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A mastite em ovelhas da raça Santa Inês apresenta-se como um problema de grande proporção e gravidade e é dificilmente tratada com sucesso. O objetivo deste estudo foi caracterizar aspectos clínicos, epidemiológicos e etiológicos da mastite clínica em ovelhas de corte criadas no norte do Paraná. O presente estudo foi realizado entre os meses de outubro de 2009 a setembro de 2010 envolvendo 54 rebanhos de ovinos de corte de diferentes raças. Durante as visitas às propriedades, um questionário foi preenchido com a finalidade de caracterizar o problema. Setenta ovelhas com mastite clínica foram examinadas e amostras de secreção láctea foram colhidas para exame microbiológico. A mastite foi considerada um problema relevante em 39 propriedades (72,3%), com frequência média de 6,74%. Casos crônicos e agudos de mastite foram observados em 69% e 31% das ovelhas examinadas, respectivamente. Em ambos os casos, a mastite flegmonosa foi a forma mais encontrada (65,5% dos casos). O agente etiológico mais prevalente foi Staphylococcus coagulase negativo (54,5%), seguido por S. aureus e A. pyogenes (11,5% cada). Mannheimia haemolytica foi isolada em dois casos. Sistema de criação não extensivo e raça Santa Inês foram identificados como fatores de risco para o desenvolvimento de mastite clínica. Secagem das fêmeas após 120 dias de lactação e separação de fêmeas doentes do rebanho foram associadas com menor ocorrência da doença. Recomenda-se a limpeza adequada das instalações e a secagem mais tardia, principalmente em rebanhos Santa Inês.

Chlamydophila abortus é o agente etiológico do aborto epizoótico bovino, cujas manifestações clínicas mais freqüentes são aborto, nascimento de bezerros prematuros e de animais fracos, natimorto e repetição de cio em intervalos irregulares. O objetivo deste trabalho foi estimar a prevalência de anticorpos anti-Chlamydophila spp. em fêmeas bovinas de propriedades rurais com histórico de aborto, selecionadas dentro do delineamento amostral do Plano Nacional de Controle e Erradicação da Brucelose e Tuberculose no estado do Paraná. Foram testadas pela prova de fixação de complemento 3.102 amostras de soro de fêmeas bovinas (idade > 24 meses), provenientes de 373 propriedades. Ao total, 44 (1,42%) animais foram positivos com títulos > 32. A prevalência de focos foi de 8,82% (6,15%-12,17%). Animais confinados ou semi-confinados (OR=3.339, P=0.004), propriedade com menos de 35 matrizes (OR=3.339, P=0.017), presença de produtos do aborto na pastagem (OR=2.372, P=0.037) e aluguel de pasto (OR=3.398, P=0.006) foram considerados fatores de risco para Chlamydophila spp. A infecção por Chlamydophila spp. acometeu um número pequeno de animais, oriundos de propriedades com histórico de aborto. A importância deste agente como causa de aborto em bovinos no estado do Paraná, se existir, é muito pequena.Chlamydophila abortus is a recognized cause of bovine epizootic abortion. Abortion, premature birth and weak lamb/calf, stillbirth and repeat breeding in irregular intervals are the most frequent disease manifestations. The complement fixation test is the recommended by the World Organization of Animal Health (OIE) for Chlamydophila spp. serologic diagnosis. The aim of this study was estimate the prevalence of antibodies against Chlamydophila spp. in cattle herds with abortion, selected inside the sampling design of National Program of Control and Erradication of Brucellosis in Paraná state. Serum samples of 3,102 cows (age > 24 months) from 373 herds were analyzed by complement fixation test. Totally, 44 (1.42%) animal were positive with titers > 32. The seroprevalence of Chlamydophila spp. in the herds was 8.82% (6.15%-12.17%). Four variables were associated with seroprevalence for Chlamydophila spp. in the final model of logistic regression: confined or semi-confined breeding (OR=3.339, P=0.004), farms with less than 35 cows (OR=3.339, P=0.017), abortion in the pasture (OR=2.372, P=0.037) and pasture rent (OR=3.398, P=0.006) were risk factors for Chlamydophila spp. This bacterium infected a small number of cattle from herds with abortion in Paraná state. Chlamydophila spp impact as abortion cause is reduced in this state.

O objetivo deste estudo foi determinar a prevalência de rebanhos positivos (focos) e identificar os fatores de risco que possam estar associados com a infecção pelo herpesvírus bovino 1 (BoHV-1) em rebanhos bovinos com atividade reprodutiva, na região Oeste do Estado do Paraná. O delineamento estatístico, amostras de soro e informações referentes às propriedades foram as empregadas para o estudo da brucelose bovina no Estado do Paraná dentro do contexto do Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose. Foram avaliadas 1930 fêmeas com idade igual ou superior a 24 meses, provenientes de 295 rebanhos não vacinados contra o BoHV-1. Para o diagnóstico sorológico da infecção pelo BoHV-1, foi utilizado um ensaio imunoenzimático (ELISA) indireto. Em cada propriedade foi aplicado um questionário epidemiológico, afim de obter informações epidemiológicas e práticas de manejo empregadas. Dos 295 rebanhos analisados, 190 foram considerados positivos para o BoHV-1, com a prevalência de rebanhos de 64,41% (I.C.95% = 58,65-69,87%). As variáveis consideradas fatores de risco para a infecção pelo BoHV-1 na análise de regressão logística multivariada foram: i) número (>23) fêmeas com idade >24 meses (OR=2,22; IC: 1,09-4,51); ii) compra de reprodutores (OR=2,68; IC: 1,48-4,82); iii) uso de pastagens comuns (OR=5,93; IC: 1,31-26,82); iv) histórico de abortamento nos últimos 12 meses (OR=2,37; IC: 1,09-5,16); v) presença de animais silvestres (OR=8,86; IC: 1,11-70,73). Estes resultados indicam que a infecção pelo BoHV-1 está amplamente distribuída na região estudada e que fatores relacionados às características das propriedades e ao manejo estão associados à infecção.The aim of this study was to determine the prevalence of positive herds and to identify the risk factors for BoHV-1 infection in breeding cattle herds in the Western region of Parana State. The statistic delineation, the serum samples and information regarding the selected farms, were the same employed in the study of bovine brucellosis for Parana State in the context of National Program for Control and Eradication of Brucellosis and Tuberculosis. The sampling was performed in two stages. Blood samples were collected from 1930 females aged >24 months from 295 non vaccinated herds. Serum samples were tested for antibodies against BoHV-1 using an indirect ELISA. The epidemiological questionnaire was applied on all the selected farms and aimed to obtain epidemiological data. Hundred ninety of the 295 herds were positive for BoHV-1, presenting prevalence of positive herds of 64.41% [58.65-69.87%]. The number (>23) of females aged 24 months (OR=2.22; IC: 1.09-4.51), purchased cattle (OR=2.68; IC: 1.48-4.82), use of common grass (OR=5.93; IC: 1.31-26.82), history of abortion in the last 12 months (OR=2.37; IC: 1.09-5.16) and presence of wildlife animals (OR=8.86; IC: 1.11-70.73) were identified as risk factors for BoHV-1 infection in the multivariate logistic regression. These results indicate that BoHV-1 infection is widespread in the studied region and that factors related to the herd characteristic and management are associated with the infection.

Foram examinadas 713 vacas de três rebanhos leiteiros localizados na região norte do Estado do Paraná e sudoeste do Estado de São Paulo, das quais 137 apresentaram mastite. Nas três propriedades foram detectados oito animais (1,12%) com mastite clínica por Mycoplasma bovis. Destes animais, quatro tratados com oxitetraciclina e tilosina e três com enrofloxacina, não responderam ao tratamento e foram descartados no decorrer da lactação. Uma vaca medicada com enrofloxacina recuperou quase que totalmente a secreção láctea mas a eliminação de M. bovis persistiu por toda lactação. Esta vaca apresentou cura bacteriológica na lactação seguinte. O descarte dos animais positivos, monitora-mento bacteriológico e a aplicação correta das medidas de prevenção para as mastites contagiosas controlaram a disseminação de M. bovis nos rebanhos.In this study 713 cows were examined. The animals were from three dairy farms in northern Paraná and the southwest of the State of São Paulo. From these cows, 137 had mastitis. On the three farms, 8 cows (1.12%) with Mycoplasma bovis mastitis were detected. Four were treated with tylosin and oxytetracyclin and three with enrofloxacin. There was no response to the treatments, and these animals were culled during the lactation period. One cow treated with enrofloxacin almost totally recovered milk production, but elimination of M. bovis continued during the lactation, and there was no bacteriological cure. This cow had a normal milk production in the next lactation period, without elimination of M. bovis. Culling of positive animals, the bacteriological study and correct application of preventive practices for contagious mastitis controlled the dissemination of M. bovis to other animals.

De um total de 158 cães examinados, através de exame parasitológico de fezes ou necropsia, 70 (44,30%) mostraram-se infectados por Toxocara canis, predominando a infecção nos animais com até 6 meses de idade. Através do exame de amostras de terra de localidades públicas utilizadas por crianças para diversão, demonstrou-se a presença de ovos de Toxocara sp. em 60% das amostras, evidenciando a possibilidade de ocorrer infecção humana, ingestão de ovos larvados do helminto.Out of 158 dogs submitted to coprological examination or necropsy, 70 (44.30%) were found to be infected by Toxocara canis, the prevalence being higher among puppies with and up to 6 months of age. Out of 15 soil samples taken from public places atended by children, ova of Toxocara species were found in 60% indicating the possibility of transmission of toxocaral infection to humans through ova containing larvae.

ABSTRACT: Mastitis represents an important health problem for Santa Inês breed, causing losses to the producer, due to loss of ewes or the decrease in weight gain of lambs. The aim of this work was to assess the health of the mammary gland of Santa Inês ewes at the drying and puerperium and to investigate the efficacy of a dry-off therapy with gentamicin. In this study, 64 ewes were divided in a control group (GC) and treatment group (GT), and the health of the mammary gland was assessed at the drying and puerperium. The GT ewes received 250mg of gentamicin (Gentocin® DryCow/Schering-Plough, product indicated for use in dairy cows) in each mammary half. For diagnosis, clinical examination, California Mastitis Test, somatic cell count and milk culture was performed. In the GC, of the 45 (70.3%) healthy mammary halves at the drying, 12 developed subclinical mastitis and nine clinical mastitis at the puerperium. In the GT, among 51 (79.7%) healthy mammary halves at the drying, six developed subclinical mastitis and 11 clinical mastitis at the puerperium. No association was observed between treatment and the occurrence of mastitis at puerperium. Of the 19 (29.7%) mammary halves of the GC that presented subclinical mastitis at the drying, three remained with subclinical mastitis and five developed clinical mastitis at the puerperium. In the GT, of the 13 (20.3%) mammary halves that had subclinical mastitis at the drying, four remained with subclinical mastitis and four developed clinical mastitis. No association was observed between treatment and cure or persistence of mastitis at the puerperium. The main microorganisms isolated, at the drying and puerperium, from animals with subclinical or clinical mastitis were Staphylococcus spp., predominantly coagulase negative Staphylococcus (CSN). At the puerperium, 29 cases of clinical mastitis occurred, 19 with isolation, where 10 were CNS and six S. aureus. Mannheimia haemolytica was isolated in one case of subclinical mastitis and other of clinical mastitis. News protocols and different ways of handling at drying and at puerperium must be investigated.

In order to improve the microbiological quality of Brazilian milk and dairy products some measures have been implemented in the milk-producing sector throughout the years, such as milk refrigeration and bulk collection. Refrigeration is a very efficient procedure, however allows psychrotrophics multiplication, which are largely found in milk produced in poor hygienic conditions. To assure and improve the microbiologic quality of milk turns out to be necessary the implementation of hygienic measures, known as Good Manufacturing Practices (GMP). In this study, several hygienic practices were tested in the main contamination points in milk production, previously determined: teats (different chlorine concentrations pre-dipping), cans and bulk tanks (hygienic techniques) and residual water (elimination). The recommended practices were picked up considering their efficiency in microbial reduction, practiced and economic viability. When the practices were all applied, reduction in microbial counts was observed. After 48 hours the final and refrigerated milk showed a reduction of 11.95x106 CFU/mL to 12.48x103 CFU/mL of mesophilic aerobes, and a reduction of 18.10x106 CFU/mL to 5.38x103 CFU/mL of psychrotrophics. The results show that the procedures proposed by LIPOA is efficient, viable and easily adopted by milk farmers, representing an important alternative in producing high quality milk.Buscando a melhoria da qualidade microbiológica de leite e derivados produzidos no Brasil, nos últimos anos algumas medidas vêm sendo implantadas na produção leiteira como a refrigeração e coleta a granel. A refrigeração é um procedimento eficiente no controle de aeróbios mesófilos, mas permite multiplicação de psicrotróficos, encontrados em altas contagens em leite produzido com pouca higiene. Para garantia e melhoraria da qualidade microbiológica do leite é necessária a implantação de medidas higiênicas, conhecidas como Boas Práticas de Produção (BPP). Neste estudo, diversas práticas higiênicas foram testadas nos principais pontos de contaminação na produção leiteira, previamente determinados: tetos (pré-dipping com diversas concentrações de cloro), latões e refrigeradores (técnicas de higienização), e água residual (eliminação). As práticas recomendadas foram escolhidas quanto à eficiência na redução de microrganismos, praticidade e viabilidade econômica. Quando as práticas foram aplicadas conjuntamente, obteve-se reduções consideráveis nas contagens microbianas. Após 48 horas de refrigeração do leite, as contagens foram reduzidas de 11,95x106 CFU/mL para 12,48x103 CFU/mL de aeróbios mesófilos, e de 18,10x106 CFU/mL para 5,38x103 CFU/mL de psicrotróficos. Os resultados mostram que as práticas propostas são eficientes, viáveis e facilmente adotáveis por produtores leiteiros, representando uma importante alternativa na produção de leite com alta qualidade.

The aim of this study was to determine the prevalence of anti-Leptospira spp. antibodies and the risk factors for Leptospira spp. infection in breeding cattle herds in the south central region of Paraná state. It was based on the statistic delineation/serological samples and information regarding the selected farms employed in the study of bovine brucellosis for Paraná state in the context of National Program for Control and Eradication of Brucellosis and Tuberculosis. A total of 1.880 females aged >24 months from 274 non vaccinated herds were studied. Serum samples were tested for antibodies against Leptospira spp. using microscopic agglutination test (MAT) with 22 Leptospira serovars. The epidemiological questionnaire was applied on all the selected farms and aimed to obtain epidemiological data. Hundred eighty one of 274 herds were positive for Leptospira spp./presenting prevalence of positive herds of 66.06% (IC95%=60.12-71,65%). Presence of >43 cattle (OR=3.120; IC=1.418-6.867)/animal purchase (OR=2.010; IC=1.154-3.500)/rent of pastures (OR=2.925; IC=1.060-8.068) and presence of maternity paddock (OR=1.981; IC=1,068-3,676) were identified as risk factors for leptospirosis due to any serovar in the multivariate logistic regression. Risk factors for leptospirosis due to serovar Hardjo were presence of >43 cattle (OR=3.622; IC=1.512-8,677)/animal purchase (OR=3.143; IC=1.557-6.342)/rent of pastures (OR=4.070; IC=1.370-12.087) and presence of horses (OR=2.981; IC=1.321-6.726). These results indicate that Leptospira spp. infection is widespread in the south central region of Paraná state and that factors related to the herd characteristic and management are associated with the infection.

This paper presents a feasibility study of an automated pick-and-place process for ultrathin chips on a standard automatic assembly machine. So far, scientific research about automated assembly of ultrathin chips, with thicknesses less than 50 µm, is missing, but is necessary for cost-effective, high-quantity production of system-in-foil for applications in narrow spaces or flexible smart health systems applied in biomedical applications. Novel pick-and-place tools for ultrathin chip handling were fabricated and a process for chip detachment from thermal release foil was developed. On this basis, an adhesive bonding process for ultrathin chips with 30 µm thickness was developed and transferred to an automatic assembly machine. Multiple ultrathin chips aligned to each other were automatically placed and transferred onto glass and polyimide foil with a relative placement accuracy of ±25 µm.

Erlotinib with bevacizumab showed promising activity in recurrent nonsquamous (NS) nonsmall cell lung cancer (NSCLC). The INNOVATIONS study was designed to assess in first-line treatment of unselected cisplatin-eligible patients this combination compared to cisplatin, gemcitabine and bevacizumab. Stage IIIB/IV patients with NS-NSCLC were randomised on erlotinib (150 mg daily) and bevacizumab (15 mg·kg(-1) on day 1, every 3 weeks) (EB) until progression, or cisplatin (80 mg·m(-2) on day 1, every 3 weeks) and gemcitabine (1250 mg·m(-2) on days 1 and 8, every 3 weeks) up to six cycles and bevacizumab (15 mg·kg(-1) on day 1, every 3 weeks) (PGB) until progression. 224 patients were randomised (EB n=111, PGB n=113). The response rate (12% versus 36%; p<0.0001), progression-free survival (median 3.5 versus 6.9 months; hazard ratio (HR) 1.85, 95% CI 1.39-2.45; p<0.0001) and overall survival (median 12.6 versus 17.8 months; HR 1.41, 95% CI 1.01-1.97; p=0.04) clearly favoured PGB. In patients with epidermal growth factor receptor mutations (n=32), response rate, progression-free survival and overall survival were not superior with EB. Platinum-based combination chemotherapy remains the standard of care in first-line treatment of unselected NS-NSCLC. Molecular targeted approaches strongly mandate appropriate testing and patient selection., (Copyright ©ERS 2015.)

The course of older patients with hip fractures is often complicated by infections and delirium. Accurate care and high suspicion for these complications are essential, since these conditions are associated with an increase in mortality, length of hospital stay and nursing home placement, poorer mobility, and functional decline. Because of immunosenescence and higher infection rates, older patients need specific care, immediate diagnosis, and treatment of infections. Numerous guidelines of various medical societies outline the management of nosocomial infections, but there is a need of an individualized treatment plan because of comorbidities and polypharmacy. Hygiene measures have first priority to reduce the rate of infections. Treatment of geriatric syndromes like malnutrition, exsiccosis, gait disorders, falls, delirium, urine incontinence, and organ insufficiency are as important as immunization against pneumococci and influenza. Advanced age, cognitive impairment, hearing loss, peripheral vascular disease, prior delirium episodes, sight disorders, and polypharmacy are established risk factors for delirium; thus, older people with several chronic diseases are prone to delirium. A multifactorial approach, comprising standardized screening, oxygen support, intravenous fluid administration and augmented nutrition, monitoring of vital signs, pain treatment, optimized medication, and modification in perioperative management, significantly reduces delirium incidence during hospitalization for hip fracture. An interdisciplinary approach between surgeons and geriatricians may warrant optimized satisfaction of patients' needs.

Hip fracture in older patients is a major health concern. 20-25 % of hip fracture patients will die in the first year after the trauma (Lane, Clin Orthop Relat Res 471(8):2711, 2013). Postoperative venous thrombosis and gastrointestinal stress-ulcer bleeding are frequent complications with a high case-fatality rate particularly in older patients. Thromboprophylaxis and stress ulcer prophylaxis are important and well established measures to decrease postoperative complications and the mortality rate in this high-risk population.The working group on orthogeriatrics of the Austrian Society on Geriatrics and Gerontology (ÖGGG) is composed of geriatricians who work as trauma surgeons, internists, anaestesists and nurses. A thorough literature search was done, using the terms "orthogeriatrics" and "hip fracture" in combination with "stress ulcer", "gastrointestinal bleeding" and "thrombosis", "thromboprophylaxis". The data was collected, discussed and evaluated in several adjustment meetings of the group and summarized in this article.

Background: Upper airway inflammation has been previously demonstrated in obstructive sleep apnea (OSA). However, investigation has been hampered by the necessity of invasive tissue biopsies., Objective: To evaluate the pharyngeal lavage (PHAL) as a new tool to analyze mucosal inflammation in the pharynx of patients with sleep-related disordered breathing., Patients and Methods: 36 patients with a diagnosis of OSA, 14 patients with heavy snorer syndrome (HS) or body position dependent OSA (bd-OSA), and 14 healthy volunteers underwent PHAL. Inflammatory cell counts were compared., Results: Neutrophils were the predominant cells in PHAL in all groups (94.3% ± 0.7%, 98.5% ± 0.6%, 94.3% ± 0.7%, and 96.2% ± 1.4%). OSA patients had significantly increased numbers of lymphocytes (3.2% ± 0.4%) compared to bd-OSA/HS and controls group (0.5% ± 0.1% and 0.6% ± 0.2%, respectively; P<0.05). Patients with moderate to severe OSA had significantly higher numbers of lymphocytes compared to patients with mild OSA (P<0.05)., Conclusions: Data from this study suggest that PHAL is a feasible tool to investigate upper airway inflammation in OSA. In addition, PHAL demonstrates lymphocytic inflammation of the pharynx in OSA patients. Future studies are warranted to evaluate whether PHAL can be used to monitor disease and whether lymphocytic inflammation is affected by OSA treatment.

Background: Individual flap design and minor donor site morbidity are main criteria in the treatment of facial defects after tumour surgery. Microvascular perforator flaps seem to follow these criteria well. In the following study our experiences with microvascular anterolateral thigh perforator flaps (ALTPF) in reconstruction of the face following ablative tumour surgery are described and discussed in comparison to the present literature., Patients and Methods: In 19 patients with squamous cell carcinomas of the floor of the mouth (8), the cheek (6) or the mandible (5) of stadium T3 or T4 ablative tumour surgery followed by reconstruction was performed. For covering the soft tissue defects 19 ALTPF were used. In the five patients with carcinomas of the mandible a microvascular iliac crest transplant was combined with the anterolateral thigh perforator flap for complete chin reconstruction., Results: In one patient an intraoperative dissection of the perforator vessels happened. In all other patients surgery and postoperative period was free of complications. Five patients had minor second surgery for aesthetic of functional reasons. At the end there were good aesthetic and functional results in every patient., Conclusions: The anterolateral thigh perforator flap is of great advantage in reconstruction of the face after tumour surgery. Individual designing, central and save perfusion, easy to be controlled, and a low incidence of donor site morbidity are their main advantages.