13 results on '"Enkhnaran, Bilegsaikhan"'
Search Results
2. microRNA-93-5p promotes hepatocellular carcinoma progression via a microRNA-93-5p/MAP3K2/c-Jun positive feedback circuit
- Author
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Tao-Tao Liu, Asha Balakrishnan, Xiang-Nan Yu, Ling Dong, Guang-Cong Zhang, Hong-Ying Guo, Ji-Min Zhu, Guangqi Song, Michael Ott, Jialei Sun, Hai-Rong Zhu, Xuan Shi, Xizhong Shen, Enkhnaran Bilegsaikhan, and Shuqiang Weng
- Subjects
0301 basic medicine ,Cancer Research ,Three prime untranslated region ,c-jun ,RNA activation ,MAP3K2 ,Biology ,digestive system diseases ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Downregulation and upregulation ,030220 oncology & carcinogenesis ,microRNA ,Gene expression ,Genetics ,Cancer research ,Protein kinase A ,Molecular Biology - Abstract
Cumulative evidence suggests that microRNAs (miRNAs) promote gene expression in cancers. However, the pathophysiologic relevance of miRNA-mediated RNA activation in hepatocellular carcinoma (HCC) remains to be established. Our previous miRNA expression profiling in seven-paired HCC specimens revealed miR-93-5p as an HCC-related miRNA. In this study, miR-93-5p expression was assessed in HCC tissues and cell lines by quantitative real-time PCR and fluorescence in situ hybridization. The correlation of miR-93-5p expression with survival and clinicopathological features of HCC was determined by statistical analysis. The function and potential mechanism of miR-93-5p in HCC were further investigated by a series of gain- or loss-of-function experiments in vitro and in vivo. We identified that miR-93-5p, overexpressed in HCC specimens and cell lines, leads to poor outcomes in HCC cases and promotes proliferation, migration, and invasion in HCC cell lines. Mechanistically, rather than decreasing target mRNA levels as expected, miR-93-5p binds to the 3'-untranslated region (UTR) of mitogen-activated protein kinase kinase kinase 2 (MAP3K2) to directly upregulate its expression and downstream p38 and c-Jun N-terminal kinase (JNK) pathway, thereby leading to cell cycle progression in HCC. Notably, we also demonstrated that c-Jun, a downstream effector of the JNK pathway, enhances miR-93-5p transcription by targeting its promoter region. Besides, downregulation of miR-93-5p significantly retarded tumor growth, while overexpression of miR-93-5p accelerated tumor growth in the HCC xenograft mouse model. Altogether, we revealed a miR-93-5p/MAP3K2/c-Jun positive feedback loop to promote HCC progression in vivo and in vitro, representing an RNA-activating role of miR-93-5p in HCC development.
- Published
- 2020
3. UBE2T promotes proliferation via G2/M checkpoint in hepatocellular carcinoma
- Author
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Xizhong Shen, Xiang-Nan Yu, Ji-Min Zhu, Jian Wu, Hong-Ying Guo, Enkhnaran Bilegsaikhan, Li-Li Liu, Xuan Shi, and Hai-Rong Zhu
- Subjects
0301 basic medicine ,Gene knockdown ,Cell cycle checkpoint ,Chemistry ,Kinase ,Cell cycle ,medicine.disease_cause ,digestive system diseases ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,In vivo ,030220 oncology & carcinogenesis ,medicine ,Cancer research ,Carcinogenesis ,Cyclin B1 ,Ex vivo - Abstract
Background Growing evidence suggests that the ubiquitin-proteasome system is involved in the pathogenesis and recurrence of hepatocellular carcinoma (HCC); yet, little is known about the role of ubiquitin-conjugating enzyme E2T (UBE2T) in HCC. Materials and methods UBE2T levels were detected in HCC tissues and hepatoma cell lines using quantitative reserve transcriptase-polymerase chain reaction and Western blot analysis. Next, the changes of phenotypes after UBE2T knockdown or overexpression were evaluated using in vitro methods. Finally, the mechanism of UBE2T in HCC was tested using ex vivo and in vivo methods. Results In the present study, we reported that UBE2T mRNA and protein levels were significantly upregulated in HCC tissues compared to adjacent non-tumor tissues. Additionally, suppression of UBE2T expression inhibited proliferation, colony formation, tumorigenesis, migration, and invasion of hepatoma cells, whereas UBE2T overexpression led to the opposite outcomes. Moreover, suppression of UBE2T expression resulted in an increase in G2/M phase and a decrease in the percentage of cells in G1 phase, which indicated a cell cycle arrest at the G2/M phase. In contrast, the percentage of cells in G2/M phase decreased following UBE2T overexpression. Further study indicated that UBE2T regulated the G2/M transition by modulating cyclin B1 and cyclin-dependent kinase 1. Conclusion Taken together, the findings of the present study uncover biological functions of UBE2T in hepatoma cells, and delineate preliminary molecular mechanisms of UBE2T in modulating HCC development and progression.
- Published
- 2019
4. Comprehensive analysis of long non-coding RNA-messenger RNA-microRNA co-expression network identifies cell cycle-related lncRNA in hepatocellular carcinoma
- Author
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Harry L.A. Janssen, Yu Cai, Ling Dong, Hai‑Rong Zhu, Li‑Li Liu, Jian Wu, Enkhnaran Bilegsaikhan, Xiang‑Nan Yu, Ji‑Min Zhu, Xuan Shi, Shu Qiang Weng, Hong‑Ying Guo, Xi Zhong Shen, Tao Tao Liu, Guang‑Cong Zhang, and Guang‑Qi Song
- Subjects
0301 basic medicine ,Male ,Carcinoma, Hepatocellular ,Kaplan-Meier Estimate ,Biology ,liver cancer ,03 medical and health sciences ,0302 clinical medicine ,microRNA ,Genetics ,Humans ,RNA, Messenger ,KEGG ,Gene ,Regulation of gene expression ,long non-coding RNA ,Cell Cycle ,Liver Neoplasms ,RNA ,General Medicine ,Articles ,Cell cycle ,Middle Aged ,Long non-coding RNA ,Gene expression profiling ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Gene Ontology ,030220 oncology & carcinogenesis ,Cancer research ,lncRNA-miRNA-mRNA interaction network ,RNA, Long Noncoding ,microarray - Abstract
Long non‑coding RNAs (lncRNAs) have been shown to contribute to progression and prognosis of hepatocellular carcinoma (HCC). However, expression profiling and interaction of lncRNAs with messenger RNAs (mRNAs) and microRNAs (miRNAs) remain largely unknown in HCC. The expression profiling of lncRNAs, mRNA and miRNAs was obtained using microarray. The Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis were used to characterize potential functions of differentially expressed mRNAs. Cytoscape was applied to construct an lncRNA‑miRNA‑mRNA co‑expression network and candidate lncRNAs were validated via quantitative PCR in 30 pairs of HCC and adjacent tumor‑free tissues. In this study, 1,056 upregulated and 1,288 downregulated lncRNAs were identified, while 2,687 mRNAs and 6 miRNAs were aberrantly expressed in HCC compared with adjacent tumor‑free tissues. Potential functions of differentially expressed mRNAs were demonstrated to significantly participate in modulating critical genes in the cell cycle, such as cyclin E1 and cyclin B2. After screening, 95 lncRNAs, 5 miRNAs and 36 mRNAs were recruited for construction of lncRNA‑mRNA‑miRNA co‑expression network in the cell cycle pathway. Subsequently, the top 5 lncRNAs that potentially modulate critical genes in the cell cycle were selected as the candidates for further verification. Kaplan‑Meier curves using the Cancer Genome Atlas database showed that 13 targeted mRNAs were associated with overall survival of HCC patients. Finally, three lncRNAs, including ENST00000522221, lnc‑HACE1‑6:1 and lnc‑ICOSLG‑11:1, are significantly upregulated in HCC tissues compared with adjacent tumor‑free tissues. These findings suggest that lncRNAs play essential roles in the pathogenesis of HCC via regulating coding genes and miRNAs, and may be important targets for diagnosis and treatment of this disease.
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- 2019
5. Overexpressed pepsinogen C is associated with poor prognosis in human hepatocellular carcinoma: a tissue microarray study
- Author
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Xiang-Nan Yu, Xuan Shi, Ren-Zheng Huang, Xizhong Shen, Enkhnaran Bilegsaikhan, Hong Chen, Hai-Rong Zhu, Tao-Tao Liu, Hong-Ying Guo, and Ji-Min Zhu
- Subjects
0301 basic medicine ,Tissue microarray ,Tumor size ,Proportional hazards model ,business.industry ,Pepsinogen C ,Subgroup analysis ,medicine.disease_cause ,medicine.disease ,digestive system diseases ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Cancer research ,Medicine ,Clinical significance ,business ,Carcinogenesis - Abstract
Background: Aberrant expression of pepsinogen C (PGC) has been observed in human cancers. However, its role in hepatocellular carcinoma (HCC) remains to be established. The goal of this study is to illustrate PGC expression and to evaluate its clinical relevance in HCC. Materials and methods: PGC expression was examined in 75 pairs of HCC and adjacent non-tumor tissues using tissue microarray. The correlations between its expression and clinical parameters were also analyzed. Results: PGC overexpression was significantly associated with larger tumor size (≥5 cm; P=0.017) and incomplete encapsulation (P
- Published
- 2019
6. microRNA-93-5p promotes hepatocellular carcinoma progression via a microRNA-93-5p/MAP3K2/c-Jun positive feedback circuit
- Author
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Xuan, Shi, Tao-Tao, Liu, Xiang-Nan, Yu, Asha, Balakrishnan, Hai-Rong, Zhu, Hong-Ying, Guo, Guang-Cong, Zhang, Enkhnaran, Bilegsaikhan, Jia-Lei, Sun, Guang-Qi, Song, Shu-Qiang, Weng, Ling, Dong, Michael, Ott, Ji-Min, Zhu, and Xi-Zhong, Shen
- Subjects
Male ,Carcinoma, Hepatocellular ,Proto-Oncogene Proteins c-jun ,Liver Neoplasms ,Mice, Nude ,Hep G2 Cells ,MAP Kinase Kinase Kinase 2 ,Transfection ,Up-Regulation ,Mice ,MicroRNAs ,Cell Movement ,Cell Line, Tumor ,Animals ,Heterografts ,Humans ,Neoplasm Invasiveness ,Phosphorylation ,Promoter Regions, Genetic ,3' Untranslated Regions ,Cell Proliferation - Abstract
Cumulative evidence suggests that microRNAs (miRNAs) promote gene expression in cancers. However, the pathophysiologic relevance of miRNA-mediated RNA activation in hepatocellular carcinoma (HCC) remains to be established. Our previous miRNA expression profiling in seven-paired HCC specimens revealed miR-93-5p as an HCC-related miRNA. In this study, miR-93-5p expression was assessed in HCC tissues and cell lines by quantitative real-time PCR and fluorescence in situ hybridization. The correlation of miR-93-5p expression with survival and clinicopathological features of HCC was determined by statistical analysis. The function and potential mechanism of miR-93-5p in HCC were further investigated by a series of gain- or loss-of-function experiments in vitro and in vivo. We identified that miR-93-5p, overexpressed in HCC specimens and cell lines, leads to poor outcomes in HCC cases and promotes proliferation, migration, and invasion in HCC cell lines. Mechanistically, rather than decreasing target mRNA levels as expected, miR-93-5p binds to the 3'-untranslated region (UTR) of mitogen-activated protein kinase kinase kinase 2 (MAP3K2) to directly upregulate its expression and downstream p38 and c-Jun N-terminal kinase (JNK) pathway, thereby leading to cell cycle progression in HCC. Notably, we also demonstrated that c-Jun, a downstream effector of the JNK pathway, enhances miR-93-5p transcription by targeting its promoter region. Besides, downregulation of miR-93-5p significantly retarded tumor growth, while overexpression of miR-93-5p accelerated tumor growth in the HCC xenograft mouse model. Altogether, we revealed a miR-93-5p/MAP3K2/c-Jun positive feedback loop to promote HCC progression in vivo and in vitro, representing an RNA-activating role of miR-93-5p in HCC development.
- Published
- 2020
7. microRNA-19a-3p promotes tumor metastasis and chemoresistance through the PTEN/Akt pathway in hepatocellular carcinoma
- Author
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Guangqi Song, Hong-Ying Guo, Xiaoxi Huang, Xiang-Nan Yu, Tao-Tao Liu, Ji-Min Zhu, Hai-Rong Zhu, Xuan Shi, Xuemei Jiang, Shuqiang Weng, Harry L.A. Janssen, Xizhong Shen, Ling Dong, and Enkhnaran Bilegsaikhan
- Subjects
0301 basic medicine ,Carcinoma, Hepatocellular ,Cell ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,microRNA ,medicine ,Humans ,PTEN ,Tensin ,Gene silencing ,neoplasms ,PI3K/AKT/mTOR pathway ,Pharmacology ,biology ,Liver Neoplasms ,PTEN Phosphohydrolase ,General Medicine ,medicine.disease ,digestive system diseases ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Hepatocytes ,biology.protein ,Cancer research ,Ectopic expression ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
microRNA-19a-3p (miR-19a-3p) has been reported to regulate cell proliferation in hepatocellular carcinoma (HCC), but its role in HCC metastasis remains unknown. In this study, miR-19a-3p was noted to be upregulated in HCC specimens and cell lines. Aberrant expression of miR-19a-3p stimulated HCC cell metastasis, and phosphatase and tensin homolog (PTEN) was shown to be a direct target of miR-19a-3p. miR-19a-3p-mediated HCC metastasis was reversed by restoration of PTEN or could be imitated by silencing of PTEN. Modulation of miR-19a-3p also altered expression of phosphorylated Akt, a downstream mediator of PTEN. Moreover, aberrant expression of miR-19a-3p induced sorafenib resistance by regulating the PTEN/Akt pathway. In conclusion, ectopic expression of miR-19a-3p contributes to HCC metastasis and chemoresistance by modulating PTEN expression and the PTEN-dependent pathways.
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- 2018
8. Circulating miR-338-5p is a potential diagnostic biomarker in colorectal cancer
- Author
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Xi Zhong Shen, Hai-Ning Liu, Enkhnaran Bilegsaikhan, and Tao Tao Liu
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Receiver operating characteristic ,biology ,Colorectal cancer ,business.industry ,Gastroenterology ,medicine.disease ,digestive system diseases ,Peripheral blood ,Fold change ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Carcinoembryonic antigen ,030220 oncology & carcinogenesis ,Internal medicine ,medicine ,biology.protein ,Diagnostic biomarker ,Clinicopathological features ,In patient ,business - Abstract
OBJECTIVE The expression of miR-338-5p has been reported to be upregulated in colorectal cancer (CRC) tissues. Clinicopathological features indicate that miR-338-5p overexpression correlates with the metastatic status of CRC. This study was aimed to investigate the diagnostic value of serum miR-338-5p for CRC. METHODS Peripheral blood samples were collected from 210 participants, including 80 patients with CRC, 50 with colorectal polyps and 80 healthy controls. Serum miR-338-5p was quantified by quantitative reverse-transcription polymerase chain reaction. The area under the receiver operating characteristic curve (AUROC) was used to estimate the diagnostic value of miR-338-5p, carcinoembryonic antigen (CEA) and the combination of these two biomarkers. RESULTS Serum miR-338-5p levels (fold change) in patients with CRC and colorectal polyps, and controls were 4.94 ± 1.13, 4.12 ± 0.75 and 3.07 ± 0.75, respectively. Significant differences were observed between the groups (P < 0.001). The AUROC of miR-338-5p was 0.923 (95% CI 0.882-0.964) and 0.845 (95% CI 0.792-0.898), respectively, for distinguishing CRC from healthy controls or from those without CRC. The AUROC of the combination of miR-338-5p and CEA was 0.932 (95% CI 0.882-0.964), with a sensitivity of 85%, a specificity of 88.8% at a cut-off value of 8.16. CONCLUSIONS Circulating miR-338-5p may serve as a potential noninvasive diagnostic biomarker for detecting CRC. The combination of miR-338-5p and CEA exhibits the highest diagnostic value in our study.
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- 2018
9. Microarray Expression Profiling of microRNAs Reveals Potential Biomarkers for Hepatocellular Carcinoma
- Author
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Xizhong Shen, Xiang-Nan Yu, Guangqi Song, Harry L.A. Janssen, Shuqiang Weng, Ren-Zheng Huang, Ji-Min Zhu, Ling Dong, Hong-Ying Guo, Hai-Rong Zhu, Xuan Shi, and Enkhnaran Bilegsaikhan
- Subjects
Adult ,Male ,0301 basic medicine ,Carcinoma, Hepatocellular ,Microarray ,Context (language use) ,Biology ,Sensitivity and Specificity ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Liver disease ,0302 clinical medicine ,microRNA ,Biomarkers, Tumor ,medicine ,Humans ,Stage (cooking) ,Aged ,Oligonucleotide Array Sequence Analysis ,Receiver operating characteristic ,Gene Expression Profiling ,Liver Neoplasms ,Reproducibility of Results ,General Medicine ,Middle Aged ,medicine.disease ,Gene Expression Regulation, Neoplastic ,Gene expression profiling ,MicroRNAs ,030104 developmental biology ,Area Under Curve ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Cancer research ,Female - Abstract
Hepatocellular carcinoma (HCC) remains a major health problem for delayed diagnosis, inefficient surveillance and poor prognosis. Recent studies have indicated that non-coding RNAs contribute to the development of new strategies for diagnosis and treatment of HCC. In the present study, we employed 18 pairs of HCC and matched non-tumor tissues for the identification of differentially expressed microRNAs (miRNAs) in HCC, among which 7 paired specimens were selected randomly for microarray detection. Totally, twenty-three miRNAs were screened out to have statistically significant differences with the threshold of P < 0.01 and fold-change ≥ 2.0 or ≤ 0.5 using miRNA microarray. In the validation stage, two miRNAs exhibited higher expression levels in the HCC tissues compared with those in the matched non-tumor tissues, whereas the expression levels of ten miRNAs were lower in the HCC tissues than those in the matched non-tumor tissues. In further analysis, eight miRNAs, including miR-4270, miR-125b-5p, miR-199a-3p, miR-10a-5p, miR-424-5p, miR-195-5p, miR-106b-5p and miR-3651, were retained, when another constraint about the signal intensity of microarray probes was established. Among these miRNAs, our study was the first to show the higher expression level of miR-3651 and the lower expression level of miR-4270 in HCC. The areas under the receiver-operating-characteristic curve values of miR-3651 and miR-4270 were 0.730 and 0.967, respectively, indicating their potential diagnostic values. Our results may help provide the context for expanded interpretations of miRNA studies involved in the progression of liver disease, potentially serving as a diagnostic tool of HCC.
- Published
- 2018
10. UBE2T promotes proliferation via G2/M checkpoint in hepatocellular carcinoma
- Author
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Li-Li, Liu, Ji-Min, Zhu, Xiang-Nan, Yu, Hai-Rong, Zhu, Xuan, Shi, Enkhnaran, Bilegsaikhan, Hong-Ying, Guo, Jian, Wu, and Xi-Zhong, Shen
- Subjects
cell cycle ,hepatocellular carcinoma ,UBE2T ,ubiquitination ,digestive system diseases ,Original Research - Abstract
Background Growing evidence suggests that the ubiquitin-proteasome system is involved in the pathogenesis and recurrence of hepatocellular carcinoma (HCC); yet, little is known about the role of ubiquitin-conjugating enzyme E2T (UBE2T) in HCC. Materials and methods UBE2T levels were detected in HCC tissues and hepatoma cell lines using quantitative reserve transcriptase-polymerase chain reaction and Western blot analysis. Next, the changes of phenotypes after UBE2T knockdown or overexpression were evaluated using in vitro methods. Finally, the mechanism of UBE2T in HCC was tested using ex vivo and in vivo methods. Results In the present study, we reported that UBE2T mRNA and protein levels were significantly upregulated in HCC tissues compared to adjacent non-tumor tissues. Additionally, suppression of UBE2T expression inhibited proliferation, colony formation, tumorigenesis, migration, and invasion of hepatoma cells, whereas UBE2T overexpression led to the opposite outcomes. Moreover, suppression of UBE2T expression resulted in an increase in G2/M phase and a decrease in the percentage of cells in G1 phase, which indicated a cell cycle arrest at the G2/M phase. In contrast, the percentage of cells in G2/M phase decreased following UBE2T overexpression. Further study indicated that UBE2T regulated the G2/M transition by modulating cyclin B1 and cyclin-dependent kinase 1. Conclusion Taken together, the findings of the present study uncover biological functions of UBE2T in hepatoma cells, and delineate preliminary molecular mechanisms of UBE2T in modulating HCC development and progression.
- Published
- 2019
11. Overexpressed pepsinogen C is associated with poor prognosis in human hepatocellular carcinoma: a tissue microarray study
- Author
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Hong, Chen, Hai-Rong, Zhu, Xiang-Nan, Yu, Xuan, Shi, Enkhnaran, Bilegsaikhan, Hong-Ying, Guo, Ren-Zheng, Huang, Tao-Tao, Liu, Xi-Zhong, Shen, and Ji-Min, Zhu
- Subjects
tissue microarray ,tumor size ,pepsinogen C ,hepatocellular carcinoma ,prognostic biomarker ,digestive system diseases ,Original Research - Abstract
Background: Aberrant expression of pepsinogen C (PGC) has been observed in human cancers. However, its role in hepatocellular carcinoma (HCC) remains to be established. The goal of this study is to illustrate PGC expression and to evaluate its clinical relevance in HCC. Materials and methods: PGC expression was examined in 75 pairs of HCC and adjacent non-tumor tissues using tissue microarray. The correlations between its expression and clinical parameters were also analyzed. Results: PGC overexpression was significantly associated with larger tumor size (≥5 cm; P=0.017) and incomplete encapsulation (P
- Published
- 2018
12. Circulating miR-338-5p is a potential diagnostic biomarker in colorectal cancer
- Author
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Enkhnaran, Bilegsaikhan, Hai Ning, Liu, Xi Zhong, Shen, and Tao Tao, Liu
- Subjects
Adult ,Male ,Colonic Polyps ,Middle Aged ,Real-Time Polymerase Chain Reaction ,Sensitivity and Specificity ,Carcinoembryonic Antigen ,Up-Regulation ,MicroRNAs ,ROC Curve ,Predictive Value of Tests ,Area Under Curve ,Case-Control Studies ,Biomarkers, Tumor ,Humans ,Female ,Colorectal Neoplasms ,Aged - Abstract
The expression of miR-338-5p has been reported to be upregulated in colorectal cancer (CRC) tissues. Clinicopathological features indicate that miR-338-5p overexpression correlates with the metastatic status of CRC. This study was aimed to investigate the diagnostic value of serum miR-338-5p for CRC.Peripheral blood samples were collected from 210 participants, including 80 patients with CRC, 50 with colorectal polyps and 80 healthy controls. Serum miR-338-5p was quantified by quantitative reverse-transcription polymerase chain reaction. The area under the receiver operating characteristic curve (AUROC) was used to estimate the diagnostic value of miR-338-5p, carcinoembryonic antigen (CEA) and the combination of these two biomarkers.Serum miR-338-5p levels (fold change) in patients with CRC and colorectal polyps, and controls were 4.94 ± 1.13, 4.12 ± 0.75 and 3.07 ± 0.75, respectively. Significant differences were observed between the groups (P0.001). The AUROC of miR-338-5p was 0.923 (95% CI 0.882-0.964) and 0.845 (95% CI 0.792-0.898), respectively, for distinguishing CRC from healthy controls or from those without CRC. The AUROC of the combination of miR-338-5p and CEA was 0.932 (95% CI 0.882-0.964), with a sensitivity of 85%, a specificity of 88.8% at a cut-off value of 8.16.Circulating miR-338-5p may serve as a potential noninvasive diagnostic biomarker for detecting CRC. The combination of miR-338-5p and CEA exhibits the highest diagnostic value in our study.
- Published
- 2018
13. Serum microRNA signatures and metabolomics have high diagnostic value in hepatocellular carcinoma
- Author
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Xizhong Shen, Hai-Ning Liu, Hao Wu, Tao-Tao Liu, Yan-Jie Chen, Ling Dong, Yu-Jen Tseng, and Enkhnaran Bilegsaikhan
- Subjects
Oncology ,medicine.medical_specialty ,03 medical and health sciences ,0302 clinical medicine ,Metabolomics ,Internal medicine ,Medicine ,Diagnostic biomarker ,Serum microrna ,liver neoplasms ,microRNA ,business.industry ,Area under the curve ,Diagnostic test ,medicine.disease ,metabolomics ,meta-analysis ,diagnostic test ,030220 oncology & carcinogenesis ,Meta-analysis ,Hepatocellular carcinoma ,Biomarker (medicine) ,030211 gastroenterology & hepatology ,business ,Research Paper - Abstract
// Hai-Ning Liu 1, * , Hao Wu 1, * , Yan-Jie Chen 1, * , Yu-Jen Tseng 1 , Enkhnaran Bilegsaikhan 1 , Ling Dong 1 , Xi-Zhong Shen 1, 2 and Tao-Tao Liu 1 1 Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai 200032, China 2 Shanghai Institute of Liver Diseases, Zhongshan Hospital of Fudan University, Shanghai 200032, China * These authors have contributed equally to this work Correspondence to: Tao-Tao Liu, email: liu.taotao@zs-hospital.sh.cn Keywords: liver neoplasms; diagnostic test; meta-analysis; microRNA; metabolomics Received: August 06, 2017 Accepted: October 05, 2017 Published: November 01, 2017 ABSTRACT Background: Many new diagnostic biomarkers have been developed for hepatocellular carcinoma (HCC). We selected two methods with high diagnostic value, the detection of serum microRNAs and metabolomics based on gas chromatography/mass spectrometry (GC/MS), and attempted to establish appropriate models. Methods: We reviewed the diagnostic efficiencies of all microRNAs identified by previous diagnostic tests. Then we chose appropriate microRNAs to validate the diagnostic efficiencies, and determined the optimal combination. We included 66 patients with HCC and 82 healthy controls (HCs) and detected the expression of the microRNAs. GC/MS analysis was performed, and we used three multivariate statistical methods to establish diagnostic models. The concentration of alpha feto-protein (AFP) was determined for comparison with the novel models. Results: 82 published studies and 92 microRNAs were ultimately included in this systematic review. Seven microRNAs were selected for further validation of their diagnostic efficiencies. Among which, miR-21, miR-106b, miR-125b, miR-182 and miR-224 had a significantly different expression in HCC patients. The combination of miR-21, miR-106b and miR-224 had the highest area under the curve (AUC) at 0.950 with a sensitivity of 80.3% and a specificity of 92.7%. The GC/MS analysis exhibited an excellent diagnostic value and the AUC reached 1.0. In comparison, the AUC of the traditional biomarker, AFP, was 0.755. Conclusion: MicroRNAs and metabolomics shows promising potential as new diagnostic methods due to their high diagnostic value compared with traditional biomarkers.
- Published
- 2017
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