1. Hyperactivation of crosslinked lipases in elastic hydroxyapatite microgel and their properties.
- Author
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Jeon, Hyo Won, Lee, Jun Seop, Lee, Chan Hee, Kim, Dain, Lee, Hye Sun, and Hwang, Ee Taek
- Subjects
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CATALYTIC activity , *THERMAL stability , *LIPASES , *MESOPORES , *HYDROXYAPATITE , *BIOCATALYSIS - Abstract
Effective enzyme stabilization through immobilization is essential for the functional usage of enzymatic reactions. We propose a new method for synthesizing elastic hydroxyapatite microgel (E-HAp-M) materials and immobilizing lipase using this mesoporous mineral via the ship-in-a-bottle-neck strategy. The physicochemical parameters of E-HAp-M were thoroughly studied, revealing that E-HAp-M provides efficient space for enzyme immobilization. As a model enzyme, lipase (LP) was entrapped and then cross-linked enzyme structure, preventing leaching from mesopores, resulting in highly active and stable LP/E-HAp-M composites. By comparing LP activity under different temperature and pH conditions, it was observed that the cross-linked LP exhibited improved thermal stability and pH resistance compared to the free enzyme. In addition, they demonstrated a 156% increase in catalytic activity compared with free LP in hydrolysis reactions at room temperature. The immobilized LP maintained 45% of its initial activity after 10 cycles of recycling and remained stable for over 160 days. This report presents the first demonstration of a stabilized cross-linked LP in E-HAp-M, suggesting its potential application in enzyme-catalyzed processes within biocatalysis technology. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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