandin (Lavandula x intermedia Emeric ex Loiseleur), coriander (Coriandrum sativum L.), laurel leaf (Laurus nobilis L.), false camphor (Cinnamomum glandulifera Nees), basil (Ocimum basilicum L.), tagetes (Tagetes minuta L.), rosemary (Rosmarinus officinalis L.) and eucalyptus (Eucalyptus globulus Labill.) were assayed to prove their fungistatic activity at different concentrations: 700, 800 and 900 μ.L/L. Strains of Ascosphaera apis were collected from apiaries of different places of Buenos Aires province (Argentina). At all concentrations tested, coriander oil was the most effective fungistatic control while basil and tagetes oils were effective only at 800 μL/L. No differences in effectiveness of oils were observed against the different strains of Ascosphaera apis.