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In each of two experiments, two groups of rats were exposed to three flavoured solutions: A (citric acid), B (salt), and AX (a compound of citric acid and saccharin). Both experiments used a between-subjects design in which a paired group received presentations of A followed by B, alternating with presentations of AX (i.e., A → B/AX), and an unpaired group received alternating presentations of A, B, and AX (i.e., A/B/AX). This arrangement was expected to establish X as an inhibitor of B in group paired but not in group unpaired. In Experiment 1, after preexposure all subjects received a single presentation of an XB compound, then experienced sodium depletion, and were tested for their consumption of X, which was greater in group unpaired than in group paired. In Experiment 2, after preexposure, all subjects received four presentations of a new flavour, C, in compound with B and subsequently, under sodium depletion, were tested for consumption of XC. Intake of the XC compound was less in group paired than in group unpaired. These results suggest that, in group paired, X acquired an inhibitory relationship with B both retarding the acquisition of an excitatory association with B (retardation test, Experiment 1) and reducing the response to a new stimulus, C, strongly associated to B (summation test, Experiment 2). These results provide direct evidence of inhibition between two neutral stimuli and, therefore, of inhibitory sensory preconditioning.