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The advent of the nanotechnology era offers a unique opportunity for sustainable agriculture, and the contribution of nanoparticles (NPs) to ameliorate abiotic stresses became the new area of interest for researchers due to their special physiochemical characteristics in the biological system. Salinity is a key devastating abiotic factor that hinders the development and yield of rapeseed. On the flip side, the impact of nanoparticles on plant hormones upon salt stress during seed imbibition and germination has been poorly understood. Hence, we aimed to study the influence of nanopriming on plant hormones and germination processes using selenium and zinc oxide nanoparticles (SeNPs and ZnONPs) during seed imbibition and the early seedling stage upon salinity stress. Nanopriming showed a positive effect on final germination percentage, germination rate, seed microstructure, and antioxidant enzyme activity of two rapeseed cultivars under salt stress. Moreover, nano-treatment decreased the expression of abscisic acid related genes BnCYP707A1, 3, and 4 during the priming time and after sowing, where the levels of BnCYP707A1, and 3 genes showed a slightly significant difference between the nanopriming and hydropriming, which gave an evidence that the nanopriming influenced the ABA levels then elevated the seed germination with SeNPs and ZnONPs. Likewise, nanoparticles significantly elevated the expression levels of BnGA20ox, BnGA3ox and BnCPS genes during the germination stage, especially at 24 h after being sown in salt stress. That confirms the positive role of SeNPs and ZnONPs in regulating gibberellic acid level, which increases the germination in primed seeds as compared to unprimed seeds and hydroprimed seeds. Additionally, our results demonstrated that nanopriming regulated the expression level of BnCAM and BnPER during priming time and after sowing, along with the various levels of expression remarkably in BnEXP4 and BnRAB28, especially at 24 h of being sown under salt stress, which promoted seed germination and early seedling growth. Overall, this work provides new insights into mechanisms underlying the interactions of SeNPs and ZnONPs with plant hormones during the seed imbibition and early seedling stage, consequently enhanced plant growth and development. Additionally, these findings portrayed that the application of SeNPs and ZnONPs could be a new strategy and useful approach to enhance tolerance against salinity in rapeseed plants.

Despite heterosis contributing to genetic improvements in crops, root growth heterosis in rapeseed plants is poorly understood at the molecular level. The current study was performed to discover key differentially expressed genes (DEGs) related to heterosis in two hybrids with contrasting root growth performance (FO, high hybrid and FV, low hybrid) based on analysis of the root heterosis effect. Based on comparative transcriptomic analysis, we believe that the overdominance at the gene expression level plays a critical role in hybrid roots’ early biomass heterosis. Our findings imply that a considerable increase in up-regulation of gene expression underpins heterosis. In the FO hybrid, high expression of DEGs overdominant in the starch/sucrose and galactose metabolic pathways revealed a link between hybrid vigor and root growth. DEGs linked to auxin, cytokinin, brassinosteroids, ethylene, and abscisic acid were also specified, showing that these hormones may enhance mechanisms of root growth and the development in the FO hybrid. Moreover, transcription factors such as MYB, ERF, bHLH, NAC, bZIP, and WRKY are thought to control downstream genes involved in root growth. Overall, this is the first study to provide a better understanding related to the regulation of the molecular mechanism of heterosis, which assists in rapeseed growth and yield improvement.

Solenostemma argel (Delile) Hayne is a desert plant that survives harsh environmental conditions with several vital medicinal properties. Salt stress is a major constraint limiting agricultural production around the globe. However, response mechanisms behind the adaptation of S. argel plants to salt stress are still poorly understood. In the current study, we applied an omics approach to explore how this plant adapts to salt stress by integrating transcriptomic and metabolomic changes in the roots and leaves of S. argel plants under salt stress. De novo assembly of transcriptome produced 57,796 unigenes represented by 165,147 transcripts/isoforms. A total of 730 differentially expressed genes (DEGs) were identified in the roots (396 and 334 were up- and down-regulated, respectively). In the leaves, 927 DEGs were identified (601 and 326 were up- and down-regulated, respectively). Gene ontology and Kyoto Encyclopedia of Genes And Genomes pathway enrichment analyses revealed that several defense-related biological processes, such as response to osmotic and oxidative stress, hormonal signal transduction, mitogen-activated protein kinase signaling, and phenylpropanoid biosynthesis pathways are the potential mechanisms involved in the tolerance of S. argel plants to salt stress. Furthermore, liquid chromatography-tandem mass spectrometry was used to detect the metabolic variations of the leaves and roots of S. argel under control and salt stress. 45 and 56 critical metabolites showed changes in their levels in the stressed roots and leaves, respectively; there were 20 metabolites in common between the roots and leaves. Differentially accumulated metabolites included amino acids, polyamines, hydroxycinnamic acids, monolignols, flavonoids, and saccharides that improve antioxidant ability and osmotic adjustment of S. argel plants under salt stress. The results present insights into potential salt response mechanisms in S. argel desert plants and increase the knowledge in order to generate more tolerant crops to salt stress.

Background Elevated ultraviolet-B (UV-B) radiation is potentially deleterious to many organisms specifically crop plants and has become a global challenge. Rice is an exceptionally important staple food which is grown worldwide, and many efforts have been done recently to improve rice varieties against UV-B stress. This current study aims to investigate the effects of exogenous application of β-sitosterol (βSito) on growth improvement and tolerance level of rice plants against prolonged UV-B stress. The physiological and metabolic responses were evaluated in rice plants not supplemented with βSito (Nβ) and those supplemented with βSito (Sβ). Results The Nβ and Sβ plants were grown under non-stress (ns) and under prolonged UV-B stress (uvs) conditions and termed as Nβns, Sβns and Nβuvs, Sβuvs, respectively. The application of βSito contributes positively under non-stress and specifically to UV-B stress in terms of improving numerous physiological parameters associated with growth and development such as shoot and root length, RWC, whole plant biomass, chlorophyll pigments, and photosynthetic-related parameters (Pn, Gs, Tr, WUEi, Fv/Fm, and NPQ) in Sβ compared with Nβ plants. Moreover, enhanced oxidative stress tolerance of Sβuvs vs. Nβuvs plants under stress was attributed to low levels of ROS and substantial trigger in activities of antioxidant enzymes (SOD, POD, CAT, and APX). Metabolic analysis was performed using GC-TOFMS, which revealed higher accumulation of several key metabolites including organic acids, sugars, amino acids, and others in Sβuvs vs. Nβuvs plants, which were mainly reduced in Nβ plants under stress vs. non-stress conditions. Conclusion These results provide useful data regarding the important role of βSito on growth maintenance and modulation of several metabolites associated with osmotic and redox adjustments during UV-B stress tolerance in rice plants. Importantly, βSito-regulated plasticity could further be explored specifically in relation to different environmental stresses in other economically useful crop plants.

Date palm (Phoenix dactylifera) is one of the most widespread fruit crop species and can tolerate drastic environmental conditions that may not be suitable for other fruit species. Excess UV-B stress is one of the greatest concerns for date palm trees and can cause genotoxic effects. Date palm responds to UV-B irradiation through increased DEG expression levels and elaborates upon regulatory metabolic mechanisms that assist the plants in adjusting to this exertion. Sixty-day-old Khalas date palm seedlings (first true-leaf stage) were treated with UV-B (wavelength, 253.7 nm, intensity, 75 μW cm−2 for 72 h (16 h of UV light and 8 h of darkness). Transcriptome analysis revealed 10,249 and 12,426 genes whose expressions were upregulated and downregulated, respectively, compared to the genes in the control. Furthermore, the differentially expressed genes included transcription factor-encoding genes and chloroplast- and photosystem-related genes. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to detect metabolite variations. Fifty metabolites, including amino acids and flavonoids, showed changes in levels after UV-B excess. Amino acid metabolism was changed by UV-B irradiation, and some amino acids interacted with precursors of different pathways that were used to synthesize secondary metabolites, i.e., flavonoids and phenylpropanoids. The metabolite content response to UV-B irradiation according to hierarchical clustering analysis showed changes in amino acids and flavonoids compared with those of the control. Amino acids might increase the function of scavengers of reactive oxygen species by synthesizing flavonoids that increase in response to UV-B treatment. This study enriches the annotated date palm unigene sequences and enhances the understanding of the mechanisms underlying UV-B stress through genetic manipulation. Moreover, this study provides a sequence resource for genetic, genomic and metabolic studies of date palm.

Background Phytochromes are plant photoreceptors that have long been associated with photomorphogenesis in plants; however, more recently, their crucial role in the regulation of variety of abiotic stresses has been explored. Chilling stress is one of the abiotic factors that severely affect growth, development, and productivity of crops. In the present work, we have analyzed and compared physiological, biochemical, and molecular responses in two contrasting phytochrome mutants of tomato, namely aurea (aur) and high pigment1 (hp1), along with wild-type cultivar Micro-Tom (MT) under chilling stress. In tomato, aur is phytochrome-deficient mutant while hp1 is a phytochrome-sensitive mutant. The genotype-specific physiological, biochemical, and molecular responses under chilling stress in tomato mutants strongly validated phytochrome-mediated regulation of abiotic stress. Results Here, we demonstrate that phytochrome-sensitive mutant hp1 show improved performance compared to phytochrome-deficient mutant aur and wild-type MT plants under chilling stress. Interestingly, we noticed significant increase in several photosynthetic-related parameters in hp1 under chilling stress that include photosynthetic rate, stomatal conductance, stomatal aperture, transpiration rate, chlorophyll a and carotenoids. Whereas most parameters were negatively affected in aur and MT except a slight increase in carotenoids in MT plants under chilling stress. Further, we found that PSII quantum efficiency (Fv/Fm), PSII operating efficiency (Fq′/Fm′), and non-photochemical quenching (NPQ) were all positively regulated in hp1, which demonstrate enhanced photosynthetic performance of hp1 under stress. On the other hand, Fv/Fm and Fq′/Fm′ were decreased significantly in aur and wild-type plants. In addition, NPQ was not affected in MT but declined in aur mutant after chilling stress. Noticeably, the transcript analysis show that PHY genes which were previously reported to act as molecular switches in response to several abiotic stresses were mainly induced in hp1 and repressed in aur and MT in response to stress. As expected, we also found reduced levels of malondialdehyde (MDA), enhanced activities of antioxidant enzymes, and higher accumulation of protecting osmolytes (soluble sugars, proline, glycine betaine) which further elaborate the underlying tolerance mechanism of hp1 genotype under chilling stress. Conclusion Our findings clearly demonstrate that phytochrome-sensitive and phytochrome-deficient tomato mutants respond differently under chilling stress thereby regulating physiological, biochemical, and molecular responses and thus establish a strong link between phytochromes and their role in stress tolerance.

Salinity stress negatively affects the plant's developmental stages through micronutrient imbalance. As an essential micronutrient, ZnO can substitute Na+ absorption under saline conditions. Therefore, nanoparticles as technological innovation, improve the plant growth efficiency under biotic and abiotic stresses. Nano-priming has become widely applicable in agricultural research during the last decade. The current study was conducted to highlight the impact of ZnONPs priming on seedling biological processes under 150 mM of NaCl using two rapeseed cultivars during the early seedling stage. All concentrations of ZnONPs increased the germination parameters i.e., FG%, GR, VI (I), and VI (II). Meanwhile, the high concentration (ZnO 100%) showed the highest increase in shoot length (9.60% and 25.63%), root length (41.64% and 48.17%) for Yang You 9 and Zhong Shuang 11 over hydro-priming, respectively, as well as biomass. Additionally, nano-priming improved the proline, soluble sugar, and soluble protein contents as a result of osmotic protection modulation. Moreover, nano-priming alleviated ROS and biosynthesis pigments through the reduction of accumulated (H2O2) and (O2−), and chlorophyll degradation, respectively, also enhanced antioxidant adjustment via improving the plant defense system. Nano-priming substituted the Na+ by Zn2+, K+, and Ca2+, and compensated the deficit of micronutrients, thus reduced the Na+ toxicity in the cell cytosol. To track the effects of priming during seed imbibition, it noticed that ZnO 100% and ZnO 100%+S increased the Linoleic and Linolenic acids among the studied fatty acids composition by 12.02%, 7.59%, 13.27%, and 10.38% (Yang You 9), 7.42%, 2.77%, 2.93%, and 1.49% (Zhong Shuang 11) over the hydro-priming, respectively. Moreover, the gene expression patterns of BnCAM and BnPER reflected the enhancement of germination levels, notably under the influence of ZnO 100% priming, which increased the level of BnCAM by 70.42% and 111.9% in Yang You 9 and Zhong Shuang 11, respectively. Consequently, ZnO nano-priming enhanced the seedling development through the biosynthesis of pigments, osmotic protection, reduction of ROS accumulation, adjustment of antioxidant enzymes, and improvement of the nutrient absorption, thus enhancing the economic yield under saline conditions.

Additional file 1: Supplementary Table S1. Primers list used for qRT-PCR based tomato PHY genes expression.

A constant supply of soil nutrients is critical for the normal growth and development of plants. However, most environments are unstable and this variability depends on numerous factors that includ...

Improving nutritional fruit quality and impacts important agro-traits such as biotic or abiotic stresses are extremely important for human civilization. Our previous study reported that manipulation of SlMX1 gene enhanced carotenoids accumulation and drought resistance in tomato. Here, RNA-Seq analysis proved to be a very useful tool to provide insights into the regulatory mechanisms of SlMX1 involved in stress resistance and enhanced secondary metabolites. Physiological analysis showed that over-expression of SlMX1 results in substantially increased broad-spectrum tolerance to a wide-range of abiotic and biotic (fungus, bacteria, virus and insects) stresses in tomato. This research appears to be of remarkable interest because enhanced terpenoids content has been achieved by increasing trichome density. In addition, we reported two types of trichome which seems to be aberrant types in tomato. This study unravels the mechanism of regulation of SlMX1, which simultaneously modulates resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways.

Plants are the ultimate source of nutrients in the human diet. To ensure adequate availability of high quality food for an increasing world population, traits including improved tolerance of stresses and nutrient levels need to be selected in crops, both individually and in combination. Here we report the identification of SlMX1 encoding a MIXTA-like MYB transcription factor in tomato that simultaneously modulates drought resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways. Over-expression of SlMX1 results in substantially increased drought tolerance and improved fruit quality, while knocking down SlMX1 resulted in the opposite phenotypes. Our study indicates an effective way with multiple beneficial traits by genetic engineering of a single regulatory gene and can be a novel approach to breeding crops.

Potato (Solanum tuberosum) late blight caused by oomycete Phytophthora infestans (Pi) is highly destructive to potato yield and cost huge losses each year. Regulation of a network of transcription machinery, controlled by transcription factors (TF’s), is required to overcome the susceptibility. WRKY TF’s are known to regulate transcription machinery upon biotic and abiotic stresses in different crop plants. We cloned and characterized a WRKY gene, StWRKY1, from potato cDNA synthesized from Pi infested leaves. StWRKY1 protein localized typically in the nucleus. Overexpression (OE) of StWRKY1 positively regulates Pi resistance as well as drought tolerance in transgenic potato. The elevated resistance in OE lines was co-related with higher accumulation of pathogenesis-related (PR) genes as compared to untransformed control plants. Interestingly, increased susceptibility of co-suppression (CSP) plants was associated with down regulation of PR genes expression. Moreover, transgenic lines overexpressing StWRKY1 showed tolerance in terms of less rate of water loss, during dehydration assay. Importantly, expression of StWRKY1 was upregulated upon treatment with plant hormones, suggesting its involvement in basal signal transduction pathway. Overall, our findings provided evidence that StWRKY1 positively regulate biotic and abiotic stress resistance thereby modulating plant basal defense networks, thus play a significant role for crop improvement.

Citrus is a perennial woody crop of global economic importance. However, the regulatory mechanism of fruit ripening in citrus remains poorly understood. To explore the regulatory roles of transcription factors in fruit ripening, CgDREB ( d ehydration r esponsive e lement b inding) gene was characterized and was functionally overexpressed in tomato ( Solanum lycopersicum, cv. Ailsa Craig), resulting in smaller fruit size. Therefore, two independent lines with the highest expression of CgDREB were selected to study the metabolite profile in the flesh and peel of tomato fruits during ripening using gas chromatography–mass spectrometry. The results revealed that the levels of organic acids and sugars generally increased in both the flesh and peel of tomato. In addition, a significant increase of ethylene production was observed at the breaker stage, indicating a strong correlation between fruit ripening and ethylene release. Taken together, our results provide insights into the important role of CgDREB gene in regulating fruit ripening properties.

Adaptation to environmental changes is an important fitness trait for crop development. Photoperiod is an essential factor in seasonal control of flowering time. Sensing of day-length requires an interaction between the Photoperiod and the endogenous rhythms that is controlled by plant circadian clock. Thus, circadian clock is a critical regulator and internal molecular time-keeping mechanism, controlling key agricultural traits in crop plants such as the ability to adjust their growth and physiology to anticipate diurnal environmental changes. Here, we describe the gene Tomato Dof Daily Fluctuations 1 (TDDF1), which is involved in circadian regulation and stress resistance. Large daily oscillations in TDDF1 expression were retained after transferring to continuous dark (DD) or light (LL) conditions. Interestingly, overexpressing TDDF1 induce early flowering in tomato through up-regulation of the flowering-time control genes, moreover, by protein-protein interaction with the floral inducer SFT gene. Notably, overexpressing TDDF1 in tomato was associated with chlorophyll overaccumulation by up-regulating the related biosynthetic genes. TDDF1 expression results in improved drought, salt, various hormones stress tolerance alongwith resistance to late blight caused by Phytophthora infestans. This study can be a distinctive strategy to improve other economically important crops.

Citric acid plays an important role in fresh fruit flavor and its adaptability to post-harvest storage conditions. In order to explore organic acid regulatory mechanisms in post-harvest citrus fruit, systematic biological analyses were conducted on stored Hirado Buntan Pummelo (HBP; Citrus grandis) fruits. High-performance capillary electrophoresis, subcellular organelle expression microarray, real-time quantitative reverse transcription polymerase chain reaction, gas chromatography mass spectrometry (GC-MS), and conventional physiological and biochemical analyses were undertaken. The results showed that the concentration of organic acids in HBP underwent a regular fluctuation. GC-MS-based metabolic profiling indicated that succinic acid, γ-aminobutyric acid (GABA), and glutamine contents increased, but 2-oxoglutaric acid content declined, which further confirmed that the GABA shunt may have some regulatory roles in organic acid catabolism processes. In addition, the concentration of organic acids was significantly correlated with senescence-related physiological processes, such as hydrogen peroxide content as well as superoxide dismutase and peroxidase activities, which showed that organic acids could be regarded as important parameters for measuring citrus fruit post-harvest senescence processes.

Citric acid plays an important role in fresh fruit flavor and its adaptability to post-harvest storage conditions. In order to explore organic acid regulatory mechanisms in post-harvest citrus fruit, systematic biological analyses were conducted on stored Hirado Buntan Pummelo (HBP; Citrus grandis) fruits. High-performance capillary electrophoresis, subcellular organelle expression microarray, real-time quantitative reverse transcription polymerase chain reaction, gas chromatography mass spectrometry (GC-MS), and conventional physiological and biochemical analyses were undertaken. The results showed that the concentration of organic acids in HBP underwent a regular fluctuation. GC-MS-based metabolic profiling indicated that succinic acid, γ-aminobutyric acid (GABA), and glutamine contents increased, but 2-oxoglutaric acid content declined, which further confirmed that the GABA shunt may have some regulatory roles in organic acid catabolism processes. In addition, the concentration of organic acids was significantly correlated with senescence-related physiological processes, such as hydrogen peroxide content as well as superoxide dismutase and peroxidase activities, which showed that organic acids could be regarded as important parameters for measuring citrus fruit post-harvest senescence processes.