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This study aimed to evaluate the effect of electrical stunning on quality of broiler carcasses. Two hundred of apparently healthy broiler Ross 208, 2kg weight, 37 old day from the same flock were divided into two groups, each group of 100 broiler; the first on slaughtered without stunning while the second one was stunned in water bath stunner at 40 voltage for 10 seconds voltage. The results of this study showed that the sensory evaluation including color, texture, appearance, odor and general acceptance were7, 6, 5, 7, and 6 in non- stunned carcasses while in stunned one they were 8,8,7,7, and 7,5, respectively. The mean total bacterial counts were 4.3x105 ±1.07x105 in non stunned carcasses and 3.30 x105 ±1.01 x 105 in stunned carcasses. The good effect of electrical stunning were occurred in carcasses quality because of grade B carcasses were decreased from 15% in non- stunned to 8%in stunned carcasses due to decrease of defects of brocken bone, red wing, wing bleeding spots, from 5%, 4% and, 4% in non stunned carcasses to 1%, 0% and 1% in stunned on although increase of breast bleeding spots from 2% in no stunned carcasses to 6% in stunned one. There is no detectable effect for low voltage water bath electrical stunning on chemical composition of meat (moisture, fat, protein and ash) with increase of pH. In-addition to non-significant increase in anti-oxidants enzymes of slaughtered carcasses. From these study results, we recommend using the electrical stunning before broiler slaughter as it improves the carcasses quality through decreasing the manufacture defects as broken boned and not affects the chemical composition of carcasses meat.

Escherichia coli O 157:H7 causes outbreaks of diarrhea and hemolytic uremic syndrome which can cause acute kidney failure in human. Cattle are identified as the primary source of infection. In this study, the isolation of Escherichia coli O157: H7 was occurred through out of 100 milk samples from different localities in Egypt. The positive E.coli isolated was 45 from samples then serological identification was done to identify the serotypes of E.coli which revealed that only one sample donated to E.coli O157: H7.This only positive sample used for infection of rabbit models, which was used to evaluate the pathogenesis of E.coli O157: H7.The 10 rabbits were divided into: control group (3 rabbits) and experimental group (7 rabbits). Rabbits were inoculated by feeding tube with 109 cfu of E.coliO157: H7.Fecal samples were collected from animals on days 2, 4, 6 post inoculation to determine the shedding of the pathogen. On day 13 after inoculation, intestine, kidney and liver were collected from slaughtered animals for histopathologic examination. The intestine showed necrosis and desquamation of epithelial lining with hyperplasia and neutrophilic infiltration. Kidneys showed microthrombosis in glomeruli and hyaline casts in the lumen of renal tubules. Liver showed severe congestion with sharp vacuoles in the cytoplasm of hepatocytes giving the cell the signet ring appearance.