Search

Bakteriyorodopsin (BR), halofilik mikroorganizmalardan Halobacterium salinarum'da bulunan bir transmembran proteindir. Proton pompası olarak görev yapan bu protein ışık ile aktive olarak güneş enerjisinden enerji eldesini sağlamaktadır. Ayrıca farklı fiziksel koşullara dayanıklılık göstermesi BR'i ilgi çekici kılmaktadır. Birçok uygulama alanında önemli yere sahip olan BR yenilenebilir enerji alanında da kullanılmaktadır. Çalışmamızda Bakteriyorodopsin Temelli Biyomolekül Duyarlı Güneş Hücresi (BR-BSSC) elde etmek amacıyla Halobacterium salinarum DSM 671 suşu kullanılmıştır. H.salinarum DSM 671'den BR üretimi gerçekleştirilmiş ve üretim koşullarının optimizasyonu için Deney Tasarım programından yararlanılmıştır. Ardışık olarak deney tasarımı programlarının (Plackett-Burman, Central Composite Design) kullanımı sonucunda 95.3 mgL-1 olacak şekilde BR eldesi sağlanmıştır. Sulu ikili faz sistemi ile saflaştırılan BR kullanılarak Cam/FTO/TiO2/BR/MoO3/Au (D1) ve Cam/FTO/ZnO/BR/MoO3/Au (D2) olacak şekilde iki farklı cihaz elde edilmiştir. Bu cihazlardan D1'in verimliliği 0.8989, D2'nin verimliliği 0.8429 olarak hesaplanmıştır. Bacteriorhodopsin (BR) is a transmembrane protein that found in Halobacterium salinarum which is a halophilic bacterium. BR, after being activated by solar energy, acts as a proton pump and creates energy. Besides this property, BR, also shows resistance to various physical conditions and hence makes it more interesting. Among many important implementations, BR also takes part in renewable energy applications. In this research, H. salinarum DSM 671strain was used to obtain Bacteriorhodopsin Based Biomolecule Sensitized Solar Cell (BR-BSSC). For this purpose, BR was synthesized by H. salinarum DSM 671 and for optimization of growth conditions, an experimental design program was used. After sequential application of Plackett-Burman and Central Composite Design. BR was obtained in proportion of 95.3 mgL-1. Following aqueous-two-phase system purification, BR was used to produce two different devices named as Glass/FTO/TiO2/BR/MoO3/Au (D1) and Glass/FTO/ZnO/BR/MoO3/Au (D2). The efficiency of these devices were 0.8989 and 0.8429 respectively. 79

Demet ERDÖNMEZ (Aksaray Üniversitesi yazarı), Amaç: Bu çalışmada, Porphyromonas gingivalis ATCC 33277, Prevotella intermedia ATCC 25611 ve Aggregatibacter actinomycetemcomitans ATCC 43718 periodontal patojenlerine karşı gıdalarda bulunan fitokimyasallardan kuersetin, kurkumin, apigenin, pirogallol, gallik asit, luteolin ve hidroksitirozolün in vitro antimikrobiyal potansiyellerinin değerlendirilmesi amaçlanmıştır.Gereç ve Yöntem: Çalışmada agar kuyucuk difüzyon ve mikrodilüsyon yöntemleri kullanılarak fitokimyasalların antimikrobiyal potansiyelleri araştırılmıştır. Agar kuyucuk yönteminde fitokimyasalların antimikrobiyal aktiviteleri 3 mg/ml, mikrodilüsyon yönteminde ise 3, 1.5, 0.75, 0.375, 0.187, 0.093, 0.046, 0.023 ve 0.011 mg/ml konsantrasyonlarda incelenmiştir. Pozitif ve negatif kontrol olarak amoksisilin (20 µg/disk ve 0.04-0.0015 mg/ml) ve DMSO (, Objective: In this study, it is aimed to investigate the antimicrobial potential of the dietary phytochemicals including quercetin, curcumin, apigenin, pyrogallol, gallic acid, luteolin and hydroxytyrosol against the periodontal pathogenic bacteria including Porphyromonas gingivalis ATCC 33277, Prevotella intermedia actinomycetemcomitans ATCC 43718.ATCC and Aggregatibacter Material and Methods: The antimicrobial potential of the dietary phytochemicals was investigated by using agar well diffusion and microdilution methods. The antimicrobial activities of the phytochemicals were both studied at a concentration of 3 mg/ml and also in concentrations of 3, 1.5, 0.75, 0.375, 0.187, 0.093, 0.046, 0.023 and 0.011 mg/ml using microdilution methods, respectively. Amoxicillin (20 µg/disc, 0.04-0.0015 mg/ml) and DMSO (≤10%) were tested as positive and negative controls, respectively.Results: According to the results of this study, most of the phytochemicals had antimicrobial activity aganist all of the bacteria. The most effective phytochemical against pathogens was quercetin. The phytochemicals with the strongest antimicrobial potential were determined as quercetin, curcumin, luteolin, apigenin and gallic acid. In our study DMSO (≤4%, v/v) showed no antimicrobial effect on the pathogens.Conclusion: In addition to the usage of antibiotics in the treatment of periodontal diseases, the consumption of the phytochemicals including especially curcumin, quercetin, luteolin, gallic acid and apigenin may be appropriate and helpful. Therefore, it is suggested that more detailed in vivo research about the inhibition mechanisms of the periodontal pathogens by potent phytochemicals should be performed and, further in vivo studies should be carried out to detect effective usage of these phytochemicals