Search

Your search keyword '"Elshenawy, Mohamed"' showing total 112 results
Start Over Author "Elshenawy, Mohamed"
112 results on '"Elshenawy, Mohamed"'

2. Characterizing the impact of using features extracted from pre-trained models on the quality of video captioning sequence-to-sequence models

Author

Hammad, Menatallh, Hammad, May, and Elshenawy, Mohamed

Subjects
Computer Science - Computer Vision and Pattern Recognition
Abstract

The task of video captioning, that is, the automatic generation of sentences describing a sequence of actions in a video, has attracted an increasing attention recently. The complex and high-dimensional representation of video data makes it difficult for a typical encoder-decoder architectures to recognize relevant features and encode them in a proper format. Video data contains different modalities that can be recognized using a mix image, scene, action and audio features. In this paper, we characterize the different features affecting video descriptions and explore the interactions among these features and how they affect the final quality of a video representation. Building on existing encoder-decoder models that utilize limited range of video information, our comparisons show how the inclusion of multi-modal video features can make a significant effect on improving the quality of generated statements. The work is of special interest to scientists and practitioners who are using sequence-to-sequence models to generate video captions., Comment: Submitted to conference ICPRAI2020

Published
2019
Full Text
View/download PDF

3. Lis1 activates dynein motility by modulating its pairing with dynactin.

Author

Elshenawy, Mohamed, Kusakci, Emre, Volz, Sara, Baumbach, Janina, Bullock, Simon, and Yildiz, Ahmet

Subjects
Animals, Cell Line, Dynactin Complex, Dyneins, Humans, Kinesins, Microtubule-Associated Proteins, Microtubules, Protein Binding, Protein Transport, Sf9 Cells, Swine
Abstract

Lissencephaly-1 (Lis1) is a key cofactor for dynein-mediated intracellular transport towards the minus-ends of microtubules. It remains unclear whether Lis1 serves as an inhibitor or an activator of mammalian dynein motility. Here we use single-molecule imaging and optical trapping to show that Lis1 does not directly alter the stepping and force production of individual dynein motors assembled with dynactin and a cargo adaptor. Instead, Lis1 promotes the formation of an active complex with dynactin. Lis1 also favours the recruitment of two dyneins to dynactin, resulting in increased velocity, higher force production and more effective competition against kinesin in a tug-of-war. Lis1 dissociates from motile complexes, indicating that its primary role is to orchestrate the assembly of the transport machinery. We propose that Lis1 binding releases dynein from its autoinhibited state, which provides a mechanistic explanation for why Lis1 is required for efficient transport of many dynein-associated cargos in cells.

Published
2020

4. Cargo adaptors regulate stepping and force generation of mammalian dynein–dynactin

Author

Elshenawy, Mohamed M, Canty, John T, Oster, Liya, Ferro, Luke S, Zhou, Zhou, Blanchard, Scott C, and Yildiz, Ahmet

Subjects
Biochemistry and Cell Biology, Biological Sciences, 1.1 Normal biological development and functioning, Underpinning research, Generic health relevance, Animals, Dynactin Complex, Dyneins, Humans, Protein Binding, Medicinal and Biomolecular Chemistry, Biochemistry & Molecular Biology, Biochemistry and cell biology, Medicinal and biomolecular chemistry
Abstract

Cytoplasmic dynein is an ATP-driven motor that transports intracellular cargos along microtubules. Dynein adopts an inactive conformation when not attached to a cargo, and motility is activated when dynein assembles with dynactin and a cargo adaptor. It was unclear how active dynein-dynactin complexes step along microtubules and transport cargos under tension. Using single-molecule imaging, we showed that dynein-dynactin advances by taking 8 to 32-nm steps toward the microtubule minus end with frequent sideways and backward steps. Multiple dyneins collectively bear a large amount of tension because the backward stepping rate of dynein is insensitive to load. Recruitment of two dyneins to dynactin increases the force generation and the likelihood of winning against kinesin in a tug-of-war but does not directly affect velocity. Instead, velocity is determined by cargo adaptors and tail-tail interactions between two closely packed dyneins. Our results show that cargo adaptors modulate dynein motility and force generation for a wide range of cellular functions.

Published
2019

5. Casemix, management, and mortality of patients receiving emergency neurosurgery for traumatic brain injury in the Global Neurotrauma Outcomes Study: a prospective observational cohort study

Author

Abbas, Ghayur, Abdallah, Omar Ibrahim, Abdel-Lateef, Ahmed, Abdifatah, Khalif, Abdullateef, Awfa, Abeygunaratne, Ruvini, Aboellil, Mostafa, Adam, Abass, Adams, Robert, Adeleye, Amos, Adeolu, Augustine, Adji, Novan Krisno, Afianti, Nur, Agarwal, Sudarsan, Aghadi, Ifeanyi Kene, Aguilar, Paúl Martín Méndez, Ahmad, Syeda Rida, Ahmed, Daniyal, Ahmed, Nafees, Aizaz, Haider, Aji, Yunus Kuntawi, Alamri, Alex, Alberto, Augusto Jacinto Mussindo, Alcocer, Luis Alcocer, Alfaro, Lesly Gonzales, Al-Habib, Amro, Alhourani, Ahmad, Ali, Syed Muhammad Rafay, Alkherayf, Fahad, AlMenabbawy, Ahmed, Alshareef, Aliyah, Aminullah, Muhammad Adil s/o, Amjad, Madeha, Amorim, Robson Luis Oliveira de, Anbazhagan, Sathiaprabhu, Andrade, Almir, Antar, Waleed, Anyomih, Theophilus T.K., Aoun, Salah, Apriawan, Tedy, Armocida, Daniele, Arnold, Paul, Arraez, Miguel, Assefa, Temesgen, Asser, Andres, Athiththan, S.P., Attanayake, Deepal, Aung, Maung Maung, Avi, Allan, Ayala, Victor Enrique Antolinez, Azab, Mohammed, Azam, Gaousul, Azharuddin, Mohd, Badejo, Olukemi, Badran, Mohamed, Baig, Azam Ali, Baig, Rehman Ali, Bajaj, Ankur, Baker, Paul, Bala, Renu, Balasa, Artur, Balchin, Ross, Balogun, James, Ban, Vin Shen, Bandi, Bharath Kumar Reddy, Bandyopadhyay, Soham, Bank, Matthew, Barthelemy, Ernest, Bashir, Mohammed Talha, Basso, Luciano Silveira, Basu, Surajit, Batista, Auricelio, Bauer, Marlies, Bavishi, Devi, Beane, Abi, Bejell, Shmuel, Belachew, Anteneh, Belli, Antonio, Belouaer, Amani, Bendahane, Najia El Abbadi, Benjamin, Okanga, Benslimane, Youssef, Benyaiche, Chaymae, Bernucci, Claudio, Berra, Luigi Valentino, Bhebe, Arnold, Bimpis, Alexios, Blanaru, Diana, Bonfim, Jean Claude, Borba, Luis A B, Borcek, Alp Ozgun, Borotto, Erika, Bouhuwaish, Ahmad Elmabri Mohammad, Bourilhon, Facundo, Brachini, Gioia, Breedon, Joshua, Broger, Maximilian, Brunetto, Giacoma Maria Floriana, Bruzzaniti, Placido, Budohoska, Natalia, Burhan, Hira, Calatroni, Maximiliano Luis, Camargo, Catherine, Cappai, Pier Francesco, Cardali, Salvatore Massimiliano, Castaño-Leon, Ana M, Cederberg, David, Celaya, Mikel, Cenzato, Marco, Challa, Lakshmi Madhavi, Charest, Dhanny, Chaurasia, Bipin, Chenna, Rabah, Cherian, Iype, Ching'o, Juliana Henry, Chotai, Tejas, Choudhary, Ajay, Choudhary, Nabeel, Choumin, Florence, Cigic, Tomislav, Ciro, Juan, Conti, Carlo, Corrêa, Antônio Carlos de Souza, Cossu, Giulia, Couto, Maíra Piani, Cruz, Aurora, D'Silva, Divya, D'Aliberti, Giuseppe Antonio, Dampha, Lamin, Daniel, Roy Thomas, Dapaah, Andrew, Darbar, Aneela, Dascalu, Gabriel, Dauda, Happy Amos, Davies, Owain, Delgado-Babiano, Andrea, Dengl, Markus, Despotovic, Marko, Devi, Indira, Dias, Celeste, Dirar, Mohamed, Dissanayake, Melina, Djimbaye, Hananiah, Dockrell, Simon, Dolachee, Ali, Dolgopolova, Julija, Dolgun, Muge, Dow, Abdalrouf, Drusiani, Davide, Dugan, Artjom, Duong, Dinh Tuan, Duong, Trung Kien, Dziedzic, Tomasz, Ebrahim, Ali, El Fatemi, Nizar, El Helou, Antonios El, El Maaqili, Rachid El, El Mostarchid, Brahim El, El Ouahabi, Abdessamad El, Elbaroody, Mohammad, El-Fiki, Ahmed, El-Garci, Ahmed, El-Ghandour, Nasser M.F., Elhadi, Muhammed, Elleder, Vanessa, Elrais, Safa, El-shazly, Mohamed, Elshenawy, Mohamed, Elshitany, Hesham, El-Sobky, Omar, Emhamed, Marwa, Enicker, Basil, Erdogan, Onur, Ertl, Sebastian, Esene, Ignatius, Espinosa, Omar Ocampo, Fadalla, Tarig, Fadelalla, Mohammed, Faleiro, Rodrigo Moreira, Fatima, Nida, Fawaz, Charbel, Fentaw, Assefa, Fernandez, Carla Eiriz, Ferreira, Ana, Ferri, Francesco, Figaji, Tony, Filho, Emerson L B, Fin, Loic, Fisher, Benjamin, Fitra, Fitra, Flores, Alexis Palpan, Florian, Ioan Stefan, Fontana, Vincenzo, Ford, Lauren, Fountain, Daniel, Frade, Jose Maria Roda, Fratto, Antonio, Freyschlag, Christian, Gabin, Aranzazu Sánchez, Gallagher, Clare, Ganau, Mario, Gandia-Gonzalez, Maria Luisa, Garcia, Andoni, Garcia, Borja Hernandez, Garusinghe, Sanjeewa, Gebreegziabher, Biniam, Gelb, Adrian, George, Jerome St, Germanò, Antonino Francesco, Ghetti, Ilaria, Ghimire, Prajwal, Giammarusti, Alessandro, Gil, Jose Luis, Gkolia, Panagiota, Godebo, Yoseph, Gollapudi, Prakash Rao, Golubovic, Jagos, Gomes, Jeremias Fernando, Gonzales, Javier, Gormley, William, Gots, Alexander, Gribaudi, Giulia Letizia, Griswold, Dylan, Gritti, Paolo, Grobler, Ruan, Gunawan, Rudy, Hailemichael, Birhanu, Hakkou, Elmehdi, Haley, Mark, Hamdan, Alhafidz, Hammed, Ali, Hamouda, Waeel, Hamzah, Nurul Ashikin, Han, Nyein Latt, Hanalioglu, Sahin, Haniffa, Rashan, Hanko, Martin, Hanrahan, John, Hardcastle, Timothy, Hassani, Fahd Derkaoui, Heidecke, Volkmar, Helseth, Eirik, Hernández-Hernández, Miguel Ángel, Hickman, Zachary, Hoang, Le Minh Chau, Hollinger, Alexa, Horakova, Lenka, Hossain-Ibrahim, Kismet, Hou, Boru, Hoz, Samer, Hsu, Janine, Hunn, Martin, Hussain, Madiha, Iacopino, Giorgia, Ideta, Mylena Miki Lopes, Iglesias, Irene, Ilunga, Ali, Imtiaz, Nafiz, Islam, Rafiza, Ivashchenko, Serge, Izirouel, Karim, Jabal, Mohamed Sobhi, Jabal, Soubhi, Jabang, John Nute, Jamjoom, Aimun, Jan, Irfan, Jarju, Landing BM, Javed, Saad, Jelaca, Bojan, Jhawar, Sukhdeep Singh, Jiang, Ting Ting, Jimenez, Fernando, Jiris, Jorge, Jithoo, Ron, Johnson, Walt, Joseph, Mathew, Joshi, Rameshman, Junttila, Eija, Jusabani, Mubashir, Kache, Stephen Akau, Kadali, Satyavara Prasad, Kalkmann, Gabriela F, Kamboh, Usman, Kandel, Hitham, Karakus, Ahmet Kamil, Kassa, Mengistu, Katila, Ari, Kato, Yoko, Keba, Martin, Kehoe, Kristy, Kertmen, Huseyin Hayri, Khafaji, Soha, Khajanchi, Monty, Khan, Mohammed, Khan, Muhammad Mukhtar, Khan, Sohail Daud, Khizar, Ahtesham, Khriesh, Amir, Kierońska, Sara, Kisanga, Paul, Kivevele, Boniface, Koczyk, Kacper, Koerling, Anna-Lucia, Koffenberger, Danielle, Kõiv, Kennet, Kõiv, Leho, Kolarovszki, Branislav, König, Marton, Könü-Leblebicioglu, Dilek, Koppala, Santhoshi Devi, Korhonen, Tommi, Kostkiewicz, Boguslaw, Kostyra, Kacper, Kotakadira, Srinivas, Kotha, Arjun Reddy, Kottakki, Madhu Narayana Rao, Krajcinovic, Nenad, Krakowiak, Michal, Kramer, Andreas, Krishnamoorthy, Selvamuthukumaran, Kumar, Ashok, Kumar, Pankaj, Kumar, Pradhumna, Kumarasinghe, Nilaksha, Kuncha, Gowtham, Kutty, Raja K., Laeke, Tsegazeab, Lafta, Ghazwan, Lammy, Simon, Lapolla, Pierfrancesco, Lardani, Jacopo, Lasica, Nebojsa, Lastrucci, Giancarlo, Launey, Yoann, Lavalle, Laura, Lawrence, Tim, Lazaro, Albert, Lebed, Vitalii, Leinonen, Ville, Lemeri, Lawrence, Levi, Leon, Lim, Jia Yi, Lim, Xiao Yi, Linares-Torres, Jorge, Lippa, Laura, Lisboa, Lurdes, Liu, Jinfang, Liu, Ziyuan, Lo, William B, Lodin, Jan, Loi, Federico, Londono, Daniella, Lopez, Pedro Antonio Gomez, López, Cristina Barceló, Lotbiniere-Bassett, Madeleine De, Lulens, Rihards, Luna, Facundo Hector, Luoto, Teemu, M.V., Vijaya Sekhar, Mabovula, Ndyebo, MacAllister, Matthew, Macie, Alcina Americo, Maduri, Rodolfo, Mahfoud, Moufid, Mahmood, Ashraf, Mahmoud, Fathia, Mahoney, Dominic, Makhlouf, Wissam, Malcolm, George, Malomo, Adefolarin, Malomo, Toluyemi, Mani, Manoranjitha Kumari, Marçal, Tomás Gazzinelli, Marchello, Jacopo, Marchesini, Nicolò, Marhold, Franz, Marklund, Niklas, Martín-Láez, Rubén, Mathaneswaran, Vickneswaran, Mato-Mañas, David José, Maye, Helen, McLean, Aaron Lawson, McMahon, Catherine, Mediratta, Saniya, Mehboob, Mehreen, Meneses, Alisson, Mentri, Nesrine, Mersha, Hagos, Mesa, Ana Milena, Meyer, Cristy, Millward, Christopher, Mimbir, Salomao Amone, Mingoli, Andrea, Mishra, Parashruram, Mishra, Tejesh, Misra, Basant, Mittal, Siddharth, Mohammed, Imran, Moldovan, Ioana, Molefe, Masechaba, Moles, Alexis, Moodley, Preston, Morales, Mario Augusto Narváez, Morgan, Lucy, Morillo, German Del Castillo, Moustafa, Wahab, Moustakis, Nikolaos, Mrichi, Salma, Munjal, Satya Shiva, Muntaka, Abdul-Jalilu Mohammed, Naicker, Denver, Nakashima, Paulo E H, Nandigama, Pratap Kumar, Nash, Samantha, Negoi, Ionut, Negoita, Valetina, Neupane, Samundra, Nguyen, Manh Hung, Niantiarno, Fajar Herbowo, Noble, Abbi, Nor, Mohd Arman Muhamad, Nowak, Blazej, Oancea, Andrei, O'Brien, Frazer, Okere, Oghenekevwe, Olaya, Sandra, Oliveira, Leandro, Oliveira, Louise Makarem, Omar, Fatma, Ononeme, Okezi, Opšenák, René, Orlandini, Simone, Osama, Alrobah, Osei-Poku, Dorcas, Osman, Haytham, Otero, Alvaro, Ottenhausen, Malte, Otzri, Shuli, Outani, Oumaima, Owusu, Emmanuel Abem, Owusu-Agyemang, Kevin, Ozair, Ahmad, Ozoner, Baris, Paal, Elli, Paiva, Mauro Sérgio, Paiva, Wellingson, Pandey, Sharad, Pansini, Gastone, Pansini, Luigi, Pantel, Tobias, Pantelas, Nikolaos, Papadopoulos, Konstantinos, Papic, Vladimir, Park, Kee, Park, Nick, Paschoal, Eric Homero Albuquerque, Paschoalino, Mylla Christie de Oliveira, Pathi, Rajesh, Peethambaran, Anilkumar, Pereira, Thiago Andrade, Perez, Irene Panero, Pérez, Claudio José Piqueras, Periyasamy, Tamilanandh, Peron, Stefano, Phillips, Michael, Picazo, Sofía Sotos, Pinar, Ertugrul, Pinggera, Daniel, Piper, Rory, Pirakash, Pathmanesan, Popadic, Branko, Posti, Jussi P., Prabhakar, Rajmohan Bhanu, Pradeepan, Sivanesalingam, Prasad, Manjunath, Prieto, Paola Calvachi, Prince, Ron, Prontera, Andrea, Provaznikova, Eva, Quadros, Danilo, Quintero, Nezly Jadid Romero, Qureshi, Mahmood, Rabiel, Happiness, Rada, Gabriel, Ragavan, Sivagnanam, Rahman, Jueria, Ramadhan, Omar, Ramaswamy, Padma, Rashid, Sakina, Rathugamage, Jagath, Rätsep, Tõnu, Rauhala, Minna, Raza, Asif, Reddycherla, Naga Raju, Reen, Linus, Refaat, Mohamed, Regli, Luca, Ren, Haijun, Ria, Antonio, Ribeiro, Thales Francisco, Ricci, Alessandro, Richterová, Romana, Ringel, Florian, Robertson, Faith, Rocha, Catarina Mayrink Siqueira Cabral, Rogério, Juvenal de Souza, Romano, Adan Anibal, Rothemeyer, Sally, Rousseau, Gail Rousseau Gail, Roza, Ranette, Rueda, Kevin David Farelo, Ruiz, Raiza, Rundgren, Malin, Rzeplinski, Radoslaw, S.Chandran, Raj, Sadayandi, Ramesh Andi, Sage, William, Sagerer, André Norbert Josef, Sakar, Mustafa, Salami, Mohcine, Sale, Danjuma, Saleh, Youssuf, Sánchez-Viguera, Cristina, Sandila, Saning'o, Sanli, Ahmet Metin, Santi, Laura, Santoro, Antonio, Santos, Aieska Kellen Dantas Dos, Santos, Samir Cezimbra dos, Sanz, Borja, Sapkota, Shabal, Sasidharan, Gopalakrishnan, Sasillo, Ibrahim, Satoskar, Rajeev, Sayar, Ali Caner, Sayee, Vignesh, Scheichel, Florian, Schiavo, Felipe Lourenzon, Schupper, Alexander, Schwarz, Andreas, Scott, Teresa, Seeberger, Esther, Segundo, Claudionor Nogueira Costa, Seidu, Anwar Sadat, Selfa, Antonio, Selmi, Nazan Has, Selvarajah, Claudiya, Şengel, Necmiye, Seule, Martin, Severo, Luiz, Shah, Purva, Shahzad, Muhammad, Shangase, Thobekile, Sharma, Mayur, Shiban, Ehab, Shimber, Emnet, Shokunbi, Temitayo, Siddiqui, Kaynat, Sieg, Emily, Siegemund, Martin, Sikder, Shahidur Rahman, Silva, Ana Cristina Veiga, Silva, Ana, Silva, Pedro Alberto, Singh, Deepinder, Skadden, Carly, Skola, Josef, Skouteli, Eirini, Słoniewski, Pawel, Smith, Brandon, Solanki, Guirish, Solla, Davi Fontoura, Solla, Davi, Sonmez, Ozcan, Sönmez, Müge, Soon, Wai Cheong, Stefini, Roberto, Stienen, Martin Nikolaus, Stoica, Bogdan, Stovell, Matthew, Suarez, Maria Natalia, Sulaiman, Alaa, Suliman, Mazin, Sulistyanto, Adi, Sulubulut, Şeniz, Sungailaite, Sandra, Surbeck, Madlen, Szmuda, Tomasz, Taddei, Graziano, Tadele, Abraham, Taher, Ahmed Saleh Ahmed, Takala, Riikka, Talari, Krishna Murthy, Tan, Bih Huei, Tariciotti, Leonardo, Tarmohamed, Murad, Taroua, Oumayma, Tatti, Emiliano, Tenovuo, Olli, Tetri, Sami, Thakkar, Poojan, Thango, Nqobile, Thatikonda, Satish Kumar, Thesleff, Tuomo, Thomé, Claudius, Thornton, Owen, Timmons, Shelly, Timoteo, Eva Ercilio, Tingate, Campbell, Tliba, Souhil, Tolias, Christos, Toman, Emma, Torres, Ivan, Torres, Luis, Touissi, Youness, Touray, Musa, Tropeano, Maria Pia, Tsermoulas, Georgios, Tsitsipanis, Christos, Turkoglu, Mehmet Erhan, Uçkun, Özhan Merzuk, Ullman, Jamie, Ungureanu, Gheorghe, Urasa, Sarah, Ur-Rehman, Obaid, Uysal, Muhammed, Vakis, Antonios, Valeinis, Egils, Valluru, Vaishali, Vannoy, Debby, Vargas, Pablo, Varotsis, Phillipos, Varshney, Rahul, Vats, Atul, Veljanoski, Damjan, Venturini, Sara, Verma, Abhijit, Villa, Clara, Villa, Genaro, Villar, Sofia, Villard, Erin, Viruez, Antonio, Voglis, Stefanos, Vulekovic, Petar, Wadanamby, Saman, Wagner, Katherine, Walshe, Rebecca, Walter, Jan, Waseem, Marriam, Whitworth, Tony, Wijeyekoon, Ruwani, Williams, Adam, Wilson, Mark, Win, Sein, Winarso, Achmad Wahib Wahju, Ximenes, Abraão Wagner Pessoa, Yadav, Anurag, Yadav, Dipak, Yakoub, Kamal Makram, Yalcinkaya, Ali, Yan, Guizhong, Yaqoob, Eesha, Yepes, Carlos, Yılmaz, Ayfer Nazmiye, Yishak, Betelehem, Yousuf, Farhat Basheer, Zahari, Muhammad Zamzuri, Zakaria, Hussein, Zambonin, Diego, Zavatto, Luca, Zebian, Bassel, Zeitlberger, Anna Maria, Zhang, Furong, Zheng, Fengwei, Ziga, Michal, Clark, David, Joannides, Alexis, Adeleye, Amos Olufemi, Bajamal, Abdul Hafid, Bashford, Tom, Biluts, Hagos, Budohoski, Karol, Ercole, Ari, Fernández-Méndez, Rocío, Figaji, Anthony, Gupta, Deepak Kumar, Härtl, Roger, Iaccarino, Corrado, Khan, Tariq, Rubiano, Andrés, Shabani, Hamisi K, Sichizya, Kachinga, Tewari, Manoj, Tirsit, Abenezer, Thu, Myat, Tripathi, Manjul, Trivedi, Rikin, Devi, Bhagavatula Indira, Servadei, Franco, Menon, David, Kolias, Angelos, and Hutchinson, Peter

Published
2022
Full Text
View/download PDF

6. Characterizing the Impact of Using Features Extracted from Pre-trained Models on the Quality of Video Captioning Sequence-to-Sequence Models

Author

Hammad, Menatallh, Hammad, May, Elshenawy, Mohamed, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Woeginger, Gerhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Lu, Yue, editor, Vincent, Nicole, editor, Yuen, Pong Chi, editor, Zheng, Wei-Shi, editor, Cheriet, Farida, editor, and Suen, Ching Y., editor

Published
2020
Full Text
View/download PDF

9. Cryo-EM shows how dynactin recruits two dyneins for faster movement

Author

Urnavicius, Linas, Lau, Clinton K., Elshenawy, Mohamed M., Morales-Rios, Edgar, Motz, Carina, Yildiz, Ahmet, and Carter, Andrew P.

Subjects
Dynein -- Physiological aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Dynein and its cofactor dynactin form a highly processive microtubule motor in the presence of an activating adaptor, such as BICD2. Different adaptors link dynein and dynactin to distinct cargoes. Here we use electron microscopy and single-molecule studies to show that adaptors can recruit a second dynein to dynactin. Whereas BICD2 is biased towards recruiting a single dynein, the adaptors BICDR1 and HOOK3 predominantly recruit two dyneins. We find that the shift towards a double dynein complex increases both the force and speed of the microtubule motor. Our 3.5 resolution cryo-electron microscopy reconstruction of a dynein taildynactinBICDR1 complex reveals how dynactin can act as a scaffold to coordinate two dyneins side-by-side. Our work provides a structural basis for understanding how diverse adaptors recruit different numbers of dyneins and regulate the motile properties of the dyneindynactin transport machine., Author(s): Linas Urnavicius [1]; Clinton K. Lau [1]; Mohamed M. Elshenawy [2]; Edgar Morales-Rios [3]; Carina Motz [1]; Ahmet Yildiz [2, 4]; Andrew P. Carter (corresponding author) [1] Cytoplasmic dynein-1 [...]

Published
2018
Full Text
View/download PDF

11. Real-time dynamic single-molecule protein sequencing on an integrated semiconductor device

Author

Reed, Brian D., primary, Meyer, Michael J., additional, Abramzon, Valentin, additional, Ad, Omer, additional, Adcock, Pat, additional, Ahmad, Faisal R., additional, Alppay, Gün, additional, Ball, James A., additional, Beach, James, additional, Belhachemi, Dominique, additional, Bellofiore, Anthony, additional, Bellos, Michael, additional, Beltrán, Juan Felipe, additional, Betts, Andrew, additional, Bhuiya, Mohammad Wadud, additional, Blacklock, Kristin, additional, Boer, Robert, additional, Boisvert, David, additional, Brault, Norman D., additional, Buxbaum, Aaron, additional, Caprio, Steve, additional, Choi, Changhoon, additional, Christian, Thomas D., additional, Clancy, Robert, additional, Clark, Joseph, additional, Connolly, Thomas, additional, Croce, Kathren Fink, additional, Cullen, Richard, additional, Davey, Mel, additional, Davidson, Jack, additional, Elshenawy, Mohamed M., additional, Ferrigno, Michael, additional, Frier, Daniel, additional, Gudipati, Saketh, additional, Hamill, Stephanie, additional, He, Zhaoyu, additional, Hosali, Sharath, additional, Huang, Haidong, additional, Huang, Le, additional, Kabiri, Ali, additional, Kriger, Gennadiy, additional, Lathrop, Brittany, additional, Li, An, additional, Lim, Peter, additional, Liu, Stephen, additional, Luo, Feixiang, additional, Lv, Caixia, additional, Ma, Xiaoxiao, additional, McCormack, Evan, additional, Millham, Michele, additional, Nani, Roger, additional, Pandey, Manjula, additional, Parillo, John, additional, Patel, Gayatri, additional, Pike, Douglas H., additional, Preston, Kyle, additional, Pichard-Kostuch, Adeline, additional, Rearick, Kyle, additional, Rearick, Todd, additional, Ribezzi-Crivellari, Marco, additional, Schmid, Gerard, additional, Schultz, Jonathan, additional, Shi, Xinghua, additional, Singh, Badri, additional, Srivastava, Nikita, additional, Stewman, Shannon F., additional, Thurston, TR, additional, Thurston, T. R., additional, Trioli, Philip, additional, Tullman, Jennifer, additional, Wang, Xin, additional, Wang, Yen-Chih, additional, Webster, Eric A. G., additional, Zhang, Zhizhuo, additional, Zuniga, Jorge, additional, Patel, Smita S., additional, Griffiths, Andrew D., additional, van Oijen, Antoine M., additional, McKenna, Michael, additional, Dyer, Matthew D., additional, and Rothberg, Jonathan M., additional

Published
2022
Full Text
View/download PDF

12. Lis1 activates dynein motility by modulating its pairing with dynactin.

Author

Elshenawy, Mohamed M, Elshenawy, Mohamed M, Kusakci, Emre, Volz, Sara, Baumbach, Janina, Bullock, Simon L, Yildiz, Ahmet, Elshenawy, Mohamed M, Elshenawy, Mohamed M, Kusakci, Emre, Volz, Sara, Baumbach, Janina, Bullock, Simon L, and Yildiz, Ahmet

Abstract

Lissencephaly-1 (Lis1) is a key cofactor for dynein-mediated intracellular transport towards the minus-ends of microtubules. It remains unclear whether Lis1 serves as an inhibitor or an activator of mammalian dynein motility. Here we use single-molecule imaging and optical trapping to show that Lis1 does not directly alter the stepping and force production of individual dynein motors assembled with dynactin and a cargo adaptor. Instead, Lis1 promotes the formation of an active complex with dynactin. Lis1 also favours the recruitment of two dyneins to dynactin, resulting in increased velocity, higher force production and more effective competition against kinesin in a tug-of-war. Lis1 dissociates from motile complexes, indicating that its primary role is to orchestrate the assembly of the transport machinery. We propose that Lis1 binding releases dynein from its autoinhibited state, which provides a mechanistic explanation for why Lis1 is required for efficient transport of many dynein-associated cargos in cells.

Published
2020

13. Replisome speed determines the efficiency of the Tus--Ter replication termination barrier

Author

Elshenawy, Mohamed M., Jergic, Slobodan, Xu, Zhi-Qiang, Sobhy, Mohamed A., Takahashi, Masateru, Oakley, Aaron J., Dixon, Nicholas E., and Hamdan, Samir M.

Subjects
DNA synthesis -- Analysis, Chromosome replication -- Analysis, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

In all domains of life, DNA synthesis occurs bidirectionally from replication origins. Despite variable rates of replication fork progression, fork convergence often occurs at specific sites (1). Escherichia coli sets a 'replication fork trap' that allows the first arriving fork to enter but not to leave the terminus region (2-5). The trap is set by oppositely oriented Tus-bound Ter sites that block forks on approach from only one direction (3-7). However, the efficiency of fork blockage by Tus-Ter does not exceed 50% in vivo despite its apparent ability to almost permanently arrest replication forks in vitro (8, 9). Here we use data from single-molecule DNA replication assays and structural studies to show that both polarity and fork-arrest efficiency are determined by a competition between rates of Tus displacement and rearrangement of Tus-Ter interactions that leads to blockage of slower moving replisomes by two distinct mechanisms. To our knowledge this is the first example where intrinsic differences in rates of individual replisomes have different biological outcomes., In the circular E. coli chromosome, two replication forks move from the replication origin to converge opposite in a region that contains ten 23-base-pair Ter (termination) sites and the dif [...]

Published
2015

14. Casemix, management, and mortality of patients receiving emergency neurosurgery for traumatic brain injury in the Global Neurotrauma Outcomes Study: a prospective observational cohort study

Author

Clark, David, primary, Joannides, Alexis, additional, Adeleye, Amos Olufemi, additional, Bajamal, Abdul Hafid, additional, Bashford, Tom, additional, Biluts, Hagos, additional, Budohoski, Karol, additional, Ercole, Ari, additional, Fernández-Méndez, Rocío, additional, Figaji, Anthony, additional, Gupta, Deepak Kumar, additional, Härtl, Roger, additional, Iaccarino, Corrado, additional, Khan, Tariq, additional, Laeke, Tsegazeab, additional, Rubiano, Andrés, additional, Shabani, Hamisi K, additional, Sichizya, Kachinga, additional, Tewari, Manoj, additional, Tirsit, Abenezer, additional, Thu, Myat, additional, Tripathi, Manjul, additional, Trivedi, Rikin, additional, Devi, Bhagavatula Indira, additional, Servadei, Franco, additional, Menon, David, additional, Kolias, Angelos, additional, Hutchinson, Peter, additional, Abbas, Ghayur, additional, Abdallah, Omar Ibrahim, additional, Abdel-Lateef, Ahmed, additional, Abdifatah, Khalif, additional, Abdullateef, Awfa, additional, Abeygunaratne, Ruvini, additional, Aboellil, Mostafa, additional, Adam, Abass, additional, Adams, Robert, additional, Adeleye, Amos, additional, Adeolu, Augustine, additional, Adji, Novan Krisno, additional, Afianti, Nur, additional, Agarwal, Sudarsan, additional, Aghadi, Ifeanyi Kene, additional, Aguilar, Paúl Martín Méndez, additional, Ahmad, Syeda Rida, additional, Ahmed, Daniyal, additional, Ahmed, Nafees, additional, Aizaz, Haider, additional, Aji, Yunus Kuntawi, additional, Alamri, Alex, additional, Alberto, Augusto Jacinto Mussindo, additional, Alcocer, Luis Alcocer, additional, Alfaro, Lesly Gonzales, additional, Al-Habib, Amro, additional, Alhourani, Ahmad, additional, Ali, Syed Muhammad Rafay, additional, Alkherayf, Fahad, additional, AlMenabbawy, Ahmed, additional, Alshareef, Aliyah, additional, Aminullah, Muhammad Adil s/o, additional, Amjad, Madeha, additional, Amorim, Robson Luis Oliveira de, additional, Anbazhagan, Sathiaprabhu, additional, Andrade, Almir, additional, Antar, Waleed, additional, Anyomih, Theophilus T.K., additional, Aoun, Salah, additional, Apriawan, Tedy, additional, Armocida, Daniele, additional, Arnold, Paul, additional, Arraez, Miguel, additional, Assefa, Temesgen, additional, Asser, Andres, additional, Athiththan, S.P., additional, Attanayake, Deepal, additional, Aung, Maung Maung, additional, Avi, Allan, additional, Ayala, Victor Enrique Antolinez, additional, Azab, Mohammed, additional, Azam, Gaousul, additional, Azharuddin, Mohd, additional, Badejo, Olukemi, additional, Badran, Mohamed, additional, Baig, Azam Ali, additional, Baig, Rehman Ali, additional, Bajaj, Ankur, additional, Baker, Paul, additional, Bala, Renu, additional, Balasa, Artur, additional, Balchin, Ross, additional, Balogun, James, additional, Ban, Vin Shen, additional, Bandi, Bharath Kumar Reddy, additional, Bandyopadhyay, Soham, additional, Bank, Matthew, additional, Barthelemy, Ernest, additional, Bashir, Mohammed Talha, additional, Basso, Luciano Silveira, additional, Basu, Surajit, additional, Batista, Auricelio, additional, Bauer, Marlies, additional, Bavishi, Devi, additional, Beane, Abi, additional, Bejell, Shmuel, additional, Belachew, Anteneh, additional, Belli, Antonio, additional, Belouaer, Amani, additional, Bendahane, Najia El Abbadi, additional, Benjamin, Okanga, additional, Benslimane, Youssef, additional, Benyaiche, Chaymae, additional, Bernucci, Claudio, additional, Berra, Luigi Valentino, additional, Bhebe, Arnold, additional, Bimpis, Alexios, additional, Blanaru, Diana, additional, Bonfim, Jean Claude, additional, Borba, Luis A B, additional, Borcek, Alp Ozgun, additional, Borotto, Erika, additional, Bouhuwaish, Ahmad Elmabri Mohammad, additional, Bourilhon, Facundo, additional, Brachini, Gioia, additional, Breedon, Joshua, additional, Broger, Maximilian, additional, Brunetto, Giacoma Maria Floriana, additional, Bruzzaniti, Placido, additional, Budohoska, Natalia, additional, Burhan, Hira, additional, Calatroni, Maximiliano Luis, additional, Camargo, Catherine, additional, Cappai, Pier Francesco, additional, Cardali, Salvatore Massimiliano, additional, Castaño-Leon, Ana M, additional, Cederberg, David, additional, Celaya, Mikel, additional, Cenzato, Marco, additional, Challa, Lakshmi Madhavi, additional, Charest, Dhanny, additional, Chaurasia, Bipin, additional, Chenna, Rabah, additional, Cherian, Iype, additional, Ching'o, Juliana Henry, additional, Chotai, Tejas, additional, Choudhary, Ajay, additional, Choudhary, Nabeel, additional, Choumin, Florence, additional, Cigic, Tomislav, additional, Ciro, Juan, additional, Conti, Carlo, additional, Corrêa, Antônio Carlos de Souza, additional, Cossu, Giulia, additional, Couto, Maíra Piani, additional, Cruz, Aurora, additional, D'Silva, Divya, additional, D'Aliberti, Giuseppe Antonio, additional, Dampha, Lamin, additional, Daniel, Roy Thomas, additional, Dapaah, Andrew, additional, Darbar, Aneela, additional, Dascalu, Gabriel, additional, Dauda, Happy Amos, additional, Davies, Owain, additional, Delgado-Babiano, Andrea, additional, Dengl, Markus, additional, Despotovic, Marko, additional, Devi, Indira, additional, Dias, Celeste, additional, Dirar, Mohamed, additional, Dissanayake, Melina, additional, Djimbaye, Hananiah, additional, Dockrell, Simon, additional, Dolachee, Ali, additional, Dolgopolova, Julija, additional, Dolgun, Muge, additional, Dow, Abdalrouf, additional, Drusiani, Davide, additional, Dugan, Artjom, additional, Duong, Dinh Tuan, additional, Duong, Trung Kien, additional, Dziedzic, Tomasz, additional, Ebrahim, Ali, additional, El Fatemi, Nizar, additional, El Helou, Antonios El, additional, El Maaqili, Rachid El, additional, El Mostarchid, Brahim El, additional, El Ouahabi, Abdessamad El, additional, Elbaroody, Mohammad, additional, El-Fiki, Ahmed, additional, El-Garci, Ahmed, additional, El-Ghandour, Nasser M.F., additional, Elhadi, Muhammed, additional, Elleder, Vanessa, additional, Elrais, Safa, additional, El-shazly, Mohamed, additional, Elshenawy, Mohamed, additional, Elshitany, Hesham, additional, El-Sobky, Omar, additional, Emhamed, Marwa, additional, Enicker, Basil, additional, Erdogan, Onur, additional, Ertl, Sebastian, additional, Esene, Ignatius, additional, Espinosa, Omar Ocampo, additional, Fadalla, Tarig, additional, Fadelalla, Mohammed, additional, Faleiro, Rodrigo Moreira, additional, Fatima, Nida, additional, Fawaz, Charbel, additional, Fentaw, Assefa, additional, Fernandez, Carla Eiriz, additional, Ferreira, Ana, additional, Ferri, Francesco, additional, Figaji, Tony, additional, Filho, Emerson L B, additional, Fin, Loic, additional, Fisher, Benjamin, additional, Fitra, Fitra, additional, Flores, Alexis Palpan, additional, Florian, Ioan Stefan, additional, Fontana, Vincenzo, additional, Ford, Lauren, additional, Fountain, Daniel, additional, Frade, Jose Maria Roda, additional, Fratto, Antonio, additional, Freyschlag, Christian, additional, Gabin, Aranzazu Sánchez, additional, Gallagher, Clare, additional, Ganau, Mario, additional, Gandia-Gonzalez, Maria Luisa, additional, Garcia, Andoni, additional, Garcia, Borja Hernandez, additional, Garusinghe, Sanjeewa, additional, Gebreegziabher, Biniam, additional, Gelb, Adrian, additional, George, Jerome St, additional, Germanò, Antonino Francesco, additional, Ghetti, Ilaria, additional, Ghimire, Prajwal, additional, Giammarusti, Alessandro, additional, Gil, Jose Luis, additional, Gkolia, Panagiota, additional, Godebo, Yoseph, additional, Gollapudi, Prakash Rao, additional, Golubovic, Jagos, additional, Gomes, Jeremias Fernando, additional, Gonzales, Javier, additional, Gormley, William, additional, Gots, Alexander, additional, Gribaudi, Giulia Letizia, additional, Griswold, Dylan, additional, Gritti, Paolo, additional, Grobler, Ruan, additional, Gunawan, Rudy, additional, Hailemichael, Birhanu, additional, Hakkou, Elmehdi, additional, Haley, Mark, additional, Hamdan, Alhafidz, additional, Hammed, Ali, additional, Hamouda, Waeel, additional, Hamzah, Nurul Ashikin, additional, Han, Nyein Latt, additional, Hanalioglu, Sahin, additional, Haniffa, Rashan, additional, Hanko, Martin, additional, Hanrahan, John, additional, Hardcastle, Timothy, additional, Hassani, Fahd Derkaoui, additional, Heidecke, Volkmar, additional, Helseth, Eirik, additional, Hernández-Hernández, Miguel Ángel, additional, Hickman, Zachary, additional, Hoang, Le Minh Chau, additional, Hollinger, Alexa, additional, Horakova, Lenka, additional, Hossain-Ibrahim, Kismet, additional, Hou, Boru, additional, Hoz, Samer, additional, Hsu, Janine, additional, Hunn, Martin, additional, Hussain, Madiha, additional, Iacopino, Giorgia, additional, Ideta, Mylena Miki Lopes, additional, Iglesias, Irene, additional, Ilunga, Ali, additional, Imtiaz, Nafiz, additional, Islam, Rafiza, additional, Ivashchenko, Serge, additional, Izirouel, Karim, additional, Jabal, Mohamed Sobhi, additional, Jabal, Soubhi, additional, Jabang, John Nute, additional, Jamjoom, Aimun, additional, Jan, Irfan, additional, Jarju, Landing BM, additional, Javed, Saad, additional, Jelaca, Bojan, additional, Jhawar, Sukhdeep Singh, additional, Jiang, Ting Ting, additional, Jimenez, Fernando, additional, Jiris, Jorge, additional, Jithoo, Ron, additional, Johnson, Walt, additional, Joseph, Mathew, additional, Joshi, Rameshman, additional, Junttila, Eija, additional, Jusabani, Mubashir, additional, Kache, Stephen Akau, additional, Kadali, Satyavara Prasad, additional, Kalkmann, Gabriela F, additional, Kamboh, Usman, additional, Kandel, Hitham, additional, Karakus, Ahmet Kamil, additional, Kassa, Mengistu, additional, Katila, Ari, additional, Kato, Yoko, additional, Keba, Martin, additional, Kehoe, Kristy, additional, Kertmen, Huseyin Hayri, additional, Khafaji, Soha, additional, Khajanchi, Monty, additional, Khan, Mohammed, additional, Khan, Muhammad Mukhtar, additional, Khan, Sohail Daud, additional, Khizar, Ahtesham, additional, Khriesh, Amir, additional, Kierońska, Sara, additional, Kisanga, Paul, additional, Kivevele, Boniface, additional, Koczyk, Kacper, additional, Koerling, Anna-Lucia, additional, Koffenberger, Danielle, additional, Kõiv, Kennet, additional, Kõiv, Leho, additional, Kolarovszki, Branislav, additional, König, Marton, additional, Könü-Leblebicioglu, Dilek, additional, Koppala, Santhoshi Devi, additional, Korhonen, Tommi, additional, Kostkiewicz, Boguslaw, additional, Kostyra, Kacper, additional, Kotakadira, Srinivas, additional, Kotha, Arjun Reddy, additional, Kottakki, Madhu Narayana Rao, additional, Krajcinovic, Nenad, additional, Krakowiak, Michal, additional, Kramer, Andreas, additional, Krishnamoorthy, Selvamuthukumaran, additional, Kumar, Ashok, additional, Kumar, Pankaj, additional, Kumar, Pradhumna, additional, Kumarasinghe, Nilaksha, additional, Kuncha, Gowtham, additional, Kutty, Raja K., additional, Lafta, Ghazwan, additional, Lammy, Simon, additional, Lapolla, Pierfrancesco, additional, Lardani, Jacopo, additional, Lasica, Nebojsa, additional, Lastrucci, Giancarlo, additional, Launey, Yoann, additional, Lavalle, Laura, additional, Lawrence, Tim, additional, Lazaro, Albert, additional, Lebed, Vitalii, additional, Leinonen, Ville, additional, Lemeri, Lawrence, additional, Levi, Leon, additional, Lim, Jia Yi, additional, Lim, Xiao Yi, additional, Linares-Torres, Jorge, additional, Lippa, Laura, additional, Lisboa, Lurdes, additional, Liu, Jinfang, additional, Liu, Ziyuan, additional, Lo, William B, additional, Lodin, Jan, additional, Loi, Federico, additional, Londono, Daniella, additional, Lopez, Pedro Antonio Gomez, additional, López, Cristina Barceló, additional, Lotbiniere-Bassett, Madeleine De, additional, Lulens, Rihards, additional, Luna, Facundo Hector, additional, Luoto, Teemu, additional, M.V., Vijaya Sekhar, additional, Mabovula, Ndyebo, additional, MacAllister, Matthew, additional, Macie, Alcina Americo, additional, Maduri, Rodolfo, additional, Mahfoud, Moufid, additional, Mahmood, Ashraf, additional, Mahmoud, Fathia, additional, Mahoney, Dominic, additional, Makhlouf, Wissam, additional, Malcolm, George, additional, Malomo, Adefolarin, additional, Malomo, Toluyemi, additional, Mani, Manoranjitha Kumari, additional, Marçal, Tomás Gazzinelli, additional, Marchello, Jacopo, additional, Marchesini, Nicolò, additional, Marhold, Franz, additional, Marklund, Niklas, additional, Martín-Láez, Rubén, additional, Mathaneswaran, Vickneswaran, additional, Mato-Mañas, David José, additional, Maye, Helen, additional, McLean, Aaron Lawson, additional, McMahon, Catherine, additional, Mediratta, Saniya, additional, Mehboob, Mehreen, additional, Meneses, Alisson, additional, Mentri, Nesrine, additional, Mersha, Hagos, additional, Mesa, Ana Milena, additional, Meyer, Cristy, additional, Millward, Christopher, additional, Mimbir, Salomao Amone, additional, Mingoli, Andrea, additional, Mishra, Parashruram, additional, Mishra, Tejesh, additional, Misra, Basant, additional, Mittal, Siddharth, additional, Mohammed, Imran, additional, Moldovan, Ioana, additional, Molefe, Masechaba, additional, Moles, Alexis, additional, Moodley, Preston, additional, Morales, Mario Augusto Narváez, additional, Morgan, Lucy, additional, Morillo, German Del Castillo, additional, Moustafa, Wahab, additional, Moustakis, Nikolaos, additional, Mrichi, Salma, additional, Munjal, Satya Shiva, additional, Muntaka, Abdul-Jalilu Mohammed, additional, Naicker, Denver, additional, Nakashima, Paulo E H, additional, Nandigama, Pratap Kumar, additional, Nash, Samantha, additional, Negoi, Ionut, additional, Negoita, Valetina, additional, Neupane, Samundra, additional, Nguyen, Manh Hung, additional, Niantiarno, Fajar Herbowo, additional, Noble, Abbi, additional, Nor, Mohd Arman Muhamad, additional, Nowak, Blazej, additional, Oancea, Andrei, additional, O'Brien, Frazer, additional, Okere, Oghenekevwe, additional, Olaya, Sandra, additional, Oliveira, Leandro, additional, Oliveira, Louise Makarem, additional, Omar, Fatma, additional, Ononeme, Okezi, additional, Opšenák, René, additional, Orlandini, Simone, additional, Osama, Alrobah, additional, Osei-Poku, Dorcas, additional, Osman, Haytham, additional, Otero, Alvaro, additional, Ottenhausen, Malte, additional, Otzri, Shuli, additional, Outani, Oumaima, additional, Owusu, Emmanuel Abem, additional, Owusu-Agyemang, Kevin, additional, Ozair, Ahmad, additional, Ozoner, Baris, additional, Paal, Elli, additional, Paiva, Mauro Sérgio, additional, Paiva, Wellingson, additional, Pandey, Sharad, additional, Pansini, Gastone, additional, Pansini, Luigi, additional, Pantel, Tobias, additional, Pantelas, Nikolaos, additional, Papadopoulos, Konstantinos, additional, Papic, Vladimir, additional, Park, Kee, additional, Park, Nick, additional, Paschoal, Eric Homero Albuquerque, additional, Paschoalino, Mylla Christie de Oliveira, additional, Pathi, Rajesh, additional, Peethambaran, Anilkumar, additional, Pereira, Thiago Andrade, additional, Perez, Irene Panero, additional, Pérez, Claudio José Piqueras, additional, Periyasamy, Tamilanandh, additional, Peron, Stefano, additional, Phillips, Michael, additional, Picazo, Sofía Sotos, additional, Pinar, Ertugrul, additional, Pinggera, Daniel, additional, Piper, Rory, additional, Pirakash, Pathmanesan, additional, Popadic, Branko, additional, Posti, Jussi P., additional, Prabhakar, Rajmohan Bhanu, additional, Pradeepan, Sivanesalingam, additional, Prasad, Manjunath, additional, Prieto, Paola Calvachi, additional, Prince, Ron, additional, Prontera, Andrea, additional, Provaznikova, Eva, additional, Quadros, Danilo, additional, Quintero, Nezly Jadid Romero, additional, Qureshi, Mahmood, additional, Rabiel, Happiness, additional, Rada, Gabriel, additional, Ragavan, Sivagnanam, additional, Rahman, Jueria, additional, Ramadhan, Omar, additional, Ramaswamy, Padma, additional, Rashid, Sakina, additional, Rathugamage, Jagath, additional, Rätsep, Tõnu, additional, Rauhala, Minna, additional, Raza, Asif, additional, Reddycherla, Naga Raju, additional, Reen, Linus, additional, Refaat, Mohamed, additional, Regli, Luca, additional, Ren, Haijun, additional, Ria, Antonio, additional, Ribeiro, Thales Francisco, additional, Ricci, Alessandro, additional, Richterová, Romana, additional, Ringel, Florian, additional, Robertson, Faith, additional, Rocha, Catarina Mayrink Siqueira Cabral, additional, Rogério, Juvenal de Souza, additional, Romano, Adan Anibal, additional, Rothemeyer, Sally, additional, Rousseau, Gail Rousseau Gail, additional, Roza, Ranette, additional, Rueda, Kevin David Farelo, additional, Ruiz, Raiza, additional, Rundgren, Malin, additional, Rzeplinski, Radoslaw, additional, S.Chandran, Raj, additional, Sadayandi, Ramesh Andi, additional, Sage, William, additional, Sagerer, André Norbert Josef, additional, Sakar, Mustafa, additional, Salami, Mohcine, additional, Sale, Danjuma, additional, Saleh, Youssuf, additional, Sánchez-Viguera, Cristina, additional, Sandila, Saning'o, additional, Sanli, Ahmet Metin, additional, Santi, Laura, additional, Santoro, Antonio, additional, Santos, Aieska Kellen Dantas Dos, additional, Santos, Samir Cezimbra dos, additional, Sanz, Borja, additional, Sapkota, Shabal, additional, Sasidharan, Gopalakrishnan, additional, Sasillo, Ibrahim, additional, Satoskar, Rajeev, additional, Sayar, Ali Caner, additional, Sayee, Vignesh, additional, Scheichel, Florian, additional, Schiavo, Felipe Lourenzon, additional, Schupper, Alexander, additional, Schwarz, Andreas, additional, Scott, Teresa, additional, Seeberger, Esther, additional, Segundo, Claudionor Nogueira Costa, additional, Seidu, Anwar Sadat, additional, Selfa, Antonio, additional, Selmi, Nazan Has, additional, Selvarajah, Claudiya, additional, Şengel, Necmiye, additional, Seule, Martin, additional, Severo, Luiz, additional, Shah, Purva, additional, Shahzad, Muhammad, additional, Shangase, Thobekile, additional, Sharma, Mayur, additional, Shiban, Ehab, additional, Shimber, Emnet, additional, Shokunbi, Temitayo, additional, Siddiqui, Kaynat, additional, Sieg, Emily, additional, Siegemund, Martin, additional, Sikder, Shahidur Rahman, additional, Silva, Ana Cristina Veiga, additional, Silva, Ana, additional, Silva, Pedro Alberto, additional, Singh, Deepinder, additional, Skadden, Carly, additional, Skola, Josef, additional, Skouteli, Eirini, additional, Słoniewski, Pawel, additional, Smith, Brandon, additional, Solanki, Guirish, additional, Solla, Davi Fontoura, additional, Solla, Davi, additional, Sonmez, Ozcan, additional, Sönmez, Müge, additional, Soon, Wai Cheong, additional, Stefini, Roberto, additional, Stienen, Martin Nikolaus, additional, Stoica, Bogdan, additional, Stovell, Matthew, additional, Suarez, Maria Natalia, additional, Sulaiman, Alaa, additional, Suliman, Mazin, additional, Sulistyanto, Adi, additional, Sulubulut, Şeniz, additional, Sungailaite, Sandra, additional, Surbeck, Madlen, additional, Szmuda, Tomasz, additional, Taddei, Graziano, additional, Tadele, Abraham, additional, Taher, Ahmed Saleh Ahmed, additional, Takala, Riikka, additional, Talari, Krishna Murthy, additional, Tan, Bih Huei, additional, Tariciotti, Leonardo, additional, Tarmohamed, Murad, additional, Taroua, Oumayma, additional, Tatti, Emiliano, additional, Tenovuo, Olli, additional, Tetri, Sami, additional, Thakkar, Poojan, additional, Thango, Nqobile, additional, Thatikonda, Satish Kumar, additional, Thesleff, Tuomo, additional, Thomé, Claudius, additional, Thornton, Owen, additional, Timmons, Shelly, additional, Timoteo, Eva Ercilio, additional, Tingate, Campbell, additional, Tliba, Souhil, additional, Tolias, Christos, additional, Toman, Emma, additional, Torres, Ivan, additional, Torres, Luis, additional, Touissi, Youness, additional, Touray, Musa, additional, Tropeano, Maria Pia, additional, Tsermoulas, Georgios, additional, Tsitsipanis, Christos, additional, Turkoglu, Mehmet Erhan, additional, Uçkun, Özhan Merzuk, additional, Ullman, Jamie, additional, Ungureanu, Gheorghe, additional, Urasa, Sarah, additional, Ur-Rehman, Obaid, additional, Uysal, Muhammed, additional, Vakis, Antonios, additional, Valeinis, Egils, additional, Valluru, Vaishali, additional, Vannoy, Debby, additional, Vargas, Pablo, additional, Varotsis, Phillipos, additional, Varshney, Rahul, additional, Vats, Atul, additional, Veljanoski, Damjan, additional, Venturini, Sara, additional, Verma, Abhijit, additional, Villa, Clara, additional, Villa, Genaro, additional, Villar, Sofia, additional, Villard, Erin, additional, Viruez, Antonio, additional, Voglis, Stefanos, additional, Vulekovic, Petar, additional, Wadanamby, Saman, additional, Wagner, Katherine, additional, Walshe, Rebecca, additional, Walter, Jan, additional, Waseem, Marriam, additional, Whitworth, Tony, additional, Wijeyekoon, Ruwani, additional, Williams, Adam, additional, Wilson, Mark, additional, Win, Sein, additional, Winarso, Achmad Wahib Wahju, additional, Ximenes, Abraão Wagner Pessoa, additional, Yadav, Anurag, additional, Yadav, Dipak, additional, Yakoub, Kamal Makram, additional, Yalcinkaya, Ali, additional, Yan, Guizhong, additional, Yaqoob, Eesha, additional, Yepes, Carlos, additional, Yılmaz, Ayfer Nazmiye, additional, Yishak, Betelehem, additional, Yousuf, Farhat Basheer, additional, Zahari, Muhammad Zamzuri, additional, Zakaria, Hussein, additional, Zambonin, Diego, additional, Zavatto, Luca, additional, Zebian, Bassel, additional, Zeitlberger, Anna Maria, additional, Zhang, Furong, additional, Zheng, Fengwei, additional, and Ziga, Michal, additional

Published
2022
Full Text
View/download PDF

16. Cargo adaptors regulate stepping and force generation of mammalian dynein-dynactin.

Author

Elshenawy, Mohamed, Elshenawy, Mohamed, Canty, John, Oster, Liya, Ferro, Luke, Zhou, Zhou, Blanchard, Scott, Yildiz, Ahmet, Elshenawy, Mohamed, Elshenawy, Mohamed, Canty, John, Oster, Liya, Ferro, Luke, Zhou, Zhou, Blanchard, Scott, and Yildiz, Ahmet

Abstract

Cytoplasmic dynein is an ATP-driven motor that transports intracellular cargos along microtubules. Dynein adopts an inactive conformation when not attached to a cargo, and motility is activated when dynein assembles with dynactin and a cargo adaptor. It was unclear how active dynein-dynactin complexes step along microtubules and transport cargos under tension. Using single-molecule imaging, we showed that dynein-dynactin advances by taking 8 to 32-nm steps toward the microtubule minus end with frequent sideways and backward steps. Multiple dyneins collectively bear a large amount of tension because the backward stepping rate of dynein is insensitive to load. Recruitment of two dyneins to dynactin increases the force generation and the likelihood of winning against kinesin in a tug-of-war but does not directly affect velocity. Instead, velocity is determined by cargo adaptors and tail-tail interactions between two closely packed dyneins. Our results show that cargo adaptors modulate dynein motility and force generation for a wide range of cellular functions.

Published
2019

23. Sofosbuvir‐containing regimens are safe and effective in the treatment of HCV patients with moderate to severe renal impairment

Author

Eletreby, Rasha, primary, El‐Serafy, Magdy, additional, Anees, Mahmoud, additional, Kasem, Gamal, additional, Salama, Marwa, additional, Elkhouly, Reham, additional, Hamdy, Mostafa, additional, Abdel Haleem, Hisham, additional, Kamal, Ehab, additional, Abdel‐Razek, Wael, additional, Salama, Rabab, additional, Elshenawy, Mohamed, additional, Shafeek, Ayman, additional, Hassany, Mohamed, additional, El‐Sayed, Manal H., additional, El‐Shazly, Yehia, additional, and Esmat, Gamal, additional

Published
2020
Full Text
View/download PDF

28. What is all this fuss about Tus? Comparison of recent findings from biophysical and biochemical experiments

Author

Berghuis, Bojk A, Raducanu, Vlad, Elshenawy, Mohamed M, Jergic, Slobodan, Depken, Martin, Dixon, Nicholas E, Hamdan, Samir M, Dekker, Nynke H, Berghuis, Bojk A, Raducanu, Vlad, Elshenawy, Mohamed M, Jergic, Slobodan, Depken, Martin, Dixon, Nicholas E, Hamdan, Samir M, and Dekker, Nynke H

Abstract

Synchronizing the convergence of the two-oppositely moving DNA replication machineries at specific termination sites is a tightly coordinated process in bacteria. In Escherichia coli, a "replication fork trap"-found within a chromosomal region where forks are allowed to enter but not leave-is set by the protein-DNA roadblock Tus-Ter. The exact sequence of events by which Tus-Ter blocks replisomes approaching from one direction but not the other has been the subject of controversy for many decades. Specific protein-protein interactions between the nonpermissive face of Tus and the approaching helicase were challenged by biochemical and structural studies. These studies show that it is the helicase-induced strand separation that triggers the formation of new Tus-Ter interactions at the nonpermissive face-interactions that result in a highly stable "locked" complex. This controversy recently gained renewed attention as three single-molecule-based studies scrutinized this elusive Tus-Ter mechanism-leading to new findings and refinement of existing models, but also generating new questions. Here, we discuss and compare the findings of each of the single-molecule studies to find their common ground, pinpoint the crucial differences that remain, and push the understanding of this bipartite DNA-protein system further.

Published
2018

34. Dynamic structure mediates halophilic adaptation of a DNA polymerase from the deep‐sea brines of the Red Sea

Author

Takahashi, Masateru, primary, Takahashi, Etsuko, additional, Joudeh, Luay I., additional, Marini, Monica, additional, Das, Gobind, additional, Elshenawy, Mohamed M., additional, Akal, Anastassja, additional, Sakashita, Kosuke, additional, Alam, Intikhab, additional, Tehseen, Muhammad, additional, Sobhy, Mohamed A., additional, Stingl, Ulrich, additional, Merzaban, Jasmeen S., additional, Di Fabrizio, Enzo, additional, and Hamdan, Samir M., additional

Published
2018
Full Text
View/download PDF

37. What is all this fuss about Tus?: Comparison of recent findings from biophysical and biochemical experiments

Author

Berghuis, B.A. (author), Raducanu, Vlad-Stefan (author), Elshenawy, Mohamed M. (author), Jergic, Slobodan (author), Depken, S.M. (author), Dixon, NE (author), Hamdan, Samir M. (author), Dekker, N.H. (author), Berghuis, B.A. (author), Raducanu, Vlad-Stefan (author), Elshenawy, Mohamed M. (author), Jergic, Slobodan (author), Depken, S.M. (author), Dixon, NE (author), Hamdan, Samir M. (author), and Dekker, N.H. (author)

Abstract

Synchronizing the convergence of the two-oppositely moving DNA replication machineries at specific termination sites is a tightly coordinated process in bacteria. In Escherichia coli, a “replication fork trap” – found within a chromosomal region where forks are allowed to enter but not leave – is set by the protein–DNA roadblock Tus–Ter. The exact sequence of events by which Tus–Ter blocks replisomes approaching from one direction but not the other has been the subject of controversy for many decades. Specific protein–protein interactions between the nonpermissive face of Tus and the approaching helicase were challenged by biochemical and structural studies. These studies show that it is the helicase-induced strand separation that triggers the formation of new Tus–Ter interactions at the nonpermissive face – interactions that result in a highly stable “locked” complex. This controversy recently gained renewed attention as three single-molecule-based studies scrutinized this elusive Tus–Ter mechanism – leading to new findings and refinement of existing models, but also generating new questions. Here, we discuss and compare the findings of each of the single-molecule studies to find their common ground, pinpoint the crucial differences that remain, and push the understanding of this bipartite DNA–protein system further., BN/Nynke Dekker Lab, BN/Martin Depken Lab

Published
2017
Full Text
View/download PDF

40. Green2.0

Author

Papagelis, Manos, primary, Krijnen, Thomas F., additional, Elshenawy, Mohamed, additional, Konomi, Theohar, additional, Fang, Roy, additional, and El-Diraby, Tamer, additional

Published
2016
Full Text
View/download PDF

41. Two mechanisms coordinate replication termination by the Escherichia coli Tus-Ter complex

Author

Pandey, Manjula, Elshenawy, Mohamed M, Jergic, Slobodan, Takahashi, Masateru, Dixon, Nicholas E, Hamdan, Samir M, Patel, Smita S, Pandey, Manjula, Elshenawy, Mohamed M, Jergic, Slobodan, Takahashi, Masateru, Dixon, Nicholas E, Hamdan, Samir M, and Patel, Smita S

Abstract

The Escherichia coli replication terminator protein (Tus) binds to Ter sequences to block replication forks approaching from one direction. Here, we used single molecule and transient state kinetics to study responses of the heterologous phage T7 replisome to the Tus-Ter complex. The T7 replisome was arrested at the non-permissive end of Tus-Ter in a manner that is explained by a composite mousetrap and dynamic clamp model. An unpaired C(6) that forms a lock by binding into the cytosine binding pocket of Tus was most effective in arresting the replisome and mutation of C(6) removed the barrier. Isolated helicase was also blocked at the non-permissive end, but unexpectedly the isolated polymerase was not, unless C(6) was unpaired. Instead, the polymerase was blocked at the permissive end. This indicates that the Tus-Ter mechanism is sensitive to the translocation polarity of the DNA motor. The polymerase tracking along the template strand traps the C(6) to prevent lock formation; the helicase tracking along the other strand traps the complementary G(6) to aid lock formation. Our results are consistent with the model where strand separation by the helicase unpairs the GC(6) base pair and triggers lock formation immediately before the polymerase can sequester the C(6) base.

Published
2015

42. Replisome speed determines the efficiency of the Tus-Ter replication termination barrier

Author

Elshenawy, Mohamed M, Jergic, Slobodan, Xu, Zhi-Qiang, Sobhy, Mohamed A, Takahashi, Masateru, Oakley, Aaron J, Dixon, Nicholas E, Hamdan, Samir M, Elshenawy, Mohamed M, Jergic, Slobodan, Xu, Zhi-Qiang, Sobhy, Mohamed A, Takahashi, Masateru, Oakley, Aaron J, Dixon, Nicholas E, and Hamdan, Samir M

Abstract

In all domains of life, DNA synthesis occurs bidirectionally from replication origins. Despite variable rates of replication fork progression, fork convergence often occurs at specific sites1. Escherichia coli sets a 'replication fork trap' that allows the first arriving fork to enter but not to leave the terminus region2, 3, 4, 5. The trap is set by oppositely oriented Tus-bound Ter sites that block forks on approach from only one direction3, 4, 5, 6, 7. However, the efficiency of fork blockage by Tus-Ter does not exceed 50% in vivo despite its apparent ability to almost permanently arrest replication forks in vitro8, 9. Here we use data from single-molecule DNA replication assays and structural studies to show that both polarity and fork-arrest efficiency are determined by a competition between rates of Tus displacement and rearrangement of Tus-Ter interactions that leads to blockage of slower moving replisomes by two distinct mechanisms. To our knowledge this is the first example where intrinsic differences in rates of individual replisomes have different biological outcomes.

Published
2015

44. What is all this fuss about Tus? Comparison of recent findings from biophysical and biochemical experiments.

Author

Berghuis, Bojk A., Raducanu, Vlad-Stefan, Elshenawy, Mohamed M., Jergic, Slobodan, Depken, Martin, Dixon, Nicholas E., Hamdan, Samir M., and Dekker, Nynke H.

Subjects
ESCHERICHIA coli DNA, DNA replication, PROTEIN-protein interactions, SINGLE molecules, MAGNETIC tweezers
Abstract

Synchronizing the convergence of the two-oppositely moving DNA replication machineries at specific termination sites is a tightly coordinated process in bacteria. InEscherichia coli, a “replication fork trap” – found within a chromosomal region where forks are allowed to enter but not leave – is set by the protein–DNA roadblock Tus–Ter. The exact sequence of events by which Tus–Terblocks replisomes approaching from one direction but not the other has been the subject of controversy for many decades. Specific protein–protein interactions between the nonpermissive face of Tus and the approaching helicase were challenged by biochemical and structural studies. These studies show that it is the helicase-induced strand separation that triggers the formation of new Tus–Terinteractions at the nonpermissive face – interactions that result in a highly stable “locked” complex. This controversy recently gained renewed attention as three single-molecule-based studies scrutinized this elusive Tus–Termechanism – leading to new findings and refinement of existing models, but also generating new questions. Here, we discuss and compare the findings of each of the single-molecule studies to find their common ground, pinpoint the crucial differences that remain, and push the understanding of this bipartite DNA–protein system further. [ABSTRACT FROM PUBLISHER]

Published
2018
Full Text
View/download PDF

47. Simulative comparison of parallel redundant wireless systems with OMNet++

Author

Rentschler, Markus, primary, Halawa, Hassan H., additional, Daoud, Ramez M., additional, Amer, Hassanein H., additional, ElSayed, Ahmed T., additional, Nagui, Alia H., additional, ElShenawy, Mohamed M., additional, Tawfik, Karim N., additional, ElMansoury, Mohamed, additional, Hendawy, Mostafa, additional, and ElSayed, Hany M., additional

Published
2014
Full Text
View/download PDF

48. Application of parallel redundancy in a Wi-Fi-based WNCS using OPNET

Author

Hendawy, Mostafa, primary, ElMansoury, Mohamed, additional, Tawfik, Karim N., additional, ElShenawy, Mohamed M., additional, Nagui, Alia H., additional, ElSayed, Ahmed T., additional, Halawa, Hassan H., additional, Daoud, Ramez M., additional, Amer, Hassanein H., additional, Rentschler, Markus, additional, and ElSayed, Hany M., additional

Published
2014
Full Text
View/download PDF

49. Purification and characterization of theromohalophilic chitinase producing by halophilic Aspergillus flavusisolated from Suez Gulf

Author

Beltagy, Ehab Aly, Rawway, Mohammed, Abdul-Raouf, Usama Mohamed, Elshenawy, Mohamed Ahmed, and Kelany, Mahmoud Saber

Abstract

This study aimed at production of chitinase enzyme from marine waste. Out of 27 fungal isolates, Aspergillus flavus(AUMC 13576) obtained from El-Sokhna has proved to be the most potent strain for chitinase production with activity 620.54 U/l using colloidal chitin as carbon source. The enzyme was purified consecutively by ammonium sulphate precipitation, Sephadex G-100 gel filtration column and DEAE - Cellulose A52 ion exchanger chromatographic column. The molecular weight of purified chitinase was estimated to be 30 KDa by SDS-PAGE. The purified enzyme was exposed for different properties investigations. The purified chitinase gave the highest activity (1368.8 U/l) within temperature rang (60 °C) at optimum pH 7.5 and 0.9 g/l of the substrate concentration. The kinetic measurements as Km and Vmax values of the enzyme were determined to be 0.18 g chitin/ml and 274.31 U/l, respectively. The enzyme showed thermal stability at 50 °C for 15 min. in salinity concentrations (NaCl) up to 0.8 M. Among different tested heavy metals, MnCl2and FeSO4boosted the activity positively. These results indicate the potential of mesophilic A. flavus(AUMC 13576) in the production of chitinases employing shrimp as an ideal substrate.

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results