1. Assessment of interleukin-6 and cathepsin-B gene expression in breast cancer women
- Author
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Basma A. Ibrahim, Eman S. Nagdy, Essam Nour Eldin, Alaa M. I. Khalil, and Ahmed K. El-Taher
- Subjects
CA15-3 ,Cathepsins ,Interleukins ,Mammary neoplasm ,Metastasis ,Medicine (General) ,R5-920 ,Genetics ,QH426-470 - Abstract
Abstract Background Breast cancer (BC) is the most prevalent cancer and the leading cause of cancer-related deaths in women globally. Cysteine protease cathepsin-B has been implicated in various human malignancies and is involved in malignancy progression and metastasis. This study aimed to evaluate the circulating levels of cathepsin-B, interleukin-6 (IL-6), and CA15-3, a cancer antigen, as biomarkers for tumors in women with both localized and metastatic BC. The study employed a case-control design, enrolling 108 participants categorized into three groups: healthy individuals, those with localized BC, and those with metastatic BC. The relative mRNA expression of cathepsin-B in blood samples was assessed using qRT-PCR. Additionally, serum levels of IL-6 and CA15-3 were quantified using ELISA. Results The relative mRNA expression of cathepsin-B, IL-6 levels, and CA15-3 levels were significantly higher in metastatic BC cases than in localized BC cases and the control group (p-value
- Published
- 2024
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