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154 results on '"Endepols, H"'

10. An analysis of the CatWalk XT and a composite score to assess neurofunctional deficits after photothrombosis in mice

Author

Baermann, J., Walter, H. L., Pikhovych, A., Endepols, H., Fink, G. R., Rueger, M. A., Schroeter, M., Baermann, J., Walter, H. L., Pikhovych, A., Endepols, H., Fink, G. R., Rueger, M. A., and Schroeter, M.

Abstract

The purpose of this study was to evaluate CatWalk's capability for assessing the functional outcome after photothrombotic stroke affecting the motor cortex of mice. Mice were tested up to 21 days after photothrombosis or sham surgery using CatWalk, and a composite score assessing functional deficits (neuroscore). The neuroscore demonstrated deficits of the contralateral forelimb for more than two weeks after stroke. There were no asymmetric or coordinative dysfunctions of limbs detected by CatWalk. However, CatWalk data revealed impairment of locomotion speed and its depending parameters for one-week after stroke in strong correlation to the neuroscore. Data suggest that the composite neuroscore allows to more sensitively and precisely specify and quantify photothrombosis-induced hemisyndromes than CatWalk.

Published
2021

21. Engineered antibodies: new possibilities for brain PET?

Author

Sehlin, D, Syvanen, S, Ballanger, B, Barthel, H, Bischof, GN, Boche, D, Boecker, H, Bohn, KP, Borghammer, P, Cross, D, Di Monte, D, Drzezga, A, Endepols, H, Giehl, K, Goedert, M, Hammes, J, Hansson, O, Herholz, K, Hoeglinger, G, Hoenig, M, Jessen, F, Klockgether, T, Lafaye, P, Lammerstma, A, Mandelkow, E, Mandelkow, E-M, Maurer, A, Mollenhauer, B, Neumaier, B, Nordberg, A, Onur, O, Reetz, K, Rodriguez-Vietez, E, Rominger, A, Rowe, J, Sabri, O, Schneider, A, Strafella, A, van Eimeren, T, Vasdev, N, Villemagne, V, Willbold, D, Sehlin, D, Syvanen, S, Ballanger, B, Barthel, H, Bischof, GN, Boche, D, Boecker, H, Bohn, KP, Borghammer, P, Cross, D, Di Monte, D, Drzezga, A, Endepols, H, Giehl, K, Goedert, M, Hammes, J, Hansson, O, Herholz, K, Hoeglinger, G, Hoenig, M, Jessen, F, Klockgether, T, Lafaye, P, Lammerstma, A, Mandelkow, E, Mandelkow, E-M, Maurer, A, Mollenhauer, B, Neumaier, B, Nordberg, A, Onur, O, Reetz, K, Rodriguez-Vietez, E, Rominger, A, Rowe, J, Sabri, O, Schneider, A, Strafella, A, van Eimeren, T, Vasdev, N, Villemagne, V, and Willbold, D

Abstract

Almost 50 million people worldwide are affected by Alzheimer's disease (AD), the most common neurodegenerative disorder. Development of disease-modifying therapies would benefit from reliable, non-invasive positron emission tomography (PET) biomarkers for early diagnosis, monitoring of disease progression, and assessment of therapeutic effects. Traditionally, PET ligands have been based on small molecules that, with the right properties, can penetrate the blood-brain barrier (BBB) and visualize targets in the brain. Recently a new class of PET ligands based on antibodies have emerged, mainly in applications related to cancer. While antibodies have advantages such as high specificity and affinity, their passage across the BBB is limited. Thus, to be used as brain PET ligands, antibodies need to be modified for active transport into the brain. Here, we review the development of radioligands based on antibodies for visualization of intrabrain targets. We focus on antibodies modified into a bispecific format, with the capacity to undergo transferrin receptor 1 (TfR1)-mediated transcytosis to enter the brain and access pathological proteins, e.g. amyloid-beta. A number of such antibody ligands have been developed, displaying differences in brain uptake, pharmacokinetics, and ability to bind and visualize the target in the brain of transgenic mice. Potential pathological changes related to neurodegeneration, e.g. misfolded proteins and neuroinflammation, are suggested as future targets for this novel type of radioligand. Challenges are also discussed, such as the temporal match of radionuclide half-life with the ligand's pharmacokinetic profile and translation to human use. In conclusion, brain PET imaging using bispecific antibodies, modified for receptor-mediated transcytosis across the BBB, is a promising method for specifically visualizing molecules in the brain that are difficult to target with traditional small molecule ligands.

Published
2019

24. P2973Glutamate carboxypeptidase II expression-dependent microPET visualization of reendothelialization after balloon denudation in a rat model: a novel tool for non-invasive in vivo molecular imaging

Author

Cornelissen, A., primary, Zlatopolskiy, B., additional, Simsekyilmaz, S., additional, Endepols, H., additional, Brucerius, J., additional, Winz, O., additional, Neumaier, A., additional, Morgenroth, A., additional, Marx, N., additional, Mottaghy, F., additional, and Vogt, F., additional

Published
2017
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26. Glutamate carboxypeptidase II expression-dependent microPET visualization of reendothelialization after balloon denudation in a rat model: a novel tool for non-invasive in vivo molecular imaging

Author

Cornelissen, A., Zlatopolskiy, B., Simsekyilmaz, S., Endepols, H., Brucerius, J., Winz, O., Neumaier, A., Morgenroth, A., Marx, N., Mottaghy, F., Vogt, F., Cornelissen, A., Zlatopolskiy, B., Simsekyilmaz, S., Endepols, H., Brucerius, J., Winz, O., Neumaier, A., Morgenroth, A., Marx, N., Mottaghy, F., and Vogt, F.

Published
2017

31. Conflict Processing in the Rat Brain: Behavioral Analysis and Functional μPET Imaging Using [18F]Fluorodeoxyglucose

Author

Marx, C., Lex, B., Calaminus, C., Hauber, W., Backes, H., Bernd Neumaier, Mies, G., Graf, R., and Endepols, H.

Subjects
Behavioral Neuroscience, prefrontal cortex, Neuropsychology and Physiological Psychology, Cognitive Neuroscience, cognitive conflict, Simon task, rodent model, Original Research
Abstract

Conflicts in spatial stimulus-response tasks occur when the task-relevant feature of a stimulus implies a response toward a certain location which does not match the location of stimulus presentation. This conflict leads to increased error rates and longer reaction times, which has been termed Simon effect. A model of dual route processing (automatic and intentional) of stimulus features has been proposed, predicting response conflicts if the two routes are incongruent. Although there is evidence that the prefrontal cortex, notably the anterior cingulate cortex (ACC), plays a crucial role in conflict processing, the neuronal basis of dual route architecture is still unknown. In this study, we pursue a novel approach using positron emission tomography (PET) to identify relevant brain areas in a rat model of an auditory Simon task, a neuropsychological interference task, which is commonly used to study conflict processing in humans. For combination with PET we used the metabolic tracer [(18)F]fluorodeoxyglucose, which accumulates in metabolically active brain cells during the behavioral task. Brain areas involved in conflict processing are supposed to be activated when automatic and intentional route processing lead to different responses (dual route model). Analysis of PET data revealed specific activation patterns for different task settings applicable to the dual route model as established for response conflict processing. The rat motor cortex (M1) may be part of the automatic route or involved in its facilitation, while premotor (M2), prelimbic, and ACC seemed to be essential for inhibiting the incorrect, automatic response, indicating conflict monitoring functions. Our findings and the remarkable similarities to the pattern of activated regions reported during conflict processing in humans demonstrate that our rodent model opens novel opportunities to investigate the anatomical basis of conflict processing and dual route architecture.

Published
2012
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43. Influence of Descending Forebrain Projections on Processing of AcousticSignals and Audiomotor Integration in the Anuran Midbrain.

Author

Endepols, H. and Walkowiak, W.

Subjects
*AUDITORY perception, *PROSENCEPHALON physiology, ANURA physiology
Abstract

We tested the role of descending projections forauditory processing and audiomotor integration in the anuran torus semicircularis.Intracellular recordings were made from isolated brain preparations, impaledneurons were stained. Auditory neurons responded to electrical stimulationof striatum and/or dorsal thalamus, they integrated forebrain and auditorynerve inputs. High frequency stimulation in striatum or thalamus changed theauditory response of torus neurons located in the laminar subnucleus. Ourresults suggest that the laminar nucleus is the primary target of forebrainprojections, which provides a basis for modulation of acoustically guidedbehaviour. [ABSTRACT FROM AUTHOR]

Published
1999
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50. Preserved striatal innervation maintains motor function despite severe loss of nigral dopaminergic neurons.

Author

Paß T, Ricke KM, Hofmann P, Chowdhury RS, Nie Y, Chinnery P, Endepols H, Neumaier B, Carvalho A, Rigoux L, Steculorum SM, Prudent J, Riemer T, Aswendt M, Liss B, Brachvogel B, and Wiesner RJ

Subjects
Animals, Mice, Mice, Transgenic, DNA, Mitochondrial genetics, Motor Activity physiology, Mutation, DNA Helicases genetics, Mitochondrial Proteins metabolism, Mitochondrial Proteins genetics, Male, Dopamine metabolism, Dopaminergic Neurons pathology, Dopaminergic Neurons metabolism, Substantia Nigra pathology, Substantia Nigra metabolism, Corpus Striatum pathology, Corpus Striatum metabolism
Abstract

Degeneration of dopaminergic neurons in the substantia nigra and their striatal axon terminals causes cardinal motor symptoms of Parkinson's disease. In idiopathic cases, high levels of mitochondrial DNA alterations, leading to mitochondrial dysfunction, are a central feature of these vulnerable neurons. Here we present a mouse model expressing the K320E variant of the mitochondrial helicase Twinkle in dopaminergic neurons, leading to accelerated mitochondrial DNA mutations. These K320E-TwinkleDaN mice showed normal motor function at 20 months of age, although ∼70% of nigral dopaminergic neurons had perished. Remaining neurons still preserved ∼75% of axon terminals in the dorsal striatum and enabled normal dopamine release. Transcriptome analysis and viral tracing confirmed compensatory axonal sprouting of the surviving neurons. We conclude that a small population of substantia nigra dopaminergic neurons is able to adapt to the accumulation of mitochondrial DNA mutations and maintain motor control., (© The Author(s) 2024. Published by Oxford University Press on behalf of the Guarantors of Brain. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

Published
2024
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