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3 results on '"Emerson Carlos de Almeida"'

1. Metalloproteomic Investigation of Hg-Binding Proteins in Renal Tissue of Rats Exposed to Mercury Chloride

Author

Emerson Carlos de Almeida, Victor Diego Faria, Felipe Dalmazzo Cirinêu, Maria G. A. Santiago, Beatriz Miotto, José C. S. Vieira, Camila Pereira Braga, Jiri Adamec, Ana A. H. Fernandes, Marília A. R. Buzalaf, and Pedro de Magalhães Padilha

Subjects
mercurial species, mercury bioaccumulation, mercury biomarkers, metallomics, LC-MS/MS, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

Results obtained from rat studies indicate that, even at low concentrations, mercurial species cause harmful effects on the kidneys, by inducing the nephrotic oxidative stress response. In the present work, Hg-associated proteins were identified as possible mercury-exposure biomarkers in rat kidneys exposed to low mercury chloride concentrations for 30 days (Hg-30) and 60 days (Hg-60), using metalloproteomic strategies. The renal proteomic profile was fractioned by two-dimensional electrophoresis and the mercury determinations in kidney samples, protein pellets and protein spots were performed using graphite furnace atomic absorption spectrometry. The characterization of Hg-associated protein spots and the analysis of differentially expressed proteins were performed by liquid chromatography, coupled with tandem mass spectrometry. Eleven Hg-associated protein spots with a concentration range of 79 ± 1 to 750 ± 9 mg kg−1 in the Hg-60 group were identified. The characterization and expression analyses allowed the identification of 53 proteins that were expressed only in the Hg-60 group, 13 “upregulated” proteins (p > 0.95) and 47 “downregulated” proteins (p < 0.05). Actin isoforms and hemoglobin subunits were identified in protein spots of the Hg-60 group, with mercury concentrations in the range of 138 to 750 mg kg−1, which qualifies these proteins as potential mercury-exposure biomarkers.

Published
2023
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2. Metalloproteomic approach to liver tissue of rats exposed to mercury

Author

Maria Gabriela A. Santiago, Victor Diego Faria, Felipe Dalmazzo Cirinêu, Lucas Luan de Lima Queiroz da Silva, Emerson Carlos de Almeida, Nubya Gonçalves Cavallini, José Cavalcante Souza Vieira, Ana Angélica Henrique Fernandes, Camila Pereira Braga, Luís Fabrício Zara, Marília Afonso Rabelo Buzalaf, Jiri Adamec, and Pedro de Magalhães Padilha

Subjects
Proteomics, Environmental Engineering, Health, Toxicology and Mutagenesis, Public Health, Environmental and Occupational Health, General Medicine, General Chemistry, Mercury, Pollution, Rats, BIOMARCADORES, Liver, Tandem Mass Spectrometry, Environmental Chemistry, Animals, Rats, Wistar, Chromatography, Liquid
Abstract

The aims of this study were to identify mercury-associated protein spots in the liver tissue of rats exposed to low concentrations of mercury and to elucidate the physiological and functional aspects of the proteins identified in the protein spots. Therefore, proteomic analysis of the liver tissue of Wistar rats exposed to mercury chloride (4.60 μg kg

Published
2022

3. Cultura digital no contexto educacional: Um olhar entre tendências e desafios para o século XXI

Author

Juliana de Moura Borges, Rosana Helena Nunes, Richar Nicolas Duran Andrades, Silvio Luiz Rutz da Silva, Adriana Aparecida de Lima Terçariol, Agnaldo Keiti Higuchi, Débora de Lourdes da Silva Sousa, Margareth Mellão Garcia Ribeiro, Dione Leite de Oliveira Almeida, Vinicius de Souza Resende da Silva, Rodnei Pereira, Giliardi Marinho Almeida, Krisângela do Nascimento, Fernanda Pereira da Silva, Eduardo Gasperoni de Oliveira, Elenilda Barrado, Amanda Fernandes da Fonseca, Luis Eduardo Veloso Garcia, José Guilherme Lança Rodrigues, Monica Roberta Devai Dias, Solange Maria de Oliveira Cruz, Renato Marcondes, Ingrid Santella Evaristo, Magda Elisete Cerri, Emerson Carlos de Almeida, Elisangela Aparecida Bulla Ikeshoji, and Ana Paula Bacchiega Prestes

Subjects
ComputingMilieux_COMPUTERSANDEDUCATION
Abstract

Digital Culture is conceived as all kinds of knowledge, habits, values and skills acquired by human beings that are built and shared in the digital environment. In this sense, the collection Digital Culture in the Educational Context: a view between trends and challenges for the 21st century brings relevant theoretical and empirical notes around what the National Common Curricular Base – BNCC – whose competence is to stimulate the critical use of technological resources, inserting both educators and students in pedagogical practices in order to learn and dominate the digital universe. The first part of the work is dedicated to Theoretical Approaches, bringing notes about Media Education with the pandemic period and what has impacted the educational scenario, both in student learning and in the performance of teaching professionals. Therefore, the reader is asked: If remote education is educational chloroquine? It also brings relevant considerations about Information and Communication Technologies applied to Distance Education and Hybrid Education, such as: Literacy in Mathematics, as well as the use of computers and gamification combined with education. Finally, with the Digital Universe, it brings an alert regarding the impacts of cyberbullying. Entitled Narratives of Experiences, the second part of the collection covers various teaching experiences with respect to the Digital Age. Among them, in elementary school, it brings challenges in the process of Literacy and Literacy practices and the teaching perception in relation to Specialized Educational Service. Considerations are made about various pedagogical resources in times of adversity. Among them: the Youtube channel of storytelling, collaborating with the reinvention of teachers in Elementary Education; and, in Higher Education, the relevance of Hybrid Education the joint application of Sole and the Google Classroom. In addition to the teaching experience, finally, testimony of the dilemmas and challenges of managerial activity in the school segment of Early Childhood Education are brought up

Published
2021

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