17 results on '"Emanuelsson, E."'
Search Results
2. Antipyrine removal by TiO 2 photocatalysis based on spinning disc reactor technology
- Author
-
Expósito, A.J., primary, Patterson, D.A., additional, Mansor, W.S.W., additional, Monteagudo, J.M., additional, Emanuelsson, E., additional, Sanmartín, I., additional, and Durán, A., additional
- Published
- 2017
- Full Text
- View/download PDF
3. Biological effects of surface active agents on marine animals
- Author
-
Swedmark, M., Braaten, B., Emanuelsson, E., and Granmo, A.
- Published
- 1971
- Full Text
- View/download PDF
4. Combined volatility and mass spectrometric measurements of biogenic secondary organic aerosol
- Author
-
Emanuelsson, E., Buchholz, A., Hallquist, M., Kiendler-Scharr, A., Mentel, T., and Spindler, C.
- Subjects
ddc:550 - Published
- 2009
5. Microbiology for chemical engineers - from macro to micro scale
- Author
-
Emanuelsson, E. A. C., Emanuelsson, M. A. E., Patterson, D. A., Castro, P. M. L., Livingston, A. G., and Veritati - Repositório Institucional da Universidade Católica Portuguesa
- Subjects
Bacteria ,Long-term ,Non-sterile ,Bioreactor ,Stability - Abstract
Recent developments in microbial techniques (such as PCR, GE, FISH) have allowed researchers to detect, identify and quantify microorganisms without the limitation of culture-dependent methods. This has given both engineers and scientists a more fundamental understanding about systems containing microorganisms. These techniques can be used to monitor bacteria in wastewater treatment systems, soil and sea, industrial fermentation, food technology, and improve floccability, etc. However, despite these techniques being readily available and relatively cheap, they are not widely used by engineers. Hence, the aim of this paper is to introduce these techniques, and their applications, to chemical engineers. Two different studies related to industrial wastewater treatment, but applicable to general microorganism systems, will be presented: (1) microbial stability of pure cultures, and (2) bioreactor population shifts during alternating operational conditions. In (1), two bioreactors, inoculated with two different pure cultures, (A) Xanthobacter aut GJ10 and (B) Bulkholderia sp JS150, degrading 1,2-dichloroethane (DCE) and monochlorobenzene (MCB), respectively, were followed over time (Emanuelsson et al ., 2005). Specific and universal 16S rRNA oligonucleotide probes were used to identify the bacteria. It was found that bioreactor (A) remained pure for 290 days, whereas bioreactor (B) became contaminated within one week. The difference in behaviour is attributed to the pathway required to degrade DCE. In (2), the stability of a bacterial strain, which was isolated on the basis of its capability to degrade 2-fluorobenzoate from contaminated soil, in three different, up-flow fixed bed reactors operated under shock loads and starvation periods, was followed by denaturing gradient gel electrophoresis (DGGE) (Emanuelsson et al ., 2006). All bioreactors were rapidly colonised by different bacteria; however, the communities remained fairly stable over time, and shifts in bacterial populations were mainly found during the starvation periods.
- Published
- 2007
6. Formation of anthropogenic secondary organic aerosol (SOA) and its influence on biogenic SOA properties
- Author
-
Emanuelsson, E. U., primary, Hallquist, M., additional, Kristensen, K., additional, Glasius, M., additional, Bohn, B., additional, Fuchs, H., additional, Kammer, B., additional, Kiendler-Scharr, A., additional, Nehr, S., additional, Rubach, F., additional, Tillmann, R., additional, Wahner, A., additional, Wu, H.-C., additional, and Mentel, Th. F., additional
- Published
- 2013
- Full Text
- View/download PDF
7. Formation of anthropogenic secondary organic aerosol (SOA) and its influence on biogenic SOA properties
- Author
-
Emanuelsson, E. U., primary, Hallquist, M., additional, Kristensen, K., additional, Glasius, M., additional, Bohn, B., additional, Fuchs, H., additional, Kammer, B., additional, Kiendler-Scharr, A., additional, Nehr, S., additional, Rubach, F., additional, Tillmann, R., additional, Wahner, A., additional, Wu, H.-C., additional, and Mentel, Th. F., additional
- Published
- 2012
- Full Text
- View/download PDF
8. Heterogeneous Lollipop-like V2O5/ZnO Array: A Promising Composite Nanostructure for Visible Light Photocatalysis
- Author
-
Zou, C. W., primary, Rao, Y. F., additional, Alyamani, A., additional, Chu, W., additional, Chen, M. J., additional, Patterson, D. A., additional, Emanuelsson, E. A. C., additional, and Gao, W., additional
- Published
- 2010
- Full Text
- View/download PDF
9. Temperature sensitive crystallization of V2O5: from amorphous film to β-V2O5nanorods
- Author
-
Zou, C. W., primary, Yan, X. D., additional, Patterson, D. A., additional, Emanuelsson, E. A. C., additional, Bian, J. M., additional, and Gao, W., additional
- Published
- 2010
- Full Text
- View/download PDF
10. Microbiology for chemical engineers—from macro to micro scale
- Author
-
Emanuelsson, E. A. C., primary, Emanuelsson, M. A. E., additional, Patterson, D. A., additional, Castro, P. M. L., additional, and Livingston, A. G., additional
- Published
- 2007
- Full Text
- View/download PDF
11. Formation of anthropogenic secondary organic aerosol (SOA) and its influence on biogenic SOA properties.
- Author
-
Emanuelsson, E. U., Hallquist, M., Kristensen, K., Glasius, M., Bohn, B., Fuchs, H., Kammer, B., Kiendler-Scharr, A., Nehr, S., Rubach, F., Tillmann, R., Wahner, A., Wu, H.-C., and Mentel, Th. F.
- Abstract
Secondary organic aerosol (SOA) formation from mixed anthropogenic and biogenic precursors has been studied exposing reaction mixtures to natural sunlight in the SAPHIR chamber in Jülich, Germany. Several experiments with exclusively anthropogenic precursors were performed to establish a relationship between yield and organic aerosol mass loading for the atmospheric relevant range of aerosol loads of 0.01 to 10 µgm
-3 . The yields (0.5-9%) were comparable to previous data and further used for the detailed evaluation of the mixed biogenic and anthropogenic experiments. For the mixed experiments a number of different oxidation schemes were addressed. The reactivity, the sequence of addition, and the amount of the precursors influenced the SOA properties. Monoterpene oxidation products, including carboxylic acids and dimer esters were identified in the aged aerosol at levels comparable to ambient air. OH radicals were measured by Laser Induced Fluorescence, which allowed for establishing relations of aerosol properties and composition to the experimental OH dose. Further- more, the OH measurements in combination with the derived yields for anthropogenic SOA enabled application of a simplified model to calculate the chemical turnover of the anthropogenic precursor and corresponding anthropogenic contribution to the mixed aerosol. The estimated anthropogenic contributions were ranging from small (≈8%) up to significant fraction (>50%) providing a suitable range to study the effect of aerosol composition on the aerosol volatility (volume fraction remaining at 343 K: 0.86-0.94). The anthropogenic aerosol had higher oxygen to carbon ratio O/C and was less volatile than the biogenic fraction. However, in order to produce significant amount of anthropogenic SOA the reaction mixtures needed a higher OH dose that also increased O/C and provided a less volatile aerosol. A strong positive correlation was found between changes in volatility and O/C with the exception during dark hours where the SOA volatility decreased while O/C did not change significantly. This change in volatility under dark conditions is likely due to chemical or morphological changes not affecting O/C. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF
12. Evidence of species succession during chlorobenzene biodegradation.
- Author
-
Baptista II, Zhou NY, Emanuelsson EA, Peeva LG, Leak DJ, Mantalaris A, and Livingston AG
- Subjects
- Biodegradation, Environmental, Cell Differentiation, Computer Simulation, Proteobacteria isolation & purification, Species Specificity, Bioreactors microbiology, Chlorobenzenes metabolism, Models, Biological, Proteobacteria cytology, Proteobacteria physiology
- Abstract
We have previously reported the disappearance of a specific strain degrading chlorobenzene from a functionally stable bioreactor. In the present work, we investigated this species succession and isolated a new dominant strain, identified as Pandoraea pnomenusa sp. strain MCB032. A specific 16S rRNA-targeted oligonucleotide probe was designed and validated to identify strain MCB032 using fluorescence in situ hybridisation (FISH). The results confirmed the presence of strain MCB032 in samples collected over time, and showed that it was primarily located within the biofilm. Denaturing gradient gel electrophoresis (DGGE) provided evidence that the species succession occurred early in the operating period. The application of these biomolecular tools highlighted the remarkable stability of this new strain during the 15 months of reactor operation. The succession was attributed to the competitive kinetic behaviour of strain MCB032, which exhibited faster growth (micro(max) = 0.34 h(-1)) and higher substrate affinity (K(s) = 0.35 mg L(-1)) than strain JS150. Finally, this study contributed to the characterisation of the recently established Pandoraea genus, an emerging group in the biodegradation field., ((c) 2007 Wiley Periodicals, Inc.)
- Published
- 2008
- Full Text
- View/download PDF
13. Strain stability in biological systems treating recalcitrant organic compounds.
- Author
-
Emanuelsson EA, Baptista II, Mantalaris A, and Livingston AG
- Subjects
- Biotransformation, Burkholderia genetics, DNA Probes genetics, RNA, Ribosomal, 16S genetics, Xanthobacter genetics, Bioreactors, Burkholderia growth & development, Chlorobenzenes metabolism, Ethylene Dichlorides metabolism, Waste Disposal, Fluid, Xanthobacter growth & development
- Abstract
The availability of molecular probing technology in recent years has facilitated investigation of microbial community composition during bio-treatment of organic wastes. Particularly, it has allowed the study of microbial culture stability and correlation between stability and treatment performance. However, most studies to date have only addressed mixed cultures and there is limited information regarding single strain stability. Here we have investigated the microbial community dynamics in two bioreactors, each inoculated with a pure bacterial strain capable of degrading a recalcitrant substrate, namely Xanthobacter aut. GJ10 degrading 1,2-dichloroethane (DCE) and Burkholderia sp. JS150 degrading monochlorobenzene (MCB). Universal and strain specific 16S rRNA oligonucleotide probes were designed and used to follow strain stability. The bioreactor fed with DCE was functionally stable and the percentage of GJ10 cells in the community remained high (around 95% of total cells) throughout, even after introduction of foreign microorganisms. The bioreactor fed with MCB was also functionally stable, but in contrast to the DCE bioreactor, probing results revealed the disappearance of strain JS150 from the bioreactor within a week. The difference in behavior between the two systems is attributed to the specific pathway required to degrade DCE., (Copyright 2005 Wiley Periodicals, Inc)
- Published
- 2005
- Full Text
- View/download PDF
14. Mantle cell lymphomas with low levels of cyclin D1 long mRNA transcripts are highly proliferative and can be discriminated by elevated cyclin A2 and cyclin B1.
- Author
-
Sander B, Flygare J, Porwit-Macdonald A, Smith CI, Emanuelsson E, Kimby E, Liden J, and Christensson B
- Subjects
- 3' Untranslated Regions genetics, Aged, Aged, 80 and over, Alternative Splicing, Cell Division, DNA Primers, Female, Humans, Lymphoma, Mantle-Cell pathology, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction, Tumor Cells, Cultured, Cyclin D1 genetics, Lymphoma, Mantle-Cell genetics, RNA, Messenger genetics, Transcription, Genetic
- Abstract
The role of transcript variants of cyclin D1 in cancer biology is unclear. Most tumors with high levels of cyclin D1 express 2 transcripts due to alternative splicing: one full-length transcript of 4.4 kb and one short transcript of approximately 1.7 kb. The short transcript lacks part of the 3'UTR region regulating mRNA stability and has a longer half-life. In our study, the contribution of each of these mRNAs to gene expression and cell proliferation has been investigated in mantle cell lymphoma (MCL), a B cell lymphoma characterized by a specific gene translocation resulting in enhanced expression of cyclin D1. A subset of MCL tumors with low levels of the long cyclin D1 transcript (cyclin D1 3'UTR) was identified by quantitative PCR and by oligonucleotide array hybridization. This tumor-subset had 3.4-fold higher levels of the short form of cyclin D1 mRNA (p < 0.0001) and had higher expression of cyclin D1 protein. Gene expression analysis identified a number of cell-cycle regulatory genes as upregulated. There was a significant difference in frequencies of cyclin B1 (p = 0.0006) and cyclin A2 (p = 0.0006) positive cells that discriminated MCL with low cyclin D1 3'UTR from other highly proliferative MCL. Among differentially expressed genes, there was a highly upregulated gene with homology to the group of cell-cycle promoting E2F transcription partners, E2F_TDP5. Several of the upregulated genes, such as TOP2A, AURORA A and RRM2 may influence a response to therapy. Identification of MCL with low cyclin D1 3'UTR is important because it seems to be associated with shorter overall survival., ((c) 2005 Wiley-Liss, Inc.)
- Published
- 2005
- Full Text
- View/download PDF
15. Antiapoptotic role of growth factors in the myelodysplastic syndromes: concordance between in vitro and in vivo observations.
- Author
-
Tehranchi R, Fadeel B, Schmidt-Mende J, Forsblom AM, Emanuelsson E, Jadersten M, Christensson B, Hast R, Howe RB, Samuelsson J, Zhivotovsky B, and Hellström-Lindberg E
- Subjects
- Adult, Aged, Aged, 80 and over, Anemia, Refractory pathology, Anemia, Sideroblastic pathology, Bone Marrow Cells cytology, Bone Marrow Cells metabolism, Caspases metabolism, Cytochromes c metabolism, Enzyme Activation drug effects, Erythroid Precursor Cells pathology, Erythropoietin pharmacology, Glycophorins metabolism, Granulocyte Colony-Stimulating Factor pharmacology, Humans, In Vitro Techniques, Middle Aged, Monosomy, Receptors, Granulocyte Colony-Stimulating Factor metabolism, Recombinant Proteins, Trisomy, Anemia, Refractory drug therapy, Anemia, Sideroblastic drug therapy, Apoptosis drug effects, Erythroid Precursor Cells drug effects
- Abstract
Purpose: Erythroid apoptosis in low-risk myelodysplastic syndrome (MDS) maybe mediated via mitochondrial release of cytochrome c and subsequent caspase activation. In the present study, we compared the in vitro and in vivo effects of proerythroid treatment with erythropoietin + granulocyte colony-stimulating factor (G-CSF) on myelodysplastic erythropoiesis regarding apoptosis and preferential growth of clones with cytogenetic abnormalities., Experimental Design: We enrolled 15 refractory anemia (RA) and 11 refractory anemia with ringed sideroblasts (RARS), including 5q- aberration, monosomy 7, and trisomy 8, before initiation of treatment and followed nine patients after successful treatment. The effects of G-CSF and erythropoietin were assessed. The expression of G-CSF receptor (G-CSFR) was explored during erythroid maturation. The relative growth of erythroid progenitors with cytogenetic aberrations in presence of erythropoietin was investigated., Results: Significant redistribution of cytochrome c was seen before treatment at all stages of erythroid differentiation. This release was blocked by G-CSF during the whole culture period and by erythropoietin during the latter phase. Both freshly isolated glycophorin A+ bone marrow cells and intermediate erythroblasts during cultivation retained their expression of G-CSFR. Cytochrome c release and caspase activation were significantly less pronounced in progenitors obtained from successfully treated nonanemic patients and showed no further response to G-CSF in vitro. Moreover, erythropoietin significantly promoted growth of cytogenetically normal cells from 5q- patients, whereas no such effect was observed on erythroblasts from monosomy 7 or trisomy 8 patients., Conclusion: We conclude that growth factors such as erythropoietin and G-CSF can act both via inhibition of apoptosis of myelodysplastic erythroid precursors and via selection of cytogenetically normal progenitors.
- Published
- 2005
- Full Text
- View/download PDF
16. Overcoming oxygen limitations in membrane-attached biofilms--investigation of flux and diffusivity in an anoxic biofilm.
- Author
-
Emanuelsson EA and Livingston AG
- Subjects
- Diffusion, Membranes, Artificial, Oxygen metabolism, Toluene analysis, Toluene chemistry, Water Pollutants analysis, Biofilms, Bioreactors, Nitrates chemistry, Oxygen analysis, Waste Disposal, Fluid methods
- Abstract
The possibility of overcoming oxygen limitations in membrane-attached biofilms has been investigated by using nitrate as an electron acceptor instead of oxygen in an extractive membrane bioreactor (EMB) degrading toluene. The effect of nitrate concentration on toluene flux, the effective diffusivity in the biofilm and the biofilm activity has been investigated. A counter-diffusion-reaction model is also presented, describing the pollutant flux versus biofilm thickness. The toluene flux decreased with increasing biofilm thickness under excess nitrate concentrations, similar to the experiment with low nitrate. Mathematical modelling indicated that this was either due to decreasing activity, and/or different diffusivities in the biofilm. The effective diffusivity was investigated by using an inert tracer molecule. It remained constant for biofilm thicknesses up to 1.8mm, with a value twice that in water. The biofilm activity was investigated by inactivating a mature biofilm using sodium azide. The toluene flux remained the same before and after the addition of sodium azide, suggesting that the activity in the biofilm is very low. We conclude that the decreasing toluene flux with increasing biofilm thickness is due to the diffusional resistance of the inactive biofilm.
- Published
- 2004
- Full Text
- View/download PDF
17. The anoxic extractive membrane bioreactor.
- Author
-
Emanuelsson EA, Arcangeli JP, and Livingston AG
- Subjects
- Bacteria, Anaerobic physiology, Biodegradation, Environmental, Membranes, Artificial, Oxygen, Waste Disposal, Fluid, Bioreactors, Nitrates metabolism, Water Purification methods
- Abstract
The extractive membrane bioreactor (EMB) employs a dense silicone rubber membrane to selectively extract hydrophobic organic compounds from industrial wastewaters into a bioreactor in order to biodegrade them. The major drawback of the EMB is excess biofilm growth on the membrane, which limits mass transfer and creates oxygen limitations. In this work, nitrate has been used as an electron acceptor instead of oxygen. Due to the high solubility of nitrate in water, it is hypothesised that nitrate penetrates the whole biofilm, preventing the formation of inactive zones of bacteria. Four experiments have been performed with toluene as a model substrate under anoxic conditions. The effect of nitrate concentrations on the biofilm and on the toluene flux have been investigated. In addition, the production of soluble microbial products (SMPs), and bacterial hydrophobicity were studied. Under high nitrate concentrations, the performance of the anoxic EMB was stable and no biofilm was formed. The bacteria metabolised toluene, and the toluene flux remained approximately constant. Conversely, at low nitrate concentration, a decrease in pollutant flux concomitant with biofilm growth was observed. The production of SMPs increased under limiting nitrate concentrations, but the hydrophobicity of the suspended bacteria remained constant. However, the bacterial hydrophobicity of the attached cells was significantly greater than that of the suspended cells.
- Published
- 2003
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.